Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,074,691 C→G 5.1% intergenic (+33/‑88) flu → / → yeeR CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter/CP4‑44 prophage; putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,074,6910CG 5.1% 1144.4 / 36.8 332intergenic (+33/‑88)flu/yeeRCP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter/CP4‑44 prophage; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base C (150/159);  new base G (5/12);  total (157/175)
Fisher's exact test for biased strand distribution p-value = 1.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 361 total aligned reads displayed.

GAAGCCCGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCATTCTTCGCCTTTT  >  NC_000913/2074542‑2074840
                                                                                                                                                     |                                                                                                                                                      
gAAGCCCGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGtcatc                                                                                                                                                        <  2:2101449/150‑1 (MQ=255)
gAAGCCCGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGtcatc                                                                                                                                                        <  2:715265/150‑1 (MQ=255)
     ccGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGa                                                                                                                                                    >  2:816649/1‑149 (MQ=255)
      cGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATGATGAcc                                                                                                                                                  <  2:2967193/150‑1 (MQ=255)
       gtgtCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATGATGACCg                                                                                                                                                 <  1:2786428/150‑1 (MQ=255)
         gtCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGATGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCggg                                                                                                                                               <  1:1039946/150‑1 (MQ=255)
         gtCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATGATGACCggg                                                                                                                                               <  1:3887441/150‑1 (MQ=255)
         gtCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCggg                                                                                                                                               <  1:3669903/150‑1 (MQ=255)
          tCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGtcatca                                                                                                                                                       <  1:3253287/141‑1 (MQ=255)
          tCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATGATGACCGGGa                                                                                                                                              <  2:2430337/150‑1 (MQ=255)
          tCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGa                                                                                                                                              <  2:918584/150‑1 (MQ=255)
           ccGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGAc                                                                                                                                             <  2:1039844/150‑1 (MQ=255)
             gggAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGAccc                                                                                                                                           >  2:3168719/1‑150 (MQ=255)
              ggAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTCACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCAGGTCATCATGACCGGGACCCg                                                                                                                                          >  2:2726489/1‑150 (MQ=255)
                aaaaTATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGa                                                                                                                                        <  2:6629013/150‑1 (MQ=255)
                     atCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGTTATAACGGTCAGGCCACACTGAATTTGACCTTATGAAAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGgc                                                                                                                                   <  2:1316867/150‑1 (MQ=255)
                     atCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGgc                                                                                                                                   <  2:3807224/150‑1 (MQ=255)
                      tCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAAAGGTCAGGCCACACTGAATGTGACCTTCTGACAGAAACAACGCCTCTCTGTGGTCCCCGTCCTCAAGAACCGGACCCCGACCcgcg                                                                                                                                  >  1:2564556/1‑150 (MQ=255)
                        aCCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGGGGTCCCGGTCATGATGACCGGGAccacaga                                                                                                                                       <  2:4046693/143‑6 (MQ=255)
                          ccTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAAc                                                                                                                              >  1:139285/1‑150 (MQ=255)
                          ccTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAAc                                                                                                                              >  2:6141190/1‑150 (MQ=255)
                           cTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACg                                                                                                                             >  1:5610922/1‑150 (MQ=255)
                            tGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCCACgg                                                                                                                            >  1:1815029/1‑150 (MQ=255)
                            tGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACgg                                                                                                                            <  2:4299155/150‑1 (MQ=255)
                             gggCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGa                                                                                                                           >  1:6604824/1‑150 (MQ=255)
                             gggCGTTCAGGCCGGTTAGGCCCACCGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGACCCATCGCCTCTCTGTGGGCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGa                                                                                                                           <  2:4795996/150‑1 (MQ=255)
                              ggCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGAt                                                                                                                          >  1:2393378/1‑150 (MQ=255)
                                cGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCt                                                                                                                        >  2:5204732/1‑150 (MQ=255)
                                 gTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCCTGACCGGGACCCGGACCGGCGCAACGGATCtt                                                                                                                       >  2:1797753/1‑150 (MQ=255)
                                 gTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCtt                                                                                                                       >  2:377116/1‑150 (MQ=255)
                                  ttCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTc                                                                                                                      >  2:4327328/1‑150 (MQ=255)
                                        ccGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGACTGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGCCCCGGACCGGCGCACCGGCTCTTCAACGcc                                                                                                                >  1:542185/1‑150 (MQ=255)
                                        ccGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGCCCCGGACCGGCGCAACGGATCTTCAACGcc                                                                                                                >  2:1993769/1‑150 (MQ=255)
                                          ggTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAATGGTATAAAGGTCAGTCCACACTGAATGTGACCTTCTGACAGAACAATCGCATCTCTGTGGTCACGGTAATCATGACCGGGACCCGGACCGGCGCAACGGATATTCaaagacaa                                                                                                              >  1:1169544/1‑144 (MQ=255)
                                              aTGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTc                                                                                                          <  2:6399519/150‑1 (MQ=255)
                                              aTGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTc                                                                                                          >  1:341692/1‑150 (MQ=255)
                                                 cccACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCt                                                                                                       <  2:3452043/150‑1 (MQ=255)
                                                  ccACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTg                                                                                                      >  1:5121743/1‑150 (MQ=255)
                                                  ccACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTg                                                                                                      >  1:4427140/1‑150 (MQ=255)
                                                   cacaGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATTATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTgg                                                                                                     >  1:6078507/1‑150 (MQ=255)
                                                         gTCCGCGGCAGCTGGGCTGATGGGTATACGGGTCTGGCCACACTGATTGTGACCTTCTGACACAACCAGCGCCCCTCTGTGGTCCCGGTCATCTTGCCCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTaa                                                                                               <  1:418475/150‑1 (MQ=255)
                                                             gCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAAt                                                                                           >  2:4860772/1‑150 (MQ=255)
                                                               ggCAGCCGCGCTGATGGGTATAACGGCCTGGCCACCCTTAATGTGACCTTCTGACAGAACAATCGCCTCTCTGTGGTCCCGGTAATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATaa                                                                                         <  1:2246493/150‑1 (MQ=255)
                                                               ggCAGCAGCGCTGAAGGTTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATaa                                                                                         <  2:120172/150‑1 (MQ=255)
                                                                 cagcagCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAAAAACa                                                                                       >  2:3680440/1‑150 (MQ=255)
                                                                  agcagcGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACAt                                                                                      <  2:3582055/150‑1 (MQ=255)
                                                                      gcgcTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCCCATTCGCTGGCATTAACAATAACATGata                                                                                  >  1:1526935/1‑150 (MQ=255)
                                                                      ccgcTTAAGGTTAAAACGGACAGCCCAAACTCAATGTCACATACTCACACAACCATCCCCACTCTGTGGTCCCGGAAAACATCACCGGCACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGata                                                                                  <  1:6363358/149‑1 (MQ=255)
                                                                        gcTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGAGAtt                                                                                >  2:6039510/1‑150 (MQ=255)
                                                                         cTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACAAGATATtc                                                                               >  2:5114410/1‑150 (MQ=255)
                                                                          tGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATtca                                                                              >  1:5216280/1‑150 (MQ=255)
                                                                             aGGTTATAACGGCCAGGCCACACTTAATGTGACCTTCTGACATAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACGGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATtcatca                                                                           <  1:3308597/150‑1 (MQ=255)
                                                                              gggTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCAc                                                                          >  1:474405/1‑150 (MQ=255)
                                                                               ggTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGGCCCGGTCATCTTGACGGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACg                                                                         <  1:5745550/150‑1 (MQ=255)
                                                                                     aCGGTCAGGCCACACTGTTTGTGACCTTCTGACAGAACCTTCGCCTCTCTGTGGTCCCGGTCATCATGTCCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGa                                                                   <  1:6571818/150‑1 (MQ=255)
                                                                                     aCGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGa                                                                   <  2:5782705/150‑1 (MQ=255)
                                                                                     aCGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGa                                                                   <  2:2209938/150‑1 (MQ=255)
                                                                                       ggTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACt                                                                 <  2:5377124/150‑1 (MQ=255)
                                                                                       ggTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACt                                                                 >  1:3576182/1‑150 (MQ=255)
                                                                                                acacTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAg                                                        >  2:5026885/1‑150 (MQ=255)
                                                                                                acacTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAAAAACAAGATATTCATCACGGAGTGACTATGTTACAg                                                        >  1:299338/1‑150 (MQ=255)
                                                                                                acacTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCCGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAg                                                        >  2:2451321/1‑150 (MQ=255)
                                                                                                    tGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAg                                                    >  2:4108008/1‑150 (MQ=255)
                                                                                                        tgtgACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATTACCGGGACCCGGACCGGCGCAACGGATCTTCAAAGCCACAATCGCTGGCATTAACAATAACATGATATTCATAAAGGAGTGACTATGTTACAGATATTCgg                                                >  1:4648867/1‑150 (MQ=255)
                                                                                                        tgtgACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCgg                                                >  2:5317387/1‑150 (MQ=255)
                                                                                                             ccTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgcgcgc                                           <  2:4427140/150‑1 (MQ=255)
                                                                                                             ccTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgcgcgc                                           <  1:925093/150‑1 (MQ=255)
                                                                                                              cTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCt                                          <  1:1563052/150‑1 (MQ=255)
                                                                                                                 cTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGAt                                       <  2:5410925/150‑1 (MQ=255)
                                                                                                                  tGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATc                                      <  2:3922962/150‑1 (MQ=255)
                                                                                                                  tGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATc                                      <  2:194357/150‑1 (MQ=255)
                                                                                                                  tGACAGAACCATCGCCTCTCTGTGGTCCCCGTCATCATGACCGGGACCCCGACCGGCGCAACGGATCTTCAAAGCCACATTCGCTGGCATTAACAATAAAATGATATTAATCAAGGAGTGACTATGTTAAATATATTCGGCGCGCGGATc                                      >  1:1703338/1‑150 (MQ=255)
                                                                                                                     cAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCctg                                   <  2:5729702/150‑1 (MQ=255)
                                                                                                                      aGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCctgc                                  <  2:470085/150‑1 (MQ=255)
                                                                                                                       gacccATCGCCTCTCTGGGGCCCCGGTCATCAGGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCctgct                                 <  1:721118/147‑1 (MQ=255)
                                                                                                                       gAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCctgct                                 <  1:2290249/150‑1 (MQ=255)
                                                                                                                       gAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCctgct                                 <  2:5846531/150‑1 (MQ=255)
                                                                                                                          ccATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGAt                              <  1:4777035/150‑1 (MQ=255)
                                                                                                                           cATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATTATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATc                             <  1:4327328/150‑1 (MQ=255)
                                                                                                                           cATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATc                             >  2:2291701/1‑150 (MQ=255)
                                                                                                                           cATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATc                             <  1:4937514/150‑1 (MQ=255)
                                                                                                                              cGCCTCTCTGTGGTCCCGGTCATCATGCCCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCa                         <  1:2334704/150‑1 (MQ=255)
                                                                                                                              cGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCa                         <  1:3785339/150‑1 (MQ=255)
                                                                                                                               gCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAg                        <  1:6144446/150‑1 (MQ=255)
                                                                                                                                 ctctctGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGa                      <  1:2130750/150‑1 (MQ=255)
                                                                                                                                      tgtgGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGc                 <  2:1680261/150‑1 (MQ=255)
                                                                                                                                      tgtgGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCGATCAGCAGGAtcagc                 <  2:6604824/150‑5 (MQ=255)
                                                                                                                                            cccGGTCATGATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCAttct           >  2:1522598/1‑150 (MQ=255)
                                                                                                                                            cccGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCAttct           >  2:1845123/1‑150 (MQ=255)
                                                                                                                                             ccGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCAttctt          >  2:5532548/1‑150 (MQ=255)
                                                                                                                                             ccGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCAttctt          >  1:3078200/1‑150 (MQ=255)
                                                                                                                                             ccGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATACATCACGGAGAGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATATGCCAttctt          >  2:2681111/1‑150 (MQ=255)
                                                                                                                                             ccGGTCATCATGACCGGGACCCGGACAGGCGCAACTGATCTTCAACGACACAATCGATGGCATTAACAATAAAATGAAAATCATCACGGAGTGACTATGTTAAAGATAGTAGGAGAGCTGATCCTGATGATCGAAGTATTTGACAatctt          >  1:962880/1‑150 (MQ=255)
                                                                                                                                               ggTCATGATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCATTCTTCg        >  2:5988788/1‑150 (MQ=255)
                                                                                                                                               ggTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGTGCGCTGATCCTGCTGATCGCAGGATTTGCCATTCTTCg        <  2:5927383/150‑1 (MQ=255)
                                                                                                                                                gTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCATTCTTCGc       <  1:4909413/150‑1 (MQ=255)
                                                                                                                                                   atcatGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAAAAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCATTCTTCGCCtt    >  2:1594329/1‑150 (MQ=255)
                                                                                                                                                   aacatGACCGGGACCCGGACCGGCGCAACGGAACTTCAACGCCACATTCGCTGGCATAAACAAAAACAAGATAATCATCACGGAGTGACTATGGTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCACGATTTGCCATTCTTCGCCtt    >  2:3123287/3‑150 (MQ=255)
                                                                                                                                                    tcatGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCATTCTTCGCCttt   >  2:6209154/1‑150 (MQ=255)
                                                                                                                                                     catGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCATTCTTCGCCtttt  <  1:189396/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GAAGCCCGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCATTCTTCGCCTTTT  >  NC_000913/2074542‑2074840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: