| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 291 | A→T | 5.5% | intergenic (+36/‑46) | thrL → / → thrA | thr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 291 | 0 | A | T | 5.5% | 1111.3 / 39.1 | 312 | intergenic (+36/‑46) | thrL/thrA | thr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1 |
| Reads supporting (aligned to +/- strand): ref base A (148/145); new base T (11/6); total (159/153) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.49e-01 | |||||||||||
Only 100 of 314 total aligned reads displayed.
ATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCA > NC_000913/142‑439 | ataGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGa > 2:2479478/1‑150 (MQ=255)ataGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGa > 1:3294512/1‑150 (MQ=255) taGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGAc > 1:579041/1‑150 (MQ=255) aGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACa > 1:1097979/1‑150 (MQ=255) cATAGCGCACAGAAATATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACTGTTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTg < 1:3756054/150‑1 (MQ=255) aTAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGc < 2:2873703/150‑1 (MQ=255) cgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGc < 2:5989434/150‑1 (MQ=255) cgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCA‑CTGTCAGGTGCGGGc < 2:587198/150‑1 (MQ=255) gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑t > 1:3496774/1‑150 (MQ=255) acagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑ttttttt > 1:4984953/1‑150 (MQ=255) cataTAAAAATTACAGATTACACAACATCCATGAAACGACTTAGCACCACCATGACCCCCACCACCACCTTTACCACCGGTACGGGTGGGGGCTTACGCTAACAGTAAACACAGAAAAAAGCCCGCAGTTTCTG‑GTGCGGGC‑ttttttct < 1:2959746/147‑3 (MQ=255) aTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCg > 2:1051503/1‑150 (MQ=255) tAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCCCCACCATCCCCATTACCACCGGTAACGGTGCGGGCTGACGCGTAAAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGa < 1:1031582/150‑1 (MQ=255) tAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGa > 2:3629324/1‑150 (MQ=255) tAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGa > 2:334671/1‑150 (MQ=255) aaaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCa > 2:3172149/1‑150 (MQ=255) gagTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCCAAGGTAAc > 2:2006148/1‑150 (MQ=255) cacaACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACTGGAAACACAGAAATTTGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGTCCAATGGTAACGAGGt > 1:3182592/1‑150 (MQ=255) cacaACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGt < 2:3755686/150‑1 (MQ=255) caacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCCCCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTaa < 1:3893695/150‑1 (MQ=255) aacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTaac > 2:1904828/1‑150 (MQ=255) aacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTaac < 1:5952975/150‑1 (MQ=255) aacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTaac < 1:5571987/150‑1 (MQ=255) caTCCATGAATCGCATTAGCACCACCATTACCACCACAATCACCATTACCACAGTTATCGGTGCGGGCTGACGCGTACATTAAAAACAGAAAAAAGCCCGCACCTGACA‑TTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTaacaa < 1:4748322/150‑1 (MQ=255) caTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTaacaa < 1:1426167/150‑1 (MQ=255) aTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTc > 2:497261/1‑126 (MQ=255) cATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCAt > 1:1478216/1‑150 (MQ=255) aTGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATg > 1:268318/1‑150 (MQ=255) aTGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATg > 1:1864407/1‑150 (MQ=255) aaCGCATTAGCACCACCATTACCACCCCCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAg < 1:1935915/150‑1 (MQ=255) aaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAg > 2:1700363/1‑150 (MQ=255) aaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAg > 2:4371129/1‑150 (MQ=255) aCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAgt < 2:6433495/150‑1 (MQ=255) aTTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTg > 2:5578254/1‑150 (MQ=255) aTTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTg > 2:4190690/1‑150 (MQ=255) ttAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGa < 1:5321388/150‑1 (MQ=255) tcgCACCACCAGTCCCACCACCATCACCATTACCACCGGTACCGGTGCGGGCGGCCGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACT‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa < 1:4416654/148‑1 (MQ=255) tAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa < 2:2085677/150‑1 (MQ=255) tAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa < 2:5346533/150‑1 (MQ=255) tAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa < 1:675644/150‑1 (MQ=255) ccaccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcg > 1:183115/1‑150 (MQ=255) caccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTCACAACCATGCGAGTGTTGAAGGTcgg > 2:4676801/1‑150 (MQ=255) caccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcgg < 1:6514977/150‑1 (MQ=255) ttACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTa < 1:3613210/150‑1 (MQ=255) caccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGGTCGGCGGTACATc > 2:4882522/1‑150 (MQ=255) ccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGACGTTCGGCGGTACATCAg > 2:3793177/1‑150 (MQ=255) ccatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTgg < 1:6035251/150‑1 (MQ=255) catcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGc < 2:295046/150‑1 (MQ=255) tcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTT‑TTCGCCCCAAGGTAACGAGGTAACAACCCTGCGGGGGTTGAAGTTCGGCGGTACAACAGTGGCa > 1:3049377/1‑150 (MQ=255) ccatTCCCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACT‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATg < 2:3258965/150‑1 (MQ=255) ccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTACCGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATg > 2:1662194/1‑150 (MQ=255) ataacaacaGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCAGACA‑GTGCGGGC‑TTTTTTTCTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGc > 2:306416/7‑150 (MQ=255) atTACCACAGGTACCGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCCCCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGGACAGCCGTGGCAAATGCa > 1:4529842/1‑150 (MQ=255) atTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCa < 1:549435/150‑1 (MQ=255) ccACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACC‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAc > 1:4309925/1‑150 (MQ=255) ccACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTT‑TTCCACCAACGGTAACGAGGTACCAACCATCCGAGGGTTGAAGTTCGGCGGTACATCATTGGCAATTGCAGaa > 2:3988615/1‑150 (MQ=255) acaGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGt > 1:1242870/1‑150 (MQ=255) gTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCaa < 2:714032/79‑1 (MQ=255) cGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCg > 1:2819565/1‑150 (MQ=255) ggTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCgt < 1:2479478/150‑1 (MQ=255) tGCGGGCTGACGCGGCCAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCgtgt < 2:3413163/150‑1 (MQ=255) ggCGGGCTGTCGCGTACTGGAAACACACAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGTGGTAACAACCATGCGAGTGTTTAAGTTCGGCGGTACATCAGTGGCAAATGCAGATCGTTTTCTGCgtgt < 2:5134568/149‑1 (MQ=255) gggCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGc < 1:5160014/150‑1 (MQ=255) gCTGACGCGTACAGGAAACACAGAATACAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCACAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCg < 2:4283055/150‑1 (MQ=255) cTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGa < 1:952410/150‑1 (MQ=255) aCGCGTACAGGAAACACAGAAAAATGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGTCCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTAGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGatat > 1:1542381/1‑150 (MQ=255) gcgTACAGGAAACACAGAAAAAAGCCCGCCCCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCCAAGGTACCGCGGGAACCACCATGCGAGGGTTGAAGTTCGGCGGGACCACAGGGGCAAATGCCGGCCGGTTTCGGCGTGTTGCCGaacgtc > 1:5256998/1‑145 (MQ=255) gcgTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTc > 2:3105255/1‑150 (MQ=255) gcgTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTc > 2:153821/1‑150 (MQ=255) cgTACAGGAAACACAGAAAAAAGCCCGCACCTTACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAAAAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCTAATTCAGAACGTTTTGTGCGTGTTTGCGATATTCt > 1:2654417/1‑150 (MQ=255) gTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGttt < 1:2198288/128‑1 (MQ=255) gTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGttt > 2:2198839/1‑128 (MQ=255) gTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTg < 2:2516006/150‑1 (MQ=255) gTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTg < 1:1982566/150‑1 (MQ=255) aCAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCACAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGa < 1:6276865/150‑1 (MQ=255) cAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGaa < 2:1591453/150‑1 (MQ=255) aGGAAACACAGAAAAATGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGTAGTTCGGCGGTACAGCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGaaa > 2:6203349/1‑150 (MQ=255) acacAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAAt > 2:530480/1‑150 (MQ=255) gaaaaaaaaaGCCCGCA‑CTGTCAGGTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGaa < 1:3157145/138‑1 (MQ=38) acaGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGc > 2:55395/1‑150 (MQ=255) acaGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGc < 1:2063963/150‑1 (MQ=255) acaGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGc > 2:6556197/1‑150 (MQ=255) aaaaaaaaaGCCCGCA‑CTGTCAGGTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTg > 2:3565358/4‑138 (MQ=38) aGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCca < 2:1242870/150‑1 (MQ=255) aGAAAAAAGCCCGAACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCca < 1:495498/150‑1 (MQ=255) gAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcag < 1:6663898/150‑1 (MQ=255) aaaaaaGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcagg > 2:1730430/1‑150 (MQ=255) aaaaaaGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcagg < 1:4190690/150‑1 (MQ=255) aaaaGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggca < 2:54061/150‑1 (MQ=255) aaaGCCCGCA‑CTGTCAGGTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggcag > 2:6040029/1‑150 (MQ=255) aaGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggcagg < 2:1480886/150‑1 (MQ=255) aaGCCCGCA‑CTGTCAGGTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggcagg > 2:3302720/1‑150 (MQ=255) aGCCCGCA‑CTGTCAGGTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCTGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggg > 2:86178/1‑150 (MQ=255) gCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcagggg > 2:2385618/1‑150 (MQ=255) ccGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggca > 2:1865063/1‑150 (MQ=255) ccGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTAAATCAGTGGCAAATGCAGAACGTTTTATGCGTGTTTCCGATATTCTGGAAAGCAATGCCAggcaggggca > 1:5102858/1‑150 (MQ=255) cGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTAGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggcag > 1:882914/1‑150 (MQ=255) cACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGt < 1:3090262/150‑1 (MQ=255) aCCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTg < 2:2931537/150‑1 (MQ=255) ccTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTgg < 2:268318/150‑1 (MQ=255) | ATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑TTTTTTT‑TTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCA > NC_000913/142‑439 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |