Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,267 C→A 8.4% intergenic (‑63/+245) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2670CA 8.4% 1014.0 / 14.0 331intergenic (‑63/+245)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (113/175);  new base A (40/2);  total (154/177)
Fisher's exact test for biased strand distribution p-value = 9.66e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 334 total aligned reads displayed.

TTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCAT  >  NC_000913/2177118‑2177415
                                                                                                                                                     |                                                                                                                                                      
ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                                                        <  1:1641489/150‑1 (MQ=255)
ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATGAAATAATTCGTTTTTAAATATc                                                                                                                                                        >  1:5965995/1‑150 (MQ=255)
 tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCCCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                                                       <  2:6542607/150‑1 (MQ=255)
   gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgag                                                                                                                                                     >  1:4869673/1‑150 (MQ=255)
       ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATaa                                                                                                                                                 >  2:3369919/1‑150 (MQ=255)
       ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCCGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATaa                                                                                                                                                 <  2:2139090/150‑1 (MQ=255)
           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATGTCGGGTTAACGAt                                                                                                                                             >  2:5461073/1‑150 (MQ=255)
           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                                                             <  2:5422556/150‑1 (MQ=255)
           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATGAAATTATTTTTTTTTAAATATCGAGATAAAGAt                                                                                                                                             >  2:2547095/1‑150 (MQ=255)
             atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                                                           <  1:6437767/150‑1 (MQ=255)
             atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTAAATTATTTTTTTTTAAATATAGAGATAACGAtaa                                                                                                                                           >  1:5033870/1‑148 (MQ=255)
                ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATAGAGATAACGAtaaaaa                                                                                                                                        >  2:4622075/1‑145 (MQ=255)
                   aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATAGAGATAACGATAACaaaaa                                                                                                                                     >  1:2718389/1‑150 (MQ=255)
                      gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                                                  <  2:763995/150‑1 (MQ=255)
                      gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTAAATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                                                  <  2:2301908/150‑1 (MQ=255)
                      gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTACAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                                                  <  2:794206/150‑1 (MQ=255)
                               gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACCATCAAAAAAAAGACAAAATGaa                                                                                                                         >  2:3194693/1‑150 (MQ=255)
                                 aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAAAGATCACAAAAACGCCAATATGacaa                                                                                                                       >  2:4171768/1‑150 (MQ=255)
                                       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTc                                                                                                                 <  2:3177679/150‑1 (MQ=255)
                                       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGATCACAAAAAAGACAAAATGAAAattatta                                                                                                                 >  2:5909728/1‑149 (MQ=255)
                                        ccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAAAAATTAAAATATTTTTTTTTAAATATATAGATAAAGATAAAAAAAAAAACAAAAAGAAAaatattag                                                                                                                >  1:2872145/1‑148 (MQ=255)
                                         cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                                                               <  2:1289720/150‑1 (MQ=255)
                                             cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAAATAAATTATTTTTTTTTAAATATAGAGATAAAGATAACAAAAAAAAAAAAATTAAAAATATTAgaggag                                                                                                           >  1:3456377/1‑150 (MQ=255)
                                                tgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAATATAATGTAATTTTTTTTTTTTAAATATAGATAAAAAGATCACAAAAAAGAAAATATTTAAAATATTAGAGGGGTGa                                                                                                        >  1:2181750/1‑150 (MQ=255)
                                                  cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                                                      >  2:5544127/1‑150 (MQ=255)
                                                     tgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGc                                                                                                  <  2:4098901/150‑1 (MQ=255)
                                                      gttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCa                                                                                                <  2:5375127/150‑1 (MQ=255)
                                                         cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATAAAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAAAGAAAATATTTAAAATATTAGAGGAGTGAAAGGCaaaa                                                                                             >  2:2740702/1‑150 (MQ=255)
                                                            cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAAATTAAATATTTGTTTTTTTATATAGAGATAACGAAAACAAAAAACACAAAATTTAAAATAATAGAGGAGTGAAAGGCccaaaaa                                                                                          >  1:409406/1‑150 (MQ=255)
                                                             aTCTGCTTTGTCGGTACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGTATTAAAAAAATAATTAAAATATTTTTTTTTAAATAAAGAGATAACGAAAACAAAAAACACAAAAATAAAAATATTAGAGGAGTGTAAGTGCaaaaaaa                                                                                          >  1:1098597/1‑150 (MQ=255)
                                                             aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGATTGGTTTCGATTTAAAAAAATAAAGAAATTATTTGTTTTTAAATATAGAGATAACGAACACAAAAAAGACCAAATGAAAATTATTAGAGGAGGGGAAGGCAAAAAAAc                                                                                         >  2:6224288/1‑150 (MQ=255)
                                                             aTCTGCTTTGTCGATACAACTTAATTTTTAATATCCTGTCGTTTTTTTTATATTTAAAAATATAATGAAATTTTTTTTTTTTAATTATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                                         <  1:426289/150‑1 (MQ=255)
                                                             aTCTGCTTGGTCGATACCACGTACTTTTTCATAGCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                                         <  2:1351768/150‑1 (MQ=255)
                                                                tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAAATATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGc                                                                                      >  2:5318248/1‑150 (MQ=255)
                                                                  cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAAAGATAACAAAAAAGACAAAATGAAAATTATTAGAGGAGTGAAAGGCAAAAAAAAGGcca                                                                                    >  2:1975078/1‑149 (MQ=255)
                                                                  cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAAAGACAATATGCAAATTATTAGAGGAGTGAAAGGCACAAAAACGGcca                                                                                    >  2:4034442/1‑149 (MQ=255)
                                                                         gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAACATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                                                             >  2:4447568/1‑150 (MQ=255)
                                                                         gATACCACCTACATTTTAATATCCTGTCTTTTGTTTTCGATTTAAAAATATAATTAAATTATTTGTTTTTAAATATCTAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                                                             <  2:11711/150‑1 (MQ=255)
                                                                            aCCCCGAACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAggg                                                                          <  2:4314553/150‑1 (MQ=255)
                                                                            aCCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTTAATATCGAGATAACGATCACAAAAACGACAAAATGAAAATTATTCGAGGAGTGTAAGGCAAAAAAAAGGCCTCCCCAGAggg                                                                          >  2:678158/1‑150 (MQ=255)
                                                                             ccACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTCAAAAAATAAAGAAATTATTTTTTTTTAAATATACAGATAAAGATCACAAAAAAGACAATATGAAAATTATTCGAGGTGTGAAAGGCAAAAAAAAGGCCGCCCGAGAgggg                                                                         >  2:2943968/1‑149 (MQ=255)
                                                                              cACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                                                        <  2:1561964/150‑1 (MQ=255)
                                                                               aCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                                                       <  2:1689460/150‑1 (MQ=255)
                                                                                 ttaatttttAATATCCTTTGTTTTTTTTTCTTTTAAAAAATATAATTATATTTTTTTTTTTTAATTTTCTATATAACTAAAACAAAAACGAAAATATTAAAATTATTCGAGGAGTGAAAGGAAAAAAAACGGCCTCCCGATAGGGAAGcc                                                                     <  1:2575469/145‑1 (MQ=255)
                                                                                   actttttCATATCCTGTGTTTTGTTTTCGATTAAAAAATATAATCAACTTATTTTTTTTTAAATATCTAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCGt                                                                   <  1:6059408/147‑1 (MQ=255)
                                                                                   aCATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCGt                                                                   <  2:6093648/150‑1 (MQ=255)
                                                                                    aaTTTTCAAAGCGGGTGGTTTTTTTGCGATTTAAAAAAATAATTAATTTTTTTTTTTATAAATTTCGACAAAACTAAAAAAAAAACGACAATATGAAAATTATTCGAGGTGTTAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                                                  <  1:3131950/149‑1 (MQ=255)
                                                                                     aTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                                                  <  2:3483517/150‑1 (MQ=255)
                                                                                     aTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAATTATTTGTTTTTAAATAACGAGAAAACCATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                                                                 >  2:3755827/1‑150 (MQ=255)
                                                                                         tCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                                             <  2:2875813/150‑1 (MQ=255)
                                                                                             atCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAATTTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgc                                                         <  1:4308749/150‑1 (MQ=255)
                                                                                             atCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgc                                                         <  2:2260114/150‑1 (MQ=255)
                                                                                                 tGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                                                     <  2:3325276/150‑1 (MQ=255)
                                                                                                   tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                                                   >  2:5779712/1‑150 (MQ=255)
                                                                                                     gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                                                 <  1:2067079/150‑1 (MQ=255)
                                                                                                      tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                                                >  1:5866766/1‑150 (MQ=255)
                                                                                                      tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                                                >  1:1922113/1‑150 (MQ=255)
                                                                                                        tgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa                                              >  2:5229660/1‑150 (MQ=255)
                                                                                                         gtttTCTATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                                             <  1:184471/150‑1 (MQ=255)
                                                                                                          ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                                            >  2:5079160/1‑150 (MQ=255)
                                                                                                          ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                                            >  1:6358015/1‑150 (MQ=255)
                                                                                                              cGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg                                        >  1:3336090/1‑150 (MQ=255)
                                                                                                              cGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTAGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg                                        >  1:104930/1‑150 (MQ=255)
                                                                                                               gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                                                     >  1:1266561/1‑120 (MQ=255)
                                                                                                               gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                                       >  2:6647246/1‑150 (MQ=255)
                                                                                                                 tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg                                     >  2:2209430/1‑150 (MQ=255)
                                                                                                                 tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGACCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg                                     >  1:352511/1‑150 (MQ=255)
                                                                                                                  ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAATATTATTCGTGGAGTGAAAGGGAATATTACGGCCTCCCGATAGGGAAGCCGTAGCAATGTGGGCGTGTTTTTATGGCGGATGc                                    >  2:2363859/1‑150 (MQ=255)
                                                                                                                  ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc                                    >  2:5695893/1‑150 (MQ=255)
                                                                                                                   tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                   >  2:6390328/1‑150 (MQ=255)
                                                                                                                   acAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                   <  1:5910011/149‑1 (MQ=255)
                                                                                                                      aaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGTATATTATTCGAGGAGTGAAAGGCAGAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg                                >  2:4892791/1‑150 (MQ=255)
                                                                                                                      aaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg                                >  2:2402088/1‑150 (MQ=255)
                                                                                                                       aaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt                               <  1:3826286/150‑1 (MQ=255)
                                                                                                                           taATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc                           <  1:3664467/150‑1 (MQ=255)
                                                                                                                              tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa                        >  2:411755/1‑150 (MQ=255)
                                                                                                                              tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa                        >  1:1713043/1‑150 (MQ=255)
                                                                                                                               gAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGACGCCGTAGCAAAGTGCGCGTGTTGTTATGCCGGATGCGGTGTAAACATAc                       >  1:960701/1‑150 (MQ=255)
                                                                                                                               gAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc                       <  1:4418323/150‑1 (MQ=255)
                                                                                                                                aaaTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACg                      >  2:3837023/1‑150 (MQ=255)
                                                                                                                                aaaTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACg                      >  1:5606289/1‑150 (MQ=255)
                                                                                                                                   ttattTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTgg                   >  1:1509777/1‑150 (MQ=255)
                                                                                                                                     attTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCa                 <  1:6617247/150‑1 (MQ=255)
                                                                                                                                      tttGTTTTTAAATATCGAGATAACGATCACAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGt               <  1:2475397/150‑1 (MQ=255)
                                                                                                                                       ttGTTTTTAAATATCGAGATAGCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCACAGTGCGCGTGTTTTTATGCCGGATGCGGTGTACACATACGTGGCAgg               >  1:1791744/1‑149 (MQ=255)
                                                                                                                                        tGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTg              <  2:3985099/150‑1 (MQ=255)
                                                                                                                                         gTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGc             >  1:5445351/1‑150 (MQ=255)
                                                                                                                                         gTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGc             <  1:2991402/150‑1 (MQ=255)
                                                                                                                                         gTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGc             >  2:4755659/1‑150 (MQ=255)
                                                                                                                                         gTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGc             >  2:4246779/1‑150 (MQ=255)
                                                                                                                                           ttttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTa           >  1:843686/1‑150 (MQ=255)
                                                                                                                                           ttttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTa           >  1:1331838/1‑150 (MQ=255)
                                                                                                                                            tttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTAt          <  2:2764649/150‑1 (MQ=255)
                                                                                                                                              tAAATATCTATATAACGATCTCAAAAACGACAATATGAAAATTATTCGAGGAGTGTATGGCAAAAAAACGGCCTCCCGATAGGGTAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGt        <  1:6224288/150‑1 (MQ=255)
                                                                                                                                              tAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGt        <  1:5646975/150‑1 (MQ=255)
                                                                                                                                               aaaTATCGAGATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTa       <  1:719940/150‑1 (MQ=255)
                                                                                                                                               aaaTATCGAGATAACGATCACAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGTAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTa       >  2:5032437/1‑150 (MQ=255)
                                                                                                                                               aaaTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTa       >  1:2388655/1‑150 (MQ=255)
                                                                                                                                                  tatCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGc    <  2:1096609/150‑1 (MQ=255)
                                                                                                                                                    tCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGt                                                                                                          <  2:1856540/48‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCAT  >  NC_000913/2177118‑2177415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: