Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,431 G→T 10.1% R434I (AGA→ATA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4310GT10.1% 754.7 / 4.4 256R434I (AGA→ATA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base G (91/126);  new base T (21/5);  total (125/131)
Fisher's exact test for biased strand distribution p-value = 2.50e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 264 total aligned reads displayed.

TATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGA  >  NC_000913/2470284‑2470580
                                                                                                                                                   |                                                                                                                                                     
taTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGaa                                                                                                                                                     <  1:5195025/150‑1 (MQ=255)
  tctcTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAt                                                                                                                                                   <  2:3850876/150‑1 (MQ=255)
   ctctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATc                                                                                                                                                  <  1:6421133/150‑1 (MQ=255)
     ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                                                                                                                <  1:2922567/150‑1 (MQ=255)
     ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                                                                                                                >  1:2686907/1‑150 (MQ=255)
      tttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTAATTATAAAAAAAataaaataaaaaaat                                                                                                                                               >  1:4523332/1‑145 (MQ=255)
      tttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAtaaataaat                                                                                                                                               >  1:4315046/1‑149 (MQ=255)
      tttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAAATCAAAGAAATCaat                                                                                                                                               >  1:2822611/1‑149 (MQ=255)
            gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                                                                                                         <  2:4334344/150‑1 (MQ=255)
            gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATCAAGaaaaaa                                                                                                                                         >  2:4075568/1‑148 (MQ=255)
            gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAAAAAATa                                                                                                                                         >  2:690747/1‑150 (MQ=255)
             aaTATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAg                                                                                                                                        <  2:2587328/150‑1 (MQ=255)
                 tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                    <  2:6469329/150‑1 (MQ=255)
                   tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAATCAAGACAATAGCGAttt                                                                                                                                  >  2:6089900/1‑148 (MQ=255)
                    cAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAAAGCGATGTa                                                                                                                                 >  2:3750330/1‑150 (MQ=255)
                      aTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTaaa                                                                                                                               <  1:1899488/150‑1 (MQ=255)
                       ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                              <  1:2049335/150‑1 (MQ=255)
                       ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                              <  2:1589135/150‑1 (MQ=255)
                              tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAAAAAATAAAAAAAAAAAAAACAAAAACAATGTAAAGAACTaaa                                                                                                                       >  1:746602/1‑149 (MQ=255)
                                tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAACAAGACAAAAGCGATGTAAAGCACTAAggt                                                                                                                     >  2:1609149/1‑148 (MQ=255)
                                  aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAAAAATTAAAATAAATAAAGTAAAAAGAGAAGGAAATCCATAAGTTTa                                                                                                                   >  1:4553710/1‑150 (MQ=255)
                                   tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaa                                                                                                                  <  2:2812776/150‑1 (MQ=255)
                                    ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGa                                                                                                                                              <  1:985356/121‑1 (MQ=255)
                                     tttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAAt                                                                                                                <  2:4863819/150‑1 (MQ=255)
                                      ttCGGGGTAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAAtt                                                                                                               <  1:4266108/150‑1 (MQ=255)
                                      ttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAAtt                                                                                                               <  1:2225383/150‑1 (MQ=255)
                                       tCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTg                                                                                                              <  2:2082413/150‑1 (MQ=255)
                                         ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAAATAAAAGAAAAAAAGACAAAAGCGATGTAAATCACAAAGTTTAAATTtcg                                                                                                            >  2:3152986/1‑150 (MQ=255)
                                           ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAAAAACGATGTAAATCACTAAGTTTAAATTtcgcg                                                                                                          >  1:1092223/1‑150 (MQ=255)
                                            gAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAAGAAAAAAAAAAAAAATCGATGTAAAGCAATAAAATTTAATTtagcgc                                                                                                         >  1:4909028/1‑150 (MQ=255)
                                             aaGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGcc                                                                                                        <  2:5205704/150‑1 (MQ=255)
                                               ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAAAACAAAAAAGATGTAAAGCACAAATGTTAAATTTAGCGccga                                                                                                      >  1:6549262/1‑148 (MQ=255)
                                                     agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTTTAAAATAAATAAAATAAATAAAGAAAAAAGAGATGTAAAGAACAAACTTTAAAATTGGCGCCAAtaaagg                                                                                                >  1:4048900/1‑145 (MQ=255)
                                                      gagaTTTAATCAATCATTTATTTTGAATTTATTTTTCGTTCGACCTTTGATTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc                                                                                               <  2:4272151/150‑1 (MQ=255)
                                                      gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc                                                                                               <  1:438997/150‑1 (MQ=255)
                                                      gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAATAAAACAAGAAAAAATCGATGTAAAGCACAAAGTTTTAATTTCGCGCCCAACAAGgc                                                                                               >  2:2995391/1‑150 (MQ=255)
                                                       agaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTTTTAAAAAAATAAAAAAAAAAAAAAAAAAAAAGAGTGAAAGCCCAAATTTTAAAATGCGCGCCAATccagggg                                                                                              >  1:5322992/1‑144 (MQ=255)
                                                           ttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTGAAACAAATAAAGACAATATCGATTTAAAGCCCTAAGTTTAAATTTCGCGCCAAACATGgcgcgcc                                                                                          >  2:5906588/1‑149 (MQ=255)
                                                           ttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGca                                                                                          <  2:419601/150‑1 (MQ=255)
                                                                caagcaTTTATTTTGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTAAAAAAAAATTCAAAGAAAAAAAAACAAAAGCGATGTAAAGCACTAAATTTAAAATGAGCGCCAATCAAAGCGCCCaaaaac                                                                                     >  2:5919549/1‑148 (MQ=255)
                                                                caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATTAAAAAATTAAAAAAAAAAAAAAAAAAATAGATGTTAAGAAAGAAATTTTAATTTCTCGCCAAACAAGTCGCGCCCAAGc                                                                                     >  1:5038209/1‑150 (MQ=255)
                                                                caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGc                                                                                     <  2:4389127/150‑1 (MQ=255)
                                                                 aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTCAAAGAAATAAAGACAAAATCGATGTAAAGCAAAAAGTTTAAATTGCGCGCCCATCATGGCCCGCACAAGca                                                                                    >  2:5851852/1‑149 (MQ=255)
                                                                 aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTATTTATTAAATAAATAAAAAAAAAAAAAAAAAAAACGATGTAAAGGACAAAGTTTAAAATTCGCGCCCAACAAGGCGCGCACAAGca                                                                                    >  1:5912050/1‑149 (MQ=255)
                                                                 aagcaTTTATTTGGAATGTATTTTTCGTTAGACCTTTGCTTATACATAACAGTCGTGTTTTTTAATTATAAAAAAAATAAAATAAAAAAAAACAAGAGAGAGTTAAATCACAAAGTTTTAATTTATCTCCAAAAAAGGCGCGCCCAAGca                                                                                    >  1:3419799/1‑149 (MQ=255)
                                                                   gcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCtat                                                                                  <  2:1255765/150‑1 (MQ=255)
                                                                     atttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataa                                                                                <  2:2552501/150‑1 (MQ=255)
                                                                       ttatttGTATTTTATTTTTCGTTCGACTTTTGTTTATAAATAACTGTGTTTTTTTTATTTAATAAAATAATACAAATAAATCAAGAAATTTGCTATTTAAAGAACTATTTTTAATTTGCGCCCAAATCTGGGCGCGCACAAGCTataata                                                                              <  1:576411/150‑1 (MQ=255)
                                                                          tttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCa                                                                           <  2:2351342/150‑1 (MQ=255)
                                                                           ttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAAAAAAAAAAAAAAGAATTAAAGAAATAAGATTAAATTGCGCGCCAAACAAGGCGCACACAAACAaaaataaaaa                                                                          >  2:1857253/1‑146 (MQ=255)
                                                                            tGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTCAAATAAAACAAGAAAAAAGCGATGTAAAACACTAAGTTTAAATTTCGCGCCCAACAATGCGCGCACAAGCTATAATAACaaa                                                                         >  2:1170802/1‑149 (MQ=255)
                                                                               tttttttttttCGTTCGACCTTTTTTTATAAATAAATGTATTTTTTTTAATTTATAAAATAATTAAAATAAATCAAGACATTCGGTTTTTAAAGAACTATTTTTAATTTGCGCGCAAATCTTGGCGCGCACAAGCTATAATACCAACCTa                                                                      <  1:114151/144‑1 (MQ=255)
                                                                                 tGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGTGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAt                                                                    <  2:1426118/150‑1 (MQ=255)
                                                                                 tGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAATAAAAAAAGAAAAAAACGATGTAAATCAATAAGTTTAAATTTAGCGCCAAGAAAGGCGCGCACAACCGATAATAACAAACAAAt                                                                    >  2:2300882/1‑150 (MQ=255)
                                                                                     tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAATGGGTAAGTTTAAATATCGCGCCCATCATGGGGCGCACAAGCTATAATACCAACCAACTTtct                                                                >  2:269910/1‑150 (MQ=255)
                                                                                     tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAAAATCGATGTAAATCACTAAGTTTAAATTTCGCGCCAATCCAGGCGCGCACAACCGATAATACCAACCAAATTtct                                                                >  2:5831658/1‑150 (MQ=255)
                                                                                      ttttCGTTCGACCTTTGCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                               <  1:3981495/150‑1 (MQ=255)
                                                                                      ttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                               <  2:4781058/150‑1 (MQ=255)
                                                                                            ttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACAAAAGCGATGTAAAGAACTAAGTTTAAATTGAGCGCCAATCATGGCGCGCACAAGCAAAAATACCAACCACATTTatccaccac                                                         >  2:4037407/1‑148 (MQ=255)
                                                                                             tcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATCAAGACAATAGCGATGTAAAGCACGAAGTTTAAATTTCGCGCCCATCATGGCGCGCACAAGCGATAATACCAACCTAATTTATCCACctct                                                        >  2:927331/1‑150 (MQ=255)
                                                                                               gACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATCCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTa                                                      <  2:570950/150‑1 (MQ=255)
                                                                                                   tttGATTATAAATATCAGTATTTTTTTTAATTTATATCATATTTAATTGATAACATCACAATAGCTATGTAAACCACTATGTTTAATGTGCGCTCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGt                                                  <  1:3151668/150‑1 (MQ=255)
                                                                                                   ttggCTTATACATAACAGTCGTTTTTTTAATGTATAAAATAATTCAAAGACATCAAGACAATCGCGATGTACAGCACTAAGTTGAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATCCCAACCTAATTTCTCCTCCTCTTCGAGt                                                  <  2:6006278/147‑1 (MQ=255)
                                                                                                      gCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAAATCGATGTAAAGCACGAAGTTTAAATTGCGCGCCAATCAAGGCGCGCCCCAGCGATAATACCAACCTAAATTCTACTCCTCTTAGAGTGAc                                               >  2:2622199/1‑150 (MQ=255)
                                                                                                           taCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCGATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTatat                                          <  2:6565674/150‑1 (MQ=255)
                                                                                                           taCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTatat                                          <  2:5048269/150‑1 (MQ=255)
                                                                                                           taCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTatat                                          <  2:2381532/150‑1 (MQ=255)
                                                                                                            aCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATTGCTATTTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtc                                         <  1:2300882/150‑1 (MQ=255)
                                                                                                             cATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtct                                        <  2:1947295/150‑1 (MQ=255)
                                                                                                               tAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTcc                                      <  1:2758821/150‑1 (MQ=255)
                                                                                                                   aGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGAt                                  <  1:702973/150‑1 (MQ=255)
                                                                                                                       gTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGAc                                               <  2:2284944/133‑1 (MQ=255)
                                                                                                                           ttttAATTTAAAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                          >  2:6343259/1‑150 (MQ=255)
                                                                                                                            tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                         <  2:336303/150‑1 (MQ=255)
                                                                                                                            tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                         >  2:4662109/1‑150 (MQ=255)
                                                                                                                            tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                         <  1:5799691/150‑1 (MQ=255)
                                                                                                                            tttAATTTATAAAATAATTAAAATAAATCAAGACAATAGCTATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                         <  1:2995391/150‑1 (MQ=255)
                                                                                                                             ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                        >  2:447230/1‑150 (MQ=255)
                                                                                                                                aTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAt                     >  1:4726004/1‑150 (MQ=255)
                                                                                                                                aTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAt                     >  1:1623307/1‑150 (MQ=255)
                                                                                                                                aTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAt                     >  1:4973500/1‑150 (MQ=255)
                                                                                                                                 tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAtt                    >  1:5550647/1‑150 (MQ=255)
                                                                                                                                   tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                  <  1:4807697/150‑1 (MQ=255)
                                                                                                                                   tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                  >  1:1001930/1‑150 (MQ=255)
                                                                                                                                     taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt                >  1:1574948/1‑150 (MQ=255)
                                                                                                                                     taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAGTGCGGTATTGACt                >  1:3465370/1‑150 (MQ=255)
                                                                                                                                       aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCTCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt              >  2:672265/1‑150 (MQ=255)
                                                                                                                                       aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt              >  1:6176895/1‑150 (MQ=255)
                                                                                                                                        aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGACTTGCGGTATTGACTATc             >  2:2752240/1‑150 (MQ=255)
                                                                                                                                        aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc             <  1:3628223/150‑1 (MQ=255)
                                                                                                                                           aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa          <  2:2026734/150‑1 (MQ=255)
                                                                                                                                             ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg        >  1:3952880/1‑150 (MQ=255)
                                                                                                                                              tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc       >  2:3906209/1‑150 (MQ=255)
                                                                                                                                                aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc     >  1:5284829/1‑150 (MQ=255)
                                                                                                                                                 aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGTGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt    >  2:6210599/1‑150 (MQ=255)
                                                                                                                                                 aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGTGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt    >  2:6211151/1‑150 (MQ=255)
                                                                                                                                                 aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt    <  1:4474556/150‑1 (MQ=255)
                                                                                                                                                   gAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGa  <  1:693501/150‑1 (MQ=255)
                                                                                                                                                   gAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGa  <  1:2499284/150‑1 (MQ=255)
                                                                                                                                                   gAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGa  <  2:3659460/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGA  >  NC_000913/2470284‑2470580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: