Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,244,593 T→G 7.5% E161D (GAA→GAC malF ← maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,244,5930TG 7.5% 1096.9 / 19.2 386E161D (GAA→GACmalFmaltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (151/198);  new base G (34/3);  total (185/201)
Fisher's exact test for biased strand distribution p-value = 3.97e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 429 total aligned reads displayed.

AGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGC  >  NC_000913/4244445‑4244741
                                                                                                                                                    |                                                                                                                                                    
 gagCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGtttc                                                                                                                                                    >  1:4178232/1‑150 (MQ=255)
 gagCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGttta                                                                                                                                                    >  1:796986/1‑149 (MQ=255)
   gCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGtttctt                                                                                                                                                  >  1:1653785/1‑150 (MQ=255)
    cGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGtttcttt                                                                                                                                                 >  2:5298068/1‑150 (MQ=255)
    cGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGtttcttt                                                                                                                                                 <  1:4548412/150‑1 (MQ=255)
    cGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGtttcttt                                                                                                                                                 <  1:3917116/150‑1 (MQ=255)
    cGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGtttcttt                                                                                                                                                 <  1:2533498/150‑1 (MQ=255)
     ggCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCGGGGTAATCACGCGCAGCTTCGCGCGGTCGCCGTCGGGCTGGGCGGGCGtgtcggtc                                                                                                                                                >  2:3139377/1‑146 (MQ=255)
        tGCGTGCCAGAACACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGt                                                                                                                                             <  2:3730887/150‑1 (MQ=255)
         gCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGtt                                                                                                                                            <  1:1121796/150‑1 (MQ=255)
         gCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGATCGTTTCTTTCAGtt                                                                                                                                            <  1:3539698/150‑1 (MQ=255)
           gTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGtttct                                                                                                                                                   <  2:4801192/141‑1 (MQ=255)
           gTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTATTTCAGTTGc                                                                                                                                          >  2:2507599/1‑150 (MQ=255)
            tGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCa                                                                                                                                         <  1:5798105/150‑1 (MQ=255)
            tGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCa                                                                                                                                         >  1:4448421/1‑150 (MQ=255)
            tGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCa                                                                                                                                         >  2:4517847/1‑150 (MQ=255)
            tGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTCGTTCAGTTgga                                                                                                                                         >  1:620888/1‑148 (MQ=255)
                aGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGttt                                                                                                                                     >  1:5751252/1‑150 (MQ=255)
                aGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGttt                                                                                                                                     <  2:2280747/150‑1 (MQ=255)
                 gAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTAAGTTGCAGtttt                                                                                                                                    >  2:2956941/1‑150 (MQ=255)
                         gcgcAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTTCTcgcc                                                                                                                            >  1:1554720/1‑150 (MQ=255)
                          cgcAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTGAGTTGGTGTTTTTGGTcgccg                                                                                                                           >  2:3402993/1‑150 (MQ=255)
                               ggAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCaaa                                                                                                                      <  1:553008/150‑1 (MQ=255)
                                gAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAAt                                                                                                                     >  2:4298593/1‑150 (MQ=255)
                                    tcatcatcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTATTTCAGTTTCAGTTTTTTCTCGCCGCCAAATTTaa                                                                                                                 >  1:1826805/1‑150 (MQ=255)
                                    tcatcatcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTCTTTCAGTTGGCGTTTTTGCTCGCCGCCACATTTaa                                                                                                                 >  1:2374575/1‑150 (MQ=255)
                                     catcatcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTaaa                                                                                                                <  1:3734210/150‑1 (MQ=255)
                                      atcatcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTAAGTTGCAGGTTTTGCTCGCCGCCCAAATTaaaa                                                                                                               >  1:2947916/1‑150 (MQ=255)
                                       tcatcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAg                                                                                                              >  2:2596543/1‑150 (MQ=255)
                                       tcatcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTCTTGCAGGTGCAGGTTTTGCTCGCCGCCAAATTTAAAAg                                                                                                              >  2:2040051/1‑150 (MQ=255)
                                        catcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTTCTAGCCGCCCAATTTAAAAGc                                                                                                             >  1:97715/1‑150 (MQ=255)
                                        catcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCCAATTTAAAAGc                                                                                                             >  1:6690278/1‑150 (MQ=255)
                                        catcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGc                                                                                                             <  1:759727/150‑1 (MQ=255)
                                        catcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGc                                                                                                             <  1:4021373/150‑1 (MQ=255)
                                          tcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTATTTAAGTTGCAGTTTTTTCTCGCCGCCCAATTTAAAATCGt                                                                                                           >  1:6450071/1‑150 (MQ=255)
                                           caCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTc                                                                                                          <  2:6230954/150‑1 (MQ=255)
                                            aCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCg                                                                                                         >  1:654227/1‑150 (MQ=255)
                                              tttGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTTCCGTTTTTGATCGCCGCCAAATTTAAAAGCGTCggg                                                                                                       >  1:1399933/1‑149 (MQ=255)
                                              tttGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTAGCCGCCAAATTTAAAAGCCTAGga                                                                                                       >  2:1217916/1‑150 (MQ=255)
                                                tgttgCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGgaga                                                                                                     <  1:5249944/150‑1 (MQ=255)
                                                 gttgCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGagag                                                                                                    <  2:5657025/150‑1 (MQ=255)
                                                 gttgCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGagag                                                                                                    <  1:318974/150‑1 (MQ=255)
                                                  ttgCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAgg                                                                                                   >  2:3847287/1‑150 (MQ=255)
                                                     ccATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTCTTTGAGTTTGAGTTTTTTCTCGCCGCCCAATTTAAAATCGTCGGAGAGGTaa                                                                                                >  1:4841917/1‑150 (MQ=255)
                                                      cATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAAt                                                                                               >  2:2219498/1‑150 (MQ=255)
                                                           ggCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTg                                                                                          <  1:5008883/150‑1 (MQ=255)
                                                            gCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGc                                                                                         <  2:6111695/150‑1 (MQ=255)
                                                                  tGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGGTTTTTCTCGCCGCCAAAAATAAAAGCGTGGGAGAGGTAATTTTTGGCGGgtt                                                                                   >  1:2180233/1‑150 (MQ=255)
                                                                        tAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGt                                                                             <  1:1158420/150‑1 (MQ=255)
                                                                         aaTGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTc                                                                            <  1:1461453/150‑1 (MQ=255)
                                                                         aaTGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTc                                                                            <  1:4779597/150‑1 (MQ=255)
                                                                          aTGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCg                                                                           <  1:5068670/150‑1 (MQ=255)
                                                                             tCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTGTTTGCTCGCCGCCCAAATTAAAAGCGTCGGAGAGGTAATTTTTTGCGGGTTGGcccgcgcgg                                                                        >  1:2643654/1‑143 (MQ=255)
                                                                              cACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGa                                                                       <  1:6134717/150‑1 (MQ=255)
                                                                              cACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTAGGAGAGGTAATTTTTTCCGGTTTAGCCGTCGCTGa                                                                       >  2:4002716/1‑150 (MQ=255)
                                                                                cTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAgc                                                                     <  2:5689398/150‑1 (MQ=255)
                                                                                 tCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTGAGTTTGAGTTTTTTCTAGCCGCCAAATTTAAAATAGTCGGAGAGGTAAATTTTTGCGGTTTCGCCGTCGCTGAgcg                                                                    >  2:5618337/1‑150 (MQ=255)
                                                                                 tCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCGTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCCGTTTAGCCCTCGCTGAgcg                                                                    >  2:5890731/1‑150 (MQ=255)
                                                                                   aGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGGTGCAGGTTTTGCTCGCCGCCCAATTTAAAAGCCGCGGCGAGGTAATTTTTGCCGGTTTCGCCCTCGCTGAGCGcc                                                                  >  2:4628489/1‑150 (MQ=255)
                                                                                          gACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTGTTTCAGGTGGAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGCGAGGTAATTTTTTCCGGTTTCGCCGTCGCGGAGCGCCAGTTGcc                                                           >  2:682362/1‑150 (MQ=255)
                                                                                          gACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGcc                                                           <  1:5298068/150‑1 (MQ=255)
                                                                                           aCGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCa                                                          <  1:1250226/150‑1 (MQ=255)
                                                                                           aCGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCa                                                          <  2:4192495/150‑1 (MQ=255)
                                                                                            cGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCAc                                                         <  1:3041397/150‑1 (MQ=255)
                                                                                             gATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTAGCCGTCGCTGAGCGCCAGTTGCCAca                                                        >  2:2811733/1‑149 (MQ=255)
                                                                                               ttCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCa                                                      <  1:5167331/150‑1 (MQ=255)
                                                                                                 cTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATc                                                    <  2:5043993/150‑1 (MQ=255)
                                                                                                  tGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTaa                                                                                                                 >  2:1577107/1‑88 (MQ=255)
                                                                                                  tGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTATTTGAGTTTCAGTTTTTGCTCGCCGCCCAAATTAAAAGACTACGAGAGGTAAATTTTGCCGGTTTAGCCGTCCCAGAGCGCCCGGTGCAAcacaaag                                                   >  2:6188689/1‑147 (MQ=255)
                                                                                                   gggTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGGTGCCACTCATcgc                                                  >  2:3294851/1‑150 (MQ=255)
                                                                                                         tCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCCGTTGCAGTTTTTGCTCGCCGCCCAATTTAAAAGCGTCGGAGAGGTACTTTTTGCCGGTTTAGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCccgccg                                            >  2:146880/1‑150 (MQ=255)
                                                                                                         tCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCccgccg                                            <  2:6446524/150‑1 (MQ=255)
                                                                                                          cACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCgg                                           <  2:4737096/150‑1 (MQ=255)
                                                                                                           aCGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCggg                                          <  1:2762636/150‑1 (MQ=255)
                                                                                                               gcAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTaaa                                      <  2:3677782/150‑1 (MQ=255)
                                                                                                                 aGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGa                                    <  2:1755043/150‑1 (MQ=255)
                                                                                                                      cgcgcgTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCTAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCaaa                               <  2:5793197/150‑1 (MQ=255)
                                                                                                                      cgcgcgTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCaaa                               <  1:1040892/150‑1 (MQ=255)
                                                                                                                      cgcgcgTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCaaa                               <  1:5575380/150‑1 (MQ=255)
                                                                                                                       gcgcgTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAg                              <  2:1856361/150‑1 (MQ=255)
                                                                                                                       gcgcgTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAg                              <  2:5198797/150‑1 (MQ=255)
                                                                                                                       gcgcgTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAg                              <  1:6328794/150‑1 (MQ=255)
                                                                                                                       gcgcgTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACACATCGCCCGCCCGGTAAAGACCAAAg                              >  2:1307468/1‑150 (MQ=255)
                                                                                                                        cgcgTTCGCCTTCGGGCTGGGCGGTCGTGTGGTTGCGTTTGAGGTTTTGCGCGCCGCCAAATTTAAAATCGTCGGGGAGGTAATTTTTGCCGGGTTAGCCGGCGCGGAGCGCCAGTTGCCACGCAACGCCCGCCGGGTAAAGACCAAAGt                             >  2:2284734/1‑150 (MQ=255)
                                                                                                                                ccTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGtt                     <  2:6590298/150‑1 (MQ=255)
                                                                                                                                 cTTCGGGCTGGGCGGTCGTTTCGTTCAGTTGCCGGTTTTGCTCGCCGCCCAATTTAAAAGCGTCGGAGAGGTAATTTTTTCCGGTTTAGCCCTCGCTGATCGCCAGTTGCCACGCAACGCCCCCCCGGTAAAGACCCAACTTATAGGttt                    >  2:44562/1‑150 (MQ=255)
                                                                                                                                   tCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTg                  <  2:350588/150‑1 (MQ=255)
                                                                                                                                    cGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTTCTCCCCCCCAAAATTAAAAGCGTAGGAGAGGTAATTTTTTCCGGTTTAGCCGTCGCTGAGCGCCAGTTTCCACTCAACGCCCCCCCGGTAAAGACCAAAGTTATAGGTTTTGc                 >  2:835104/1‑150 (MQ=255)
                                                                                                                                     gggCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGcc                >  2:6551750/1‑150 (MQ=255)
                                                                                                                                          gggCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAACAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCtt           <  2:4952367/150‑1 (MQ=255)
                                                                                                                                          gggCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCtt           <  1:1891310/150‑1 (MQ=255)
                                                                                                                                            gCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGc         <  2:17001/150‑1 (MQ=255)
                                                                                                                                            gCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGc         <  1:5407077/150‑1 (MQ=255)
                                                                                                                                             cGGTCGTTTCTTTCAGTTGCAGTTTTTGCTAGCCGCCAAATTTAAAAGCGTCGGAGAAGTAATTTTTGCCGGTTTAGCCCTCGCTGAGCGCCACTTTCCACAAATCGCCCGCCCGGTAAAGACCAAACGAATAGGTTTTTCCAGcattcc        >  2:6627415/1‑145 (MQ=255)
                                                                                                                                              ggTCGTGTCTTTCAGTTGCAGTTTGTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGGAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCa       <  2:4924363/150‑1 (MQ=255)
                                                                                                                                                 cGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGa    <  2:2751265/150‑1 (MQ=255)
                                                                                                                                                 cGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGa    <  1:3367860/150‑1 (MQ=255)
                                                                                                                                                  gTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGACGGTTTCGCCGACGATGAGAGCCAGTTGCCACTCATCTCCCGCCGGGAAAAGACAAAAGTAATAGGTTTTTCATGCTAGCCAGGAg   >  1:3438393/1‑150 (MQ=255)
                                                                                                                                                   tttctttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGc  <  1:2004035/150‑1 (MQ=255)
                                                                                                                                                   tttctttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGc  >  1:5991584/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGAGCGGCTGCGTGCCAGAAAACTGGCGCAGGGAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGC  >  NC_000913/4244445‑4244741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: