Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,725,844 G→T 6.6% intergenic (‑98/+225) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,725,8440GT 6.6% 1193.1 / 4.5 353intergenic (‑98/+225)kgtP/rrfGalpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base G (122/194);  new base T (35/2);  total (157/196)
Fisher's exact test for biased strand distribution p-value = 1.19e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 362 total aligned reads displayed.

GAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCG  >  NC_000913/2725695‑2725993
                                                                                                                                                      |                                                                                                                                                     
   tATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTTCATATCATTATTCaa                                                                                                                                                    >  2:784254/1‑150 (MQ=255)
       actactTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATAATATACATTTGTTTCATATAATTATTCAACCtt                                                                                                                                                >  2:6133143/1‑150 (MQ=255)
       actactTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTGTTGTTTATTTTTTTTGTAAAGGAAATATTATAAATTTGTTGCATATCATTTTTCAACCtt                                                                                                                                                >  1:3700946/1‑150 (MQ=255)
         tactTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTTTAAAGGAAAAAATAAACAAATTTTGCATAACATTATGCAACCATaa                                                                                                                                              >  1:765222/1‑150 (MQ=255)
            ttGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTGTTAAGGAAATATTATAAATTTGTTGCATATAATTATGCAACCTTAAccc                                                                                                                                           >  2:1881365/1‑149 (MQ=255)
            ttGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAAACTTAAACa                                                                                                                                           >  1:4251177/1‑150 (MQ=255)
                   tttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTTTAAAGGAAAAAATAAACAATTTTTTGATATAATTATTCAAACATAAACATTAAatt                                                                                                                                    >  1:2526726/1‑150 (MQ=255)
                   tttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCGTAACCATGAAttt                                                                                                                                    >  2:382679/1‑150 (MQ=255)
                    ttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATtta                                                                                                                                   <  2:3249458/150‑1 (MQ=255)
                    ttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATtta                                                                                                                                   <  1:258830/150‑1 (MQ=255)
                       cTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTTTTAATGAAATATTATACATTTTTTTCATATAATTATTCAACCATAAACAAGAAAttattt                                                                                                                                >  1:6524002/1‑150 (MQ=255)
                           cTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTTTTTAAGGTAATATTTTAAATTTTTTGGATATCATTATTAAAACATAAAAATTAAtttatttatc                                                                                                                             >  1:930641/1‑148 (MQ=255)
                            tGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAg                                                                                                                           <  1:2204302/150‑1 (MQ=255)
                             gCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTGTAAATGAAATATTATACATTTGTTGCATATCATTAATCAACCTTTAACATGAATTTAGTTAGCAgg                                                                                                                          >  2:6393171/1‑150 (MQ=255)
                                      ctACTTTCAGACATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGGGAGCAGGAAAATTGtt                                                                                                                 <  2:4247062/149‑1 (MQ=255)
                                       tACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTa                                                                                                                <  2:2444618/150‑1 (MQ=255)
                                         cTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTAtt                                                                                                              <  1:6405320/150‑1 (MQ=255)
                                          tttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTTTAAAGGAAATATAATAAAATAGTTTCAGATCATTATGCAACCATAAACAAGAATTTAGTTATCAGGAAAATgggtgttt                                                                                                             >  1:841406/1‑144 (MQ=255)
                                          tttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATAATTATGCAACCTTAAACATGAATTTAGTTAGCAGGAAAATGGTTATTg                                                                                                             >  2:5120077/1‑150 (MQ=255)
                                           ttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTga                                                                                                            <  2:6490461/150‑1 (MQ=255)
                                            tCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTgag                                                                                                           <  1:2763941/150‑1 (MQ=255)
                                            tCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTTTTTTTTTTTTTAATGGAAAATTATAAAATTTTTTGATATAAATATGGAACCATAAAAAATAAATTAGTTTGAAGGGAAATGGttgttgtg                                                                                                           >  1:2683813/1‑148 (MQ=255)
                                            tCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTgag                                                                                                           >  2:820102/1‑150 (MQ=255)
                                              aGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTgagga                                                                                                         <  2:3038174/150‑1 (MQ=255)
                                              aGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTTTTTTTTTTTTTAAGGGAATAATATAAAATTTTTTGATATAAATATTCAAACATAAAAAATAAATTAGTTATCATGAAAAtgtttgtttttgt                                                                                                         >  1:5771909/1‑145 (MQ=255)
                                               gccatgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTaa                                                                                                                                              <  1:6460088/112‑1 (MQ=255)
                                               gccatgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTgaggag                                                                                                        <  1:1903982/150‑1 (MQ=255)
                                               gccatgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTTTAAAGGAAATATTATAAAATTTTTTCATAAAATTATTCAACCATAAACAAGAAATTAGTTTTCAGGAAAAATGTTAttttgggg                                                                                                        >  1:4382154/1‑144 (MQ=255)
                                                  atgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAATTTGTTGCATATCATTATTCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGGTATTGAGGAGCtt                                                                                                     >  2:364191/1‑150 (MQ=255)
                                                    gccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTaa                                                                                                   <  1:4191361/150‑1 (MQ=255)
                                                    gccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTaa                                                                                                   <  2:1349793/150‑1 (MQ=255)
                                                     ccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAg                                                                                                  <  1:6218378/150‑1 (MQ=255)
                                                     ccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAg                                                                                                  <  1:4875244/150‑1 (MQ=255)
                                                       atTATGTCTCCTGCCGTCATCCGATGCTTTTGTCCGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGa                                                                                                <  1:3820830/150‑1 (MQ=255)
                                                        ttATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTTTAAAGGAAATATTATACATTTTTTTCATATCATTATTCAAACATAAACATGAATTTAGTTATCAGGAAAAAGGTTATTGGGGAGCTTAAGGAt                                                                                               >  2:2911968/1‑150 (MQ=255)
                                                          aTGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTTTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATaa                                                                                             >  2:227111/1‑150 (MQ=255)
                                                           tGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATaaa                                                                                            <  2:1298647/150‑1 (MQ=255)
                                                           tGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATaaa                                                                                            <  2:1126117/150‑1 (MQ=255)
                                                               tcCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAATGAAATATTATACATTTGTTGCATATCATTATGCAACATTAAACAAGAATTTATTTAGCAGGAAAATGGGTATTGAGGAGCTTAAGGATAAAttta                                                                                        >  2:4291429/1‑149 (MQ=255)
                                                                ccTGCCGTAATCCGATGCTTTTGTCGTTCGCTTTTTTTTATTTTTTTTGTAAAGGAAATATTATTCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAATTGGTTATTGAGGAGCTTAAGGATAAATTTCt                                                                                       <  1:1022400/150‑1 (MQ=255)
                                                                 cTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTg                                                                                      <  1:1924536/150‑1 (MQ=255)
                                                                  tGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATaaa                                                                                            <  1:2128639/143‑1 (MQ=255)
                                                                       tAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAAATTGTTGCATATAATTATTCAAACTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAagg                                                                                >  2:2010395/1‑150 (MQ=255)
                                                                         aTCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAaggag                                                                              <  2:3317942/150‑1 (MQ=255)
                                                                          tCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTGGAAAGGAAATATTATACAATTGTTGCATATCATTATGCAACCTTAAACAAGAATTTAGTTAGCAGGAAAATTGTTATTTAGGAGCTTAATGATAAAATTCTGGTAaggagg                                                                             >  2:4534291/1‑150 (MQ=255)
                                                                          tCCGATGCTGTTGTCGGTCGCGGTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCAATATGCAACCATAACCAACAATTTAGTTAGCACGAAAAAGGGTATTGAGGAGCATAACGAGAAAATTAGGGTAacgagg                                                                             >  2:3975070/1‑150 (MQ=255)
                                                                           ccGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAggagga                                                                            <  1:998609/150‑1 (MQ=255)
                                                                                  ttttGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGTAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTAt                                                                     <  2:5188382/150‑1 (MQ=255)
                                                                                  ttttGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTAt                                                                     <  1:4215726/150‑1 (MQ=255)
                                                                                  ttttGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTAt                                                                     <  1:4332442/150‑1 (MQ=255)
                                                                                   tttGTCGGTCGCTTTTGTTTATTTTTTTTTTAAAGGAAATATTATACAATTGTTTCATATAATTATGCAACCATAAACATGAAATTAGTTAGCAGGAAAAAGGTTATTTAGGAGCATAAGGATAAATTTATTGTAAGGATGACAAGTATg                                                                    >  2:2726597/1‑150 (MQ=255)
                                                                                        cggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGt                                                               <  1:2416053/150‑1 (MQ=255)
                                                                                        cggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGt                                                               <  2:5580756/150‑1 (MQ=255)
                                                                                        cggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGt                                                               <  1:2939158/150‑1 (MQ=255)
                                                                                         ggtcgCTTTTGTTTAGTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCCTGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTg                                                              <  1:139446/150‑1 (MQ=255)
                                                                                               ttttGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAg                                                        >  2:2991509/1‑150 (MQ=255)
                                                                                               ttttGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAg                                                        >  1:1606797/1‑150 (MQ=255)
                                                                                               ttttGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAg                                                        >  1:5287949/1‑150 (MQ=255)
                                                                                                  tGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTg                                                     <  1:1701984/150‑1 (MQ=255)
                                                                                                  tGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTg                                                     <  2:53511/150‑1 (MQ=255)
                                                                                                    tttATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTggg                                                   <  2:5725613/150‑1 (MQ=255)
                                                                                                     ttATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTTGTTAGCAGGAAAATGGTTATTGAGGTGGTTATGGATATATTTCTGGTAAGGAGGACACGTATGGAAGTGGGGAAGTTgggg                                                  >  2:2409201/1‑150 (MQ=255)
                                                                                                     ttATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTgggg                                                  >  1:5626311/1‑150 (MQ=255)
                                                                                                      tATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGa                                                 >  2:202960/1‑150 (MQ=255)
                                                                                                         ttttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAg                                               >  1:3128847/1‑150 (MQ=255)
                                                                                                         ttttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAg                                               <  2:2683764/150‑1 (MQ=255)
                                                                                                          tttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGc                                              <  2:2607321/150‑1 (MQ=255)
                                                                                                           ttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGTACACGTATGGAAGTGGGCAAGTTggtcccttc                                             >  1:5462041/1‑143 (MQ=255)
                                                                                                              tttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTa                                          <  1:6024138/150‑1 (MQ=255)
                                                                                                                tGTACAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATc                                        <  2:5395380/150‑1 (MQ=255)
                                                                                                                tGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATc                                        <  1:4742001/150‑1 (MQ=255)
                                                                                                                     aGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGATg                                   >  2:1488509/1‑150 (MQ=255)
                                                                                                                      ggAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGc                                  >  1:3045002/1‑150 (MQ=255)
                                                                                                                      ggAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGc                                  >  1:326972/1‑150 (MQ=255)
                                                                                                                       gAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCt                                 <  2:346998/150‑1 (MQ=255)
                                                                                                                          atatTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCACGTTGGGGAAGCCGTATCCGTTGCTGaa                              >  2:5260352/1‑150 (MQ=255)
                                                                                                                          atatTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGaa                              <  2:4809530/150‑1 (MQ=255)
                                                                                                                              tataCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGTTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGGATGCAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTg                          >  2:3111704/1‑150 (MQ=255)
                                                                                                                                taCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGc                        >  1:3535007/1‑150 (MQ=255)
                                                                                                                                        ttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGTGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTgg                <  1:5104664/150‑1 (MQ=255)
                                                                                                                                        ttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTgg                >  2:340512/1‑150 (MQ=255)
                                                                                                                                        ttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTgg                <  1:5615635/150‑1 (MQ=255)
                                                                                                                                        ttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTgg                <  1:5711245/150‑1 (MQ=255)
                                                                                                                                        ttgCATATCATTATGCAACCTTAACCATGAAGTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGACGTGGGCACGGTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATgggg                >  2:3179710/1‑150 (MQ=255)
                                                                                                                                          gCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGa              >  2:5863946/1‑150 (MQ=255)
                                                                                                                                          gCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGa              <  1:5965128/150‑1 (MQ=255)
                                                                                                                                          gCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGa              >  2:4996863/1‑150 (MQ=255)
                                                                                                                                           cATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAg             <  2:181758/150‑1 (MQ=255)
                                                                                                                                             tatCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGta           >  2:4420914/1‑150 (MQ=255)
                                                                                                                                             tatCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGta           <  1:5843350/150‑1 (MQ=255)
                                                                                                                                              atCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGtat          >  1:5396794/1‑150 (MQ=255)
                                                                                                                                               tCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGtata         >  2:1510964/1‑150 (MQ=255)
                                                                                                                                               tCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGtata         <  2:2888316/150‑1 (MQ=255)
                                                                                                                                               tCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGtata         <  2:5988836/150‑1 (MQ=255)
                                                                                                                                                cATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATaa        <  1:122103/150‑1 (MQ=255)
                                                                                                                                                cATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATaa        >  1:344372/1‑150 (MQ=255)
                                                                                                                                                 aTTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAg       <  2:2115373/150‑1 (MQ=255)
                                                                                                                                                 aTTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAg       <  2:859848/150‑1 (MQ=255)
                                                                                                                                                  ttATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGa      >  2:4134480/1‑150 (MQ=255)
                                                                                                                                                   tATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGAc     <  1:4027196/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCG  >  NC_000913/2725695‑2725993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: