| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 287 | Δ1 bp | 5.4% | intergenic (+32/‑50) | thrL → / → thrA | thr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 287 | 0 | C | . | 5.4% | 1103.0 / 56.9 | 312 | intergenic (+32/‑50) | thrL/thrA | thr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1 |
| Reads supporting (aligned to +/- strand): ref base C (149/143); new base . (11/6); total (161/151) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 318 total aligned reads displayed.
CAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGG > NC_000913/138‑436 | aaTATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCAcc > 1:1798391/1‑150 (MQ=255) taGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGAc > 1:579041/1‑150 (MQ=255) aGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACa > 1:1097979/1‑150 (MQ=255) aTAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGc < 2:2873724/150‑1 (MQ=255) tAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCg > 1:3689787/1‑150 (MQ=255) tAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCg < 1:3587494/150‑1 (MQ=255) gcgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCggg < 2:2488191/150‑1 (MQ=255) cgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCA‑CTGTCAGGTGCGGGc < 2:587198/150‑1 (MQ=255) acagacagATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCatt > 2:3335059/1‑150 (MQ=255) agacagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCttttt > 1:1438545/1‑150 (MQ=255) agacagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCttttt > 1:325944/1‑150 (MQ=255) cagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCtttttttt < 2:4147413/150‑1 (MQ=255) agaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCttttttttt < 1:30872/150‑1 (MQ=255) aTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCg > 2:1051503/1‑150 (MQ=255) tAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCCCCACCATCCCCATTACCACCGGTAACGGTGCGGGCTGACGCGTAAAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGa < 1:1031582/150‑1 (MQ=255) tAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGa > 2:1940330/1‑150 (MQ=255) aaaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCTTACAGGAAACACAGAAAAAATCCCGCACCTGACA‑GTGCTGGCTTTTGTTTTGGAcaa > 2:2942315/1‑148 (MQ=255) aaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCaa < 1:1984383/150‑1 (MQ=255) gagTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCCAAGGTAAc > 2:2006148/1‑150 (MQ=255) tACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAg < 1:2681691/150‑1 (MQ=255) acaacaTCAATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTa < 1:1845767/150‑1 (MQ=255) caacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTCCCACAGGTAACGGGGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GGGCGGGCTTTTTTTTTCGCCCCACGGTCACGACGTaa > 2:6022654/1‑150 (MQ=255) aacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTaac < 1:223290/150‑1 (MQ=255) aacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTaac < 1:5571847/150‑1 (MQ=255) aacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTaac < 1:5952975/150‑1 (MQ=255) aTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTc < 1:497261/126‑1 (MQ=255) aTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTc > 2:497261/1‑126 (MQ=255) tCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAATGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGTCCAAAGGTAACGAGGTAACAAcc > 1:3861306/1‑150 (MQ=255) aTGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATg > 1:4950408/1‑150 (MQ=255) tGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAATGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGc > 2:3946068/1‑150 (MQ=255) tGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGc < 1:334671/150‑1 (MQ=255) aaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAg > 2:1700363/1‑150 (MQ=255) aaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAg > 2:4371035/1‑150 (MQ=255) aCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAgt < 2:6433495/150‑1 (MQ=255) cGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAgtg < 2:5298431/150‑1 (MQ=255) aTTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTg > 2:5578254/1‑150 (MQ=255) ttAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGa < 1:5318341/150‑1 (MQ=255) tcgCACCACCAGTCCCACCACCATCACCATTACCACCGGTACCGGTGCGGGCGGCCGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACT‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa < 1:4416654/148‑1 (MQ=255) tAGCACCACCTTTACCACCCCCATCACCATTACCACAGTTAACGGTGCGGGCTGACGGGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa < 2:3499747/150‑1 (MQ=255) tAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa < 2:2085677/150‑1 (MQ=255) tAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa < 1:675644/150‑1 (MQ=255) tAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCAGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCATTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa < 1:6256126/150‑1 (MQ=255) caccacCATTACCACCCCCATCACCATTACCACTGGTACCGGTGCGGGCTGACGCGTACAGTAAACACCGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGtt < 1:288016/150‑1 (MQ=255) caccacCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGtt < 1:4507732/150‑1 (MQ=255) caccaTTACCACCACCCTCACCATTACCACCGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcgg < 2:5933766/150‑1 (MQ=255) ccaccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAGACACAGAAAAAACCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACAt < 2:3622518/150‑1 (MQ=255) caccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa > 2:507305/1‑135 (MQ=255) caccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa < 1:507305/135‑1 (MQ=255) caccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGGTCGGCGGTACATc > 2:4882522/1‑150 (MQ=255) accaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCa < 2:4279013/150‑1 (MQ=255) accaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCa < 2:3689787/150‑1 (MQ=255) ccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGACGTTCGGCGGTACATCAg > 2:3793177/1‑150 (MQ=255) ccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAg > 1:3120580/1‑150 (MQ=255) accctcaccatTACCACCGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTg < 1:3177982/150‑1 (MQ=255) accatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTg < 2:3294512/150‑1 (MQ=255) ccatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTgg < 1:6035251/150‑1 (MQ=255) atcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCa > 1:6547875/1‑150 (MQ=255) ccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTACCGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATg > 2:1662194/1‑150 (MQ=255) atTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCa > 2:2739177/1‑150 (MQ=255) aCCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGaa < 1:2547548/150‑1 (MQ=255) aCCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGaa > 2:2518410/1‑150 (MQ=255) cacaGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACg > 1:4648095/1‑150 (MQ=255) acaGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGt > 2:4664048/1‑150 (MQ=255) acaGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGt > 1:1242870/1‑150 (MQ=255) gTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCaa < 2:714032/79‑1 (MQ=255) cGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCg > 2:3080258/1‑150 (MQ=255) tGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCgtgt > 2:532732/1‑150 (MQ=255) gggCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGc < 1:5160014/150‑1 (MQ=255) ggCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGcc > 2:2961077/1‑150 (MQ=255) gCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCg < 1:1956459/150‑1 (MQ=255) cTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGa < 1:952410/150‑1 (MQ=255) tGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGat > 2:3389961/1‑150 (MQ=255) gACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGata < 1:2083249/150‑1 (MQ=255) aCGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTAGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTAGGCGGGACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGatat > 1:1542125/1‑150 (MQ=255) gcgTACAGGAAACACAGAAAAAAGCCCGCCCCTGACA‑GTGCGGGCTTTTTTTTTCGACCCAAGGTACCGCGGGAACCACCATGCGAGGGTTGAAGTTCGGCGGGACCACAGGGGCAAATGCCGGCCGGTTTCGGCGTGTTGCCGaacgtc > 1:5256998/1‑145 (MQ=255) gcgTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTc > 2:153821/1‑150 (MQ=255) gTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGttt > 2:2198839/1‑128 (MQ=255) gTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTg < 1:1378160/150‑1 (MQ=255) gTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTg < 1:2961077/150‑1 (MQ=255) cAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGaa < 2:1591453/150‑1 (MQ=255) cAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGaa < 1:1900059/150‑1 (MQ=255) aGGAAACACAGAAAAATGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGTAGTTCGGCGGTACAGCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGaaa > 2:6203349/1‑150 (MQ=255) gaaaaaaaaaGCCCGCA‑CTGTCAGGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGaa > 2:3157145/5‑142 (MQ=38) acaGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGc > 2:6556197/1‑150 (MQ=255) acaGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGc < 1:6513189/150‑1 (MQ=255) acaGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGc > 2:55395/1‑150 (MQ=255) aGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCca < 2:1242870/150‑1 (MQ=255) aaaaaaGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcagg > 2:1730430/1‑150 (MQ=255) aaaaaGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggc > 2:3251489/1‑150 (MQ=255) aaaaGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggca < 2:54061/150‑1 (MQ=255) aaGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggcagg < 2:1480886/150‑1 (MQ=255) aaGCCCGCA‑CTGTCAGGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTGCATCAGTGGCAAATGCAGAACGTTTTATGCGGGTTGCCGATATTCTGGAAAGCAATGCcaggcagg > 1:5436928/1‑150 (MQ=255) aaGCCCGCA‑CTGTCAGGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAAACAAGCGAGTGTTGAAGTTAGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggcagg > 1:3966967/1‑150 (MQ=255) aGCCCGCA‑CTGTCAGGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCTGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggg > 2:86178/1‑150 (MQ=255) gCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcagggg > 2:2385618/1‑150 (MQ=255) cccGCAACTGACA‑GTGCGGGCTTTTTTTTTAGACCAAATGTAACGAGGTAACAACCAAGCGACTGTTGGAGTTCGGCGGTACATAAGTGGCAAATGCAGAAAGATTTCTGCGTTTTGCCGATATTCTGGAAAGCAAGGCCAggccggggc > 1:4247909/1‑150 (MQ=255) ccGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGGGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggca > 2:1865359/1‑150 (MQ=255) ccGCACCTGACA‑GTGCGGGCTTTTTTTTTAGAACAAAGGTAACGAGGTAACAACCAAGCGAGTGTTGAAGTTCTGCGGTACATCAGTTGCAAAATCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAATCAATTCCAggcaggggca > 1:2800960/1‑150 (MQ=255) cGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTAGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggcag > 1:882914/1‑150 (MQ=255) ccTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTgg < 2:268318/150‑1 (MQ=255) | CAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGG > NC_000913/138‑436 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |