BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,406,129	T→C	intergenic (+144/‑61)	purA → / → nsrR	adenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,406,129	0	T	C	100.0%	488.2 / NA	136	intergenic (+144/‑61)	purA/nsrR	adenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (68/68); total (68/68)

Only 100 of 136 total aligned reads displayed.

AATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTT  >  NC_000913/4405984‑4406274

 aTTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCtgtg                                                                                                                                                >  2:460960/1‑149 (MQ=255)

      ggTACGCCTGGCAGAGATTTTGCCGGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTgg                                                                                                                                           <  2:39190/149‑1 (MQ=255)

         aCGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGttt                                                                                                                                        <  2:972545/149‑1 (MQ=255)

          cGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTa                                                                                                                                       <  2:346332/149‑1 (MQ=255)

           gCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTat                                                                                                                                      <  1:1071406/149‑1 (MQ=255)

             cTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTatca                                                                                                                                    <  1:375344/149‑1 (MQ=255)

              tGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTatcat                                                                                                                                   <  2:338683/149‑1 (MQ=255)

                gCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTAtcatca                                                                                                                                 >  1:314061/1‑149 (MQ=255)

                 cAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCaa                                                                                                                                <  1:582831/149‑1 (MQ=255)

                 cAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCaa                                                                                                                                <  1:468312/149‑1 (MQ=255)

                    atatTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAatat                                                                                                                             >  1:336130/1‑149 (MQ=255)

                       ttttGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCACGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATaaa                                                                                                                          <  1:876263/149‑1 (MQ=255)

                        tttGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAAt                                                                                                                         >  1:1039912/1‑149 (MQ=255)

                        aatatctgccAGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAAt                                                                                                                         >  2:636384/6‑149 (MQ=255)

                            cctgccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑t                                                                                                                    >  2:136375/1‑149 (MQ=255)

                             ctgccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑tt                                                                                                                   <  2:34381/149‑1 (MQ=255)

                                  gggCGAACTGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTc                                                                                                              <  2:625040/149‑1 (MQ=255)

                                  gggCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTc                                                                                                              <  1:1109898/149‑1 (MQ=255)

                                   ggCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTcc                                                                                                             >  2:556592/1‑149 (MQ=255)

                                    gCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTccc                                                                                                            >  1:560711/1‑149 (MQ=255)

                                      gAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGa                                                                                                          >  1:1143549/1‑149 (MQ=255)

                                          aGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTc                                                                                                      >  1:492683/1‑149 (MQ=255)

                                               gATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCttt                                                                                                 >  2:867871/1‑149 (MQ=255)

                                                aTACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCtttt                                                                                                >  1:921903/1‑149 (MQ=255)

                                                aTACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCtttt                                                                                                >  2:886490/1‑149 (MQ=255)

                                                aTACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCtttt                                                                                                >  2:167593/1‑149 (MQ=255)

                                                aTACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCtttt                                                                                                <  2:521935/149‑1 (MQ=255)

                                                 tACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTg                                                                                               >  2:89043/1‑149 (MQ=255)

                                                  aCATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGa                                                                                              <  2:184233/149‑1 (MQ=255)

                                                  aCATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGa                                                                                              <  2:312418/149‑1 (MQ=255)

                                                   cATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAg                                                                                             <  1:254746/149‑1 (MQ=255)

                                                    aTTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAgg                                                                                            <  1:1095559/149‑1 (MQ=255)

                                                    aTTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAgg                                                                                            >  1:496831/1‑149 (MQ=255)

                                                    aTTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAgg                                                                                            >  2:679856/1‑149 (MQ=255)

                                                    aTTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAgg                                                                                            <  1:375869/149‑1 (MQ=255)

                                                    aTTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTACCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAgg                                                                                            <  2:739467/149‑1 (MQ=255)

                                                     ttGCTGTGTCGGGTAAGCCATTACGATATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGt                                                                                           <  1:1158414/149‑1 (MQ=255)

                                                         tgtgTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGAt                                                                                       <  2:341131/149‑1 (MQ=255)

                                                          gtgtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGC‑TTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGAtgt                                                                                     <  2:1169642/149‑1 (MQ=255)

                                                           tgtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGAtgt                                                                                     >  1:1066415/1‑149 (MQ=255)

                                                           tgtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGAtgt                                                                                     >  1:692947/1‑149 (MQ=255)

                                                             tCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCTTCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGc                                                                                   >  1:746009/1‑149 (MQ=255)

                                                               gggTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAg                                                                                 <  2:558666/149‑1 (MQ=255)

                                                               gggTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTGTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TGTTTTCCCGAGTTCCCTTTTGAGGTTGATGTGCAg                                                                                 >  1:215082/1‑149 (MQ=255)

                                                                ggTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGTAGt                                                                                >  1:1157202/1‑149 (MQ=255)

                                                                 gTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGtt                                                                               >  2:345306/1‑149 (MQ=255)

                                                                    aGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTaa                                                                             <  1:786203/149‑1 (MQ=255)

                                                                      ccATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGGTGTGCAGTTAACGa                                                                          >  2:647020/1‑149 (MQ=255)

                                                                      ccATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTACCTTTTGAGGTTGATGTGCAGTTAACGa                                                                          >  2:574554/1‑149 (MQ=255)

                                                                      ccAATACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCGCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGa                                                                          <  2:849963/149‑1 (MQ=255)

                                                                              cTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACt                                                                  <  2:1079565/149‑1 (MQ=255)

                                                                               tATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTg                                                                 >  1:912274/1‑149 (MQ=255)

                                                                                    gACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGGGTTTCACTgattac                                                            >  1:132559/1‑149 (MQ=255)

                                                                                    gACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattac                                                            >  1:886109/1‑149 (MQ=255)

                                                                                     acacAGTGTTATATCGTCGCTTTTTTCCTTCCCCGAACTG‑AAATATATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacg                                                           <  1:197942/149‑1 (MQ=255)

                                                                                         aGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggatt                                                       >  2:30561/1‑149 (MQ=255)

                                                                                         aGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggatt                                                       <  2:1039912/149‑1 (MQ=255)

                                                                                             ttAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGt                                                   >  1:639300/1‑149 (MQ=255)

                                                                                             ttAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGt                                                   >  1:849960/1‑149 (MQ=255)

                                                                                             ttAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGt                                                   >  1:1076811/1‑149 (MQ=255)

                                                                                             ttAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGt                                                   >  2:1169234/1‑149 (MQ=255)

                                                                                              tAAATCCTCGCTTTTTTCCTTCCCCGAACTT‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGGTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTg                                                  <  1:743954/149‑1 (MQ=255)

                                                                                              tAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTg                                                  <  2:1066415/149‑1 (MQ=255)

                                                                                                 aTCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTgcgc                                               >  2:68723/1‑149 (MQ=255)

                                                                                                 aTCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTgcgc                                               >  2:440499/1‑149 (MQ=255)

                                                                                                     tCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGAt                                           >  1:754977/1‑149 (MQ=255)

                                                                                                     tCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGAt                                           >  2:759267/1‑149 (MQ=255)

                                                                                                     tCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGAt                                           <  2:492683/149‑1 (MQ=255)

                                                                                                     tCGCGTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCCGTGATTACGGATTACGTGCGCTGAt                                           >  2:938516/1‑149 (MQ=255)

                                                                                                           ttttCCTTCCCCGAAATG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACAt                                     <  2:229637/149‑1 (MQ=255)

                                                                                                             ttccttccCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATgg                                   <  2:774293/149‑1 (MQ=255)

                                                                                                             ttcattccCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGTTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATgg                                   <  2:704481/149‑1 (MQ=255)

                                                                                                              tccttccCCGAACTT‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGc                                  <  1:303666/149‑1 (MQ=255)

                                                                                                                cttccCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTACCGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGt                                <  2:1121376/149‑1 (MQ=255)

                                                                                                                 ttccCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTc                               >  2:1168827/1‑149 (MQ=255)

                                                                                                                  tccCCGAACTG‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCa                              <  1:867871/149‑1 (MQ=255)

                                                                                                                            g‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGaa                     <  2:336130/149‑1 (MQ=255)

                                                                                                                            g‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTTCCCGATTTCCCTTTTGAGGTTGAGGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGaa                     <  2:860256/149‑1 (MQ=255)

                                                                                                                            g‑AAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAg                    >  2:829336/1‑149 (MQ=255)

                                                                                                                              aaataaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAgg                   >  1:933723/1‑149 (MQ=255)

                                                                                                                              aaataaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAgg                   >  2:1070467/1‑149 (MQ=255)

                                                                                                                                ataaatTAGCGACACAGCCGGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGc                 <  1:768824/149‑1 (MQ=255)

                                                                                                                                 taaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCg                <  1:556592/149‑1 (MQ=255)

                                                                                                                                 taaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCg                <  1:383679/149‑1 (MQ=255)

                                                                                                                                   aatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGa              <  1:2593/149‑1 (MQ=255)

                                                                                                                                   aatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGa              >  1:237892/1‑149 (MQ=255)

                                                                                                                                   aatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGa              <  2:1114521/149‑1 (MQ=255)

                                                                                                                                    atTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGAt             <  1:836568/149‑1 (MQ=255)

                                                                                                                                    atTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGAt             >  2:1002508/1‑149 (MQ=255)

                                                                                                                                       aGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGAc          <  1:525934/149‑1 (MQ=255)

                                                                                                                                       aGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGAc          <  2:886109/149‑1 (MQ=255)

                                                                                                                                         cGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCa        <  1:317373/149‑1 (MQ=255)

                                                                                                                                         cGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACCGATTACGTGCGCTGATCTACATGGCTTCATTGCCAGAAGGGCGGGTGACCa        <  1:482649/149‑1 (MQ=255)

                                                                                                                                          gACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAg       <  2:282355/149‑1 (MQ=255)

                                                                                                                                           acacAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGt      >  2:1021391/1‑149 (MQ=255)

                                                                                                                                           acacAGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGt      >  1:859083/1‑149 (MQ=255)

                                                                                                                                            cactGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACTAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTa     <  1:187841/149‑1 (MQ=255)

                                                                                                                                             acaGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTAt    >  2:303897/1‑149 (MQ=255)

                                                                                                                                              caGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTAtt   >  2:701534/1‑149 (MQ=255)

                                                                                                                                               aGCCTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTAttt  <  1:460960/149‑1 (MQ=255)

AATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTG‑AAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTA‑TTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTT  >  NC_000913/4405984‑4406274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`