Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,499,851 T→C 8.0% L394S (TTG→TCG)  ypdA → sensor kinase regulating yhjX; pyruvate‑responsive YpdAB two‑component system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,499,8510TC 8.0% 432.0 / 20.3 137L394S (TTG→TCG) ypdAsensor kinase regulating yhjX; pyruvate‑responsive YpdAB two‑component system
Reads supporting (aligned to +/- strand):  ref base T (46/80);  new base C (9/2);  total (55/82)
Fisher's exact test for biased strand distribution p-value = 7.11e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 142 total aligned reads displayed.

GGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGACGGTTATCTATGAT  >  NC_000913/2499702‑2499999
                                                                                                                                                     |                                                                                                                                                    
tgAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGtt                                                                                                                                                      <  1:1072088/149‑1 (MQ=255)
ggAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGtt                                                                                                                                                      <  1:1072074/150‑1 (MQ=255)
   ggTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGAt                                                                                                                                                   <  1:1757049/150‑1 (MQ=255)
     tttCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCt                                                                                                                                                 >  2:2195359/1‑150 (MQ=255)
     tttCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCt                                                                                                                                                 >  2:1181236/1‑150 (MQ=255)
     tttCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCt                                                                                                                                                 >  2:1456356/1‑150 (MQ=255)
     tttCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCt                                                                                                                                                 >  1:1410741/1‑150 (MQ=255)
        cACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTa                                                                                                                                              <  2:1841476/150‑1 (MQ=255)
         aCGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTaa                                                                                                                                             >  1:2243878/1‑150 (MQ=255)
         aCGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTaa                                                                                                                                             >  2:1246627/1‑150 (MQ=255)
         aCGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTaa                                                                                                                                             <  1:2594841/150‑1 (MQ=255)
         aCGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTaa                                                                                                                                             >  2:1246623/1‑150 (MQ=255)
         aCGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTaa                                                                                                                                             <  1:1959402/150‑1 (MQ=255)
         aCGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTaa                                                                                                                                             >  1:870480/1‑150 (MQ=255)
           gcgcCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATc                                                                                                                                           <  2:2674656/150‑1 (MQ=255)
             gcCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTg                                                                                                                                         >  2:437172/1‑150 (MQ=255)
                    agcTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGtt                                                                                                                                  <  2:1614018/150‑1 (MQ=255)
                    agcTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGtt                                                                                                                                  <  1:441365/150‑1 (MQ=255)
                          gTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTCGATCTTTAATCTGTCGCGTTATCTgc                                                                                                                            >  2:1071125/1‑150 (MQ=255)
                          gTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTCGATCTTTAATCTGTCGCGTTATCTgc                                                                                                                            >  2:1062983/1‑150 (MQ=255)
                          gTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTCGATCTTTAATCTGTCGCGTTATCTgc                                                                                                                            >  2:1071085/1‑150 (MQ=255)
                          gTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTCGATCTTTAATCTGTCGCGTTATCTgc                                                                                                                            >  2:1071080/1‑150 (MQ=255)
                          gTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTCGATCTTTAATCTGTCGCGTTATCTgc                                                                                                                            >  2:1650367/1‑150 (MQ=255)
                           tGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTgcg                                                                                                                           >  2:2330167/1‑150 (MQ=255)
                           tGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTCGATCTTTAATCTGTCGCGTTATCTgcg                                                                                                                           >  2:1550161/1‑150 (MQ=255)
                           tGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTCGATCTTTAATCTGTCGCGTTATCTgcg                                                                                                                           >  2:1550149/1‑150 (MQ=255)
                                  gCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAAc                                                                                                                    >  2:1040718/1‑150 (MQ=255)
                                      aTAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTg                                                                                                                <  2:2312670/150‑1 (MQ=255)
                                        aaGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGaa                                                                                                              <  1:122409/150‑1 (MQ=255)
                                           gCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTa                                                                                                           >  1:256930/1‑150 (MQ=255)
                                           gCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTa                                                                                                           >  1:924239/1‑150 (MQ=255)
                                           gCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTa                                                                                                           >  2:2046577/1‑150 (MQ=255)
                                              gagCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTaaaa                                                                                                        <  2:1438158/150‑1 (MQ=255)
                                                  ttCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACg                                                                                                    >  1:487793/1‑150 (MQ=255)
                                                   tCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGa                                                                                                   <  2:1604413/150‑1 (MQ=255)
                                                   tCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGATATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGa                                                                                                   <  1:668548/150‑1 (MQ=255)
                                                    cgcgCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGAt                                                                                                  >  1:317163/1‑150 (MQ=255)
                                                    cgcgCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGAt                                                                                                  >  1:317175/1‑150 (MQ=255)
                                                    cgcgCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGAt                                                                                                  >  1:317161/1‑150 (MQ=255)
                                                    cgcgCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGAt                                                                                                  <  2:548809/150‑1 (MQ=255)
                                                    cgcgCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGAt                                                                                                  <  1:1094604/150‑1 (MQ=255)
                                                     gcgcCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATg                                                                                                 <  2:89149/150‑1 (MQ=255)
                                                      cgcCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGa                                                                                                <  1:1957569/150‑1 (MQ=255)
                                                      cgcCCAGCAAAGCAAAATAAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGACTGAATCCGGATACCGCTCGCCAGTTGAACTATAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGa                                                                                                <  1:2028162/150‑1 (MQ=255)
                                                       gcCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAg                                                                                               <  2:1460580/150‑1 (MQ=255)
                                                        cccTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGc                                                                                              <  1:1746333/150‑1 (MQ=255)
                                                         ccTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCa                                                                                             <  1:1334567/150‑1 (MQ=255)
                                                           tGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGTACGCTATTTCATCGTCAATCCGTCTGAATCAGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCaat                                                                                           >  2:908148/1‑149 (MQ=255)
                                                           tGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCaaa                                                                                           <  1:2182583/150‑1 (MQ=255)
                                                           tGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCaaa                                                                                           <  1:2182608/150‑1 (MQ=255)
                                                            gcaaagcaaaATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAAt                                                                                          <  2:312916/150‑1 (MQ=255)
                                                             caaagcaaaATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATc                                                                                         <  2:1363132/150‑1 (MQ=255)
                                                             caaagcaaaATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATc                                                                                         <  2:1363144/150‑1 (MQ=255)
                                                              aaagcaaaATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCg                                                                                        <  1:1630738/150‑1 (MQ=255)
                                                                     aaTTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCaa                                                                                 <  2:2359425/150‑1 (MQ=255)
                                                                      aTTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCaaa                                                                                <  1:924601/150‑1 (MQ=255)
                                                                            ccccATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAagag                                                                          <  2:2243878/150‑1 (MQ=255)
                                                                              ccATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCt                                                                        >  2:1604520/1‑150 (MQ=255)
                                                                                aTTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGt                                                                      >  1:2142579/1‑150 (MQ=255)
                                                                                      tGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCaaa                                                                >  1:74999/1‑150 (MQ=255)
                                                                                        tttAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAAtt                                                              >  1:415127/1‑150 (MQ=255)
                                                                                        tttAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGAGACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAAtt                                                              <  1:1859766/150‑1 (MQ=255)
                                                                                        tttAACGCTCTGAACGCTATTTCATCGTCAATCCATCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAAtt                                                              >  2:1239675/1‑150 (MQ=255)
                                                                                           aaCGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTaaa                                                           <  2:915620/150‑1 (MQ=255)
                                                                                            aCGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAg                                                          <  2:681021/150‑1 (MQ=255)
                                                                                             cGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGa                                                         <  2:1478510/150‑1 (MQ=255)
                                                                                              gCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGAc                                                        >  2:2669455/1‑150 (MQ=255)
                                                                                               ctctGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTCGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACt                                                       <  1:1550149/150‑1 (MQ=255)
                                                                                                tctGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACta                                                      <  1:2593220/150‑1 (MQ=255)
                                                                                                tctGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACta                                                      >  2:2001267/1‑150 (MQ=255)
                                                                                                    aaCGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATAtt                                                  >  2:335381/1‑150 (MQ=255)
                                                                                                    aaCGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATAtt                                                  >  2:1717317/1‑150 (MQ=255)
                                                                                                    aaCGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATAtt                                                  <  1:2498229/150‑1 (MQ=255)
                                                                                                       gCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGcc                                               <  1:793637/150‑1 (MQ=255)
                                                                                                       gCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGcc                                               <  1:2460114/150‑1 (MQ=255)
                                                                                                            ttCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGa                                          <  1:841822/150‑1 (MQ=255)
                                                                                                             tCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAg                                         <  2:1780489/150‑1 (MQ=255)
                                                                                                             tCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAg                                         <  2:1754813/150‑1 (MQ=255)
                                                                                                             tCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAg                                         <  2:1754812/150‑1 (MQ=255)
                                                                                                             tCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAg                                         <  1:2232756/150‑1 (MQ=255)
                                                                                                               aTCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAGCa                                       <  2:725314/150‑1 (MQ=255)
                                                                                                               aTCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAGCa                                       >  1:1624688/1‑150 (MQ=255)
                                                                                                               aTCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAGCa                                       <  2:833074/150‑1 (MQ=255)
                                                                                                                tCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAACAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAGCAg                                      <  1:2025885/150‑1 (MQ=255)
                                                                                                                 cGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAGCAgg                                     <  2:1410741/150‑1 (MQ=255)
                                                                                                                 cGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAGCAgg                                     <  1:1456356/150‑1 (MQ=255)
                                                                                                                  gTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAGCAGGc                                    <  1:840500/150‑1 (MQ=255)
                                                                                                                              gAATCCCGATACCGCTCGCCAGTTGATCTTTAATCTGACGCGTTATCTGCGCTATAACATTGAATTAAAACACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATAAAAGACTAAATTGCCATTGAGCAGGCCCGCTTTGgaga                        >  2:495544/1‑150 (MQ=255)
                                                                                                                                aTCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGACTTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAGCAGGCCCGCTTTGGTGACa                      <  1:1917402/150‑1 (MQ=255)
                                                                                                                                 tCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAGCAGGCCCGCTTTGGTGACaa                     >  1:608124/1‑150 (MQ=255)
                                                                                                                                      aTACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGa                <  1:1917072/150‑1 (MQ=255)
                                                                                                                                        aCCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGACg              <  1:876024/150‑1 (MQ=255)
                                                                                                                                        aCCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGACg              <  1:876025/150‑1 (MQ=255)
                                                                                                                                        aCCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGACg              <  1:2473128/150‑1 (MQ=255)
                                                                                                                                        aCCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGACg              <  1:2473126/150‑1 (MQ=255)
                                                                                                                                          cGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGACGGt            >  2:2634627/1‑150 (MQ=255)
                                                                                                                                          cGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGACGGt            >  2:1890580/1‑150 (MQ=255)
                                                                                                                                               gCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGACGGTTATCt       >  2:1088187/1‑150 (MQ=255)
                                                                                                                                                    ttGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGACGGTTATCTATGat  >  2:1956641/1‑150 (MQ=255)
                                                                                                                                                    ttGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGACGGTTATCTATGat  >  2:1956634/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGCCATTGAGCAGGCCCGCTTTGGTGACAAGCTGACGGTTATCTATGAT  >  NC_000913/2499702‑2499999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: