Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,725,832 G→T 8.4% intergenic (‑86/+237) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,725,8320GT 8.4% 524.7 / 7.0 164intergenic (‑86/+237)kgtP/rrfGalpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base G (60/85);  new base T (16/3);  total (76/88)
Fisher's exact test for biased strand distribution p-value = 4.58e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 173 total aligned reads displayed.

AAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGA  >  NC_000913/2725683‑2725981
                                                                                                                                                     |                                                                                                                                                      
aaaTGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAATtttttg                                                                                                                                                        >  2:1933117/1‑150 (MQ=255)
 aaTGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGc                                                                                                                                                       <  1:952613/150‑1 (MQ=255)
       gcgACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCa                                                                                                                                                 <  1:1248638/150‑1 (MQ=255)
         gacgaGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTTTAAAGGAAATATTATACATTTGTTTCATATCAtt                                                                                                                                               >  1:2652756/1‑150 (MQ=255)
            gaGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATg                                                                                                                                            <  1:1894965/150‑1 (MQ=255)
                aTCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGCAATATTATAAATTTTTTTCATATCATTATTCAAc                                                                                                                                        >  2:804772/1‑150 (MQ=255)
                aTCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAAAATTATACATTTGTTGCATATCATTATGAAAc                                                                                                                                        >  2:804715/1‑150 (MQ=255)
                 tCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTTTTAATGAAAAATTATACAATTGTTGCATATAAGTATTCAAcc                                                                                                                                       >  1:1351158/1‑150 (MQ=255)
                     tactTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTaa                                                                                                                                   <  2:2583281/150‑1 (MQ=255)
                        ttGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAAAATTATACATTTGTTTCAAATAAATATGCAAACTTAAACa                                                                                                                                >  2:619455/1‑150 (MQ=255)
                          gTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAATTTGTTGCATATAATTATGCaaccataaccatt                                                                                                                              >  2:1428239/1‑149 (MQ=255)
                             aGTTTGCTGTCTGCCGATACAGTACTAACACCCAAGCCAATATCTCTCCAGCCGTAATCCGATGCAATTGTCGGTCGAATTAGATTATATATTTTGTAAACGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAAt                                                                                                                           <  2:1258897/150‑1 (MQ=255)
                                 tGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATAATATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATttat                                                                                                                       >  2:1727869/1‑149 (MQ=255)
                                 tGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAATTTGTTGCATATCATTATGCAACCTTAACCAAGAATttag                                                                                                                       >  2:1066236/1‑150 (MQ=255)
                                   cTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCAAATCATTATGCAAACATAACCATGAATttagtt                                                                                                                     >  2:2279399/1‑150 (MQ=255)
                                      tCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAAAAATATAAAATTTTTGCATATAAATATTCAACCATAAACATGAAATTAGTTAGc                                                                                                                  >  2:1033763/1‑150 (MQ=255)
                                      tCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTGTTAAGGGAATATTATTAATTTGTTGCCTATCATTTTGCCACCGTAAACATGAATGTTGTTTGc                                                                                                                  >  1:79485/1‑150 (MQ=255)
                                       cTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCa                                                                                                                 <  1:278179/150‑1 (MQ=255)
                                           cGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGGTTTTTTATTTTTTTTTTTAATGAAAAATTATAAAATTTTTTGATATAAATATTGAAACATAAAAAAGAAATTAGTTAGAAGGaa                                                                                                            >  1:729468/1‑150 (MQ=255)
                                              tACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAAt                                                                                                         <  1:2462023/150‑1 (MQ=255)
                                              tACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAAt                                                                                                         <  1:2412274/150‑1 (MQ=255)
                                               aCAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATg                                                                                                        <  1:618309/150‑1 (MQ=255)
                                               aCAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATg                                                                                                        <  1:926801/150‑1 (MQ=255)
                                                      tttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTTTTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTATCAGGAAAATGGTTATTg                                                                                                 <  1:1025429/150‑1 (MQ=255)
                                                      tttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTg                                                                                                 <  1:1025301/150‑1 (MQ=255)
                                                       ttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTga                                                                                                <  2:1775793/150‑1 (MQ=255)
                                                          aGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTgagga                                                                                             <  2:2660548/150‑1 (MQ=255)
                                                           gccatgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTgaggag                                                                                            <  2:2133576/150‑1 (MQ=255)
                                                           gccatgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGGTTGTTTTTTTTTTTTTTTAATGGAAAAATATAAAATTTTTTGATATCAATTTGAAAACAAAAACAATAAATTTGTTATCAGGAAAATTGTttttttgggg                                                                                            >  1:2134539/1‑144 (MQ=255)
                                                                gccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTaa                                                                                       <  2:1481325/150‑1 (MQ=255)
                                                                 ccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTTTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAg                                                                                      >  2:2681133/1‑150 (MQ=255)
                                                                 ccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAg                                                                                      <  2:409185/150‑1 (MQ=255)
                                                                  catTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAAACATAACCATGAATTTATTTATCAGGAAAATGGTTATTTAGGAGCTTAAgg                                                                                     >  2:1936093/1‑150 (MQ=255)
                                                                   atTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGa                                                                                    <  1:1825701/150‑1 (MQ=255)
                                                                    ttATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGAt                                                                                   >  2:697202/1‑150 (MQ=255)
                                                                     tATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATa                                                                                  <  2:851802/150‑1 (MQ=255)
                                                                         tctcCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGTTAAAtt                                                                              >  2:1070339/1‑150 (MQ=255)
                                                                           tcCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTc                                                                            <  1:735025/150‑1 (MQ=255)
                                                                           tcCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTc                                                                            <  2:1065640/150‑1 (MQ=255)
                                                                             cTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTg                                                                          <  1:1960059/150‑1 (MQ=255)
                                                                             cTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTg                                                                          <  1:2230800/150‑1 (MQ=255)
                                                                             cTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTg                                                                          <  1:2733351/150‑1 (MQ=255)
                                                                                 cGTAATCCGTTGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAATGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAATTGGTTTTTGAGGAGCTTAAGGATAAATTTCTGGTaa                                                                      <  1:2300873/150‑1 (MQ=255)
                                                                                 cGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTaa                                                                      <  2:812106/150‑1 (MQ=255)
                                                                                  gTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAag                                                                     <  2:1651197/150‑1 (MQ=255)
                                                                                    aaTCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAagga                                                                   <  1:149205/150‑1 (MQ=255)
                                                                                    aaTCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAagga                                                                   <  1:2257127/150‑1 (MQ=255)
                                                                                      tCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAATTTTTTGCATATCATTATGCAAACTTAAACAAGAATTTAGTTAGCAGGAAAATTGGTATTTTGGAGCTTAATGATAAATTTGTGGTAaggagg                                                                 >  2:58707/1‑150 (MQ=255)
                                                                                        cGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTTAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGac                                                               >  2:2735392/1‑150 (MQ=255)
                                                                                            gCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGt                                                           <  1:1471082/150‑1 (MQ=255)
                                                                                              ttttGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTAt                                                         <  2:1957880/150‑1 (MQ=255)
                                                                                                ttGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAATGCTAAATTTATGGTAAGGAGGACACGTATgg                                                       >  2:1613132/1‑150 (MQ=255)
                                                                                                  gtcggtcgCTTTTGTTTATTTTTTTTGTAAAGTAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTGAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGaa                                                     <  2:2033646/150‑1 (MQ=255)
                                                                                                  gtcggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGaa                                                     <  2:132473/150‑1 (MQ=255)
                                                                                                  gtcggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGaa                                                     <  2:1351158/150‑1 (MQ=255)
                                                                                                  gtcggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGaa                                                     <  2:1511622/150‑1 (MQ=255)
                                                                                                  gtcggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCATAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGaa                                                     <  2:885636/150‑1 (MQ=255)
                                                                                                    cggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGt                                                   <  1:91365/150‑1 (MQ=255)
                                                                                                    cggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGt                                                   <  1:1993842/150‑1 (MQ=255)
                                                                                                    cggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGt                                                   <  1:1050997/150‑1 (MQ=255)
                                                                                                    cggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTACGGAAGt                                                   <  1:1051198/150‑1 (MQ=255)
                                                                                                    cggtcgCTTTTGTTAACTTTTTTTGAACAGGAAATATTATACATTTGTTGCATCTCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGt                                                   <  2:1670022/150‑1 (MQ=255)
                                                                                                     ggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTg                                                  <  1:259386/150‑1 (MQ=255)
                                                                                                     ggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTg                                                  <  1:1610161/150‑1 (MQ=255)
                                                                                                      gtcgCTTTTGTTTATTTTTTTTTTAAAGGAAAAATTATAAAATTTTTGCATATAAATATGGAACCATAACCATTAAATTAGTTATCAGGAAAATTTGTATTTAGGAGCTTAAGGTTAAATTTATGGTAAGGAGGACAAGGATGGTAGttg                                                 >  2:523506/1‑148 (MQ=255)
                                                                                                      gtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTgg                                                 <  1:485512/150‑1 (MQ=255)
                                                                                                      gtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTgg                                                 <  2:412335/150‑1 (MQ=255)
                                                                                                      gtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTgg                                                 <  1:1780029/150‑1 (MQ=255)
                                                                                                           ttttGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCTAg                                            >  1:326382/1‑150 (MQ=255)
                                                                                                               gTTTATTTTTTTTTTAAAGGAAATATTATACATTTGTTGCAAATCATTATGAAACATAAACCAAGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTATGGTAAGGAGGACACGTATGGAAGTGGGCACGTTgg                                        >  2:2222704/1‑150 (MQ=255)
                                                                                                                   aTTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGaa                                    >  1:1372636/1‑150 (MQ=255)
                                                                                                                   aTTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGaa                                    <  2:2524628/150‑1 (MQ=255)
                                                                                                                    ttttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACAATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGCGCTTAAGGATAAATTTCTGGTAAGGAGGACAAGTATGGAAGTGGGCAAGTTGGGGAAg                                   >  1:313359/1‑150 (MQ=255)
                                                                                                                     tttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGc                                  <  2:2130463/150‑1 (MQ=255)
                                                                                                                     atattttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGc                                  <  1:1640577/147‑1 (MQ=255)
                                                                                                                       tttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCg                                >  1:1154674/1‑150 (MQ=255)
                                                                                                                       tttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCg                                >  1:1154319/1‑150 (MQ=255)
                                                                                                                        ttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTCAGGATAAAATTCTGGTAAGGATGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGt                               >  2:1636462/1‑150 (MQ=255)
                                                                                                                        ttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTGATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGt                               >  1:180489/1‑150 (MQ=255)
                                                                                                                            gTAAATGTAATTATATTCATTTTTTTCATTTCATTATTCAAACTTAACCATGTATTTAGTTAGCATGTAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATc                            <  1:619455/150‑1 (MQ=255)
                                                                                                                            gTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATcc                           <  2:889966/150‑1 (MQ=255)
                                                                                                                            gTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATcc                           <  1:270831/150‑1 (MQ=255)
                                                                                                                            gTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATcc                           <  2:1126765/150‑1 (MQ=255)
                                                                                                                             tAAAGGAAGTATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAATATGGTTATTGAGGAGGTTAAGGATATATTTGTGGTGAGGTGGAGACGTTTGGTAGTGGGGAGGTTGGGGTAGCCGTGTCCg                          >  1:2361011/1‑150 (MQ=255)
                                                                                                                               aaGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGtt                        >  2:1569199/1‑150 (MQ=255)
                                                                                                                                aGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTg                       <  2:214902/150‑1 (MQ=255)
                                                                                                                                 ggAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGc                      >  2:310984/1‑150 (MQ=255)
                                                                                                                                 ggAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGc                      >  1:2548392/1‑150 (MQ=255)
                                                                                                                                 ggAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGc                      >  2:662737/1‑150 (MQ=255)
                                                                                                                                  gAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCt                     <  2:713425/150‑1 (MQ=255)
                                                                                                                                   aaaTATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGGTGGGGACGCCGTATCCGTTGCTg                    >  2:4262/1‑150 (MQ=255)
                                                                                                                                     atatTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGaa                  >  1:1925522/1‑150 (MQ=255)
                                                                                                                                         tataCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTg              >  1:66295/1‑150 (MQ=255)
                                                                                                                                         tataCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTg              >  1:725147/1‑150 (MQ=255)
                                                                                                                                          ataCATTTGGTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTgg             <  2:1556965/150‑1 (MQ=255)
                                                                                                                                                   ttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTgg    <  2:110661/150‑1 (MQ=255)
                                                                                                                                                   ttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTgg    >  2:2748426/1‑150 (MQ=255)
                                                                                                                                                     gCATATCCTTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGa  <  1:367688/150‑1 (MQ=255)
                                                                                                                                                     gCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGa  <  2:555536/150‑1 (MQ=255)
                                                                                                                                                     gCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGa  <  2:2320064/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
AAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGA  >  NC_000913/2725683‑2725981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: