Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,046,787 A→C 6.6% intergenic (‑185/‑179) yihG ← / → polA inner membrane protein, inner membrane acyltransferase/5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,046,7870AC 6.6% 532.8 / 21.1 150intergenic (‑185/‑179)yihG/polAinner membrane protein, inner membrane acyltransferase/5' to 3' DNA polymerase and 3' to 5'/5' to 3' exonuclease
Reads supporting (aligned to +/- strand):  ref base A (69/71);  new base C (2/8);  total (71/79)
Fisher's exact test for biased strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

TAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTAC  >  NC_000913/4046638‑4046936
                                                                                                                                                     |                                                                                                                                                     
tAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTaaaa                                                                                                                                                       <  2:100018/150‑1 (MQ=255)
 aGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTaaaaa                                                                                                                                                      <  1:1096642/150‑1 (MQ=255)
 aGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTaaaaa                                                                                                                                                      <  1:512141/150‑1 (MQ=255)
  gCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAg                                                                                                                                                     <  1:2303638/150‑1 (MQ=255)
    aGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCa                                                                                                                                                   <  2:1082229/150‑1 (MQ=255)
     gAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCAt                                                                                                                                                  <  2:2313884/150‑1 (MQ=255)
          cGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTTTTTACAGCATGatgt                                                                                                                                             <  2:2213011/150‑3 (MQ=255)
           gACCCAAATACCGGATGATCCTTAAGGAGAAAAATAATTCATATCTATACACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTTTTTTTTACTGCATGAAATACAATGACAAACATCATGCTTTTTACAGCATGatgtt                                                                                                                                            <  2:2418185/150‑4 (MQ=255)
            aCCCAACTAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTTTTTACAGCATGatgttt                                                                                                                                           <  1:166722/150‑5 (MQ=255)
            aCCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTTTTTACAGCATGatgttt                                                                                                                                           <  1:166807/150‑5 (MQ=255)
             cccAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCATTTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCCGTAAAAAGCATGAtaataa                                                                                                                                          <  2:2030842/150‑1 (MQ=255)
              ccAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATaaa                                                                                                                                         >  2:1879915/1‑150 (MQ=255)
              ccAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATaaa                                                                                                                                         <  1:1306768/150‑1 (MQ=255)
              ccAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATaaa                                                                                                                                         <  1:1306830/150‑1 (MQ=255)
              ccAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATaaa                                                                                                                                         >  1:807672/1‑150 (MQ=255)
              ccAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATaaa                                                                                                                                         >  1:497303/1‑150 (MQ=255)
                aaaTAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAAtt                                                                                                                                       >  1:101852/1‑150 (MQ=255)
                aaaTAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAAtt                                                                                                                                       >  1:2337506/1‑150 (MQ=255)
                        gatgatCCTTAATGATAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGa                                                                                                                               <  2:1502686/150‑1 (MQ=255)
                        gatgatCCTTAATGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGa                                                                                                                               <  2:2654628/150‑1 (MQ=255)
                        gatgatCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGTGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGa                                                                                                                               <  2:1903996/150‑1 (MQ=255)
                        gatgatCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGa                                                                                                                               >  2:2271369/1‑150 (MQ=255)
                        gatgatCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATAAAAAGCGa                                                                                                                               >  1:1657031/1‑150 (MQ=255)
                         atgatCCTTAAGTAGAAAAATAATTCATATCTATCCAAATTAGAAAAAATCCCATTATCTCAATTATTTGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGAt                                                                                                                              <  1:2736496/150‑1 (MQ=255)
                         atgatCCTTAAGGAGAAAAATAATTCTTATCTATCCACATTATAAAAAATCCCATTATCTCAATTATTAGGGTTGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGAt                                                                                                                              <  1:2736512/150‑1 (MQ=255)
                         atgatCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGAt                                                                                                                              <  2:902079/150‑1 (MQ=255)
                             tCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTaa                                                                                                                          >  2:1769542/1‑150 (MQ=255)
                                ttAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAaata                                                                                                                       >  2:2721846/1‑150 (MQ=255)
                                  aaGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAaataat                                                                                                                     <  1:403399/150‑1 (MQ=255)
                                     tagaAAAATAATTCATATCTATCCACATTAGAAAAAATCCAATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTa                                                                                                                  <  1:2305374/149‑1 (MQ=255)
                                     gagaAAAATAATTCATATCTATCCACATTATAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTa                                                                                                                  <  1:1925049/150‑1 (MQ=255)
                                     gagaAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTa                                                                                                                  >  2:305091/1‑150 (MQ=255)
                                     gagaAAAATAATTCATATCTATCCACATGAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTa                                                                                                                  <  2:1639665/150‑1 (MQ=255)
                                      agaAAAATAATTCATATCTATCTACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTAt                                                                                                                 <  2:251199/150‑1 (MQ=255)
                                      agaAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTAt                                                                                                                 <  1:856886/150‑1 (MQ=255)
                                      agaAAAATAATTCATATATATCTACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACATAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTAt                                                                                                                 <  2:25241/150‑1 (MQ=255)
                                            ataatTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACaa                                                                                                           <  2:694648/150‑1 (MQ=255)
                                             taatTCATATCTATCCACATGAGAAAAAATCCCATTATCTCTATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACaaa                                                                                                          <  1:2360535/150‑1 (MQ=255)
                                                ttCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGtt                                                                                                       >  2:532471/1‑150 (MQ=255)
                                                  cATATCTATCCACATTAGAAAAAATCCCATTATCTCACTTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTAt                                                                                                     <  2:2266825/150‑1 (MQ=255)
                                                   atatCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATc                                                                                                    >  1:1883180/1‑150 (MQ=255)
                                                     atctatcCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCca                                                                                                  <  2:260996/150‑1 (MQ=255)
                                                          tcCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGa                                                                                             >  1:2302345/1‑150 (MQ=255)
                                                               aTTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGAtt                                                                                        >  2:2438453/1‑150 (MQ=255)
                                                                  aGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTgc                                                                                     <  2:1680974/150‑1 (MQ=255)
                                                                  aGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTgc                                                                                     <  1:2380992/150‑1 (MQ=255)
                                                                    aaaaaaTCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTgcga                                                                                   >  2:1015066/1‑150 (MQ=255)
                                                                     aaaaaTCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTgcgag                                                                                  <  2:28055/150‑1 (MQ=255)
                                                                     aaaaaTCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTgcgag                                                                                  >  1:212845/1‑150 (MQ=255)
                                                                     aaaaaTCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTgcgag                                                                                  >  2:699707/1‑150 (MQ=255)
                                                                     aaaaaTCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTgcgag                                                                                  >  1:1660296/1‑150 (MQ=255)
                                                                          tCCCAGTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGCCAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATcc                                                                            <  2:1094209/150‑1 (MQ=255)
                                                                               ttATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCagaa                                                                        <  1:614240/150‑1 (MQ=255)
                                                                                   ctcAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATc                                                                    >  1:943371/1‑150 (MQ=255)
                                                                                   ctcAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATc                                                                    <  1:1160610/150‑1 (MQ=255)
                                                                                    tcAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCt                                                                   <  1:1803606/150‑1 (MQ=255)
                                                                                    tcAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCt                                                                   >  2:2012084/1‑150 (MQ=255)
                                                                                    tcAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCt                                                                   >  1:222953/1‑150 (MQ=255)
                                                                                    tcAATTATTAGGGAAGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGGTAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCt                                                                   >  2:2078545/1‑150 (MQ=255)
                                                                                      aaTTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTAc                                                                 <  1:282606/150‑1 (MQ=255)
                                                                                         aattaGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAAATAAAACCGATCTAAATAAAATATGCACAAAGTTAACCACATGACGATTTGCGAGCGAACCAGAAGAACTACaaa                                                              <  2:1595133/149‑1 (MQ=255)
                                                                                          attaGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACaaaa                                                             <  2:951587/150‑1 (MQ=255)
                                                                                           ttaGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAg                                                            >  1:2462997/1‑150 (MQ=255)
                                                                                             aGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGAt                                                          >  1:1816733/1‑150 (MQ=255)
                                                                                                     atttatttTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGa                                                  >  1:2694993/1‑150 (MQ=255)
                                                                                                          tttttAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGc                                             >  1:1255731/1‑150 (MQ=255)
                                                                                                          tttttAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGc                                             >  1:1255979/1‑150 (MQ=255)
                                                                                                          tttttAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGc                                             <  1:1717950/150‑1 (MQ=255)
                                                                                                          tttttAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGc                                             >  1:2602568/1‑150 (MQ=255)
                                                                                                            tttAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCgg                                           <  1:890462/150‑1 (MQ=255)
                                                                                                            tttAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCgg                                           <  1:2074039/150‑1 (MQ=255)
                                                                                                             ttAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGt                                          <  1:40781/150‑1 (MQ=255)
                                                                                                                aCTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGaa                                       >  1:1190939/1‑150 (MQ=255)
                                                                                                                 cTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGaaa                                      >  1:587237/1‑150 (MQ=255)
                                                                                                                  tGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGaaaa                                     >  2:240574/1‑150 (MQ=255)
                                                                                                                  tGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGaaaa                                     >  2:1159265/1‑150 (MQ=255)
                                                                                                                    cATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAAc                                   >  2:272418/1‑150 (MQ=255)
                                                                                                                      tGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTAc                                                                 <  2:168196/118‑1 (MQ=255)
                                                                                                                      tGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTAc                                                                 >  1:168196/1‑118 (MQ=255)
                                                                                                                        aaaaaCAAAGACAAACATCCTGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCAt                               <  1:875512/150‑1 (MQ=255)
                                                                                                                        aaaaaCAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCAt                               >  1:82262/1‑150 (MQ=255)
                                                                                                                        aaaaaCAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCAt                               >  1:1329941/1‑150 (MQ=255)
                                                                                                                        aaaaaCAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCAt                               >  2:373893/1‑150 (MQ=255)
                                                                                                                        aaaaaCAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCAt                               >  1:31526/1‑150 (MQ=255)
                                                                                                                           aaCAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGtt                            >  2:436876/1‑150 (MQ=255)
                                                                                                                             cAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGtttt                          >  2:1154781/1‑150 (MQ=255)
                                                                                                                               aaGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCa                        <  1:1410343/150‑1 (MQ=255)
                                                                                                                                   cAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCct                    <  1:532471/150‑1 (MQ=255)
                                                                                                                                   cAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCct                    <  1:1588166/150‑1 (MQ=255)
                                                                                                                                    aaaCATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCctg                   >  2:1428107/1‑150 (MQ=255)
                                                                                                                                    aaaCATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCctg                   >  1:2438355/1‑150 (MQ=255)
                                                                                                                                    aaaCATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATATTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCctg                   >  2:2175588/1‑150 (MQ=255)
                                                                                                                                      ttatcatGCTTTTTACAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCctgtc                 >  1:2551791/3‑150 (MQ=255)
                                                                                                                                      ttatcatGCTTTTTACAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGAGTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTGTCATCctgtc                 >  2:1695444/3‑150 (MQ=255)
                                                                                                                                         tcatGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCctgtctgt              <  1:1389536/150‑1 (MQ=255)
                                                                                                                                          catGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCtgtg             <  2:587237/150‑1 (MQ=255)
                                                                                                                                          catGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCtgtg             <  1:955377/150‑1 (MQ=255)
                                                                                                                                           atGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTgg            >  1:860506/1‑150 (MQ=255)
                                                                                                                                                    aaaGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTa   <  2:2120118/150‑1 (MQ=255)
                                                                                                                                                     aaGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTAc  <  2:1782447/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TAGCAGAAAACGACCCAAATAACGGATGATCCTTAAGGAGAAAAATAATTCATATCTATCCACATTAGAAAAAATCCCATTATCTCAATTATTAGGGATGGATTTATTTTTAACTGCATGAAAAACAAAGACAAACATCATGCTGTAAAAAGCATGATAATAAATTAAAAGCGATGTAAATAATTTATGCACAAAGTTATCCACATGACGATTTGCGAGCGATCCAGAAGATCTACAAAAGATTTTCACGAAAAGCGGTGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTAC  >  NC_000913/4046638‑4046936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: