Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,792,241 A→T 7.6% intergenic (‑221/+26) ydiV ← / ← nlpC anti‑FlhD4C2 factor, inactive EAL family phosphodiesterase/putative C40 clan peptidase lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,792,2410AT 7.6% 907.2 / 46.1 274intergenic (‑221/+26)ydiV/nlpCanti‑FlhD4C2 factor, inactive EAL family phosphodiesterase/putative C40 clan peptidase lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (132/121);  new base T (10/11);  total (142/132)
Fisher's exact test for biased strand distribution p-value = 8.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

CAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGC  >  NC_000913/1792094‑1792390
                                                                                                                                                   |                                                                                                                                                     
   aaGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTatcatc                                                                                                                                                  >  1:2997563/1‑150 (MQ=255)
   aaGGAGTAACTTTCATTTGTTCCATGTTAACCACTGTATCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATATACGCAAATTTAGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTatcatc                                                                                                                                                  >  2:5300507/1‑150 (MQ=255)
     ggAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGc                                                                                                                                                <  2:3160059/150‑1 (MQ=255)
       aGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCg                                                                                                                                              >  2:1823757/1‑150 (MQ=255)
        gTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGt                                                                                                                                             <  2:1635915/150‑1 (MQ=255)
        gTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGATACATCGCCGt                                                                                                                                             <  2:4510958/150‑1 (MQ=255)
        gTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGATACATCGCCGt                                                                                                                                             <  2:4617340/150‑1 (MQ=255)
        gTAACTTTCATTTATTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGATACATCGCCGt                                                                                                                                             <  2:2412944/150‑1 (MQ=255)
         tAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTc                                                                                                                                            >  1:4347658/1‑150 (MQ=255)
           aCTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCa                                                                                                                                          >  2:1060050/1‑150 (MQ=255)
            cTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAAGAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGATACATCGCCGTCCAt                                                                                                                                         <  1:3624480/150‑1 (MQ=255)
             tttCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATAATAAACGAATAATCCATTTACGGAATTTTTGTATGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCAtt                                                                                                                                        >  1:5974575/1‑150 (MQ=255)
              ttCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTccctgt                                                                                                                                       >  2:5710883/1‑146 (MQ=255)
              ttCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCAttt                                                                                                                                       >  2:91615/1‑150 (MQ=255)
              ttCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCAttt                                                                                                                                       <  2:4829384/150‑1 (MQ=255)
              ttCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCAttt                                                                                                                                       >  2:5710818/1‑150 (MQ=255)
                cATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGATACATCGCCGTCCAtttac                                                                                                                                     <  2:1592957/150‑3 (MQ=255)
                 aTTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCAGTTTTc                                                                                                                                    >  2:5824137/1‑150 (MQ=255)
                  tttGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCg                                                                                                                                   >  1:4498001/1‑150 (MQ=255)
                        ccATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATAc                                                                                                                             <  2:4130980/150‑1 (MQ=255)
                         cATTTTAACCACTTTTTCAGGGGTCATTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTTTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACt                                                                                                                            <  1:4249368/150‑1 (MQ=255)
                         cATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCAGTTTTCGACATACt                                                                                                                            >  2:3598070/1‑150 (MQ=255)
                          aTGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTa                                                                                                                           >  1:2493977/1‑150 (MQ=255)
                          aTGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTa                                                                                                                           >  1:5901678/1‑150 (MQ=255)
                            gTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTATGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATAATCGCCGTCCATTTTTCGACATACTAGa                                                                                                                         >  1:3881728/1‑150 (MQ=255)
                                 ccACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGt                                                                                                                    >  1:3997660/1‑150 (MQ=255)
                                 ccACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGt                                                                                                                    >  2:6110817/1‑150 (MQ=255)
                                  cACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAATTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATAATAGATTAGtc                                                                                                                   >  1:5283955/1‑150 (MQ=255)
                                     tttttCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCg                                                                                                                >  1:1633369/1‑150 (MQ=255)
                                     tttttCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCg                                                                                                                >  1:6138213/1‑150 (MQ=255)
                                          cAGGGGTTCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGc                                                                                                           <  2:4592169/150‑1 (MQ=255)
                                          cAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGc                                                                                                           <  2:6243543/150‑1 (MQ=255)
                                           cggggTCCTTTTTTTAGATTATCCTGATTATAAACGACTAAGCCATTTCCGGAATTTTTGTCTGCAAAATACTACTGTATTCCGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGcc                                                                                                          <  1:5680653/149‑1 (MQ=255)
                                           aGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGcc                                                                                                          >  2:5123394/1‑150 (MQ=255)
                                             gggTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAg                                                                                                        >  2:3953875/1‑150 (MQ=255)
                                                 ccTTTCTTTAGATCATCCGGATAATAAACGAACAACCCATTTACGGAATTTTTGCCCGCAAAATACTACGGTATCCCGGAGTAAATGGACGGCGATGTATCATCGCCGTCCAATTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAAt                                                                                                    <  1:2909155/150‑1 (MQ=255)
                                                  cTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAAtt                                                                                                   >  2:1799413/1‑150 (MQ=255)
                                                   tttttttAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAAttt                                                                                                  >  2:1974788/1‑150 (MQ=255)
                                                   tttttttAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAAttt                                                                                                  >  1:1516180/1‑150 (MQ=255)
                                                   tttttttAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAAttt                                                                                                  >  2:1015894/1‑150 (MQ=255)
                                                     tttttAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAAttttt                                                                                                <  1:11627/150‑1 (MQ=255)
                                                          aGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGcc                                                                                           >  2:3484707/1‑150 (MQ=255)
                                                            aTTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCaa                                                                                         >  1:2034868/1‑150 (MQ=255)
                                                            aTTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGATACATCGCCGt                                                                                                                                             <  2:3791185/98‑1 (MQ=255)
                                                               aTCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCTATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAg                                                                                      >  1:428818/1‑150 (MQ=255)
                                                                  cTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACa                                                                                   >  1:2617739/1‑150 (MQ=255)
                                                                  cTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACa                                                                                   >  1:6326193/1‑150 (MQ=255)
                                                                         taAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGATTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCaa                                                                            <  1:5818150/150‑1 (MQ=255)
                                                                         taAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCaa                                                                            >  1:3209487/1‑150 (MQ=255)
                                                                           aaCGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGt                                                                          >  2:4928765/1‑150 (MQ=255)
                                                                            aCGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATATACATTATCAAGTg                                                                         >  1:5529963/1‑150 (MQ=255)
                                                                             cGAATAATCCATTTACGGAAATTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGa                                                                        <  1:2743747/150‑1 (MQ=255)
                                                                               aataatCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTATGCCAATAGACATTATCAAGTGAgg                                                                      >  1:654208/1‑150 (MQ=255)
                                                                                  aatCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAAc                                                                   <  2:3752766/150‑1 (MQ=255)
                                                                                   atCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACg                                                                  <  2:4466920/150‑1 (MQ=255)
                                                                                       aTTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTATGCCAATAGACATTATCAAGTGAGGAACGCATc                                                              >  1:838525/1‑150 (MQ=255)
                                                                                        tttACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCa                                                             <  2:5035542/150‑1 (MQ=255)
                                                                                            cGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTcc                                                         >  1:4877720/1‑150 (MQ=255)
                                                                                             ggAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTccc                                                        >  2:6678259/1‑150 (MQ=255)
                                                                                              gAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTAGTCTCGCCTGCCAGAAATTTTTATGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCt                                                       >  1:4622686/1‑150 (MQ=255)
                                                                                              gAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATAATAGATTCGTCACGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCt                                                       >  1:4697489/1‑150 (MQ=255)
                                                                                                  ttttGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCt                                                   <  1:6678089/150‑1 (MQ=255)
                                                                                                    ttGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTgg                                                 <  1:2477402/150‑1 (MQ=255)
                                                                                                    ttGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCCTCCATTTTTAGACAAACTAGATTCGTCTCGCCAGCCAGAAAATTTTATGCCAATAGACATTATCAAGTGAGGAACGCAACACTCCCGTGCTgg                                                 >  1:2805043/1‑150 (MQ=255)
                                                                                                         tGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTagag                                            <  1:1823757/150‑1 (MQ=255)
                                                                                                         tGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTagag                                            >  2:5779088/1‑150 (MQ=255)
                                                                                                         tGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTagag                                            <  1:981491/150‑1 (MQ=255)
                                                                                                         tGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACGAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTagag                                            <  2:5974575/150‑1 (MQ=255)
                                                                                                         cgCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTagag                                            <  1:516382/149‑1 (MQ=255)
                                                                                                            aaaaTACTACTGTATTCAGGAGTAAATGGACGGCGATGTTTCATCGCCGTCCATTTTTCGACATAATAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAATTGAGGAACGCATCACACCCATGCTGGTTGAGGCg                                         >  1:2269148/1‑150 (MQ=255)
                                                                                                            aaaaTACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCg                                         >  2:4260924/1‑150 (MQ=255)
                                                                                                            aaaaTACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAAATTTTCTGCCAATAGTCATTATCAAGTGAGGAACGCATCACTCCCGTGCTGGTCGAGGCg                                         >  1:873172/1‑150 (MQ=255)
                                                                                                             aaaTACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGt                                        <  1:4792883/150‑1 (MQ=255)
                                                                                                                     ctGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAAt                                >  2:6460554/1‑150 (MQ=255)
                                                                                                                     ctGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAAt                                <  2:4630618/150‑1 (MQ=255)
                                                                                                                     ctGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAAt                                >  2:6317788/1‑150 (MQ=255)
                                                                                                                      tGTATTCAGGAGTAAATGGAAGGCGATGTATAATAGCCGTCCATTTTTCGACATACTAGATTAGTCTCGCCAGCCAGAAATTTTTATTCCAATAGACATTATAAAGTGTGGAACGCATCACTCCCGTGCGGGTAGAGGCGTGGATAAAtt                               >  1:3909316/1‑150 (MQ=255)
                                                                                                                       gTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATATATTg                              <  2:2830471/150‑1 (MQ=255)
                                                                                                                       gTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTg                              <  2:3212897/150‑1 (MQ=255)
                                                                                                                         aTTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgt                            <  1:1464064/150‑1 (MQ=255)
                                                                                                                         aTTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgt                            <  1:3764802/150‑1 (MQ=255)
                                                                                                                             aGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgttgtt                        <  1:1974788/150‑1 (MQ=255)
                                                                                                                             aGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgttgtt                        <  2:250393/150‑1 (MQ=255)
                                                                                                                             aGGAGTAAATGGACGGCGATGTATAAACGCCGTCCAATTTTCGACATAATAGATTAGTATCGCCAGCCAGAAAATTTTCTGCCAATATACAATATAAAGTGAGGAACGCAACACACCCATGCTGGTAGAGGCGTGGGTAAATTTtttgtt                        >  1:4159631/1‑150 (MQ=255)
                                                                                                                              ggAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGCCATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGttgttg                       <  2:4672382/150‑1 (MQ=255)
                                                                                                                              ggAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGttgttg                       <  2:1347218/150‑1 (MQ=255)
                                                                                                                              ggAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGttgttg                       <  1:6408492/150‑1 (MQ=255)
                                                                                                                              ggAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGttgttg                       <  2:1516180/150‑1 (MQ=255)
                                                                                                                              ggAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGttgttg                       <  2:1875250/150‑1 (MQ=255)
                                                                                                                               gAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTgg                      >  2:298589/1‑150 (MQ=255)
                                                                                                                                aGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGt                     <  2:1719239/150‑1 (MQ=255)
                                                                                                                                 gTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTa                    <  2:818910/150‑1 (MQ=255)
                                                                                                                                  tAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGaa                                                                    <  1:1248767/101‑1 (MQ=255)
                                                                                                                                   aaaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATc                  <  2:4185481/150‑1 (MQ=255)
                                                                                                                                    aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCg                 >  2:3400402/1‑150 (MQ=255)
                                                                                                                                     aTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGt                <  1:1015642/150‑1 (MQ=255)
                                                                                                                                       ggACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTaa              >  2:400163/1‑150 (MQ=255)
                                                                                                                                         aCGGCGATGATACATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAAt            >  2:2583263/1‑150 (MQ=255)
                                                                                                                                         aCGGCGATGATACATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAAt            >  2:4397236/1‑150 (MQ=255)
                                                                                                                                           ggCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATAc          <  2:5357258/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGC  >  NC_000913/1792094‑1792390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: