BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,818,046	A→G	6.1%	intergenic (‑186/+13)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,818,046	0	A	G	6.1%	1025.4 / 34.7	323	intergenic (‑186/+13)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base A (169/126); new base G (6/13); total (175/148)
Fisher's exact test for biased strand distribution p-value = 3.33e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 329 total aligned reads displayed.

GCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAA  >  NC_000913/2817897‑2818193

gCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGCCTCGCCCTACGGGCCGTTTCTAAAGCAAAGATATCATCCCTAGTGCATGCGGATAATTCCGCTACCACGACGATAACATCATTGCTGAAGa                                                                                                                                                     >  2:1669619/1‑150 (MQ=17)

  tactaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAtt                                                                                                                                                   >  2:6356271/1‑150 (MQ=255)

  tactaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACATTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAtt                                                                                                                                                   >  1:863720/1‑150 (MQ=255)

    ctaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTc                                                                                                                                                 <  1:504057/150‑1 (MQ=255)

     taAAGCCATATCACTTCCGAGATGGTGCATCCGGGAGGAGTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCg                                                                                                                                                <  1:2039198/150‑1 (MQ=255)

     taAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCAGAGCGCCTCGCCCTGCGGGCCGATGCTAAAGCAACGATCTCATCCCTAGTGCCTGCGGTTAATTCCGCAACCTCTTCGATACCTTCATTGCTGAAGATTTCg                                                                                                                                                >  2:410906/1‑150 (MQ=17)

      aaaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCgg                                                                                                                                               <  2:6212527/150‑1 (MQ=255)

         gCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGgaga                                                                                                                                            >  1:5701250/1‑150 (MQ=255)

              ATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATg                                                                                                                                       <  1:5204087‑M1/150‑1 (MQ=255)

               tctaTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAACGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATgg                                                                                                                                      <  2:3987301/147‑1 (MQ=255)

               TCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATgg                                                                                                                                      >  2:2846406‑M1/1‑150 (MQ=255)

                   ttAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCa                                                                                                                                  >  1:6684030/1‑150 (MQ=255)

                   ttAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCa                                                                                                                                  >  1:3671067/1‑150 (MQ=255)

                        agaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCgg                                                                                                                             >  2:45837/1‑150 (MQ=255)

                         gaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCggg                                                                                                                            >  1:6486051/1‑150 (MQ=255)

                         gaTGGTGCATCCGGGAGGATTACTCGCCATGCGGATCGCCCGACGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGAGCCTGCGGTTAATTCCGCTACCTCAACGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCggg                                                                                                                            >  2:4375383/1‑150 (MQ=18)

                             gTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGggagga                                                                                                                        >  1:3334490/1‑150 (MQ=255)

                             gTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGggagga                                                                                                                        >  1:3632027/1‑150 (MQ=255)

                             gTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGggagga                                                                                                                        >  2:353832/1‑150 (MQ=255)

                               gCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTACGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGAtt                                                                                                                      <  2:245441/150‑1 (MQ=255)

                                  tCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga                                                                                                                                              <  1:1321341/123‑1 (MQ=255)

                                    cGGGAGGTTTACTCGCCGTGCGGCTCGCCCTTCGGGCCGTTGCTCTAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAAc                                                                                                                 <  1:5795736/150‑1 (MQ=255)

                                              aCTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGCATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGc                                                                                                       >  2:5545407/1‑150 (MQ=255)

                                               cTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCt                                                                                                      >  2:1301109/1‑150 (MQ=255)

                                               cTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCt                                                                                                      <  2:4205859/150‑1 (MQ=255)

                                                tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTc                                                                                                     >  2:905950/1‑150 (MQ=255)

                                                tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTc                                                                                                     >  2:1622438/1‑150 (MQ=255)

                                                tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTc                                                                                                     >  2:3110605/1‑150 (MQ=255)

                                                tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTc                                                                                                     >  1:3597818/1‑150 (MQ=255)

                                                  gCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAAATCCGCTACCTCTTCGATACCATCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAAACACCGACCGCTCgg                                                                                                   >  1:6390255/1‑150 (MQ=255)

                                                  gCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGGGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCCACCGCTCgg                                                                                                   >  1:4201762/1‑150 (MQ=255)

                                                   ccTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGt                                                                                                  <  1:1735256/150‑1 (MQ=255)

                                                     ttGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                                                                >  2:579107/1‑150 (MQ=255)

                                                           cTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGcc                                                                                          <  2:3154379/150‑1 (MQ=255)

                                                            tCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCg                                                                                         >  2:5323271/1‑150 (MQ=255)

                                                            tCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCg                                                                                         >  1:5582437/1‑150 (MQ=255)

                                                                ccTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTa                                                                                     >  2:6470156/1‑150 (MQ=255)

                                                                    cGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTActct                                                                                 >  1:5529422/1‑150 (MQ=255)

                                                                          gTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAg                                                                           >  1:3703190/1‑150 (MQ=255)

                                                                          gTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAg                                                                           <  1:2711573/150‑1 (MQ=255)

                                                                           ttGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGc                                                                          <  1:403740/150‑1 (MQ=255)

                                                                             gCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTg                                                                        >  2:4127086/1‑150 (MQ=255)

                                                                               tAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCCGCTGAg                                                                      >  1:3900522/1‑150 (MQ=255)

                                                                               tAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                                      >  2:2010075/1‑150 (MQ=255)

                                                                               tAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                                      >  1:1398352/1‑150 (MQ=255)

                                                                                aaaGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGc                                                                     >  1:4062502/1‑150 (MQ=255)

                                                                                   gCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAc                                                                  >  1:4556581/1‑150 (MQ=255)

                                                                                     aaCGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACgg                                                                <  2:5246505/150‑1 (MQ=255)

                                                                                      aCGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGa                                                               >  2:3196174/1‑150 (MQ=255)

                                                                                        gTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATg                                                             >  1:1009288/1‑150 (MQ=255)

                                                                                        gTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATACGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATg                                                             >  1:4317872/1‑150 (MQ=255)

                                                                                            tcatcCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATc                                                         >  1:4701515/1‑150 (MQ=255)

                                                                                             catcCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCg                                                        <  1:6356271/150‑1 (MQ=255)

                                                                                             catcCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCg                                                        >  1:947832/1‑150 (MQ=255)

                                                                                                   tAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAat                                                  >  2:4737051/1‑150 (MQ=255)

                                                                                                   tAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAat                                                  >  2:1378283/1‑150 (MQ=255)

                                                                                                        ccTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatttatt                                             >  1:4910439/1‑150 (MQ=255)

                                                                                                           gCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTa                                          <  2:3671067/150‑1 (MQ=255)

                                                                                                           gCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTa                                          <  1:804028/150‑1 (MQ=255)

                                                                                                           gCGGTTAAGTCCTCGACCTCTTCGATACGCTCATAGCGGAAGATCTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTa                                          <  1:1649021/150‑1 (MQ=14)

                                                                                                               ttAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCGACGGATGCATCGGGAAATTTATTTTActgc                                      >  1:3033409/1‑150 (MQ=255)

                                                                                                                 aagtCCGCTACCTCTTCGATACCTTCAGTGCTGAAGATTTCGGAGAATCTGGCGCATGCGGTAGGTTTCGAACCTCCGACCTCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgctg                                    <  1:6036523/147‑1 (MQ=14)

                                                                                                                  aTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa                                   >  2:5127822/1‑150 (MQ=255)

                                                                                                                  aTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa                                   <  2:2352044/150‑1 (MQ=255)

                                                                                                                  aTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa                                   <  1:3778035/150‑1 (MQ=255)

                                                                                                                  aTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa                                   <  1:4769982/150‑1 (MQ=255)

                                                                                                                    tCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGata                                 <  2:3359289/150‑1 (MQ=255)

                                                                                                                      cGCTATCTCTTCGATACCTTCATTGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATAtt                               <  2:4480644/150‑1 (MQ=16)

                                                                                                                      cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATAtt                               <  2:1693341/150‑1 (MQ=255)

                                                                                                                      cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATAtt                               <  1:744903/150‑1 (MQ=255)

                                                                                                                      cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATAtt                               <  1:4127086/150‑1 (MQ=255)

                                                                                                                       tcTACCTCTTCTATACCTTCATTGCTGAAGATTTAGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGAttttt                              <  2:1398352/149‑2 (MQ=14)

                                                                                                                       gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTg                              <  1:6523643/150‑1 (MQ=255)

                                                                                                                        cTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGa                             <  1:1139234/150‑1 (MQ=255)

                                                                                                                        cTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGAtttttt                             <  2:3703190/150‑3 (MQ=255)

                                                                                                                         tACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTACTGCTGATATTGAt                            >  2:2707339/1‑150 (MQ=255)

                                                                                                                             tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATg                                                                                                                                       <  1:5189187/39‑1 (MQ=255)

                                                                                                                             tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATAcag                        >  2:4808156/1‑148 (MQ=255)

                                                                                                                             tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCg                        >  1:2322306/1‑150 (MQ=255)

                                                                                                                             tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCAGATATTTATAACg                        >  2:6495533/1‑150 (MQ=255)

                                                                                                                             tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTACTGCTGATATTGATAcag                        >  2:3590018/1‑148 (MQ=255)

                                                                                                                             tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAGCGGGAAACTTAGTTTACTGCAGAttttttgatac                        >  1:4371340/1‑143 (MQ=11)

                                                                                                                             tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCAGAGAACTCTATCCAGCTGAGCTACGGATGCATCGGGAACATTATTTTACTCCTGATATTGATACCg                        >  2:6088934/1‑150 (MQ=11)

                                                                                                                              ctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGGTGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGc                       <  1:6549889/150‑1 (MQ=255)

                                                                                                                               ttCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGct                      >  2:5991652/1‑150 (MQ=18)

                                                                                                                               ttCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATGTATTTTAATGCTGATAATGATAACGca                      >  2:3804086/1‑149 (MQ=11)

                                                                                                                               ttCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGct                      <  1:1245343/150‑1 (MQ=255)

                                                                                                                                tCGATACCTTCATTGCTGAAGGTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGcta                     <  1:1179529/150‑1 (MQ=38)

                                                                                                                                 cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGctac                    <  1:6116765/150‑1 (MQ=255)

                                                                                                                                  gATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGctact                   >  1:2420947/1‑150 (MQ=255)

                                                                                                                                     aCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTaaa                <  1:5127822/150‑1 (MQ=255)

                                                                                                                                      ccTTCATTGATGAAGATTTCGGTGATTATGGTGGTTCCGTGTGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCTAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAg               <  1:1476451/150‑1 (MQ=14)

                                                                                                                                       cTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAGTTTACTGCTGATATTGATAACGctagaatagc              >  2:1232985/1‑143 (MQ=255)

                                                                                                                                        ttCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCAGGAAATTTATTTTACTGCTGATATTGATACCGCTACTTAAGcc             <  1:3238652/150‑1 (MQ=255)

                                                                                                                                        ttCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGACATTTATTTTACTGCTGATATTGATACCGCTACTAAAGcc             >  2:686412/1‑150 (MQ=255)

                                                                                                                                        ttCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGcc             <  1:1622438/150‑1 (MQ=255)

                                                                                                                                          cATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCat           <  2:4549413/150‑1 (MQ=255)

                                                                                                                                                tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTTATAACGCTCCTAAAGCCATATCAAg     >  1:5916298/1‑150 (MQ=255)

                                                                                                                                                tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACATCCCACCGCTCGGTACGTAGCCGAGAACTCTATCCACCTGAGCTACTGATCCCTCGGGAAATTAAATTTACTGCTGAGATTGATACCGCTACTCTAGCCATATCCAg     >  2:1550780/1‑150 (MQ=14)

                                                                                                                                                   aGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCGCCTAAAGCCATATCAAGTaa  >  1:2453725/1‑150 (MQ=255)

GCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAA  >  NC_000913/2817897‑2818193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`