Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 495,027 T→G 6.1% intergenic (+17/‑93) recR → / → htpG gap repair protein/protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913495,0270TG 6.1% 569.3 / 23.8 163intergenic (+17/‑93)recR/htpGgap repair protein/protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase
Reads supporting (aligned to +/- strand):  ref base T (91/61);  new base G (5/5);  total (97/66)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

Only 100 of 163 total aligned reads displayed.

GAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCT  >  NC_000913/494879‑495175
                                                                                                                                                    |                                                                                                                                                    
gAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATc                                                                                                                                                     >  1:489664/1‑150 (MQ=255)
       gcgcgcAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGct                                                                                                                                              <  1:1906189/150‑1 (MQ=255)
       gcgcgcAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGct                                                                                                                                              <  1:2574989/150‑1 (MQ=255)
         gcgcAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGctct                                                                                                                                            <  2:2195223/150‑1 (MQ=255)
            cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGc                                                                                                                                         <  1:1310330/150‑1 (MQ=255)
            cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGc                                                                                                                                         <  1:1733886/150‑1 (MQ=255)
            cAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGc                                                                                                                                         <  1:2648382/150‑1 (MQ=255)
             aaTATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGTCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCt                                                                                                                                        <  1:1639237/150‑1 (MQ=255)
             aaTATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAGCGAGAGCAGGTGATCCTGCTCTCgtt                                                                                                                                        <  1:1184523/150‑3 (MQ=255)
             aaTATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAGCGAGAGCAGGTGATCCTGCTCTCgtt                                                                                                                                        <  1:1184511/150‑3 (MQ=255)
              atatGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGGTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCACGCGAGAGCAGGTGATCCTGCTCTCGttt                                                                                                                                       <  2:1854733/150‑1 (MQ=255)
                atGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGa                                                                                                                                     >  2:2586021/1‑150 (MQ=255)
                  gACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCGTGaaa                                                                                                                                   >  1:2351986/1‑150 (MQ=255)
                      tGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGTAattat                                                                                                                               >  1:2545192/1‑150 (MQ=255)
                      tGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAattat                                                                                                                               >  1:1948966/1‑150 (MQ=255)
                      tGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAattat                                                                                                                               >  1:358568/1‑150 (MQ=255)
                      tGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAattat                                                                                                                               >  1:358477/1‑150 (MQ=255)
                      tGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAattat                                                                                                                               >  1:1302897/1‑150 (MQ=255)
                      tGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCATGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTAGCTTGAAattat                                                                                                                               >  1:2309610/1‑150 (MQ=255)
                        gAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGTGCAGGATCACCTGCTCTCGCTTGAAATTATtc                                                                                                                             >  2:470890/1‑150 (MQ=255)
                           gccagccGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTcc                                                                                                                          <  2:331670/150‑1 (MQ=255)
                             cagccGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCt                                                                                                                        >  2:2389497/1‑150 (MQ=255)
                              agccGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCtt                                                                                                                       >  2:1067892/1‑150 (MQ=255)
                              agccGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCtt                                                                                                                       >  1:2658338/1‑150 (MQ=255)
                              agccGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCtt                                                                                                                       >  2:2564481/1‑150 (MQ=255)
                              agccGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCtt                                                                                                                       >  1:2049787/1‑150 (MQ=255)
                               gccGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTg                                                                                                                      >  1:1708258/1‑150 (MQ=255)
                                        cTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCAtct                                                                                                             <  2:2586331/150‑1 (MQ=255)
                                         tCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCAtctc                                                                                                            >  2:1077703/1‑150 (MQ=255)
                                           aTGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCAtctctc                                                                                                          >  2:1877384/1‑150 (MQ=255)
                                            tGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTcc                                                                                                         >  1:2312621/1‑150 (MQ=255)
                                              gCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCca                                                                                                       >  2:2387608/1‑150 (MQ=255)
                                              gCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCGTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTAGAAATTATTCTCCCATGTCCCCATCTCTCCca                                                                                                       <  2:2177748/150‑1 (MQ=255)
                                               cGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCcac                                                                                                      >  2:1209226/1‑150 (MQ=255)
                                               cGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCcac                                                                                                      >  1:802665/1‑150 (MQ=255)
                                                 ttCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCCCAt                                                                                                    >  1:88911/1‑150 (MQ=255)
                                                  tCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATc                                                                                                   <  1:2026319/150‑1 (MQ=255)
                                                  tCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATc                                                                                                   <  1:392944/150‑1 (MQ=255)
                                                    cGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCt                                                                                                 >  1:821423/1‑150 (MQ=255)
                                                    cGGTTGGCGGCGAGCTGGAAATGGTCGACGGCAACACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAACTTATTCTCCCTGGTCCCCATCTCTCCCACATCCt                                                                                                 >  2:1491618/1‑150 (MQ=255)
                                                      gTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGt                                                                                               >  1:1116899/1‑150 (MQ=255)
                                                      gTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGt                                                                                               >  1:1252718/1‑150 (MQ=255)
                                                      gTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGt                                                                                               >  1:1252726/1‑150 (MQ=255)
                                                          gcggcgAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGttttt                                                                                           >  1:699242/1‑150 (MQ=255)
                                                            ggcgAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTaa                                                                                         <  1:1586244/150‑1 (MQ=255)
                                                              cgAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAAcc                                                                                       >  2:1567157/1‑150 (MQ=255)
                                                                    ggAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCAc                                                                                                                                                   <  1:1631545/84‑1 (MQ=255)
                                                                     gAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAAt                                                                                >  1:1790478/1‑150 (MQ=255)
                                                                        aTGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGc                                                                             <  2:2272506/150‑1 (MQ=255)
                                                                           gTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCatt                                                                          >  1:277493/1‑150 (MQ=255)
                                                                           gTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCatt                                                                          <  1:2473498/150‑1 (MQ=255)
                                                                                cGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGa                                                                     <  1:1094591/150‑1 (MQ=255)
                                                                                 ggCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAg                                                                    <  1:14487/150‑1 (MQ=255)
                                                                                    accacGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTa                                                                 <  2:627091/150‑1 (MQ=255)
                                                                                    accacGGTGTCACACTCCCTGGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAATTTATTCTCCCTTGTCCCCATCTCTCCCCCATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTa                                                                 <  2:1574703/150‑1 (MQ=255)
                                                                                       acGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGAc                                                              >  1:2748301/1‑150 (MQ=255)
                                                                                       acGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGAc                                                              <  1:2564481/150‑1 (MQ=255)
                                                                                       acGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGAc                                                              >  2:1642129/1‑150 (MQ=255)
                                                                                         gTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCt                                                            >  1:2448020/1‑150 (MQ=255)
                                                                                               acacTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGa                                                      >  2:2047864/1‑150 (MQ=255)
                                                                                               acacTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGa                                                      >  2:1629907/1‑150 (MQ=255)
                                                                                                cacTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGaa                                                     <  1:2232986/150‑1 (MQ=255)
                                                                                                cacTCCCGTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGaa                                                     <  1:2232997/150‑1 (MQ=255)
                                                                                                 acTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGaaa                                                    >  2:2717021/1‑150 (MQ=255)
                                                                                                 acTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGaaa                                                    >  2:1579368/1‑150 (MQ=255)
                                                                                                       ttGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACaa                                              <  2:294462/150‑1 (MQ=255)
                                                                                                       ttGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACaa                                              <  2:2228978/150‑1 (MQ=255)
                                                                                                       ttGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACaa                                              <  1:306277/150‑1 (MQ=255)
                                                                                                         gCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGa                                            <  1:2444988/150‑1 (MQ=255)
                                                                                                              gCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTc                                       <  2:151700/150‑1 (MQ=255)
                                                                                                              gCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTc                                       <  2:2632505/150‑1 (MQ=255)
                                                                                                                            gTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCCTTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTtcagtca                         >  2:401395/1‑150 (MQ=255)
                                                                                                                             tttttAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTtcagtcag                        >  1:53890/1‑150 (MQ=255)
                                                                                                                             tttttAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTACTGAGGTAGACCTACATGAAAGGACAAGAAACTCGt                                     <  1:626763/137‑1 (MQ=255)
                                                                                                                             tttttAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACAGCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTtcagtcag                        >  2:2344110/1‑150 (MQ=255)
                                                                                                                              ttttAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGa                       <  2:489664/150‑1 (MQ=255)
                                                                                                                                ttAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAg                     <  1:633650/150‑1 (MQ=255)
                                                                                                                                ttAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAg                     >  2:801819/1‑150 (MQ=255)
                                                                                                                                  aaGCAAACTAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTg                   <  1:915855/150‑1 (MQ=255)
                                                                                                                                  aaGCAAACGAGAGAAGGATCAACTGCTCTCGCTTGAAATTATTCTCCCATGTCCCCATCTCTCACACATCCTGTATTTAACCATAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACACGTGGTTTTAAGTCAGAAGtt                   >  2:656437/1‑149 (MQ=255)
                                                                                                                                   aGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGACACTCGTGGTTTTCAGTCAGAAGTGa                  >  2:1952119/1‑150 (MQ=255)
                                                                                                                                   aGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGa                  >  2:380100/1‑150 (MQ=255)
                                                                                                                                    gCAATCGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGTGGTAGACCTACATGAAAGGACTAGATACTCGTGGTTTTCTGTCAGAAGTgta                 >  2:2510392/1‑148 (MQ=255)
                                                                                                                                    gCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGaa                 >  2:1001628/1‑150 (MQ=255)
                                                                                                                                     cAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGaaa                >  2:2455181/1‑150 (MQ=255)
                                                                                                                                      aagcGAGAGCAGGTGATCCTGCTCTCGTTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAAc               >  1:1536914/4‑150 (MQ=255)
                                                                                                                                      aagcGAGAGCAGGTGATCCTGCTCTCGTTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAAc               >  1:2123935/4‑150 (MQ=255)
                                                                                                                                      aaaCGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAAc               >  1:1424467/1‑150 (MQ=255)
                                                                                                                                       agcGAGAGCAGGTGATCCTGCTCTCGTTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACa              >  2:1181714/3‑150 (MQ=255)
                                                                                                                                       aaCGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACa              >  2:1164010/1‑150 (MQ=255)
                                                                                                                                        aCGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGaa                      >  2:116488/1‑141 (MQ=255)
                                                                                                                                        aCGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGaa                      <  1:116488/141‑1 (MQ=255)
                                                                                                                                        aCGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAg             >  2:563925/1‑150 (MQ=255)
                                                                                                                                         cGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGc            <  2:2312621/150‑1 (MQ=255)
                                                                                                                                          gagaGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCt           >  2:1238732/1‑150 (MQ=255)
                                                                                                                                           agagCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGTACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCtt          <  1:922646/150‑1 (MQ=255)
                                                                                                                                           agagCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCtt          >  1:1627380/1‑150 (MQ=255)
                                                                                                                                              gCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACCAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTg       >  2:1628565/1‑150 (MQ=255)
                                                                                                                                              gCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTg       >  2:1386943/1‑150 (MQ=255)
                                                                                                                                                  gATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCAcc   >  2:201213/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAGCTTTGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTTCCGGTTGGCGGCGAGCTGGAAATGGTCGACGGCACCACGTTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCT  >  NC_000913/494879‑495175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: