Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,446,357:1 +T 6.5% intergenic (+151/+21) ydbL → / ← feaR DUF1318 family protein/transcriptional activator for tynA and feaB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,446,3571.T 6.5% 1136.0 / 70.4 263intergenic (+151/+21)ydbL/feaRDUF1318 family protein/transcriptional activator for tynA and feaB
Reads supporting (aligned to +/- strand):  ref base . (113/133);  new base T (13/4);  total (126/137)
Fisher's exact test for biased strand distribution p-value = 2.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01

Only 100 of 277 total aligned reads displayed.

AGCAAAACCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTTCATCATCTGCGGC  >  NC_000913/1446208‑1446506
                                                                                                                                                      |                                                                                                                                                     
aGCAAAACCGCAATCTCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCa                                                                                                                                                        <  1:5824115/150‑1 (MQ=255)
aGCAAAACCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCa                                                                                                                                                        <  2:367126/150‑1 (MQ=255)
aGCAAAACCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCa                                                                                                                                                        <  2:3219248/150‑1 (MQ=255)
 gCAAAACCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAg                                                                                                                                                      >  1:1089413/1‑150 (MQ=255)
  cAAAACCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGt                                                                                                                                                     >  2:804916/1‑150 (MQ=255)
  cAAAACCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGt                                                                                                                                                     >  2:1817859/1‑150 (MQ=255)
  cAAAACCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGt                                                                                                                                                     >  1:2528215/1‑150 (MQ=255)
   aaaaCCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTa                                                                                                                                                    <  2:3194258/150‑1 (MQ=255)
   aaaaCCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTa                                                                                                                                                    >  1:678726/1‑150 (MQ=255)
       ccGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATg                                                                                                                                                <  2:3096037/150‑1 (MQ=255)
       ccGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATg                                                                                                                                                >  2:4105430/1‑150 (MQ=255)
       ccGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATg                                                                                                                                                <  1:4183879/150‑1 (MQ=255)
       ccGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATg                                                                                                                                                <  1:3762361/150‑1 (MQ=255)
        cGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGc                                                                                                                                               <  2:2320972/150‑1 (MQ=255)
        cGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGc                                                                                                                                               >  2:1272770/1‑150 (MQ=255)
             gatCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGc                                                                                                                                          <  2:2170109/149‑1 (MQ=255)
                 gggATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAt                                                                                                                                      >  2:676437/1‑150 (MQ=255)
                      aaCGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttagtt                                                                                                                                 >  2:5919062/1‑150 (MQ=255)
                        cGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttagttag                                                                                                                               >  1:4787493/1‑150 (MQ=255)
                             cAGGAGAAGGCGATTGATCTATGTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGaa                                                                                                                          <  2:5274200/150‑1 (MQ=255)
                              aGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAAt                                                                                                                         >  1:4734424/1‑150 (MQ=255)
                                      gCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCg                                                                                                                 >  2:5630023/1‑150 (MQ=255)
                                         aTTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATa                                                                                                              >  2:2461246/1‑150 (MQ=255)
                                              tCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGc                                                                                                         >  1:2777927/1‑150 (MQ=255)
                                              tCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGc                                                                                                         >  1:3555093/1‑150 (MQ=255)
                                                tATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCt                                                                                                       <  2:5427510/150‑1 (MQ=255)
                                                tATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCt                                                                                                       <  2:264224/150‑1 (MQ=255)
                                                tATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCt                                                                                                       >  1:2377180/1‑150 (MQ=255)
                                                tATTTTCATTAAAAAAGTTTAATATTCAAAAACTCCTGTCAAATTTAATTTTGCCATGAAAAATTCATATTAAAATATCTTTTTTAACTAATTGTATTTTCAGTAAATTCAAAGCAAATTAGTTAGCGGAATTTAAGTCTATAATCgaat                                                                                                       <  2:4518643/150‑4 (MQ=255)
                                                  ttttCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGACTTTACGTCGATACTCGCCTgg                                                                                                     >  1:5066268/1‑150 (MQ=255)
                                                  cttgccTGAAACAAGGAGAATAGTCAAAAACTCCTGTCAACTTGCCTGTTGCCCTGAAAAATGCATAGTCAAAGCTCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTgt                                                                                                     <  2:5810040/146‑2 (MQ=255)
                                                   tttCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGc                                                                                                    <  1:5986052/150‑1 (MQ=255)
