Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,314,297 T→A 5.2% intergenic (+100/+47) yjdP → / ← phnP putative periplasmic protein/5‑phospho‑alpha‑D‑ribosyl 1,2‑cyclic phosphate phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,314,2970TA 5.2% 556.5 / 12.7 160intergenic (+100/+47)yjdP/phnPputative periplasmic protein/5‑phospho‑alpha‑D‑ribosyl 1,2‑cyclic phosphate phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base T (62/88);  new base A (5/3);  total (68/92)
Fisher's exact test for biased strand distribution p-value = 2.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 167 total aligned reads displayed.

GGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGCCACGCATCAAACTGGTGGCTGATATGGGTCA  >  NC_000913/4314148‑4314444
                                                                                                                                                     |                                                                                                                                                   
  cAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTgg                                                                                                                                                   >  2:953971/1‑150 (MQ=255)
  cAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTgg                                                                                                                                                   <  1:678740/150‑1 (MQ=255)
  cAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTgg                                                                                                                                                   <  1:1622802/150‑1 (MQ=255)
  cAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTgg                                                                                                                                                   <  2:2061344/150‑1 (MQ=255)
     cTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGga                                                                                                                                                <  2:1730992/150‑1 (MQ=255)
     cTGGAGGAGGAAGAGCGGAGAAGGGAAGATGAGTCTGGGCGATGAGGTGGGGTATGGAGCAGGCATCGCCCCTCACCCCCATGGGGCGAGGGAGAAACCGCGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGga                                                                                                                                                <  2:1028375/150‑1 (MQ=255)
      tGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGgag                                                                                                                                               >  1:904075/1‑150 (MQ=255)
       ggaggaTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGgaga                                                                                                                                              >  1:1545633/1‑150 (MQ=255)
        gaggaTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGagag                                                                                                                                             <  1:1125050/150‑1 (MQ=255)
        gaggaTGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGagag                                                                                                                                             <  2:2415553/150‑1 (MQ=255)
                   gCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGa                                                                                                                                  >  2:1630319/1‑150 (MQ=255)
                   gCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGa                                                                                                                                  >  2:1801769/1‑150 (MQ=255)
                    cGGGGAATGGAAGATGTGTATGGGCGATGAGGTTGGGTATGGAGCTGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                                                                                                 >  1:676538/1‑150 (MQ=255)
                     ggAGAATTGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                                                                                                <  2:1517222/150‑1 (MQ=255)
                     ggAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                                                                                                <  1:2596161/150‑1 (MQ=255)
                       agaATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgggg                                                                                                                              >  1:2381986/1‑150 (MQ=255)
                       agaATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgggg                                                                                                                              >  1:1736207/1‑150 (MQ=255)
                        gaATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAggggg                                                                                                                             <  2:601309/150‑1 (MQ=255)
                        gaATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAggggg                                                                                                                             <  2:637923/150‑1 (MQ=255)
                           tGGAAGATGAGTAGGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCCCTGGGGAGAGGGAGAAAACGAGCGCAAGATTCAATATCGAGCACAATCGGGCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCa                                                                                                                          <  2:1153003/150‑1 (MQ=39)
                              aaGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAgcg                                                                                                                       >  2:1230445/1‑150 (MQ=255)
                                       aTGGGCGTTGTGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTAAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGTGGGTTTGGGTGAGGGGGCCAGCGCGCACAGTc                                                                                                              <  1:322146/150‑1 (MQ=39)
                                       aTGGGCGTTGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGTGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTc                                                                                                              <  1:693373/150‑1 (MQ=255)
                                        tGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTcc                                                                                                             >  2:2149342/1‑150 (MQ=255)
                                        tGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTcc                                                                                                             >  2:2149269/1‑150 (MQ=255)
                                         gggCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCACTATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCCGCGCGCACATTccc                                                                                                            >  1:2219592/1‑150 (MQ=255)
                                          ggCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCACTATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCct                                                                                                           >  2:2060890/1‑150 (MQ=255)
                                          ggCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCct                                                                                                           <  2:1110533/150‑1 (MQ=255)
                                          ggCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCct                                                                                                           >  2:2114536/1‑150 (MQ=255)
                                           gCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCctc                                                                                                          >  2:2113224/1‑150 (MQ=255)
                                           gCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCctc                                                                                                          <  2:1583220/150‑1 (MQ=255)
                                             gATGAGGTTGGTTAGGGAGCAGGCATCGCCCCTCACCCCAATGGGGTGTGGGAGAAAACGCGCGCAATATTCAATATCGAGCACAATCGGTCCCCGCGCCCCTCTGGGGAGTGGGTTAGGGTGTGGGGGCCAGCGCGCACATTCCCtctc                                                                                                        <  1:1262068/150‑1 (MQ=255)
                                             gATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCtctc                                                                                                        <  2:281782/150‑1 (MQ=255)
                                              aTGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCtca                                                                                                       >  2:2232641/1‑150 (MQ=255)
                                               tGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCtcat                                                                                                      <  1:1090744/150‑1 (MQ=255)
                                               tGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCtcat                                                                                                      <  1:2605372/150‑1 (MQ=255)
                                               tGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCtcat                                                                                                      <  1:1915274/150‑1 (MQ=255)
                                                             gcgCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGTGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAAt                                                                                        <  1:2396055/148‑1 (MQ=255)
                                                             gAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAAt                                                                                        <  1:2149342/150‑1 (MQ=255)
                                                             gAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAAt                                                                                        >  2:663350/1‑150 (MQ=255)
                                                             gAGCAGGCATCGCACCTCACCCCATTGGGTAGTGGGATAAAACGAGCGAAATATTAAATATCGAGCAAAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAAt                                                                                        <  1:1249517/150‑1 (MQ=38)
                                                                cAGGCATCGCCCCTCACCCCAAGGGGGAGAGGGAGACAACGAGCGCACTAGTCAATATCGAGCACAATCGGTCCCCTCGCCCCGCGGGGGAGCGGGGGAGGGTGAGGGGGCCCGCGCGCACATTCCCGCGCCTCACGCCACCCCaagcgc                                                                                     >  2:1395236/1‑146 (MQ=255)
                                                                   gCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTccat                                                                                  >  1:2230423/1‑150 (MQ=255)
                                                                   gCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTccat                                                                                  <  1:1753083/150‑1 (MQ=255)
                                                                      tCGCCCCTCACCCCACTGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGCGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTccatccc                                                                               <  2:2275228/150‑1 (MQ=255)
                                                                      gcGCCCCTCACCCCAATGGGGAGAGGGAGACCACGAGCGCAAGATTCAATCTCGAGCACACTCGGTCCCCCCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTccatccc                                                                               <  2:1244490/149‑1 (MQ=39)
                                                                          cccTCTCCCCATTGGGGTGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCa                                                                           >  1:585502/1‑150 (MQ=255)
                                                                           ccTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCaa                                                                          <  2:2120792/150‑1 (MQ=255)
                                                                           ccTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCaa                                                                          >  1:726186/1‑150 (MQ=255)
                                                                             tCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAAc                                                                        >  1:2314682/1‑150 (MQ=255)
                                                                             tCACCCCAATGGCGAGAGGGAGAAAACGAGCGCAATATTCCATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGATAGGGTGAGGGGGCCCGCCCGCACATTCCCTCTCAACACGCCACCCCAATCTCCATCCCATCAAAc                                                                        >  2:2653988/1‑150 (MQ=38)
                                                                               aCCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCCGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAAccc                                                                      >  2:744000/1‑150 (MQ=255)
                                                                               aCCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAAccc                                                                      <  2:2279369/150‑1 (MQ=255)
                                                                               aCCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAAccc                                                                      <  2:2652945/150‑1 (MQ=255)
                                                                               aCCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAAccc                                                                      <  1:1041571/150‑1 (MQ=255)
                                                                                    aaTGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCt                                                                 <  2:1545633/150‑1 (MQ=255)
                                                                                         ggagagggagaAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAAc                                                            <  1:1665420/150‑1 (MQ=255)
                                                                                          gagagggagaAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGTGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAAcc                                                           <  1:1395181/150‑1 (MQ=255)
                                                                                           agagggagaAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAAccc                                                          <  1:1051953/150‑1 (MQ=255)
                                                                                               ggagaAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGAc                                                      <  1:1728648/150‑1 (MQ=255)
                                                                                               ggagaAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGAc                                                      >  2:1968472/1‑150 (MQ=255)
                                                                                               ggagaAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCACCATCCCATCAAACCCCACCTCAAACCCTGAc                                                      >  2:615396/1‑150 (MQ=255)
                                                                                                gagaAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACg                                                     >  2:1769069/1‑150 (MQ=255)
                                                                                                   aaaaCGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTa                                                  >  2:1448961/1‑150 (MQ=255)
                                                                                                    aaaCGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAg                                                 <  1:953971/150‑1 (MQ=255)
                                                                                                     aaCGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGt                                                <  1:2514638/150‑1 (MQ=255)
                                                                                                        gAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCg                                             >  1:1462210/1‑150 (MQ=255)
                                                                                                        gAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCg                                             >  1:2085995/1‑150 (MQ=255)
                                                                                                           cgcAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGttt                                          >  1:1164254/1‑150 (MQ=255)
                                                                                                               ctagtCAATATCGCGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCa                                      <  1:1278632/146‑1 (MQ=255)
                                                                                                               atatTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCa                                      <  1:789139/150‑1 (MQ=255)
                                                                                                                tatTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCAt                                     <  1:1143196/150‑1 (MQ=255)
                                                                                                                tatTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCAt                                     <  1:581683/150‑1 (MQ=255)
                                                                                                                  ttCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCa                                   <  1:2060890/150‑1 (MQ=255)
                                                                                                                  ttCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCa                                   <  1:259743/150‑1 (MQ=255)
                                                                                                                    cAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTCGTGCGTTTTCCATCCGc                                 >  2:239282/1‑150 (MQ=255)
                                                                                                                    cAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGc                                 >  2:1837850/1‑150 (MQ=255)
                                                                                                                       gctCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGCCAc                              <  2:146067/148‑1 (MQ=255)
                                                                                                                         tCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGCCACGc                            >  1:1598552/1‑150 (MQ=255)
                                                                                                                         tCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACAGTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGCCACGc                            <  1:2632026/150‑1 (MQ=255)
                                                                                                                          cGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGCCACGCa                           <  2:928094/150‑1 (MQ=255)
                                                                                                                             gCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGCCACGCATCa                        >  2:723231/1‑150 (MQ=255)
                                                                                                                             gCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTACATCAGCCACGCATCa                        >  2:2482899/1‑150 (MQ=255)
                                                                                                                                   tCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGCCACGCATCAAACtgg                  <  2:2096614/150‑1 (MQ=255)
                                                                                                                                    cctaacccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGc                                 <  2:679970/129‑1 (MQ=255)
                                                                                                                                    cctaacccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGc                                 >  1:679970/6‑134 (MQ=255)
                                                                                                                                      taacccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGGCCCGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCACAAACCCTGACGGTAGTGCGTTTTCCATCAGCCACGCATCAAACtggtgg               >  1:759652/4‑150 (MQ=255)
                                                                                                                                      taacccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGCCACGCATCAAACtggtgg               >  1:2134005/4‑150 (MQ=255)
                                                                                                                                      ggccccTCGCCCCTCTGGGGAGAGGGTTCGGGTGCGGGGGCCAGCGCGCACCTTCCCTCTCATCACGCCACCCCACTCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGCCACGCATCAAACtggtgg               <  1:2113224/148‑1 (MQ=255)
                                                                                                                                      gTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGCCACGCATCAAACtggtgg               <  1:2724013/150‑1 (MQ=255)
                                                                                                                                      gTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGCCACGCATCAAACtggtgg               <  1:386867/150‑1 (MQ=255)
                                                                                                                                         cccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGCCACGCATCAAACTGGTGGCTg            >  1:87180/1‑150 (MQ=255)
                                                                                                                                          ccTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGCCACGCATCAAACTGGTGGCTGa           <  1:2091006/150‑1 (MQ=255)
                                                                                                                                          ccTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGCCACGCATCAAACTGGTGGCTGa           <  1:2091086/150‑1 (MQ=255)
                                                                                                                                           cTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGCCACGCATCAAACTGGTGGCTGat          >  1:1970952/1‑150 (MQ=255)
                                                                                                                                                cccTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGCCACGCATCAAACTGGTGGCTGATATggg     >  1:585974/1‑150 (MQ=255)
                                                                                                                                                  ctctGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGCCACGCATCAAACTGGTGGCTGATATGGGTc   >  2:1784826/1‑150 (MQ=255)
                                                                                                                                                   tctTGGTAGAGGTTTAGGGAGACCGGGACATCGCTCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATAAAACCCCAACTCAAACCATTACCCTAGATCGTAATCCATCAGCCACGCATAAAACTTTTGTCAGATAAGTGTCa  >  2:2676672/1‑150 (MQ=255)
                                                                                                                                                   tctGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGCCACGCATCAAACTGGTGGCTGATATGGGTCa  >  2:800536/1‑150 (MQ=255)
                                                                                                                                                   tctGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGCCACGCATCAAACTGGTGGCTGATATGGGTCa  >  1:434372/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GGCAACTGGAGGATGAAGAGCGGAGAATGGAAGATGAGTATGGGCGATGAGGTTGGGTATGGAGCAGGCATCGCCCCTCACCCCAATGGGGAGAGGGAGAAAACGAGCGCAATATTCAATATCGAGCACAATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGGCCAGCGCGCACATTCCCTCTCATCACGCCACCCCAATCTCCATCCCATCAAACCCCACCTCAAACCCTGACGGTAGTGCGTTTTCCATCAGCCACGCATCAAACTGGTGGCTGATATGGGTCA  >  NC_000913/4314148‑4314444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: