| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 293:1 | +G | 5.6% | intergenic (+38/‑44) | thrL → / → thrA | thr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 293 | 1 | . | G | 5.6% | 1406.8 / 74.9 | 305 | intergenic (+38/‑44) | thrL/thrA | thr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1 |
| Reads supporting (aligned to +/- strand): ref base . (142/146); new base G (11/6); total (153/152) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.97e-01 | |||||||||||
Only 100 of 312 total aligned reads displayed.
AGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG > NC_000913/144‑442 | aGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACa > 1:1097979/1‑150 (MQ=255) ggCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑g > 1:5270650/1‑150 (MQ=255) cATAGCGCACAGAAATATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACTGTTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTg < 1:3756054/150‑1 (MQ=255) aTAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGc < 2:2873703/150‑1 (MQ=255) aTAGCGCACAGAAATATAAAAATTCCAGATTACACAACATCCATGAAACGCATTCGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGc < 1:3301309/150‑1 (MQ=255) tAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCg > 2:1317941/1‑150 (MQ=255) gcgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCggg < 2:2488191/150‑1 (MQ=255) cgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGc < 2:5989434/150‑1 (MQ=255) gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑t > 1:3496774/1‑150 (MQ=255) gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑t > 2:3071901/1‑150 (MQ=255) acagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑ttttttt > 1:4984953/1‑150 (MQ=255) agaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACCCAT‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑ttttttttt < 2:3123552/150‑1 (MQ=255) tAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCCCCACCATCCCCATTACCACCGGTAACGGTGCGGGCTGACGCGTAAAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGa < 1:1031582/150‑1 (MQ=255) tAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGa > 2:334671/1‑150 (MQ=255) cacaACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACTGGAAACACAG‑AAATTTGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGTCCAATGGTAACGAGGt > 1:3182592/1‑150 (MQ=255) acaacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTa > 2:1095487/1‑150 (MQ=255) aacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTaac < 1:5571987/150‑1 (MQ=255) aacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTaac > 2:1904828/1‑150 (MQ=255) caTCCATGAATCGCATTAGCACCACCATTACCACCACAATCACCATTACCACAGTTATCGGTGCGGGCTGACGCGTACATTAAAAACAG‑AAAAAAGCCCGCACCTGACA‑TTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTaacaa < 1:4748322/150‑1 (MQ=255) caTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTaacaa < 1:1426167/150‑1 (MQ=255) caTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTaacaa < 1:442134/150‑1 (MQ=255) cATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCAt > 1:1478216/1‑150 (MQ=255) aTGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATg > 1:268318/1‑150 (MQ=255) aTGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATg > 1:1864407/1‑150 (MQ=255) tGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGc < 2:2687219/150‑1 (MQ=255) aaaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGAACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGa < 1:711836/150‑1 (MQ=255) aaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAg > 2:1700363/1‑150 (MQ=255) aaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAg > 2:4371129/1‑150 (MQ=255) aCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAgt < 2:6433495/150‑1 (MQ=255) aTTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAATGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTg > 2:1998285/1‑150 (MQ=255) aTTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTg > 2:4190690/1‑150 (MQ=255) aTTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTg > 2:5578254/1‑150 (MQ=255) ttAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGa < 1:5321388/150‑1 (MQ=255) tAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa < 2:2085677/150‑1 (MQ=255) tAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa < 2:5346533/150‑1 (MQ=255) ccaccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcg > 1:183115/1‑150 (MQ=255) caccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTCACAACCATGCGAGTGTTGAAGGTcgg > 2:4676801/1‑150 (MQ=255) accaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggc < 1:6687343/150‑1 (MQ=255) ttACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTa < 1:3613210/150‑1 (MQ=255) atcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCa > 2:3758342/1‑150 (MQ=255) atcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGGGTTGAAGTTCGGCGGTACATCAGTGGcc > 1:6547831/1‑149 (MQ=255) tcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTTCGCCCCAAGGTAACGAGGTAACAACCCTGCGGGGGTTGAAGTTCGGCGGTACAACAGTGGCa > 1:3049377/1‑150 (MQ=255) ccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTACCGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATg > 2:1662194/1‑150 (MQ=255) catTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGc > 2:3614339/1‑150 (MQ=255) atTACCACAGGTACCGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCCCCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGGACAGCCGTGGCAAATGCa > 1:4529842/1‑150 (MQ=255) atTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCa < 1:549435/150‑1 (MQ=255) atTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCa < 2:3276340/150‑1 (MQ=255) ttACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAAGCCCGCACATGACA‑TTGCGGGG‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACAATGCGAGTTTTGAGGTGCGGCGGTCCAGCAGTGGCAAATGCa > 2:3683978/1‑150 (MQ=255) ttACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAg < 1:582328/150‑1 (MQ=255) aCCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGaa > 2:2518410/1‑150 (MQ=255) ccACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTTCCACCAACGGTAACGAGGTACCAACCATCCGAGGGTTGAAGTTCGGCGGTACATCATTGGCAATTGCAGaa > 2:3988615/1‑150 (MQ=255) acaGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTg > 1:6398138/1‑114 (MQ=255) acaGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGt > 1:1242870/1‑150 (MQ=255) gTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCTGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTc > 1:1934238/1‑150 (MQ=255) gTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCaa < 2:714032/79‑1 (MQ=255) cGGTGCGGGCTGACGCGTACAGGAAACACAC‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGTACGTTTTCTGCg < 1:6180227/150‑1 (MQ=255) ggTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCgt < 1:2479478/150‑1 (MQ=255) tGCGGGCTGACGCGGCCAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCgtgt < 2:3413163/150‑1 (MQ=255) ggCGGGCTGTCGCGTACTGGAAACACAC‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGTGGTAACAACCATGCGAGTGTTTAAGTTCGGCGGTACATCAGTGGCAAATGCAGATCGTTTTCTGCgtgt < 2:5134568/149‑1 (MQ=255) ggCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGcc > 2:2960851/1‑150 (MQ=255) gCTGACGCGTACAGGAAACACAG‑AATACAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCACAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCg < 2:4283055/150‑1 (MQ=255) aCGCGTACAGGAAACACAG‑AAAAATGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGTCCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTAGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGatat > 1:1542381/1‑150 (MQ=255) gcgTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTc > 2:153821/1‑150 (MQ=255) cgTACAGGAAACACAG‑AAAAAAGCCCGCACCTTACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAAAAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCTAATTCAGAACGTTTTGTGCGTGTTTGCGATATTCt > 1:2654417/1‑150 (MQ=255) cgTACAGCAAACACAG‑AACAATGCCCCCAGCTGACG‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCt < 1:2942315/150‑1 (MQ=255) gTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGttt > 2:2198839/1‑128 (MQ=255) gTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGttt < 1:2198288/128‑1 (MQ=255) gTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTg < 1:3946068/150‑1 (MQ=255) gTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTg < 1:1982566/150‑1 (MQ=255) aCAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGa < 1:5978681/150‑1 (MQ=255) cAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGaa < 2:1591453/150‑1 (MQ=255) aGGAAACACAG‑AAAAATGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGTAGTTCGGCGGTACAGCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGaaa > 2:6203349/1‑150 (MQ=255) ggAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGGTTTCTGCGTGTTGCCGATATTCTGGAAAg > 2:1019834/1‑150 (MQ=255) aaCACCG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCaa < 2:4950408/150‑1 (MQ=255) acacAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAAt > 2:530480/1‑150 (MQ=255) acaG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGc < 1:2063963/150‑1 (MQ=255) acaG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGc < 1:4340458/150‑1 (MQ=255) acaG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGc > 2:6556197/1‑150 (MQ=255) acaG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGc > 2:55395/1‑150 (MQ=255) gaaaaaaaaaGCCCGCA‑CTGTCAGGTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGaa < 1:3157145/138‑1 (MQ=38) aG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCca > 2:5873992/1‑150 (MQ=255) aG‑AAAAAAGCCCGAACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCca < 1:495498/150‑1 (MQ=255) aaaaaaGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcagg > 2:1730430/1‑150 (MQ=255) aaaaGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggca < 2:54061/150‑1 (MQ=255) aaaGCCCGCA‑CTGTCAGGTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggcag > 2:6040029/1‑150 (MQ=255) aaGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggcagg < 2:1480886/150‑1 (MQ=255) aaGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggcagg < 1:5250861/150‑1 (MQ=255) aaGCCCGCA‑CTGTCAGGTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggcagg > 2:3302720/1‑150 (MQ=255) aGCCCGCA‑CTGTCAGGTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCTGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggg > 2:86178/1‑150 (MQ=255) gCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcagggg > 2:2385618/1‑150 (MQ=255) gCCCGCA‑CTGTCAGGTGCGGGCTTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggg > 2:3238587/1‑150 (MQ=255) ccGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGTAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTATGGAAAGCAATGCCAggcaggggca > 1:2801094/1‑150 (MQ=255) ccGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggca > 2:1865063/1‑150 (MQ=255) ccGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTAAATCAGTGGCAAATGCAGAACGTTTTATGCGTGTTTCCGATATTCTGGAAAGCAATGCCAggcaggggca > 1:5102858/1‑150 (MQ=255) cGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggccg > 1:883077/1‑148 (MQ=255) cGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGACGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACCTTATCTGCGTGTTGCCGATATTATGGAAAGCAATGCCAggcaggggcag > 2:4995184/1‑150 (MQ=255) cACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGt < 1:3090262/150‑1 (MQ=255) aCCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTg > 2:3911697/1‑150 (MQ=255) aCCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTg < 2:2931537/150‑1 (MQ=255) aCCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTg < 1:4217544/150‑1 (MQ=255) | AGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAG‑AAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCACCG > NC_000913/144‑442 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |