BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,818,049	T→A	6.0%	intergenic (‑189/+10)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,818,049	0	T	A	6.0%	1097.2 / 38.4	322	intergenic (‑189/+10)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base T (168/134); new base A (6/14); total (174/148)
Fisher's exact test for biased strand distribution p-value = 3.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 323 total aligned reads displayed.

CTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAA  >  NC_000913/2817901‑2818193

 taAAGCCATATCACTTCCGAGATGGTGCATCCGGGAGGAGTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCg                                                                                                                                                <  1:2039198/150‑1 (MQ=255)

 taAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCAGAGCGCCTCGCCCTGCGGGCCGATGCTAAAGCAACGATCT‑CATCCCTAGTGCCTGCGGTTAATTCCGCAACCTCTTCGATACCTTCATTGCTGAAGATTTCg                                                                                                                                                >  2:410906/1‑150 (MQ=17)

  aaaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCgg                                                                                                                                               >  1:4775531/1‑150 (MQ=255)

   aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga                                                                                                                                              >  1:3815830/1‑150 (MQ=255)

   aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga                                                                                                                                              <  1:1304487/150‑1 (MQ=255)

   aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga                                                                                                                                              <  2:4250510/150‑1 (MQ=255)

         TATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAatat                                                                                                                                        <  1:2436737‑M1/150‑1 (MQ=255)

         TATCAATTAAGAGATGGTGCATCCGGCAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAatat                                                                                                                                        <  2:5493073‑M1/150‑1 (MQ=255)

          ATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCATTGCGGCTCGCCATACGGGCCGTTGCTAAAGCAACGTTAT‑AATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCAGTGCTGATGATTTCGGAGAATATg                                                                                                                                       <  1:6087328‑M1/150‑1 (MQ=255)

           tctaTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAACGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATgg                                                                                                                                      <  2:3987301/147‑1 (MQ=255)

           TCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATgg                                                                                                                                      >  1:5277769‑M1/1‑150 (MQ=255)

            CAATTAAGAGATGGTGCCTCCGGGAGGATTACTCGCCTGGCGGCTCGCCCTTCGGGCCGTTGCTAAATCAACGTTAT‑CCTCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGt                                                                                                                                     >  2:1586292‑M1/1‑150 (MQ=255)

            CAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGt                                                                                                                                     >  1:5191870‑M1/1‑150 (MQ=255)

               ttAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCa                                                                                                                                  >  1:6684235/1‑150 (MQ=255)

                  agagATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTTAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCTTcc                                                                                                                               <  1:5072608/150‑1 (MQ=255)

                    agaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCgg                                                                                                                             >  2:45901/1‑150 (MQ=255)

                    agaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCgg                                                                                                                             <  2:3117412/150‑1 (MQ=255)

                     gaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCggg                                                                                                                            >  1:3186024/1‑150 (MQ=255)

                         gTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGggagga                                                                                                                        >  1:4377026/1‑150 (MQ=255)

                           gccgccGGGCGGACGACCCGCCTCGCGGCGCGGCCTCAGCGCCGTTGCCAAAGCGACGCTATGCCGACCT‑GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGAtt                                                                                                                      <  2:2115436/146‑1 (MQ=9)

                           gCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGAtt                                                                                                                      <  2:2934994/150‑1 (MQ=255)

                             aTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCg                                                                                                                    >  1:5821628/1‑150 (MQ=255)

                                cGGGAGCATTACTCGCCCTGCGGCTCGCCCTTCGGGCCGTTGCTACAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAAc                                                                                                                 <  1:760753/150‑1 (MQ=255)

                                        ttACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGAcc                                                                                                         <  1:897838/150‑1 (MQ=255)

                                          aCTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGc                                                                                                       >  2:138366/1‑150 (MQ=255)

                                          aCTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGc                                                                                                       >  1:4021658/1‑150 (MQ=255)

                                           cTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCt                                                                                                      >  1:6539864/1‑150 (MQ=255)

                                           cTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCt                                                                                                      >  1:356871/1‑150 (MQ=255)

                                            tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTc                                                                                                     >  2:905950/1‑150 (MQ=255)

                                              gCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTCGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCgg                                                                                                   >  2:1425500/1‑150 (MQ=255)

                                              gCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCgg                                                                                                   >  2:1139234/1‑150 (MQ=255)

                                               ccTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGt                                                                                                  <  1:1735256/150‑1 (MQ=255)

                                                 atGCCGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTAACTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                                                                <  1:5175148/149‑1 (MQ=255)

                                                      gCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGc                                                                                           >  2:6321723/1‑150 (MQ=255)

                                                       cTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGAtt                                                                                                                      >  2:1633449/1‑122 (MQ=255)

                                                       cTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGAtt                                                                                                                      <  1:1633449/122‑1 (MQ=255)

                                                       cTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGcc                                                                                          <  2:3154379/150‑1 (MQ=255)

                                                        tCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGGGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGGTCGTAGCCg                                                                                         >  1:1512494/1‑150 (MQ=255)

                                                        tCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCg                                                                                         >  2:5323271/1‑150 (MQ=255)

                                                        tCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGGTCGTAGCCg                                                                                         >  1:5935386/1‑150 (MQ=255)

                                                           cccTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCCTCCGGGAGGATGCGAACCTCCGACCGCTCGGTTCGTGGCCGAGt                                                                                      >  2:3551269/1‑150 (MQ=255)

                                                            ccTTCGGGCCGTTGCTGACGCAACTATAA‑CCATCCTCGTGCCTGCGGTTAATTCCGCTATCGCTTCTTTACCTTCATTGCTCAAGGTAAAGGAGAAACTGGTGCATCCGGGAGGATGCGAACCTCCGTCCGCTCGGGTCGTTGCCGAGTa                                                                                     >  2:2786817/1‑150 (MQ=255)

                                                                cGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTActct                                                                                 >  1:5529422/1‑150 (MQ=255)

                                                                  ggCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTAt                                                                               >  2:6474111/1‑150 (MQ=255)

                                                                      gTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAg                                                                           <  1:2710544/150‑1 (MQ=255)

                                                                        tGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                                                         <  1:4755635/150‑1 (MQ=255)

                                                                         gCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTg                                                                        >  1:6556534/1‑150 (MQ=255)

                                                                           tAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                                      >  1:1398352/1‑150 (MQ=255)

                                                                                cAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACg                                                                 >  2:413890/1‑150 (MQ=255)

                                                                                 aaCGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACgg                                                                <  2:5246505/150‑1 (MQ=255)

                                                                                  aCGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGa                                                               >  2:3196279/1‑150 (MQ=255)

                                                                                    gTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATg                                                             >  1:1009288/1‑150 (MQ=255)

                                                                                    gTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACATAAATTGCTTAAGATTTCGTAGAATATGGTGCATCAGGGTGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCGACGGATg                                                             >  1:4317888/1‑150 (MQ=14)

                                                                                     ttAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGc                                                            <  2:4551814/150‑1 (MQ=255)

                                                                                     ttAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGc                                                            >  1:4200844/1‑150 (MQ=255)

                                                                                          catcCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCg                                                        <  1:45901/150‑1 (MQ=255)

                                                                                               cTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaaa                                                   <  2:2354512/150‑1 (MQ=255)

                                                                                                   tGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAattta                                               >  2:1337637/1‑150 (MQ=255)

                                                                                                      cTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATACAGCTGAGCTACGGATGCATCGGGAAatttattt                                            >  1:6253029/1‑150 (MQ=255)

                                                                                                        gCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTa                                          <  2:3671393/150‑1 (MQ=255)

                                                                                                            ttAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgc                                      >  2:6195610/1‑150 (MQ=255)

                                                                                                               aTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa                                   <  1:3777687/150‑1 (MQ=255)

                                                                                                                ttCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGat                                  >  1:5633740/1‑150 (MQ=255)

                                                                                                                ttCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTACTGCTGat                                  >  2:1264495/1‑150 (MQ=255)

                                                                                                                ttCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTACTGCTGat                                  >  2:4810180/1‑150 (MQ=255)

                                                                                                                   cGCTATCTCTTCGATACCTTCATTGCTGAAGGTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATAtt                               <  2:4481205/150‑1 (MQ=25)

                                                                                                                    gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATACAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGATGATATTg                              >  1:3028108/1‑150 (MQ=255)

                                                                                                                    gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCAGGCAGCTCCGAACGTAGCAGAGAACTCAAACCAGATGAGCGACGGATGCAGCGGGATCGTTATTTTACTGCTGagtgtg                              >  1:6447303/1‑145 (MQ=14)

                                                                                                                     cTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGa                             <  1:1139234/150‑1 (MQ=255)

                                                                                                                      tACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGAt                            >  2:4399499/1‑150 (MQ=255)

                                                                                                                        ccTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAGCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATAc                          <  1:6372333/150‑1 (MQ=255)

                                                                                                                          tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTTATAcag                        >  1:2322104/1‑148 (MQ=255)

                                                                                                                          tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCAGATATTTATAACg                        >  2:6495533/1‑150 (MQ=255)

                                                                                                                          tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTAGTTTACTGCTGATATTGATAcag                        >  2:3590018/1‑148 (MQ=255)

                                                                                                                          tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCAGAGAACTCTATCCAGCTGAGCTACGGATGCATCGGGAACATTATTTTACTCCTGATATTGATACCg                        >  2:6088934/1‑150 (MQ=11)

                                                                                                                            ttCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGAt                            >  2:371116/1‑144 (MQ=18)

                                                                                                                            ttCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGct                      >  2:5991652/1‑150 (MQ=18)

                                                                                                                            ttCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATGTATTTTAATGCTGATAATGATAACGca                      >  2:3804086/1‑149 (MQ=11)

                                                                                                                            ttCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGct                      <  1:1245343/150‑1 (MQ=255)

                                                                                                                            ttCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGGTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGACAGTTCGTTTACTGCTGATATTGATCCCGcg                      >  2:3420136/1‑149 (MQ=14)

                                                                                                                             tCGATACCTTCATTGCTGAAGGTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGcta                     <  1:1179529/150‑1 (MQ=38)

                                                                                                                             tCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGcta                     >  1:3668108/1‑150 (MQ=255)

                                                                                                                               gATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGctact                   <  1:3688629/150‑1 (MQ=255)

                                                                                                                                   ccTTCATTGATGAAGATTTCGGTGATTATGGTGGTTCCGTGTGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCTAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAg               <  1:1476451/150‑1 (MQ=14)

                                                                                                                                    cTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGc              <  1:2363106/150‑1 (MQ=255)

                                                                                                                                    cTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATAACGctactgcaca              >  1:814571/1‑145 (MQ=255)

                                                                                                                                     ttCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGACATTTATTTTACTGCTGATATTGATACCGCTACTAAAGcc             >  2:686412/1‑150 (MQ=255)

                                                                                                                                       cATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTATAGCCat           <  1:36230/150‑1 (MQ=16)

                                                                                                                                       cATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCat           <  2:2679980/150‑1 (MQ=255)

                                                                                                                                        aTTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATAAAGCTACTAAAGACaaa          >  1:4124631/1‑148 (MQ=255)

                                                                                                                                            cTGAAGATTTCGGAGAATATTGTGCATCCGTGAGGATTCGAACCTACGACCGATCGGTTCGTTGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCaa      <  1:4049864/150‑1 (MQ=14)

                                                                                                                                             tGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAg     >  1:6516498/1‑150 (MQ=21)

                                                                                                                                             tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaaact                                                 <  2:4765918/106‑3 (MQ=25)

                                                                                                                                             tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCatat         <  1:4931556/146‑1 (MQ=255)

                                                                                                                                             tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAGTTTACTGCAGATATTGATACCGCTACTAAAGCCATATCAAg     >  2:5149430/1‑150 (MQ=255)

                                                                                                                                             tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGACTCCACTAACCACCTCAGCACCCCATGCAACGGGAAAATTATAGTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAg     >  1:3328578/1‑150 (MQ=11)

                                                                                                                                              gAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTa                                                                                             <  2:5710493/61‑1 (MQ=40)

                                                                                                                                              gAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTa                                                                                             >  1:5710493/1‑61 (MQ=40)

                                                                                                                                              gAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGt    >  2:1981454/1‑150 (MQ=255)

                                                                                                                                                  aTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACgg                                                                >  1:4252940/1‑86 (MQ=35)

CTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTAT‑CATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAA  >  NC_000913/2817901‑2818193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`