                                                   tttCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTAGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTAGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTgtc                                                                                                    <  2:1004576/150‑3 (MQ=255)
                                                       cTGAACAAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAATATTCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCATAGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCa                                                                                                <  1:3652341/150‑1 (MQ=255)
                                                       cTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCa                                                                                                <  2:1761102/150‑1 (MQ=255)
                                                        tGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCAt                                                                                               <  1:811125/150‑1 (MQ=255)
                                                           aaCAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAAATTACGTCGATACTCGCCTGGCGTCATccc                                                                                            >  2:4352280/1‑150 (MQ=255)
                                                                 gTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCg                                                                                      >  1:5664851/1‑150 (MQ=255)
                                                                   gAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGtt                                                                                    <  1:3189580/150‑1 (MQ=255)
                                                                   gAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGtt                                                                                    <  1:1414648/150‑1 (MQ=255)
                                                                          cAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTaaa                                                                             <  1:1307035/150‑1 (MQ=255)
                                                                          cAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTCGCGTCATCCCAAAGCGTTGCTTaaa                                                                             >  1:1508523/1‑150 (MQ=255)
                                                                           aaaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAAc                                                                                                                                            <  2:6419069/86‑1 (MQ=38)
                                                                           aaaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAAc                                                                                                                                            >  1:6419069/1‑86 (MQ=38)
                                                                           aaaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAAt                                                                            >  1:1181766/1‑150 (MQ=255)
                                                                             aaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATAc                                                                          <  2:3555093/150‑1 (MQ=255)
                                                                              aaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATAcc                                                                         <  2:1508523/150‑1 (MQ=255)
                                                                                    tGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGTGTCATCCCAAAGCGTTGCTTAAATACCGTTGaa                                                                   >  1:4927483/1‑150 (MQ=255)
                                                                                    tGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaa                                                                   >  1:911856/1‑150 (MQ=255)
                                                                                    tGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaa                                                                   >  2:6535657/1‑150 (MQ=255)
                                                                                     gTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaa                                                                  >  1:719720/1‑150 (MQ=255)
                                                                                         aaTTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATg                                                              >  1:2988753/1‑150 (MQ=255)
                                                                                              ccTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGt                                                                                                                                                     >  1:5593716/1‑58 (MQ=255)
                                                                                               cTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTg                                                        <  2:5200270/150‑1 (MQ=255)
                                                                                                ttttGCCCTGAAAAATGCATAGTCAAATATCTGTGTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGGTGCTGCAATACCGTTGAAAAATGACTCTgg                                                       >  2:3707356/1‑150 (MQ=255)
                                                                                                 tttGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGt                                                      >  1:227137/1‑150 (MQ=255)
                                                                                                     cccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGa                                                  >  2:3370890/1‑150 (MQ=255)
                                                                                                        tGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGaaaa                                               >  2:3923227/1‑150 (MQ=255)
                                                                                                        tGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGaaaa                                               >  1:544701/1‑150 (MQ=255)
                                                                                                          aaaaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATc                                             <  1:3875150/150‑1 (MQ=255)
                                                                                                           aaaaTGCATAGTCAAATATCTGTTTTAACTAATGGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATcc                                            <  2:3759234/150‑1 (MQ=255)
                                                                                                            aaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATccc                                           <  1:441755/150‑1 (MQ=255)
                                                                                                            aaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATccc                                           <  1:9632/150‑1 (MQ=255)
                                                                                                            aaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATccc                                           <  1:4305966/150‑1 (MQ=255)
                                                                                                            aaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATccc                                           <  1:4231421/150‑1 (MQ=255)
                                                                                                                  aTAGTCAAAAATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGa                                     >  2:4479414/1‑150 (MQ=255)
                                                                                                                      tCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGc                                 <  2:1925069/150‑1 (MQ=255)
                                                                                                                      tCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGc                                 <  2:4659920/150‑1 (MQ=255)
                                                                                                                      tCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGc                                 >  1:4178967/1‑150 (MQ=255)
                                                                                                                        aaaTATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCg                               >  1:5163277/1‑150 (MQ=255)
                                                                                                                             tCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGAtgcc                          >  2:272624/1‑150 (MQ=255)
                                                                                                                              cTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGAtgcct                         <  2:5011862/150‑1 (MQ=255)
                                                                                                                              cTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGAtgcct                         <  1:4637877/150‑1 (MQ=255)
                                                                                                                               tGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGAtgcctg                        >  2:361819/1‑150 (MQ=255)
                                                                                                                                 ttttAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAAt                                                               >  2:2541987/1‑109 (MQ=255)
                                                                                                                                 ttttAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGACGAtgcctgcc                      >  1:867382/1‑150 (MQ=255)
                                                                                                                                  tttAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCa                     <  2:3634036/150‑1 (MQ=255)
                                                                                                                                    tAACTAATTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGt                   >  2:4901473/1‑150 (MQ=255)
                                                                                                                                    tAACTAATTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGt                   >  2:2499916/1‑150 (MQ=255)
                                                                                                                                      aCTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGttt                 >  2:2521861/1‑150 (MQ=255)
                                                                                                                                      aCTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATAACGTTGAAAAAAGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCCGttt                 >  1:281765/1‑150 (MQ=255)
                                                                                                                                        tAATTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCCGAAAATCCCCAATGAAAGCCGATGCCTGCCAGttttt               >  2:162066/1‑150 (MQ=255)
                                                                                                                                        tAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGttttt               <  2:2596887/150‑1 (MQ=255)
                                                                                                                                         aaTTTGAGTTTCACTAAATGCAACGCAAATTAGTTAGCGGAATTTACGTCGATACACTCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTCGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGATTTtc              <  1:658256/150‑1 (MQ=255)
                                                                                                                                         aaTTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGGTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGAGGCCTGCCAGTTTTtc              >  2:4768792/1‑150 (MQ=39)
                                                                                                                                         aaTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTtc              <  2:5207284/150‑1 (MQ=255)
                                                                                                                                         aaTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTtc              <  2:2057525/150‑1 (MQ=255)
                                                                                                                                         aaTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTtc              >  2:6365461/1‑150 (MQ=255)
                                                                                                                                         aaTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTtc              <  1:1903191/150‑1 (MQ=255)
                                                                                                                                           ttGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTtcat            >  2:6087486/1‑150 (MQ=255)
                                                                                                                                           ttGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTtcat            <  1:1007089/150‑1 (MQ=255)
                                                                                                                                            tGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTtcatc           >  2:2985357/1‑150 (MQ=255)
                                                                                                                                             ggCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTtcatca          <  2:5078223/150‑1 (MQ=255)
                                                                                                                                             ggCGTTGCAGTACATGCAACGCCAATTAGGTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTtcatca          >  2:344669/1‑150 (MQ=255)
                                                                                                                                              gCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTTcatcat         >  2:2635545/1‑150 (MQ=255)
                                                                                                                                              gCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTTcatcat         <  1:3368851/150‑1 (MQ=255)
                                                                                                                                                  tGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTTCATCATCTGc     >  2:5396347/1‑150 (MQ=255)
                                                                                                                                                   gCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTTCATCATCTGCg    >  1:210176/1‑150 (MQ=255)
                                                                                                                                                     aGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTTCATCATCTGCGGc  >  2:6190931/1‑150 (MQ=255)
                                                                                                                                                     aGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTTCATCATCTGCGGc  >  1:2294745/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
AGCAAAACCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTTCATCATCTGCGGC  >  NC_000913/1446208‑1446506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: