Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,302,836 G→C 6.5% intergenic (+23/‑63) oppA → / → oppB oligopeptide ABC transporter periplasmic binding protein/oligopeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,302,8360GC 6.5% 183.8 / 5.0 62intergenic (+23/‑63)oppA/oppBoligopeptide ABC transporter periplasmic binding protein/oligopeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (28/29);  new base C (2/2);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.78e-01

TTCGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTC  >  NC_000913/1302687‑1302985
                                                                                                                                                     |                                                                                                                                                     
ttCGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAgtg                                                                                                                                                       <  1:576804/150‑1 (MQ=255)
      cATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGGGGGGTGGTGGCTATACCGGCAAAGAGCCGCTGGATAATACCTATACCCGGAATATGTACATTGGGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGc                                                                                                                                                 <  2:173542/150‑1 (MQ=255)
         tGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGAGCAGGACACTCCTGcccc                                                                                                                                              <  1:1230782/150‑1 (MQ=255)
          gTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCa                                                                                                                                             <  2:175757/150‑1 (MQ=255)
          gTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGAGCAGGACACTCCTGCCCCa                                                                                                                                             <  2:151905/150‑1 (MQ=255)
             ccTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACgg                                                                                                                                          <  1:947758/150‑1 (MQ=255)
             ccTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACgg                                                                                                                                          <  2:616772/150‑1 (MQ=255)
             actctttATTACTACGTGAATGCGCGTCTTTTGAAACCGTTGGGTGGTGGCAATACCGGCATACATCCGCTGGATAATACCTATACCAGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGGCCTGCTCCACgg                                                                                                                                          <  2:25718/146‑1 (MQ=255)
               tGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGgtg                                                                                                                                        <  2:572462/150‑1 (MQ=255)
               tGTTTATTACTACGGGAAGGCGCGTCTGGTGAAACCGTGGGTGGGTGGCTAGACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGGGAAGCACTAATGGCAATACGTGGGGCAGGAGTGGCCTGCTCCACGgtg                                                                                                                                        <  1:540333/150‑1 (MQ=255)
                    attaCTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGa                                                                                                                                   <  2:253/150‑1 (MQ=255)
                          acGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTa                                                                                                                             <  1:51814/150‑1 (MQ=255)
                           cGTGAATGCGCGTCTGGTGAAACCGTGGGTTTGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTAt                                                                                                                            <  1:303420/150‑1 (MQ=255)
                            gTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATc                                                                                                                           <  2:1073899/150‑1 (MQ=255)
                                   cgcgTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTAc                                                                                                                    >  1:698654/1‑150 (MQ=255)
                                   cgcgTCTGGTGAAACCGTGGGTGGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTCAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCAGGGTGACTGATATATATCTCATTAc                                                                                                                    >  2:632891/1‑150 (MQ=255)
                                               aaCCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAgg                                                                                                        >  2:8734/1‑150 (MQ=255)
                                               aaCCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCCGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAgg                                                                                                        >  2:265629/1‑150 (MQ=255)
                                                 ccGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGcc                                                                                                      >  2:1199433/1‑150 (MQ=255)
                                                      ggTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAg                                                                                                 >  2:1181399/1‑150 (MQ=255)
                                                              gCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGTGAAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCa                                                                                         >  2:1196119/1‑150 (MQ=255)
                                                               cTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGATTATGTACATTGTGAAGCACAAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCaa                                                                                        <  1:428862/150‑1 (MQ=255)
                                                                 ataCCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATg                                                                                      <  1:401425/150‑1 (MQ=255)
                                                                 ataCCGGCAAAGATCCGCGGGATAATACCTATACCCGGAATATGTACAGTGTGAAGCACTAATGGCAATACGTGGTGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATg                                                                                      <  2:205379/150‑1 (MQ=255)
                                                                  taCCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGt                                                                                     >  1:951319/1‑150 (MQ=255)
                                                                      ggCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTaaa                                                                                 >  1:514179/1‑150 (MQ=255)
                                                                      ggCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTaaa                                                                                 >  1:412683/1‑150 (MQ=255)
                                                                        cAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAAt                                                                               >  1:1138776/1‑150 (MQ=255)
                                                                        cAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCATGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAAt                                                                               >  1:1229471/1‑150 (MQ=255)
                                                                         aaaGATCCGCTTGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACTGAACGCACAGGCCAGAAGGTAGGGCAATGTTAAAAtt                                                                              <  2:1036814/150‑1 (MQ=255)
                                                                         aaaGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAAtt                                                                              >  2:234498/1‑150 (MQ=255)
                                                                          aaGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAAttt                                                                             >  1:71262/1‑150 (MQ=255)
                                                                            gATCCGCTGGATAAAACCTATACCCGGAATATGTACATTGTGAAGCACAATTGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTAt                                                                           <  2:1247557/150‑1 (MQ=255)
                                                                              tCCGCTGGATAATACCTATACCCGGAATATGTACATTGGGAACCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTc                                                                         <  1:62203/150‑1 (MQ=255)
                                                                               ccGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCt                                                                        <  1:302822/150‑1 (MQ=255)
                                                                                cGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTa                                                                       >  2:104968/1‑150 (MQ=255)
                                                                                 ccTGGATAATACCTAAACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGATGTTCCGTCGCCACGGGGTCTTAATTTTAACGCATTACATAAGGCACATGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTAc                                                                      <  2:709570/149‑1 (MQ=255)
                                                                                     gATAATACCTATACCCGTAATCTGAACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCGCGACGGTGTCTGATTTATATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCg                                                                  <  1:1212951/150‑1 (MQ=255)
                                                                                      atactaCCTATACCCGGAATATGTACATTGTGAAGCACTAATGTCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCCCAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGc                                                                 <  2:477658/150‑1 (MQ=255)
                                                                                       taataCCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCt                                                                >  1:39980/1‑150 (MQ=255)
                                                                                         ataCCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGt                                                              >  1:8156/1‑150 (MQ=255)
                                                                                            ccTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGTCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTg                                                           >  1:917455/1‑150 (MQ=255)
                                                                                               ataCCCGGAATATGTACATTGTGAAGCACTAATGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAg                                                       <  1:1037274/150‑1 (MQ=255)
                                                                                                        atatGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCg                                               <  2:490390/150‑1 (MQ=255)
                                                                                                         tatGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGa                                              >  2:1276226/1‑150 (MQ=255)
                                                                                                          atGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGAc                                             <  2:1221129/150‑1 (MQ=255)
                                                                                                           tGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACg                                            >  1:345405/1‑150 (MQ=255)
                                                                                                           tGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACg                                            <  1:1199433/150‑1 (MQ=255)
                                                                                                            gTACATTGTGAAGCACTACTGGCAATAAGTGGGGCTGGAGTGTCGTGCTCCAGGGTGGCTGATTTTTATCGCATTACAGAAGGCCCACGCCAGAAGGTAGGGCAATGTTAAATTTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGc                                           <  2:181875/150‑1 (MQ=255)
                                                                                                            gTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATATTTATCGCATTACAGAAGGCACAGGCAAGAAGGTAGGGAAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATGCCGACGc                                           >  1:979018/1‑150 (MQ=255)
                                                                                                               cATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCGACGTCGCTGTCTGGAAGCGATTCCGACGCtat                                        >  2:960523/1‑150 (MQ=255)
                                                                                                                 ttGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCtattt                                      >  1:1006547/1‑150 (MQ=255)
                                                                                                                 ttGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCAACGGTGTCTGATTTTTATCGCATTACAGCAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATACCGACGCtattt                                      >  2:1001721/1‑150 (MQ=255)
                                                                                                                       aGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCtt                                >  1:890714/1‑150 (MQ=255)
                                                                                                                             aaTGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACt                          <  1:687556/150‑1 (MQ=255)
                                                                                                                             aaTGGCAATACGTGGGGCAGGAGTGTCCTGCACCACGGTGTCTGATGTTGATCGCATTTAAGAAGGCCCAGGCCAGAAGGTAGGGCAATGATAAAATTTATTCTCCGTCGCTGTCTGGAAGCGAGTCCGAGGATATTTTTTGTTATTACt                          >  2:841364/1‑150 (MQ=255)
                                                                                                                                ggCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAACGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTAtt                       <  2:43994/150‑1 (MQ=255)
                                                                                                                                   aaTACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCg                    <  1:118892/150‑1 (MQ=255)
                                                                                                                                      ccGTGGAGCAGGACACTCCTGCCCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTc                 >  2:162907/2‑150 (MQ=255)
                                                                                                                                      ccGTGGAGCAGGACACTCCTGCCCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTc                 >  2:133071/2‑150 (MQ=255)
                                                                                                                                          ggggCAGGAGTGTCCTGCGCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACCGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTa             <  1:122836/150‑1 (MQ=255)
                                                                                                                                             gCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTatga          >  1:859203/1‑150 (MQ=255)
                                                                                                                                             gCAGGAGTGTCCTGCTCCACGGTGTCTGATATTTATCGCATTACAGAAGGCCCAGGACTGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAATCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTATTTttga          >  2:401438/1‑150 (MQ=255)
                                                                                                                                                     gtCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGTAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTc  <  1:234498/150‑1 (MQ=255)
                                                                                                                                                     gtCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTGTTTATGATGCGCCTc  >  2:522456/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTC  >  NC_000913/1302687‑1302985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: