Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,614,321 A→G 6.4% intergenic (+61/+359) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,614,3210AG 6.4% 666.5 / 15.1 216intergenic (+61/+359)yjjV/yjjWputative DNase/putative pyruvate formate lyase activating enzyme
Reads supporting (aligned to +/- strand):  ref base A (74/125);  new base G (9/5);  total (86/130)
Fisher's exact test for biased strand distribution p-value = 5.19e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

CCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATA  >  NC_000913/4614172‑4614470
                                                                                                                                                     |                                                                                                                                                     
ccGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTa                                                                                                                                                       >  1:1063625/1‑150 (MQ=255)
    gCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAcgc                                                                                                                                                   <  2:1128053/150‑1 (MQ=255)
    gCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAcgc                                                                                                                                                   <  1:2280059/150‑1 (MQ=255)
    gCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAcgc                                                                                                                                                   >  1:300008/1‑150 (MQ=255)
    gCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAcgc                                                                                                                                                   <  1:1066248/150‑1 (MQ=255)
    gCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAcgc                                                                                                                                                   <  1:1066369/150‑1 (MQ=255)
    gCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTCTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAAcgc                                                                                                                                                   >  1:1652847/1‑150 (MQ=255)
          cGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTa                                                                                                                                             >  1:263516/1‑150 (MQ=255)
          cGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAAAAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTa                                                                                                                                             >  1:36449/1‑150 (MQ=255)
              ttGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCAt                                                                                                                                         >  1:594483/1‑150 (MQ=255)
                gcgcCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGc                                                                                                                                       <  2:2175706/150‑1 (MQ=255)
                 cgcCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGcc                                                                                                                                      >  2:2184727/1‑150 (MQ=255)
                  gcCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGATTAATAACACGTATACGTTGTTTAAAGTGCCGTATGCCGGATAAGGCGTTAACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGAAGCTTAACGCGTCTTATCATGcca                                                                                                                                     >  1:1255971/1‑149 (MQ=25)
                    cGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTAc                                                                                                                                   <  1:475531/150‑1 (MQ=255)
                      ggAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAg                                                                                                                                 <  1:2163224/150‑1 (MQ=255)
                        aaCCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGt                                                                                                                               <  1:487550/150‑1 (MQ=255)
                            ggcggATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTtgt                                                                                                                           >  1:1012876/1‑150 (MQ=255)
                              cggATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGc                                                                                                                         <  2:497051/150‑1 (MQ=255)
                                 aTGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGa                                                                                                                      >  2:1073839/1‑150 (MQ=255)
                                  tGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGaa                                                                                                                     <  2:374881/150‑1 (MQ=255)
                                   gagaTTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAAc                                                                                                                    >  1:1253642/1‑150 (MQ=255)
                                   gagaTTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAAc                                                                                                                    <  1:1499312/150‑1 (MQ=255)
                                    agaTTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAAcc                                                                                                                   <  1:1922268/150‑1 (MQ=255)
                                     gaTTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCg                                                                                                                  <  2:1324709/150‑1 (MQ=255)
                                      aTTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGt                                                                                                                 >  1:2725600/1‑150 (MQ=255)
                                          cgcAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGAc                                                                                                                                                     >  1:1358127/1‑110 (MQ=255)
                                          cgcAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGc                                                                                                             <  1:91754/150‑1 (MQ=255)
                                           gcAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGTCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCATATCATGCCTACAGGTTTGTGCCGAACCGTAGGcc                                                                                                            <  2:2083082/150‑1 (MQ=255)
                                           gcAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGcc                                                                                                            >  2:1040892/1‑150 (MQ=255)
                                           gcAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCGTATCATGCCTACAGGTTTGTGCCGAACCGGAGGcc                                                                                                            >  2:882683/1‑150 (MQ=255)
                                              aGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGa                                                                                                         <  1:1485798/150‑1 (MQ=255)
                                                 gTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATaa                                                                                                      <  1:460417/150‑1 (MQ=255)
                                                 gTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATaa                                                                                                      <  1:1163575/150‑1 (MQ=255)
                                                  ttGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAg                                                                                                     >  2:1943989/1‑150 (MQ=255)
                                                    gCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGc                                                                                                   <  1:1621947/150‑1 (MQ=255)
                                                    gCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGc                                                                                                   <  1:1726396/150‑1 (MQ=255)
                                                       taataaCACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTAGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCATGCCTACAGGATTCTGCCGAACCGTAGGCCGGATAAGGCGtt                                                                                                >  1:1570455/1‑150 (MQ=255)
                                                        aataaCACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTc                                                                                               >  1:314647/1‑150 (MQ=255)
                                                           aaCACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcg                                                                                            <  2:363340/150‑1 (MQ=255)
                                                              acGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTACACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTAAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATGCGGCGTGAAcgcct                                                                                         <  2:1418676/150‑2 (MQ=17)
                                                                  ataCGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATc                                                                                     >  1:2181840/1‑150 (MQ=255)
                                                                  ataCGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGACGAACCGTAGGCCGGATAAGCAGTACTCGCCCCATc                                                                                     >  1:808377/1‑150 (MQ=17)
                                                                  ataCGGTGTTTAACGTGCCGTAGGCCGGATAAGGCGTACACGCCGCATCCGGCAGTTGGCGCACACTGCCTGATGCGACGCTGAACGCGTCTTATCATGCCCACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATc                                                                                     <  2:1520472/150‑1 (MQ=17)
                                                                    aCGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                                                   <  2:2133698/150‑1 (MQ=255)
                                                                      gttgttTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                 >  2:38195/1‑150 (MQ=255)
                                                                      gttgttTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                 >  2:2126934/1‑150 (MQ=255)
                                                                       ttgttTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                                                <  1:1786669/150‑1 (MQ=255)
                                                                       ttgttTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                                                >  1:561032/1‑150 (MQ=255)
                                                                        tgttTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcgccg                                                                                         >  1:172067/1‑140 (MQ=255)
                                                                          tttAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGATGGCGCACAATGCATGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGtg                                                                             >  1:2365722/1‑149 (MQ=255)
                                                                            tAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTgg                                                                           <  1:947500/150‑1 (MQ=255)
                                                                                 tGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGATCTCGCCGCATCCGGCAGTTGGCGcac                                                                      >  1:1495707/1‑150 (MQ=255)
                                                                                 tGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTGGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGcac                                                                      >  2:876867/1‑150 (MQ=9)
                                                                                    cGTAGGCCGGATAAGGCGTTCACGCCGCCTCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAAt                                                                   <  2:769732/150‑1 (MQ=255)
                                                                                     gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATg                                                                  <  2:1199241/150‑1 (MQ=37)
                                                                                     gTAGGCCGGATAAGCCGATCACGCCGCAACCGGCAGATGGCGCACAATGCCTGAAGCGACGCTTAACGCGTCTTAACATGCCAACAGCTTTCTCCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATg                                                                  <  2:2232030/150‑1 (MQ=14)
                                                                                      tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGc                                                                 <  2:594483/150‑1 (MQ=255)
                                                                                       aGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGcc                                                                <  1:868961/150‑1 (MQ=255)
                                                                                        ggCCGGATAAGGCGTTCACGCCGCCTCCGGCAGTTGGCGCACACTGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCt                                                               <  2:1729244/150‑1 (MQ=255)
                                                                                        ggCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACACTGCCTGATGCGACGCTTAACGCGTCTTATCACGCCTACCGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGccg                                                               <  2:2157212/150‑2 (MQ=255)
                                                                                        ggCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTGGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGATGGCGCACAATGCCt                                                               >  2:867117/1‑150 (MQ=255)
                                                                                          ccGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGa                                                             >  1:2585181/1‑150 (MQ=255)
                                                                                          caggATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGa                                                             <  1:2046475/148‑1 (MQ=255)
                                                                                           cGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGAt                                                            <  1:38195/150‑1 (MQ=255)
                                                                                            ggATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATg                                                           <  2:1270059/150‑1 (MQ=37)
                                                                                                 aGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACg                                                      <  2:1573910/150‑1 (MQ=255)
                                                                                                 aGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTGTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACg                                                      >  2:2050977/1‑150 (MQ=255)
                                                                                                   gCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCt                                                    >  1:1551606/1‑150 (MQ=255)
                                                                                                   gCGTTCACGCCGCATCCGGCAGTTGGCGCAAAATGCCTGAAGCGACGCTTAACGCGACTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCTCGCCGCATCCGGCAGTTGGCGCACGATGCCTGTTGCGACGcg                                                    >  1:1517162/1‑149 (MQ=11)
                                                                                                    cGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCtt                                                   <  2:175093/150‑1 (MQ=255)
                                                                                                        cACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTcg                                               <  1:2619160/150‑1 (MQ=255)
                                                                                                         aCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCATGCCTACAGGTATGTGCCGAACCGTGGGCCGTATAAGGCGTGCCCGCCGCATCCGGCCGTAGGCGCCCAAAGCCTGAGGCGGCGCTCGTcgc                                              >  1:689592/1‑150 (MQ=11)
                                                                                                                aTCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTAt                                       <  2:2725600/150‑1 (MQ=255)
                                                                                                                 tCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATc                                      <  2:2352784/150‑1 (MQ=255)
                                                                                                                    ggCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCATg                                   >  1:2569872/1‑150 (MQ=255)
                                                                                                                     gCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGc                                  <  2:34575/150‑1 (MQ=255)
                                                                                                                     gCAGTTGGCGCACAATGCCTGATGCGACGCTGAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGc                                  <  1:107888/150‑1 (MQ=255)
                                                                                                                     gCAGTTGGCGCACAATGACTTAAGCGACGCTTAACGCGTCTTATAATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTTGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGc                                  <  2:2353039/150‑1 (MQ=14)
                                                                                                                          tGGCGCACACGGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACa                             <  2:1705210/150‑1 (MQ=255)
                                                                                                                          tGGCGCACAATGCCTGATGCGACGCTTATCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCAGGCCTACa                             >  1:886394/1‑150 (MQ=2)
                                                                                                                          tGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACa                             >  2:652984/1‑150 (MQ=2)
                                                                                                                          tGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCTGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACa                             >  1:857116/1‑150 (MQ=255)
                                                                                                                            gcgcACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAg                           >  2:2620824/1‑150 (MQ=255)
                                                                                                                              gcACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTc                         >  2:756381/1‑150 (MQ=21)
                                                                                                                               cacaATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCt                        >  2:1464956/1‑150 (MQ=255)
                                                                                                                                 caATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAGTGGGCGCACAATGCCTGAGGCGACGCTTGTCGCGGCGTATCATGCCTCCAAGTCtgt                      >  2:2278766/1‑150 (MQ=14)
                                                                                                                                 caATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCtgt                      >  2:866278/1‑150 (MQ=255)
                                                                                                                                  aaTGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCtgtg                     <  1:1801399/150‑1 (MQ=255)
                                                                                                                                   ctGCCTGCTGCGACGCTTAACGCGTCTTATCAGGCCTCCAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGc                    <  2:2473538/149‑1 (MQ=255)
                                                                                                                                   aTGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGc                    <  1:1263251/150‑1 (MQ=255)
                                                                                                                                     gCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCg                  <  1:2127053/150‑1 (MQ=255)
                                                                                                                                       cTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGaa                <  2:252556/150‑1 (MQ=255)
                                                                                                                                        tGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAAc               >  2:1587100/1‑150 (MQ=255)
                                                                                                                                               aCGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGc        <  2:2585107/150‑1 (MQ=255)
                                                                                                                                                cGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGcc       >  1:2516799/1‑150 (MQ=255)
                                                                                                                                                 gCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCg      <  2:2367015/150‑1 (MQ=255)
                                                                                                                                                 gCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCg      >  2:1017027/1‑150 (MQ=255)
                                                                                                                                                   ttAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGa    >  2:2596269/1‑150 (MQ=255)
                                                                                                                                                   ttAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGa    <  2:81267/150‑1 (MQ=255)
                                                                                                                                                   ttAACGCGTCTTATCATGCCTACAGGTGTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGa    <  2:1772665/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATA  >  NC_000913/4614172‑4614470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: