Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,229,177 A→C 14.7% intergenic (+112/+261) yohP → / ← dusC uncharacterized protein/tRNA‑dihydrouridine synthase C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,229,1770AC14.7% 380.9 / 7.5 211intergenic (+112/+261)yohP/dusCuncharacterized protein/tRNA‑dihydrouridine synthase C
Reads supporting (aligned to +/- strand):  ref base A (46/104);  new base C (23/11);  total (77/134)
Fisher's exact test for biased strand distribution p-value = 1.28e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 217 total aligned reads displayed.

TACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATG  >  NC_000913/2229028‑2229326
                                                                                                                                                      |                                                                                                                                                     
tACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCa                                                                                                                                                       >  1:2669825/1‑150 (MQ=255)
   tggtggTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAggg                                                                                                                                                    <  2:549701/150‑1 (MQ=255)
   tggtggTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAggg                                                                                                                                                    >  1:3136452/1‑150 (MQ=255)
   tggtggTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAggg                                                                                                                                                    <  2:1239539/150‑1 (MQ=255)
     gtggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCggggg                                                                                                                                                  >  1:1985932/1‑150 (MQ=255)
     gtggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAggggg                                                                                                                                                  >  2:499089/1‑150 (MQ=255)
      tggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGggc                                                                                                                                                 >  2:3152879/1‑150 (MQ=255)
      tggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAgggggg                                                                                                                                                 >  1:448760/1‑149 (MQ=255)
      tggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggc                                                                                                                                                 <  2:4719052/150‑1 (MQ=255)
       ggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcg                                                                                                                                                <  1:2495170/150‑1 (MQ=255)
        gtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGggcgg                                                                                                                                               >  1:6134110/1‑150 (MQ=255)
        gtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcgg                                                                                                                                               <  2:1740856/150‑1 (MQ=255)
        gtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcgg                                                                                                                                               <  1:1083343/150‑1 (MQ=255)
           aCTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCAGCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGggggggggggggg                                                                                                                                            >  1:2279941/1‑147 (MQ=255)
             tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGgggggggggggccca                                                                                                                                          >  1:1847319/1‑145 (MQ=255)
             tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCGGGGGGGGGcccaa                                                                                                                                          >  1:3090394/1‑147 (MQ=255)
             tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCaaa                                                                                                                                          >  2:1845373/1‑149 (MQ=255)
               gCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGTATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGggcgggccatag                                                                                                                                        >  1:5536244/1‑143 (MQ=255)
                 gTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCaaaagcc                                                                                                                                      >  2:4681249/1‑145 (MQ=255)
                 gTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgcc                                                                                                                                      <  2:2161654/150‑1 (MQ=255)
                 gTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAAcgcc                                                                                                                                      >  2:3956520/1‑150 (MQ=255)
                    tttAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATagccgcc                                                                                                                                   >  2:5457325/1‑150 (MQ=255)
                      tAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgccgccgc                                                                                                                                 <  2:3254617/150‑1 (MQ=255)
                       aaGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCgccgccccc                                                                                                                                >  2:6688007/1‑150 (MQ=255)
                       aaGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGCCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCgccgccgcc                                                                                                                                <  2:6556573/150‑1 (MQ=255)
                        aGATGATATTTTAAAATTAATTAATGTCATCAGGTCCTAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGccc                                                                                                                               <  1:587459/150‑1 (MQ=255)
                         gatgatATTTTAAAATTAATTACTGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCAGATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGcccc                                                                                                                              <  2:2534452/150‑1 (MQ=255)
                             atatTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGGCGGGGGGGGCCAAACcccccccccccccc                                                                                                                           >  1:6224436/1‑148 (MQ=255)
                             atatTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCAAACGCCGCCCCCCCCCGc                                                                                                                          >  1:6692225/1‑150 (MQ=255)
                              tatTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCCGCCAAACCCCCCCCCCCCCAGcc                                                                                                                         >  1:3856141/1‑149 (MQ=38)
                                ttttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAACGCCGCCCCCCCCAGcccc                                                                                                                       >  2:5914839/1‑150 (MQ=255)
                                 tttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCGCTGGCGCAAGGAGCGCGCCGGGGGCGGCCCAACccccccccccccccccccc                                                                                                                      >  1:6510084/1‑143 (MQ=255)
                                 tttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCAAAAGCCCCCCCCCCCAGCAccc                                                                                                                      >  2:3310331/1‑150 (MQ=38)
                                  ttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGGGGGGGCGGCCAAAACCCGCCCCCCCCAGCAcccc                                                                                                                     >  1:3761697/1‑150 (MQ=255)
                                    aaaaTTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGGGGGGGGGGGGGATAGGCGGCGGCCCCAGCGcccccc                                                                                                                   >  1:1322646/1‑149 (MQ=255)
                                    aaaaTTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCg                                                                                                                   <  2:53879/150‑1 (MQ=255)
                                     aaaTTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCAAACCCGCCCCCCCCACCAccccccc                                                                                                                  >  2:4104558/1‑148 (MQ=38)
                                     aaaTTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAACACCCCCCCCCCCGGCAccccccc                                                                                                                  >  2:3570480/1‑148 (MQ=38)
                                       attaattaATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggc                                                                                                                                                 <  2:1235384/117‑1 (MQ=255)
                                        ttaattaaTGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCCGGGGCGGCCCAAACCCCCCCCCCCCACCAcccccccgcg                                                                                                               >  1:2154800/1‑145 (MQ=255)
                                          aattaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGctct                                                                                                             <  2:4930164/150‑1 (MQ=255)
                                            ttaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTgg                                                                                                           <  2:2115779/150‑1 (MQ=255)
                                            ttaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCCGGcgcagg                                                                                                           >  2:5428606/1‑145 (MQ=255)
                                                  tcatcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACa                                                                                                     <  2:2371719/150‑1 (MQ=255)
                                                     tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCATCCCCCCCCCCCCCCGCCCCCCCCGGCTCAGGCGAACaaaa                                                                                                  >  2:5590697/1‑150 (MQ=25)
                                                     tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCACTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACaaaa                                                                                                  <  2:5814367/150‑1 (MQ=255)
                                                     tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACaaaa                                                                                                  <  2:4045449/150‑1 (MQ=255)
                                                       aGGTCCGAAAATAACGAGAATATTTCAGCCGCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATCTCATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATc                                                                                                <  2:2647079/150‑1 (MQ=255)
                                                        ggTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcg                                                                                               <  2:1122235/150‑1 (MQ=255)
                                                         gTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGTGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcgc                                                                                              <  2:4281717/150‑1 (MQ=255)
                                                          tCCGAAAATAACGAGAATATTTCTGTCTCTCATCCTGTTGCGCTCCTGTCTTGTGCATTGCTTCATATAATCACTGGCGCAAGGTGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcgcc                                                                                             <  2:3472377/150‑1 (MQ=255)
                                                            cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGGCCCACCCCCCCCCCCCCACCACCCCCCCGCCCAGGCCGACaaaaaaacccc                                                                                           >  1:5499659/1‑143 (MQ=255)
                                                                        taaTTTTTCTGTCTCTAATCCTTTTGCGCTCCTGTATTGTGCTTTGGTTAATATAATCACGGGCGCATGGAGCGCGCGGGGGGGGGCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGccc                                                                               <  1:495896/149‑1 (MQ=17)
                                                                             tttCAGTCTCTCATCCTGTTTCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCTGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc                                                                          <  2:5376569/150‑1 (MQ=255)
                                                                                 aGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAt                                                                      <  2:5679289/150‑1 (MQ=255)
                                                                                 aGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAt                                                                      <  2:4331334/150‑1 (MQ=255)
                                                                                  gTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATc                                                                     <  2:6324972/150‑1 (MQ=255)
                                                                                   tctctcAGCCTGTTGCGCTCCTGTCTTGTGCTTTGCTACATATAATCACGGGCGCACGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATcc                                                                    <  1:3373352/150‑1 (MQ=17)
                                                                                     tctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAAAGCCCCCGCCCCCAGCACCCCCCGGCGCTGGCGAACAAAAAAGCCCCAGCGCGGTGCCCGCGGCGTATacca                                                                  >  2:2825794/1‑149 (MQ=18)
                                                                                     tctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCACGGGGCGGCCAAAACCCCCCCCCCCCCCCCCCCCCCCGCGCGGGCGCACAAAAAACCCCCGGCGCCGTGGCCCCCGCGTATccca                                                                  >  2:5949412/1‑149 (MQ=16)
                                                                                     tctcATCCTGTGGCGCTCCTGTCTTGTGCTTTGCTACATATAATCCCTGGCGCAAGGAGCGCGCTGGGGGCGGCAAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCt                                                                  <  1:2151505/150‑1 (MQ=17)
                                                                                     tctcACCCTGTTGCGCTCCTTTCTGGTGCTTTGATACATATAAACACTGGCGCATGGTGCGCGCCGGGGGCGGCAATCCGCCGCCGCCCCGTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCt                                                                  <  1:1716259/150‑1 (MQ=14)
                                                                                       tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTa                                                                <  2:4241268/150‑1 (MQ=255)
                                                                                       tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCCGCCAAAAGCCGCCCCCCCCAGCACCCCCCGGCACATGCGAAAAAAAAAGCCCCAGCGCGGTGCCCACCGCTTATCCCGTa                                                                >  2:2234043/1‑150 (MQ=18)
                                                                                             tGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCCCCCCCGGCACCCCCCGGCGCTGGCGAACAAAATCGCCGCGGCGCGGGGGCCGCGGCGTATCCCGTACGGCTa                                                          >  2:6130935/1‑150 (MQ=17)
                                                                                                tGCGCTCCTGTCATGTGAATTGCTTCATATAATCCCTGGCGCATGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCg                                                       <  1:5745130/150‑1 (MQ=255)
                                                                                                   gcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGt                                                    <  1:5839263/150‑1 (MQ=255)
                                                                                                   gcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGt                                                    <  1:4884096/150‑1 (MQ=255)
                                                                                                    cTCCTGTCATGTGCATTGCTTCATATAATCCGTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTc                                                   <  1:3245722/150‑1 (MQ=255)
                                                                                                      ccTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCgg                                                 <  2:1943368/150‑1 (MQ=255)
                                                                                                      ccTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCgg                                                 <  1:2779012/150‑1 (MQ=255)
                                                                                                      ccTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCgg                                                 <  2:3368440/150‑1 (MQ=255)
                                                                                                        tGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAACGCCGCCCCCCCCAGCACCCCCCGGCTCTGGCGAACAAAATCGCCGCGGCGCGGTGCCCGCGGCTTATCCCATACGGCTACCCGGTCGGgc                                               >  2:3777054/1‑150 (MQ=17)
                                                                                                            aTGTGCTTTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGa                                           <  1:1293587/150‑1 (MQ=255)
                                                                                                            aTGTGCATTGCTTCATATAATCACTGGCGCATGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGa                                           <  1:4104558/150‑1 (MQ=255)
                                                                                                            aTGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGa                                           <  1:150531/150‑1 (MQ=255)
                                                                                                             tgtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAg                                          <  2:2685907/150‑1 (MQ=255)
                                                                                                                gCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTa                                       <  2:4077589/150‑1 (MQ=255)
                                                                                                                 cATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGAGGGGGGGGCCCAAAGCCCCCCCCCCCAGCCCCCCCCGGCGCAGGCGGACAAAAAAACCGCCGCGCGGGGGCCCCCGCGTATACCCAAACGCGAACCGGTCGGGCGCGCGGGaa                                      >  2:6422828/1‑150 (MQ=9)
                                                                                                                   ttGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                    <  1:4187646/150‑1 (MQ=255)
                                                                                                                      cTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCCATCGCCCCCCCCCCCAGCACCCCCCGGCACAGGCGAACAAAAAACCCCCGGCGCGGTGCCCACCGCGTATACCATAAGGCTACCCGGTCCGGCGCGAGGTAACAtac                                 >  2:3751211/1‑148 (MQ=9)
                                                                                                                      cTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAAAGCCGCCCCCCCCAGCACCCCCCCGCGCAGGCGAACAAAAACGCCCCTGCGCGGTGCCCACGGCGTATACCAAACCGCTACCCGGTCCGGCGCGCGGTAACAAcc                                 >  2:3630882/1‑150 (MQ=9)
                                                                                                                        tCATATAATCACTGGCGCATGGAGCGCGCAGGGGGCGGCAAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCTAACAAACTCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCt                               <  1:122211/150‑1 (MQ=255)
                                                                                                                        tCATATAATCACTGGCGCAAGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACTTCCCt                               <  1:4262873/150‑1 (MQ=255)
                                                                                                                        tCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCt                               <  2:4005135/150‑1 (MQ=39)
                                                                                                                        tCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCt                               <  2:3814881/150‑1 (MQ=39)
                                                                                                                              aaTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTaaaa                         <  1:1382790/150‑1 (MQ=34)
                                                                                                                              aaTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTaaaa                         <  1:5428606/150‑1 (MQ=34)
                                                                                                                               aTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAc                        <  1:5399645/150‑1 (MQ=33)
                                                                                                                                  aCTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgcg                     <  2:1847542/150‑1 (MQ=32)
                                                                                                                                   cTGTCGCAAGGAGCGCGCAGGGGGCGGCCACTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACgcgc                    <  1:2796465/150‑1 (MQ=17)
                                                                                                                                   cTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACgcgc                    <  1:767897/150‑1 (MQ=32)
                                                                                                                                   cTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACgcgc                    <  1:3780156/150‑1 (MQ=32)
                                                                                                                                   cTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACgcgc                    <  2:1322646/150‑1 (MQ=32)
                                                                                                                                   cTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCACCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACgcgc                    <  2:3803508/150‑1 (MQ=17)
                                                                                                                                    tGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGcc                   <  2:1443967/150‑1 (MQ=32)
                                                                                                                                           aGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc            <  2:4138075/150‑1 (MQ=32)
                                                                                                                                                     cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAt   <  1:2825794/150‑1 (MQ=32)
                                                                                                                                                     cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAt   <  2:4577098/150‑1 (MQ=32)
                                                                                                                                                      aGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtg  >  2:2585451/1‑150 (MQ=32)
                                                                                                                                                      |                                                                                                                                                     
TACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATG  >  NC_000913/2229028‑2229326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: