BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,817,916	T→G	5.8%	intergenic (‑56/+143)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,817,916	0	T	G	5.8%	425.6 / 15.4	138	intergenic (‑56/+143)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base T (54/76); new base G (6/2); total (60/78)
Fisher's exact test for biased strand distribution p-value = 7.78e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 143 total aligned reads displayed.

GAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCA  >  NC_000913/2817767‑2818065

 aaGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAtt                                                                                                                                                      <  1:218530/150‑1 (MQ=255)

 aaGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAtt                                                                                                                                                      <  1:218484/150‑1 (MQ=255)

    gTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAag                                                                                                                                                   <  1:1509525/150‑1 (MQ=255)

    gTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAag                                                                                                                                                   <  1:1509487/150‑1 (MQ=255)

      tACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAagag                                                                                                                                                 >  1:258172/1‑150 (MQ=255)

          gagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGG                                                                                                                                             >  1:651687‑M1/1‑150 (MQ=255)

           agaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGT                                                                                                                                            <  1:2070033‑M1/150‑1 (MQ=255)

                 tGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCC                                                                                                                                      <  2:2299604‑M1/150‑1 (MQ=255)

                   gTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGTAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGG                                                                                                                                    <  2:230172‑M1/150‑1 (MQ=255)

                   gTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGG                                                                                                                                    <  1:584648‑M1/150‑1 (MQ=255)

                   gTGCAACCGGGAGGATTCGAACCTCCGACCGCTCGGATCGTAGCCGAGTACTCAATCCAGCTGACCTACGGATGCATCGGGAAAACATATACTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGG                                                                                                                                    <  2:2072804‑M1/150‑1 (MQ=255)

                     tcATCCGGGAGGATTCGAACCTCCCAACGCTCGGTTCCTAGCCGATTACTCTATCCAGCTGAGCTACGGATGCATCTGGAAAACTTATACTACTGCTGATATTGATACCGCTACTAAAGCAATATCAATTAATAGATGGTGCATCCGgga                                                                                                                                  <  1:1348037/149‑1 (MQ=14)

                     gCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGA                                                                                                                                  <  2:2062949‑M1/150‑1 (MQ=255)

                     gCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGA                                                                                                                                  <  1:840599‑M1/150‑1 (MQ=255)

                     gCATCCGGGAGGATTCGAACCACCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCACCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTCCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGA                                                                                                                                  <  2:292047‑M1/150‑1 (MQ=255)

                     gCATCCGGGAGGAATCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACTGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTAATAAAGCAATATCAATTAAGAGATGGTGCATCCGGGA                                                                                                                                  <  1:1348201‑M1/150‑1 (MQ=255)

                          cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATT                                                                                                                             <  1:394237‑M1/150‑1 (MQ=255)

                          cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTATACTGCTGATATTGATACCGCTACTAACGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATT                                                                                                                             <  2:905259‑M1/150‑1 (MQ=255)

                             gaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACt                                                                                                                          <  1:2237557/150‑1 (MQ=255)

                                  ttCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCt                                                                                                                     <  1:1468672/150‑1 (MQ=255)

                                  ttCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCt                                                                                                                     >  2:2357330/1‑150 (MQ=255)

                                     gAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGc                                                                                                                  >  1:599678/1‑150 (MQ=255)

                                      aaCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCg                                                                                                                 >  1:1472337/1‑150 (MQ=255)

                                      aaCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCg                                                                                                                 <  2:2569608/150‑1 (MQ=255)

                                        ccTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATTCATCGTGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGc                                                                                                               <  2:2194675/150‑1 (MQ=255)

                                         cTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCt                                                                                                              <  2:1446485/150‑1 (MQ=255)

                                          tCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTc                                                                                                             <  1:1115607/150‑1 (MQ=255)

                                           ccGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCg                                                                                                            <  2:2115638/150‑1 (MQ=255)

                                           acGACCGCTCTGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACTGATGCATCGGTAAAACTTATTCTACTGCTTATATTGATACCGCTTCTAAATCCATATCTATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCg                                                                                                            <  2:1631534/149‑1 (MQ=17)

                                                 gCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTc                                                                                                      <  2:342207/150‑1 (MQ=255)

                                                   tCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTAAGTCTACTGCTGATAGTGATCCCGCTTCTAAAGCCATATCAATTAAGAGCGGGTGCGTCCGGGAGGATTACTCGCCTTGCGGCTCGcccggcgg                                                                                                    >  1:1057941/1‑145 (MQ=17)

                                                        tCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGt                                                                                               <  1:290972/150‑1 (MQ=255)

                                                         cGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGtt                                                                                              <  2:657325/150‑1 (MQ=255)

                                                              ccGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTaa                                                                                         >  1:1763391/1‑150 (MQ=255)

                                                              ccGAGGACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCCTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCttt                                                                                         <  1:798346/150‑3 (MQ=255)

                                                               cgtgTACTCTATCCCGCTGAGCTACGGATGCATGTGGAAAACTTATTATACTGCTGCTATTGATACCGCTTCTAAAGCCATTTCAATTATGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTaaa                                                                                        <  2:1508922/147‑1 (MQ=17)

                                                               cGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTaaa                                                                                        <  1:2660344/150‑1 (MQ=255)

                                                                    aCTCTATCCAGCTGAGCTACGGTTGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAAc                                                                                   <  1:2693790/150‑1 (MQ=255)

                                                                       ctATCCAGCTGAGCTACGGATGCATCGGGAAAAATTATTCTACTGATGATATTGAAACCGCAACTAAACCCATATAAATTAAGAGATGGTGCAGCCGGGAGGATTACTCGCCTTGCGGCTAGCCCTACGGGCCGTAGCTAAAGAAAcata                                                                                >  2:517188/1‑147 (MQ=17)

                                                                        tATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTa                                                                               <  1:812643/150‑1 (MQ=255)

                                                                          tCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatc                                                                             >  1:410474/1‑150 (MQ=255)

                                                                          tCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatc                                                                             >  2:1117907/1‑150 (MQ=255)

                                                                            cAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatcat                                                                           >  2:2348323/1‑150 (MQ=255)

                                                                            cAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatcat                                                                           <  1:199673/150‑1 (MQ=255)

                                                                              gCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATcc                                                                         <  1:113838/150‑1 (MQ=255)

                                                                              gCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATcc                                                                         >  1:697528/1‑150 (MQ=255)

                                                                              gCTGAGCTACGGATGCATCGGGAAAACTTATTCGACTGCTGATATTGATACCGCTACTACAGCCATATCAATTATGAGATGGTGCATCCGGGTGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATcc                                                                         <  1:1562093/150‑1 (MQ=255)

                                                                               cTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATccc                                                                        <  1:2130998/150‑1 (MQ=255)

                                                                               cTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATccc                                                                        <  1:100343/150‑1 (MQ=255)

                                                                                   gCTACGGATGCATCGGGCACACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCATTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGt                                                                    <  1:2386790/150‑1 (MQ=255)

                                                                                   gCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGt                                                                    <  2:410466/150‑1 (MQ=255)

                                                                                   gCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGt                                                                    <  2:1167819/150‑1 (MQ=255)

                                                                                     tACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGc                                                                  >  1:1868414/1‑150 (MQ=255)

                                                                                      aCGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTTAAGCCATATCATTTATGAGATGGTGCATCCGGGAGGATTACTCGCATTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGcc                                                                 <  2:1240854/150‑1 (MQ=255)

                                                                                       cGGATGCATCGTGAAAACTTATTCTACTGCTGATATTTATCCCGCTACTATAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCt                                                                <  1:1419637/150‑1 (MQ=255)

                                                                                        ggATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTg                                                               >  1:2428855/1‑150 (MQ=255)

                                                                                         gATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGc                                                              >  2:2191326/1‑150 (MQ=255)

                                                                                         gATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGc                                                              >  2:627133/1‑150 (MQ=255)

                                                                                         gATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACACGCCAAGAGCCAACCCATACTGGCCGATTCAAAAGAAACGATGTCATCCCTAGtcacagc                                                              >  1:1005581/1‑144 (MQ=14)

                                                                                          aTGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCg                                                             >  2:23261/1‑150 (MQ=255)

                                                                                          aTGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAAACGTGCTACATCCGGGAGGGGAACTAGCCTTGCGTATCGCGCTTCGCGCCGTTGAGCACGCAACGTTAACATCCCTAGtgcgtgcg                                                             >  2:1395249/1‑150 (MQ=18)

                                                                                          aTGCATCGGGAAAACTGATTCCACGGCTGATATTGATACCGATAATAAAGCGATATCTATTATGCGATGGTGCCTCCGGGAGGATTTGTCGCCGTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCg                                                             <  2:1982239/150‑1 (MQ=18)

                                                                                          aTGCATCGGGAAAACGTAGTCTACGGCTGAGATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCg                                                             <  2:1982073/150‑1 (MQ=255)

                                                                                           tGCATCGGGAAAACTTATTCTACTGCCGATATTGATACCGCTACGAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCgg                                                            <  2:2086085/150‑1 (MQ=255)

                                                                                              aTCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTAGCGGCTCGCCCTGCGGGCCGTTGCTAAAGCACCGTTATCATCCCTAGTGCATGCGGTTa                                                         >  2:2088418/1‑150 (MQ=255)

                                                                                               tCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTaa                                                        >  1:2252042/1‑150 (MQ=255)

                                                                                               tCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTaa                                                        >  1:375835/1‑150 (MQ=255)

                                                                                                  ggAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTc                                                     >  1:310616/1‑150 (MQ=255)

                                                                                                     aaaCTTATTCTTCTTCTTATATTGAGACCGCTACTTAAGCCATATCAATTTTGAGATTGTGCTTCCGGGTGGTTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGc                                                  <  1:1819954/150‑1 (MQ=17)

                                                                                                      aaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCt                                                 <  2:2615411/150‑1 (MQ=255)

                                                                                                       aCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTa                                                >  2:2446410/1‑150 (MQ=255)

                                                                                                         ttATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTACAGCACCGATATCATCCCTAGGGCCTGCGGTTAATACCGCTAcc                                              >  2:2574434/1‑150 (MQ=17)

                                                                                                         ttATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagct                                                                                >  2:505575‑M1/1‑71 (MQ=255)

                                                                                                             tCTACTTCTTATATTGATACAGCTACTACAGCCATATCTATTTCGAGATGGTGCCTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCtt                                          <  1:2615986/150‑1 (MQ=17)

                                                                                                             tCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGGATACGCGCATGGCCGCTCGCGCTACGGGCCGGTGCTAGGGCCACGATATCGTCGCTAGTGCGAGCGGTTAATTCCGCTAGctctg                                          >  2:2397110/1‑149 (MQ=17)

                                                                                                             tCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCtt                                          >  1:986331/1‑150 (MQ=255)

                                                                                                              cTACTGATTTTATTGAGACCTCTAATAAAGCCATATCTATTAATAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTc                                         <  1:895325/150‑1 (MQ=17)

                                                                                                                 ctgctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGAt                                      >  1:2031674/1‑150 (MQ=255)

                                                                                                                  tgctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCAGGGAGTATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGATATCATCCCTAGTGCCTGCGTTTAATACCGCTACCTCTACGATa                                     >  1:72262/1‑150 (MQ=17)

                                                                                                                  tgctgATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaactggcggggaggcgggcattcgaaccca                                     >  2:1293730‑M1/1‑62 (MQ=255)

                                                                                                                   gctgATATTGATACCGCTACTAAAGCCATATCAATTACGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATAc                                    <  1:1802642/150‑1 (MQ=255)

                                                                                                                     tgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCt                                  <  2:2701218/150‑1 (MQ=255)

                                                                                                                     tgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCCCCGAGCGCCTCGCCCTACGGGCCGATGCGAAAGCACCGATATCATCCCTAGTCCCTGCGGTTAATACCGCTAGCGATTAGATACCt                                  >  2:2667876/1‑150 (MQ=14)

                                                                                                                       atatTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCATGAGGCGCGCCCTACGGGCCGTTGCTAAAGAAACGATATCATCCCTAGTGCCAGCGGTTAATTCCGCTACCTCTTCGATACCTTc                                >  1:455145/1‑150 (MQ=17)

                                                                                                                       atatTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaaccccggatg                                >  2:493469‑M1/1‑57 (MQ=255)

                                                                                                                          ttGATACCGCTACTAAAGCCATATCTATTAAGTTCTTGAGCATCCGGGAGGATTCCTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCAtt                             <  1:141715/150‑1 (MQ=17)

                                                                                                                          ttGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCAtt                             <  1:141822/150‑1 (MQ=255)

                                                                                                                           tGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTg                            >  2:1768335/1‑150 (MQ=255)

                                                                                                                           tGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTg                            >  2:83191/1‑150 (MQ=255)

                                                                                                                              tACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTg                         <  2:651687/150‑1 (MQ=255)

                                                                                                                              tACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTAGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGATg                         >  1:2328280/1‑150 (MQ=255)

                                                                                                                               aCCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTAGATACCTTCATTGCTGa                        <  1:1622650/150‑1 (MQ=255)

                                                                                                                                   ctactaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAt                    >  2:1028461/1‑150 (MQ=255)

                                                                                                                                    tactaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACACGCAAAGAGCCGCGCCCTACTGGCCGTTTCAAAAGAAACGATCACATCCCTAGTGCCTGCGGATAATTCCGCAACCTCTTAGATACCTTCATTGCTGAAGaat                   >  2:2244549/1‑148 (MQ=17)

                                                                                                                                     actaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACACGCAAAGACCCTCGCCATGCGGGCCGTTGAGAAAGCTAACTTAACATCCCTCGTGACTGCGGTTAATTCCGCTACCTATTCGATAACTTCATTCCTGAAGAatt                  >  2:300602/1‑150 (MQ=12)

                                                                                                                                      ctaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTc                 >  2:1338204/1‑150 (MQ=255)

                                                                                                                                       taAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCATAGTGACTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCg                <  2:1894142/150‑1 (MQ=255)

                                                                                                                                         aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga              <  1:2357330/150‑1 (MQ=255)

                                                                                                                                            ccATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaaccccggatgcagcttttgaccgcatactcc           >  2:220680‑M1/1‑36 (MQ=255)

                                                                                                                                                atCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATg       <  2:668581/150‑1 (MQ=255)

GAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCA  >  NC_000913/2817767‑2818065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`