Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,792,766 A→T 5.9% intergenic (‑215/+60) kbl ← / ← yibB glycine C‑acetyltransferase/YibB family protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,792,7660AT 5.9% 1537.0 / 56.6 431intergenic (‑215/+60)kbl/yibBglycine C‑acetyltransferase/YibB family protein, function unknown
Reads supporting (aligned to +/- strand):  ref base A (193/211);  new base T (9/17);  total (203/228)
Fisher's exact test for biased strand distribution p-value = 2.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 445 total aligned reads displayed.

ACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCC  >  NC_000913/3792617‑3792915
                                                                                                                                                     |                                                                                                                                                     
aCGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCa                                                                                                                                                       >  2:2034602/1‑150 (MQ=255)
  gcgTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCaaa                                                                                                                                                     <  2:3794189/150‑1 (MQ=255)
  gcgTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCaaa                                                                                                                                                     >  1:5516369/1‑150 (MQ=255)
          acTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCAtt                                                                                                                                             >  2:1364186/1‑150 (MQ=255)
           cTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCAttt                                                                                                                                            <  1:1067099/150‑1 (MQ=255)
            ttCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCAtttt                                                                                                                                           <  2:78890/150‑1 (MQ=255)
            ttCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCAtttt                                                                                                                                           <  2:4690740/150‑1 (MQ=255)
            ttCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCAtttt                                                                                                                                           >  1:6230857/1‑150 (MQ=255)
            ttCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCAtttt                                                                                                                                           >  2:1077087/1‑150 (MQ=255)
              cTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTc                                                                                                                                         <  2:1911125/150‑1 (MQ=255)
              cTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTc                                                                                                                                         <  1:3159623/150‑1 (MQ=255)
               tGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTcc                                                                                                                                        >  1:57276/1‑150 (MQ=255)
                 cAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCATTTTTTCtt                                                                                                                                      <  2:5517622/150‑2 (MQ=255)
                 cAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTa                                                                                                                                      >  1:4488232/1‑150 (MQ=255)
                 cAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTa                                                                                                                                      >  1:2906890/1‑150 (MQ=255)
                         aGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCAtttt                                                                                                                                           <  2:1134507/137‑1 (MQ=255)
                         aGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGtt                                                                                                                              >  1:3411298/1‑150 (MQ=255)
                          ggATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGtta                                                                                                                             >  2:3940624/1‑149 (MQ=255)
                          ggATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTg                                                                                                                             >  1:4178630/1‑150 (MQ=255)
                            ataaataaCGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGTTATAAAAAGAAAAAATGATGCCAAAGCTTCTTTTTTCCTATCTCAGTTGGt                                                                                                                           >  1:2334767/1‑150 (MQ=255)
                              aaataaCGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTAt                                                                                                                         >  2:1661806/1‑150 (MQ=255)
                                  aaCGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCaaa                                                                                                                     >  2:4717680/1‑150 (MQ=255)
                                  aaCGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCaaa                                                                                                                     >  1:6130654/1‑150 (MQ=255)
                                   ccGTGTAACTTGATGTCTCTCAACACCTACTGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGTTTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCTAAGCATCATTTTTCCTATCTCAGTTGGTATCaaaa                                                                                                                    <  2:3482233/149‑1 (MQ=255)
                                    cGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAAc                                                                                                                   <  1:5578429/150‑1 (MQ=255)
                                        tAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTa                                                                                                               <  2:2830160/150‑1 (MQ=255)
                                          aCTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTAcc                                                                                                             <  2:4188960/150‑1 (MQ=255)
                                            ttGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCa                                                                                                           >  1:1350217/1‑150 (MQ=255)
                                              gATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACt                                                                                                         >  2:6112279/1‑150 (MQ=255)
                                                tGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTaa                                                                                                       <  2:5313618/150‑1 (MQ=255)
                                                  tctctcAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATg                                                                                                     >  2:6459251/1‑150 (MQ=255)
                                                    tctcAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATggc                                                                                                   >  2:2221887/1‑149 (MQ=255)
                                                     ctcAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAg                                                                                                  >  1:6297966/1‑150 (MQ=255)
                                                        aaCACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGa                                                                                               >  1:4092154/1‑150 (MQ=255)
                                                             cTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCt                                                                                          >  1:3726774/1‑150 (MQ=255)
                                                               aCAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTAt                                                                                        >  2:3091264/1‑150 (MQ=255)
                                                               aCAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTAt                                                                                        <  1:2173134/150‑1 (MQ=255)
                                                                 aGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTAttt                                                                                      >  1:14384/1‑150 (MQ=255)
                                                                  gATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTAtttt                                                                                     <  1:3147695/150‑1 (MQ=255)
                                                                   aTAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTc                                                                                    <  2:4049539/150‑1 (MQ=255)
                                                                    tagtagAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCt                                                                                   <  1:1867571/150‑1 (MQ=255)
                                                                     agtagAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCtt                                                                                  <  2:2749452/150‑1 (MQ=255)
                                                                     agtagAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCtt                                                                                  <  1:1988692/150‑1 (MQ=255)
                                                                     agtagAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCtt                                                                                  <  2:290180/150‑1 (MQ=255)
                                                                     agtagAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCtt                                                                                  <  2:4384938/150‑1 (MQ=255)
                                                                        agAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGaa                                                                               <  2:2506846/150‑1 (MQ=255)
                                                                        agAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGaa                                                                               <  1:3250405/150‑1 (MQ=255)
                                                                         gAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGaaa                                                                              <  1:2531311/150‑1 (MQ=255)
                                                                         gAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGaaa                                                                              <  1:3507736/150‑1 (MQ=255)
                                                                            aaaCTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGa                                                                           <  1:5179407/150‑1 (MQ=255)
                                                                             aaCTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGaa                                                                          <  1:4749306/150‑1 (MQ=255)
                                                                               cTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGaaaa                                                                        <  1:5892067/150‑1 (MQ=255)
                                                                                 gTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAAtt                                                                      <  2:3678812/150‑1 (MQ=255)
                                                                                     aGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCa                                                                  >  1:6438611/1‑150 (MQ=255)
                                                                                     aGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCa                                                                  <  2:6534023/150‑1 (MQ=255)
                                                                                       ttttGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAgc                                                                >  2:4494431/1‑150 (MQ=255)
                                                                                        tttGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAgcg                                                               <  2:1312188/150‑1 (MQ=255)
                                                                                              tGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCa                                                         >  1:6486119/1‑150 (MQ=255)
                                                                                                 gCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCAttt                                                      >  2:1020909/1‑150 (MQ=255)
                                                                                                     aaTGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGa                                                                           >  1:159852/1‑125 (MQ=255)
                                                                                                      atgatgTTTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGcc                                                 <  2:489464/150‑1 (MQ=255)
                                                                                                        gatgatTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCtt                                                                                  >  1:4924747/1‑115 (MQ=255)
                                                                                                        gatgatTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTGGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGcccc                                               >  2:5430833/1‑150 (MQ=255)
                                                                                                           gattgattTTACTGCATTGATTTAAAAAGAAAAAATGATGCCCAAGCATCATTTTTCCTATCTCAGGTGGTAGCAAAACGCTACCCACTACTGGAGGGAAACCTAGTTTCTTGACAGGCACATTCTCAGCGCCTGCATTGTTGcccggga                                            >  2:1530046/1‑146 (MQ=255)
                                                                                                            attgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTcaa                                           >  1:5849000/1‑150 (MQ=255)
                                                                                                            attgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTaaa                                           >  1:2101320/1‑150 (MQ=255)
                                                                                                             ttgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAgg                                                                                                 <  1:460640/95‑1 (MQ=255)
                                                                                                             ttgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAgg                                                                                                 >  2:460640/1‑95 (MQ=255)
                                                                                                             ttgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGACAATTCTCAGCGCCTGCATTTTTGCCCCTAAAg                                          >  2:5724461/1‑150 (MQ=255)
                                                                                                             ttgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAg                                          >  1:275480/1‑150 (MQ=255)
                                                                                                              tgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgt                                         <  2:2394460/150‑1 (MQ=255)
                                                                                                              tgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgt                                         <  2:664766/150‑1 (MQ=255)
                                                                                                                attTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtgt                                       >  1:3028769/1‑150 (MQ=255)
                                                                                                                  tttACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTa                                     <  2:1793816/150‑1 (MQ=255)
                                                                                                                  tttACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTa                                     <  1:507914/150‑1 (MQ=255)
                                                                                                                     aCTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTc                                  <  2:5880123/150‑1 (MQ=255)
                                                                                                                     aCTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTc                                  <  1:79961/150‑1 (MQ=255)
                                                                                                                       tGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCt                                <  1:1149838/150‑1 (MQ=255)
                                                                                                                       tGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCt                                <  1:3929507/150‑1 (MQ=255)
                                                                                                                         cATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGa                              <  2:4991697/150‑1 (MQ=255)
                                                                                                                           ttGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAag                            >  1:5270404/1‑150 (MQ=255)
                                                                                                                                ataAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGACGCAGCg                       >  2:6340278/1‑150 (MQ=255)
                                                                                                                                ataAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCg                       >  1:2416661/1‑150 (MQ=255)
                                                                                                                                 taAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGa                      >  1:169985/1‑150 (MQ=255)
                                                                                                                                 taAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGa                      <  1:3645299/150‑1 (MQ=255)
                                                                                                                                   aaaaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGaaa                    <  2:2597923/150‑1 (MQ=255)
                                                                                                                                   aaaaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGaaa                    <  2:1548777/150‑1 (MQ=255)
                                                                                                                                    aaaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAAc                   >  2:774606/1‑150 (MQ=255)
                                                                                                                                       gAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACaaa                <  1:224878/150‑1 (MQ=255)
                                                                                                                                        aaaaaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAAt               <  2:3161070/150‑1 (MQ=255)
                                                                                                                                        aaaaaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAAt               <  1:6679040/150‑1 (MQ=255)
                                                                                                                                         aaaaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGTAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATc              <  2:3334706/150‑1 (MQ=255)
                                                                                                                                         aaaaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATc              <  1:6048233/150‑1 (MQ=255)
                                                                                                                                         aaaaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATc              <  2:2123808/150‑1 (MQ=255)
                                                                                                                                          aaaaTGATGCTTTGGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCg             >  2:4741932/1‑150 (MQ=255)
                                                                                                                                            aaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGaa           <  1:897817/150‑1 (MQ=255)
                                                                                                                                               gatgCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCa        <  1:5430833/150‑1 (MQ=255)
                                                                                                                                                   ccAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCAtttt    >  1:3321207/1‑150 (MQ=255)
                                                                                                                                                    cAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTc   >  2:5755764/1‑150 (MQ=255)
                                                                                                                                                     aaaGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTcc  >  1:117822/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCC  >  NC_000913/3792617‑3792915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: