Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 418,088 C→G 5.2% P67R (CCG→CGG)  phoR → sensory histidine kinase in two‑component regulatory system with PhoB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913418,0880CG 5.2% 1536.1 / 26.5 439P67R (CCG→CGG) phoRsensory histidine kinase in two‑component regulatory system with PhoB
Reads supporting (aligned to +/- strand):  ref base C (239/172);  new base G (3/24);  total (242/197)
Fisher's exact test for biased strand distribution p-value = 1.21e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 443 total aligned reads displayed.

CTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAA  >  NC_000913/417939‑418236
                                                                                                                                                     |                                                                                                                                                    
 tgcCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCg                                                                                                                                                     >  2:597920/1‑150 (MQ=255)
 tgcCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCg                                                                                                                                                     >  2:3344653/1‑150 (MQ=255)
   ccTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCGTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCggg                                                                                                                                                   >  2:1942368/1‑150 (MQ=255)
      cccGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCg                                                                                                                                                >  1:1727060/1‑150 (MQ=255)
        cGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTg                                                                                                                                              >  1:5396075/1‑150 (MQ=255)
        cGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTg                                                                                                                                              >  1:2438886/1‑150 (MQ=255)
         ggCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTgg                                                                                                                                             >  2:3858737/1‑150 (MQ=255)
         ggCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTgg                                                                                                                                             >  1:5033120/1‑150 (MQ=255)
         ggCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACAGGGGAGTgg                                                                                                                                             <  2:1864131/150‑1 (MQ=255)
          gCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGt                                                                                                                                            <  1:883001/150‑1 (MQ=255)
               cATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTg                                                                                                                                       <  2:6693809/150‑1 (MQ=255)
                 tCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTggg                                                                                                                                     >  1:5490708/1‑150 (MQ=255)
                 tCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTggg                                                                                                                                     >  2:5035283/1‑150 (MQ=255)
                   cTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGaa                                                                                                                                   >  1:751673/1‑150 (MQ=255)
                    tGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAAc                                                                                                                                  >  2:2021125/1‑150 (MQ=255)
                    tGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAAc                                                                                                                                  >  2:4895692/1‑150 (MQ=255)
                      ggTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCg                                                                                                                                <  2:3635287/150‑1 (MQ=255)
                        tGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGct                                                                                                                              >  1:6536498/1‑150 (MQ=255)
                          cATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCCTGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGctac                                                                                                                            <  1:3240565/150‑1 (MQ=255)
                          cATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCAGTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGctac                                                                                                                            <  1:2649505/150‑1 (MQ=255)
                           aTTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGctact                                                                                                                           >  1:2576789/1‑150 (MQ=255)
                                 tGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACgg                                                                                                                     <  1:1432935/150‑1 (MQ=255)
                                 gggTGACCGGCCCTGGTTTGTGCTGGCATCGGTAACAGGACTGCTTATCGGGCATTTCTGGACTTGCTTGCGCCGTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACgg                                                                                                                     <  2:6492958/149‑1 (MQ=255)
                                        cTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTacac                                                                                                              <  2:2571378/150‑1 (MQ=255)
                                         tGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTAGTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACAcc                                                                                                             <  1:4269783/150‑1 (MQ=255)
                                          gCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCa                                                                                                            >  1:3445439/1‑150 (MQ=255)
                                          gCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCa                                                                                                            <  1:5959006/150‑1 (MQ=255)
                                          gCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTAGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCa                                                                                                            <  1:6168665/150‑1 (MQ=255)
                                            ccTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCGGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGa                                                                                                          <  1:3332170/150‑1 (MQ=255)
                                             cTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGAt                                                                                                         >  1:4808612/1‑150 (MQ=255)
                                             cTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGAt                                                                                                         >  2:519183/1‑150 (MQ=255)
                                             cTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGAt                                                                                                         >  2:3691019/1‑150 (MQ=255)
                                             cTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCAGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGAt                                                                                                         <  2:1178021/150‑1 (MQ=255)
                                              tGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATg                                                                                                        >  2:352117/1‑150 (MQ=255)
                                                  ttttGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGc                                                                                                    >  1:1346781/1‑150 (MQ=255)
                                                   tttGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCt                                                                                                   >  2:6356954/1‑150 (MQ=255)
                                                     tGCTGGCATCGGGAACAGGACTGCTTATCTGGCAGGTCTGGAATTTAGTGCGCCTGTCCTGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGc                                                                                                 <  2:2677600/150‑1 (MQ=255)
                                                        tGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGaa                                                                                              <  1:4465499/150‑1 (MQ=255)
                                                        tGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGaa                                                                                              >  1:5203262/1‑150 (MQ=255)
                                                           cATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATa                                                                                           >  2:6063421/1‑150 (MQ=255)
                                                             tCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATaaa                                                                                         >  2:4404503/1‑150 (MQ=255)
                                                             tCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATaaa                                                                                         >  1:2791377/1‑150 (MQ=255)
                                                              cGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATaaaa                                                                                        <  1:5732447/150‑1 (MQ=255)
                                                              cGGTAACAGGACTGCTTATCTGGCATTTCGGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATaaaa                                                                                        <  2:1500325/150‑1 (MQ=255)
                                                               ggTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATaaaaa                                                                                       >  2:4071954/1‑150 (MQ=255)
                                                                gTAACAGGACTGCTTATCTGGCTTTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATaaaaaa                                                                                      <  1:6641924/150‑1 (MQ=255)
                                                                gTAACAGGACTGCTTATCTGGCATTTCTGTAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTCTACGGCTTACACCAGATGCAGCTGCGAAATaaaaaa                                                                                      >  2:2956022/1‑150 (MQ=255)
                                                                gTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATaaaaaa                                                                                      >  2:5597523/1‑150 (MQ=255)
                                                                gTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATaaaaaa                                                                                      <  2:2815289/150‑1 (MQ=255)
                                                                      ggACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAAcgccgc                                                                                <  2:5340441/150‑1 (MQ=255)
                                                                          tGCTTATCTGGCATTTCAGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCTCCGTg                                                                            >  2:6081719/1‑150 (MQ=255)
                                                                             ttATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAAc                                                                         >  1:4063934/1‑150 (MQ=255)
                                                                             ttATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAAc                                                                         >  1:3095743/1‑150 (MQ=255)
                                                                             ttATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAAc                                                                         >  1:5256040/1‑150 (MQ=255)
                                                                                tCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTgg                                                                      <  2:2098645/150‑1 (MQ=255)
                                                                                  tGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACGCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGc                                                                    >  1:4994749/1‑150 (MQ=255)
                                                                                       tttCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCt                                                               <  2:1548822/150‑1 (MQ=255)
                                                                                         tCTGGATTTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATAGGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGa                                                             <  2:845695/150‑1 (MQ=255)
                                                                                           tGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGAtt                                                           >  2:4533347/1‑150 (MQ=255)
                                                                                            ggAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTa                                                          >  2:956549/1‑150 (MQ=255)
                                                                                             gAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTaa                                                         >  2:3259290/1‑150 (MQ=255)
                                                                                              aaTTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTaaa                                                        >  1:5526753/1‑150 (MQ=255)
                                                                                              aaTTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTaaa                                                        >  2:4263588/1‑150 (MQ=255)
                                                                                                    ttGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCttt                                                  >  1:1939502/1‑150 (MQ=255)
                                                                                                     tgggcCTTTCTGGGTGGCTGTGGGTGTATCGCTTTATGACCCCGCCACGGGGGGGTGGTAGCTGGTAACCGCTACTATCCGGCTTACACCTGATGCAGCTGCTAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTc                                                 <  1:4125217/147‑1 (MQ=255)
                                                                                                     tGCGCCTTTCTTGGTGGCTGTGGGTGTATCGCAGTTTGACCCCGCCACGGGGGCGTGGTAGCTGGGAACCGCTACTATAGGGCTTACACCAGATGCAGCTGCTAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTc                                                 <  2:631205/150‑1 (MQ=255)
                                                                                                     tGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTc                                                 <  2:5461536/150‑1 (MQ=255)
                                                                                                     tGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTc                                                 <  2:5145177/150‑1 (MQ=255)
                                                                                                       cgcCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGt                                               >  2:4985066/1‑150 (MQ=255)
                                                                                                           tttctgggtggCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACCCCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAgcg                                           <  2:3074202/144‑1 (MQ=255)
                                                                                                           tttCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAgcg                                           >  2:226086/1‑150 (MQ=255)
                                                                                                           tttCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAgcg                                           >  1:2032584/1‑150 (MQ=255)
                                                                                                             tCATGGTGGCTGTGGGTGGATCGCTGTTTGACCCCGCCACGGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAgcggc                                         <  2:572846/150‑1 (MQ=255)
                                                                                                             tCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAgcggc                                         >  1:3551455/1‑150 (MQ=255)
                                                                                                               ttgggggGTGTGGGTGGATCGCATTTTGCCCCCGCCCCGGGGGGGTGTTTGGTGGCACCCGATACTTTCCGGCTAACACCTTATGCTGCGGATAAAAAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGgcgc                                       <  1:2883655/149‑1 (MQ=255)
                                                                                                               aTGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGgcgc                                       <  2:3994031/150‑1 (MQ=255)
                                                                                                               aTGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGgcgc                                       >  1:3687510/1‑150 (MQ=255)
                                                                                                               aTGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGgcgc                                       >  1:568920/1‑150 (MQ=255)
                                                                                                               aTGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGgcgc                                       >  2:1696541/1‑150 (MQ=255)
                                                                                                                tgggggGTGTGGGTGTATCGCTGTATGACCCGGCCACGGGGGCGTGTCTGGTGGCACCGTATACTTTACGGCTAACACAAGATGCTGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCTGcgcg                                      <  1:3500844/150‑1 (MQ=255)
                                                                                                                tgggggGTGTGGGTGGATCGATTTATGACCCCGCCACGGGGGGGTGGTAGGTGGGAACCGCTACTATAGGGCTTACCCCAGATGCAGCTGATAAAAAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGcgcg                                      <  1:4543836/150‑1 (MQ=255)
                                                                                                                   tggCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAg                                   >  2:5572796/1‑150 (MQ=255)
                                                                                                                     gCTGTGGGTTTATCGAATTATGACCCCGCCACCGGGGCGTTTTAGATGGCAACCGATAATATACGTCTTACACCATATGAAGCTGCTAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTc                                 <  1:965624/150‑1 (MQ=255)
                                                                                                                      cTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCg                                >  2:415673/1‑150 (MQ=255)
                                                                                                                       tgtgGGTGGATCGCTGTATGACCCCGCCACGGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGc                               <  1:157284/150‑1 (MQ=255)
                                                                                                                       tgtgGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGc                               >  1:3962086/1‑150 (MQ=255)
                                                                                                                         tgggtgtATCGATGTATGACCCCGCCACGGGGGGGTGGTAGCTGGGAACCGCTACTATCCGGCTAACACCAGATGAAGCTGCGAAAAAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTg                             <  1:4694037/150‑1 (MQ=255)
                                                                                                                           ggtggATCGCTGTATGACCCCGCCACGGGGGCGTGGTAGCTGGTAACCGATAATATACGGCTTACACCTTATGATGCTGCTAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGcc                           <  1:6356954/150‑1 (MQ=255)
                                                                                                                           ggtggATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGcc                           >  2:6586254/1‑150 (MQ=255)
                                                                                                                                   gCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCgg                   >  2:2479577/1‑150 (MQ=255)
                                                                                                                                   cggttgtGACCCCGCCAGGGGGGGGTGGTAGCTGGTAACCGCTACTATACGGCTAACACCAGATGCAGCTGCGAAATAAAACACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCgg                   <  2:2925438/144‑1 (MQ=255)
                                                                                                                                     aGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCggtg                 >  1:4317882/1‑150 (MQ=255)
                                                                                                                                       tATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCggtggt               <  2:4683862/150‑1 (MQ=255)
                                                                                                                                          taCCACGCCCCGGTGGCGGGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGTAGACGCTGCCCGACGCGGTGGTGCt            >  1:4408984/2‑150 (MQ=255)
                                                                                                                                          taCCACGCCCCGGTGGCGGGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCt            >  1:1071393/2‑150 (MQ=255)
                                                                                                                                          gACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGa                                                             >  1:806784/1‑101 (MQ=255)
                                                                                                                                           aCCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTg           >  2:5916309/1‑150 (MQ=255)
                                                                                                                                             cccGCCCCCGGGGCGTGGTAGCTGGGAACCGCTCCTATACGGCTTACACCAGAGGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAAGCTGATTAAACGCGTTCGGAGCGGCGCGGCGTCGCTGCCCGACGCGGGGGTGCTGAc         >  2:2147053/1‑150 (MQ=255)
                                                                                                                                             cccGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGAc         >  1:179875/1‑150 (MQ=255)
                                                                                                                                                    ccGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGaa  <  1:2106455/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTGCCTCCCGGCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACAGGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGATCGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACACCAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTTCGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAA  >  NC_000913/417939‑418236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: