Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,269,956 T→C 5.8% intergenic (+354/+260) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,269,9560TC 5.8% 1008.5 / 31.8 350intergenic (+354/+260)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base T (99/210);  new base C (9/10);  total (128/222)
Fisher's exact test for biased strand distribution p-value = 2.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 353 total aligned reads displayed.

CGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGG  >  NC_000913/3269807‑3270105
                                                                                                                                                     |                                                                                                                                                     
cgccgcATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGt                                                                                                                                                       <  2:3033339/150‑1 (MQ=255)
   cgcATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTaaa                                                                                                                                                    <  2:5322629/150‑1 (MQ=255)
     cATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTaaaaa                                                                                                                                                  <  1:4902359/150‑1 (MQ=255)
     cATCCGGCATGAATAAAGCGCACTAAACAATCTGAAACCCTCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATTAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTaaaaa                                                                                                                                                  <  1:5054380/150‑1 (MQ=255)
      aTCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAAc                                                                                                                                                 >  2:5767054/1‑150 (MQ=255)
      aTCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAAc                                                                                                                                                 >  1:3822694/1‑150 (MQ=255)
      aTCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAc                                                                                                                                                 >  2:3483137/1‑150 (MQ=255)
       tCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAAcc                                                                                                                                                >  1:4217142/1‑150 (MQ=255)
          ggCATGAATACAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCGTTCATCTTTCTGCCCCTCCAAAAGTAAAAACccgc                                                                                                                                             <  2:4679162/150‑1 (MQ=255)
            cATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGGAAAAACccgccg                                                                                                                                           >  1:2023085/1‑150 (MQ=255)
              tGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGaa                                                                                                                                         >  2:4269132/1‑150 (MQ=255)
               taaTAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACTTACATCAGTTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAATTAAAAACCCGCCGAAg                                                                                                                                        <  1:1493437/149‑1 (MQ=255)
                aaTAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGc                                                                                                                                       >  2:5658101/1‑150 (MQ=255)
                  tAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGCCAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCgg                                                                                                                                     >  1:4629705/1‑150 (MQ=255)
                  tAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGccg                                                                                                                                     <  1:5472781/150‑3 (MQ=255)
                  tAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCgg                                                                                                                                     <  1:3988851/150‑1 (MQ=255)
                  tAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                                                                     <  2:4482797/150‑1 (MQ=255)
                  tAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTAATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCgg                                                                                                                                     <  1:4525678/150‑1 (MQ=255)
                   aaaGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCggg                                                                                                                                    >  2:1331198/1‑150 (MQ=255)
                     aGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGtt                                                                                                                                  <  1:1444198/150‑1 (MQ=255)
                      gcgcACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCTTCGGCGGGttt                                                                                                                                 <  2:6562021/150‑1 (MQ=255)
                      gcgcACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCTTCGGCGGGttt                                                                                                                                 <  1:1705468/150‑1 (MQ=255)
                      gcgcACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGttt                                                                                                                                 >  1:747055/1‑150 (MQ=255)
                         cACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                                                              >  2:4793953/1‑150 (MQ=255)
                          aCTCAACAATCTGAAACCCGCCGGAACGGTTTATTCCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAAGAAAAAACACGCCGAAGCGGGttttaga                                                                                                                             >  2:292805/1‑147 (MQ=17)
                          aCTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                                                             <  2:6113905/150‑1 (MQ=255)
                           cTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACg                                                                                                                            <  1:2154453/150‑1 (MQ=255)
                           cTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGTAAAAAACCGCCGAAGCGGGTTTTTACg                                                                                                                            >  2:3665677/1‑150 (MQ=255)
                            tCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGt                                                                                                                           >  1:368532/1‑150 (MQ=255)
                            tCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGt                                                                                                                           >  1:2370783/1‑150 (MQ=255)
                             caacaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTa                                                                                                                          >  1:951103/1‑150 (MQ=255)
                              aacaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGtca                                                                                                                         >  1:3601831/1‑148 (MQ=255)
                              aacaaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaa                                                                                                                         >  1:2245448/1‑150 (MQ=255)
                                caaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaaaa                                                                                                                       <  2:3734266/150‑1 (MQ=255)
                                caaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCATTCTGCCCCTCCAACAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaaaa                                                                                                                       <  1:3300981/150‑1 (MQ=255)
                                  aTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCa                                                                                                                     <  2:2895229/150‑1 (MQ=255)
                                   tCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAg                                                                                                                    <  1:5279034/150‑1 (MQ=255)
                                   tCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTACAAAAGTAAAAACCCGCCGAAGCGGGTTTTTAAGTAAAACAg                                                                                                                    >  1:3237774/1‑150 (MQ=255)
                                    cTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg                                                                                                                   <  2:2967864/150‑1 (MQ=255)
                                         aCCCGCCGGACCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAACAGTAACAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaaa                                                                                                              <  2:6401789/150‑1 (MQ=255)
                                         aCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaaa                                                                                                              <  1:2753123/150‑1 (MQ=255)
                                             gccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGa                                                                                                          <  1:5887720/150‑1 (MQ=255)
                                              ccgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCCAAAGTTAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAc                                                                                                         <  2:6201996/150‑1 (MQ=255)
                                               cgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAcc                                                                                                        <  2:5035929/150‑1 (MQ=255)
                                                 gAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCATTCTGACACAACAAAAGTAAAAACCCGACGAAGCGGGTGTTTACGTAATACAGGTGAAACTGACAGa                                                                                                      >  1:2316171/1‑150 (MQ=17)
                                                      gTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGc                                                                                                 >  1:3054475/1‑150 (MQ=255)
                                                       tttATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTTAGTAAAAAAGGTGAAACTGACCGATAAGcc                                                                                                >  1:1860896/1‑150 (MQ=255)
                                                       tttATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGcc                                                                                                <  2:1456476/150‑1 (MQ=255)
                                                         tattaCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCgg                                                                                              <  2:2156525/150‑2 (MQ=255)
                                                            taCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGcttt                                                                                           <  1:454908/150‑1 (MQ=255)
                                                                 aCATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                      <  1:4771525/150‑1 (MQ=40)
                                                                  cATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                     <  2:6374838/150‑1 (MQ=39)
                                                                   ctCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGTAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                    <  1:2877531/149‑1 (MQ=38)
                                                                   agcAGGTAACACTGACCGATAAGCCGCTTTCTGTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                    <  1:3440457/148‑1 (MQ=255)
                                                                   aTCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                    <  1:2739192/150‑1 (MQ=38)
                                                                   aTCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                    <  2:5579644/150‑1 (MQ=38)
                                                                     cAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGt                                                                                  <  2:6365902/150‑1 (MQ=37)
                                                                       ggTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtat                                                                                <  1:5767054/150‑1 (MQ=37)
                                                                            aaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAgtgt                                                                           >  1:4136662/1‑150 (MQ=37)
                                                                             aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGTAAACACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTc                                                                          <  1:1005940/150‑1 (MQ=255)
                                                                             aCTGACCGAGCAGCCGCTTTCTTTGGGGTAGCGTGTCGTGGACAGTCCTTCCTCTGTCGGCCCCTCCACCAGTACAAACCCGCCGCCGCGGGTTTTTACGTAACACAGGTGAAACTGACCGATACGCCGCTTTCTTTTGGGTATAGTGTc                                                                          <  2:4889974/150‑1 (MQ=11)
                                                                               tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGGCAGTCAGTCATCATTCTGCCCCACCAAAAGTAAAAAACCCCCGAAGCGGGGTTTTACGTAAAACAGGTGAAAATGACCGATAAGCCGCTTTCTTTTGGGGGTAGTGGCgg                                                                        >  1:2609735/1‑149 (MQ=11)
                                                                                  ccGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACCGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGa                                                                     <  2:4627104/150‑1 (MQ=37)
                                                                                         gCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcat                                                              <  2:2270378/150‑1 (MQ=37)
                                                                                              tttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATc                                                         <  2:5849583/150‑1 (MQ=37)
                                                                                                  ttttGGGTATAGTGTCGTGGACAGTCATTAATCTTTCTGCCCCACCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTAAGTAAAAAAGGTGAAACTGAACCATAAGCCGCGTTCTGTTGGGGATATTGTCGGGGAAAGtaaatcaacattg                                                     >  1:6214234/1‑144 (MQ=11)
                                                                                                       ggTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGccc                                                <  2:5510385/150‑1 (MQ=37)
                                                                                                        gTATAGTGTCGTTGAATGTCATTCATCTTTCTGCCCCTCCAAAAGTATAAACCAGCCGAATCGGGTTTTTACGTAAAACAGTTTAATCTGACCGTTATGCCGCTTTCTTTTGGGTTTATTGTCGTGGACAGTCATTCATCTTTCTTcccc                                               <  1:1102377/150‑1 (MQ=11)
                                                                                                          ataGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTc                                             <  1:4419845/150‑1 (MQ=37)
                                                                                                             gtgtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaa                                          <  1:343202/150‑1 (MQ=37)
                                                                                                               gtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                        <  1:3308029/150‑1 (MQ=37)
                                                                                                               gtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                        <  2:2370783/150‑1 (MQ=37)
                                                                                                                tCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAg                                       >  2:1063431/1‑150 (MQ=37)
                                                                                                                    ggACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaa                                   <  2:4328013/150‑1 (MQ=37)
                                                                                                                      aCAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCTCTCCAAAAGCaaaaa                                 <  1:878621/150‑1 (MQ=37)
                                                                                                                           cattcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACTTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATTGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCAAAAAGCAAAAACccgc                            <  1:609471/150‑1 (MQ=255)
                                                                                                                            attcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                           <  2:327900/150‑1 (MQ=37)
                                                                                                                               catCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGaa                        <  1:4822250/150‑1 (MQ=37)
                                                                                                                                 tCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAAc                                                                                                                      <  1:5415802/54‑1 (MQ=40)
                                                                                                                                 tCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAc                                                                    >  2:2079502/1‑104 (MQ=17)
                                                                                                                                  cTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttcttt                                                                                       >  2:4564917/1‑84 (MQ=255)
                                                                                                                                   tttCTGCCCCTCCAACAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                    <  1:2512783/150‑1 (MQ=255)
                                                                                                                                   tttCTGCCCCTCCAAAAGTAAAAACCCGCCGTAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                    <  2:689609/150‑1 (MQ=37)
                                                                                                                                   tttCGGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                    <  2:5956209/150‑1 (MQ=37)
                                                                                                                                    gtCTGCCCCTCCAAAAGTAACAACCCGCCGAAGCGGGTTTTTACGTAAAACCGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCggg                   <  2:1783378/149‑1 (MQ=255)
                                                                                                                                      cTGCCCCTCCACAAGTAAAAACCCGCCGCAGCGGGTTTTTACGTAACACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAGTCATCTTACTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtt                 <  2:2614170/150‑1 (MQ=11)
                                                                                                                                      cTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtt                 >  1:3520709/1‑150 (MQ=37)
                                                                                                                                        gCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt               <  2:3869094/150‑1 (MQ=37)
                                                                                                                                         ccccTCCAAAAGTAAAACCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt              <  2:1860896/150‑1 (MQ=37)
                                                                                                                                         ccccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGttttt              >  1:3741513/1‑150 (MQ=37)
                                                                                                                                         ccccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt              >  1:6435832/1‑150 (MQ=37)
                                                                                                                                          cccTCCACCAGTATAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa             <  2:3431312/150‑1 (MQ=255)
                                                                                                                                          cccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGGTTTTTa             >  2:1052311/1‑150 (MQ=37)
                                                                                                                                          cccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa             <  1:1665609/150‑1 (MQ=37)
                                                                                                                                           ccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTGTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCAACTTTCTGCCCCTCCAATAGCAAAAACCCGCCGAAGCGGGTTTTTAc            >  2:5917760/1‑150 (MQ=255)
                                                                                                                                            cTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGCAAGTCATTCATCGTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTGTGTACg           >  2:4935080/1‑150 (MQ=11)
                                                                                                                                              ccAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTa         >  1:6603044/1‑150 (MQ=21)
                                                                                                                                                  aaGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCACAAGCAAAAACCCGCCGACGCGGGTTTTTACGTAAAcc     >  2:4112643/1‑150 (MQ=255)
                                                                                                                                                   aGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCa    <  2:3905488/150‑1 (MQ=39)
                                                                                                                                                    gTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg   <  2:2789509/150‑1 (MQ=38)
                                                                                                                                                     |                                                                                                                                                     
CGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGG  >  NC_000913/3269807‑3270105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: