Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,245 G→T 13.2% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2450GT13.2% 802.3 / 22.2 329intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (129/138);  new base T (31/26);  total (160/169)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 336 total aligned reads displayed.

TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  NC_000913/2177096‑2177394
                                                                                                                                                      |                                                                                                                                                       
tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATg                                                                                                                                                         <  1:3902989/150‑1 (MQ=255)
    tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAA‑AAAAGaaaa                                                                                                                                                     >  2:3009963/1‑149 (MQ=255)
    tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATA‑TAATTAAat                                                                                                                                                     >  1:6283328/1‑150 (MQ=255)
     accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAatt                                                                                                                                                    >  1:1489963/1‑150 (MQ=255)
     accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATA‑TAATGaaaat                                                                                                                                                    >  1:6545971/1‑148 (MQ=255)
      ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAatta                                                                                                                                                   >  1:2268097/1‑150 (MQ=255)
        accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAattatt                                                                                                                                                 >  2:1832867/1‑150 (MQ=255)
           acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGt                                                                                                                                              <  1:4882231/150‑1 (MQ=255)
             ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGttt                                                                                                                                            <  2:1155081/150‑1 (MQ=255)
             ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGttt                                                                                                                                            <  2:5163765/150‑1 (MQ=255)
               gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATA‑TAATTAAAtttttttttttt                                                                                                                                          >  2:5640349/1‑150 (MQ=255)
               gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAATA‑TAAAGAAATTATtttttttt                                                                                                                                          >  2:2312458/1‑150 (MQ=255)
                cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTa                                                                                                                                         <  2:3033645/150‑1 (MQ=255)
                 aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTaa                                                                                                                                        <  1:2967949/150‑1 (MQ=255)
                 aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATA‑TAATGAAAATATTTTTTTTTaa                                                                                                                                        >  1:3236395/1‑150 (MQ=255)
                     tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATA‑TAATTAAATTATTTTTTTTTAAatat                                                                                                                                    >  1:184230/1‑150 (MQ=255)
                      ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATc                                                                                                                                   <  1:1641489/150‑1 (MQ=255)
                      ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAA‑TAATGAAATAATTCGTTTTTAAATATc                                                                                                                                   >  1:5965995/1‑150 (MQ=255)
                      ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAA‑TAAATAAAATATTTTTTTTTAAataac                                                                                                                                   >  1:873895/1‑148 (MQ=255)
                      ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATA‑TAATTAAATTATTAGTTTTTAAatata                                                                                                                                   >  1:6475344/1‑149 (MQ=255)
                        cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCga                                                                                                                                 <  2:6683046/150‑1 (MQ=255)
                         gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAAATAAAATATTTTTTTTTAAATATagag                                                                                                                                >  2:2937663/1‑150 (MQ=255)
                          agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCgaga                                                                                                                               <  1:6412127/150‑1 (MQ=255)
                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAA‑TAATGAAATTATTTTTTTTTAAATATCGAGATAAAGAt                                                                                                                        >  2:2547095/1‑150 (MQ=255)
                                    tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAattatt                                                                                                                                                 >  2:4726140/1‑122 (MQ=255)
                                    tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAattatt                                                                                                                                                 <  1:4726140/122‑1 (MQ=255)
                                        gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaa                                                                                                                 <  2:1967250/150‑1 (MQ=255)
                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                                <  1:2476446/150‑1 (MQ=255)
                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                                <  2:6285559/150‑1 (MQ=255)
                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                                <  1:5898178/150‑1 (MQ=255)
                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                                <  1:5796662/150‑1 (MQ=255)
                                          aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                                               <  2:6547526/150‑1 (MQ=255)
                                          aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAA‑TAATGAAATTATTTTTTTTTAAATATAGCGATAACGATCACaaaaaa                                                                                                               >  2:1374739/1‑149 (MQ=255)
                                            gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                             <  2:763596/150‑1 (MQ=255)
                                            gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAAATATTTGGTTTAAAATATCGAGATAACGATAACAAAAAAGa                                                                                                             >  2:6392405/1‑150 (MQ=255)
                                            gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTACAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                             <  2:794206/150‑1 (MQ=255)
                                                       aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATA‑TAATGAAATTATTTTTTTTTAAATATAGAGATAAAGATCACAAAAACGCCAATATGacaa                                                                                                  >  2:4171768/1‑150 (MQ=255)
                                                             gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTc                                                                                            <  2:3177725/150‑1 (MQ=255)
                                                              ccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAA‑AAATTAAAATATTTTTTTTTAAATATATAGATAAAGATAAAAAAAAAAACAAAAAGAAAaatattag                                                                                           >  1:2872145/1‑148 (MQ=255)
                                                                  gtgtggCGTTGTTCACCATGTGCTTTTTCGATACCACTTAATTTTTCTTATCCTGTGTTTTGTTTTCGATTTAAAAATA‑TAATCAAATTTTTTTTTTTTAAATATCGAGATAACTATCAAAAAAACGACAATATGAAAATTATTCgagga                                                                                       <  1:3458667/145‑1 (MQ=255)
                                                                      tgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAA‑AAATTAAAATATTTTTTTTTTAATAAATAGATAAAGAAAAAAAAAAAAACAAAAATAAAAATATTATAGGAGttg                                                                                   >  1:12713/1‑148 (MQ=255)
                                                                        cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                                 >  2:5544127/1‑150 (MQ=255)
                                                                           tgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑Gc                                                                             <  2:4098901/150‑1 (MQ=255)
                                                                               cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATA‑AAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAAAGAAAATATTTAAAATATTAGAGGAGTGAAAG‑GC‑aaaa                                                                        >  2:2740702/1‑150 (MQ=255)
                                                                                  cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATA‑TAATGAAATTATTTGTTTTTAAATATAGAGATAAAGATAACAAAAAAGAAAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑aaaaaaa                                                                     >  2:5811431/1‑150 (MQ=255)
                                                                                  cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATA‑TAAATTAAATATTTGTTTTTTTATATAGAGATAACGAAAACAAAAAACACAAAATTTAAAATAATAGAGGAGTGAAAG‑GC‑ccaaaaa                                                                     >  1:409406/1‑150 (MQ=255)
                                                                                   aTCTGCTTTGTCGGTACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGTATTAAAAAAA‑TAATTAAAATATTTTTTTTTAAATAAAGAGATAACGAAAACAAAAAACACAAAAATAAAAATATTAGAGGAGTGTAAGTGC‑aaaaaaa                                                                     >  1:1098597/1‑150 (MQ=255)
                                                                                   aTCTGCTTTGTCGATACCACGTACATTTTCATCTCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAAc                                                                    <  2:1542718/150‑1 (MQ=255)
                                                                                      tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATA‑TAATGAAAATATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAAAGGc                                                                 >  2:5318248/1‑150 (MQ=255)
                                                                                             tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGa                                                          >  2:3881719/1‑150 (MQ=255)
                                                                                             tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATA‑TAATGAAATTATTTGTTTTTAAATATGGAGATAACGATCACAAAAACGACAATATGTAAATTATTCGAGGAGTGTAAG‑GC‑AAAAAAAAGGCCTCCCGa                                                          >  2:3224133/1‑150 (MQ=255)
                                                                                              ctacaCCACGTACTTTTTCATATCCTTTCTTTTTTTTTCTATTAAAAAATA‑TAATTAAATTATTTGTTTTTAAATATCGATATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGAt                                                         <  1:212016/146‑1 (MQ=255)
                                                                                               gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAAAGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAAAGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAAAGGCCTCCCGATa                                                        >  2:4023305/1‑150 (MQ=255)
                                                                                                  aCCACTTACATTTTCATATCCTGTCGTTTGTTTTCGATTACAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAggg                                                     <  2:1319455/150‑1 (MQ=255)
                                                                                                         aaaTTTTCATATCCTGTCTTTTTTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGt                                              <  1:5053216/148‑1 (MQ=255)
                                                                                                         aCATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCTTCCCTATCGGGAGGCCGt                                              <  2:6093648/150‑1 (MQ=255)
                                                                                                         aCATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAAAATGTAAATTATTGGTGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGt                                              >  2:4785888/1‑150 (MQ=255)
                                                                                                         aCATTTACATATCCTGTGGTTTTTTTTC‑TTTTCAAAAAATTAATCAAATTATTTTTTTTTAATTATCTTTATAACTATCAAAAAAACGAAAATATGAAAATTATTCGAGGTGTTAAAG‑GC‑AAAAAAACGGCTTCCCTATCGGGAGGCCGt                                              <  1:2746963/150‑1 (MQ=255)
                                                                                                          cATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                             <  2:5454445/150‑1 (MQ=255)
                                                                                                          cATTTTCATATCCGGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                             <  2:4373945/150‑1 (MQ=255)
                                                                                                          cATTTACCTATCCTGGGGTTTGTTTTCGTTTTCAACTTA‑TACTGAATTTATTTGTTTTTAAATATCGCGATAAGGATCACAAAACCGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                             <  1:5617448/150‑1 (MQ=255)
                                                                                                          aaTTTTAATATCCTGTCGTTTGTTTTCTATTTAAAAATA‑TAATTAAATTTTTTTTTTTTAAATATCGTGATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                             <  1:3131376/149‑1 (MQ=255)
                                                                                                           aTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTTTTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GCAAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                             <  2:3483451/150‑1 (MQ=255)
                                                                                                           aTTTTCATACCCTGTCGTTTTTTTTCGATTTCAAAATA‑TACTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                                            <  2:5356728/150‑1 (MQ=255)
                                                                                                            ttttCATATCCTGTCGTGTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGc                                           >  2:6617247/1‑150 (MQ=255)
                                                                                                             tttCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGCTAGGGAAGCCGTAGCa                                          >  2:2263378/1‑150 (MQ=255)
                                                                                                             tttCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATAGCGATAACGATCACAAAAAAGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCa                                          >  2:3599942/1‑150 (MQ=255)
                                                                                                               tCATATCCTGTCGTTTGTTTTCTATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                        <  2:2875813/150‑1 (MQ=255)
                                                                                                                  tatCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                                     >  2:3792861/1‑150 (MQ=255)
                                                                                                                  tatCCTGTCGTTTGTTTTAGATTTCAAAATA‑TAATGAAAATATTTGTTTTTAAAAATAGAGATAACGATCACAAAAAAGAAAAAATTAAAATTATTAGAGGAGTGAAAG‑GC‑AAAAAAAAGGCCACCCGATAGGGAAGCCGTAGCAAAGTg                                     >  2:4124910/1‑150 (MQ=255)
                                                                                                                       tGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                                <  2:3325276/150‑1 (MQ=255)
                                                                                                                         tcttttgtttTCTATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                              <  1:4322585/147‑1 (MQ=255)
                                                                                                                           gtttgtttTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                            <  1:2067079/150‑1 (MQ=255)
                                                                                                                            tttgtttTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                           >  2:2407192/1‑150 (MQ=255)
                                                                                                                              tgtttTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa                         >  2:5229660/1‑150 (MQ=255)
                                                                                                                               tttttCGTTTTAAAAATA‑TAATTAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGTAAG‑GC‑AAAAAAAAGGCCTCCCGATAGGGTAGCCGTATCAAAGTGCGCGTGTTTTTAt                        <  1:184838/149‑1 (MQ=255)
                                                                                                                               gtttTAGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGAGCGTGTTTTTAt                        <  2:4592263/150‑1 (MQ=255)
                                                                                                                                    cGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg                   >  1:3336090/1‑150 (MQ=255)
                                                                                                                                    cGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg                   >  2:539041/1‑150 (MQ=255)
                                                                                                                                    cGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTAGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg                   >  1:104930/1‑150 (MQ=255)
                                                                                                                                     gATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGcc                                                <  2:1266561/120‑1 (MQ=255)
                                                                                                                                     gATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                  >  2:1609235/1‑150 (MQ=255)
                                                                                                                                       tttCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg                >  2:2209430/1‑150 (MQ=255)
                                                                                                                                       tttCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGACCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg                >  1:352511/1‑150 (MQ=255)
                                                                                                                                        ttCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc               >  2:5696034/1‑150 (MQ=255)
                                                                                                                                        ttCAAAATA‑TAAAGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc               >  2:2364121/1‑150 (MQ=255)
                                                                                                                                         tCAAAATA‑TAATTAATTTATTTGTTTTAAAATATCGAGATAACGATCACAACAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg              <  1:1707737/150‑1 (MQ=255)
                                                                                                                                            aaaTA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg           >  1:2590124/1‑150 (MQ=255)
                                                                                                                                            aaaTA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg           >  1:6136115/1‑150 (MQ=255)
                                                                                                                                            aaaTA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg           >  2:2138617/1‑150 (MQ=255)
                                                                                                                                             aaTA‑TATTTAATTTATTTTTTTTTAAATATCTATATAACGACAAAAAAAACGACAATATGAAAATTATTCGTGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt          <  1:5909728/150‑1 (MQ=255)
                                                                                                                                              ata‑tAATTAATTTTTTTTTTTTTAAATATAGATATAAAGATCACAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGTCCTCCCGATAGGGAAGCCTTATCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa         <  1:5864428/150‑1 (MQ=255)
                                                                                                                                               ta‑taATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGTGGAGTGTAAG‑GC‑AATATAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa        >  2:4271727/1‑150 (MQ=255)
                                                                                                                                               ta‑taATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCGCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa        <  2:5962269/150‑1 (MQ=255)
                                                                                                                                                a‑taATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAACAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa       <  1:1785471/150‑1 (MQ=255)
                                                                                                                                                  taATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc      <  1:4279976/150‑1 (MQ=255)
                                                                                                                                                  taATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc      <  1:3664467/150‑1 (MQ=255)
                                                                                                                                                     tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa   >  2:5026108/1‑150 (MQ=255)
                                                                                                                                                      gAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGACGCCGTAGCAAAGTGCGCGTGTTGTTATGCCGGATGCGGTGTAAACATAc  >  1:960701/1‑150 (MQ=255)
                                                                                                                                                      gAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc  <  1:4418323/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATA‑TAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAG‑GC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  NC_000913/2177096‑2177394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