Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,215,694 G→T 5.2% intergenic (+21/+33) ileX → / ← yqjH tRNA‑Ile/putative siderophore interacting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,215,6940GT 5.2% 576.8 / 12.6 161intergenic (+21/+33)ileX/yqjHtRNA‑Ile/putative siderophore interacting protein
Reads supporting (aligned to +/- strand):  ref base G (77/74);  new base T (4/6);  total (81/80)
Fisher's exact test for biased strand distribution p-value = 5.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 164 total aligned reads displayed.

GCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAG  >  NC_000913/3215548‑3215841
                                                                                                                                                  |                                                                                                                                                   
gcgGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGaaa                                                                                                                                                  >  2:1477439/1‑150 (MQ=255)
  ggTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATc                                                                                                                                                <  2:2235166/150‑1 (MQ=255)
    tAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGt                                                                                                                                              <  1:1033482/150‑1 (MQ=255)
    tAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGt                                                                                                                                              <  1:2221554/150‑1 (MQ=255)
    tAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGt                                                                                                                                              <  2:2732697/150‑1 (MQ=255)
     aaTGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTc                                                                                                                                             <  1:1725475/150‑1 (MQ=255)
        ggCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGATTTCTCGTCAGc                                                                                                                                          <  2:1303762/150‑1 (MQ=255)
        ggCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGc                                                                                                                                          <  2:2509641/150‑1 (MQ=255)
         gCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGcc                                                                                                                                         <  1:2493886/150‑1 (MQ=255)
         gCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGcc                                                                                                                                         <  1:1778864/150‑1 (MQ=255)
         gCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCGTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGAGATAGCAAAGGCTGACGAGAAATCGTCAGcc                                                                                                                                         >  2:2395103/1‑150 (MQ=255)
          cTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCct                                                                                                                                        >  1:1697367/1‑150 (MQ=255)
           tGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctt                                                                                                                                       >  1:718120/1‑150 (MQ=255)
            ggATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGATTTCTCGTCAGCcttt                                                                                                                                      <  2:1144883/150‑1 (MQ=255)
             gATTGCGACACGGAGGGACTTTATACTCCGCTACCATGGCCCCGTCGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGAGAGAGCAAAGGCTGACGATTTCTCGTCAGCctttg                                                                                                                                     <  2:813056/150‑2 (MQ=21)
               ttGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttc                                                                                                                                   >  2:1404015/1‑150 (MQ=255)
                  cGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAATGGCTGACGAGAAATCGTCAGCctttttcttt                                                                                                                                <  1:959150/150‑1 (MQ=255)
                   gACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttctttt                                                                                                                               >  1:2428349/1‑150 (MQ=255)
                   gACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttctttt                                                                                                                               <  1:1616338/150‑1 (MQ=255)
                   gACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctgtttctttt                                                                                                                               >  2:1185212/1‑150 (MQ=255)
                    acacGGAGTTACTTTATAATCCGCTACCATGGCCCCGTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttcttttt                                                                                                                              <  2:810137/150‑1 (MQ=255)
                      acGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTtat                                                                                                                            <  2:200094/150‑1 (MQ=255)
                      acGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTtat                                                                                                                            >  1:1334490/1‑150 (MQ=255)
                      acGGAGTTACTTTATAATCCGCTACCATGGCCCCTGAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTtat                                                                                                                            <  2:1830491/150‑1 (MQ=255)
                        ggAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTtatat                                                                                                                          >  2:949851/1‑150 (MQ=255)
                            ttACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCa                                                                                                                      <  2:2581598/150‑1 (MQ=255)
                              aCTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGt                                                                                                                    >  1:85913/1‑150 (MQ=255)
                              aCTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGt                                                                                                                    >  1:85669/1‑150 (MQ=255)
                              aCTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGt                                                                                                                    <  1:645224/150‑1 (MQ=255)
                              aCTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGt                                                                                                                    <  1:2615903/150‑1 (MQ=255)
                                tttATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTa                                                                                                                  >  1:823534/1‑150 (MQ=255)
                                tttATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTa                                                                                                                  >  2:1345847/1‑150 (MQ=255)
                                tttATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTa                                                                                                                  >  1:2727861/1‑150 (MQ=255)
                                tttATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTa                                                                                                                  >  2:2111098/1‑150 (MQ=255)
                                     aaTCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTg                                                                                                             >  1:197652/1‑150 (MQ=255)
                                       tCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCg                                                                                                           >  1:1571412/1‑150 (MQ=255)
                                       tCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTGATATCGCAGTTACTTTGCg                                                                                                           >  2:315581/1‑150 (MQ=255)
                                        ccGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGt                                                                                                          >  2:1807769/1‑150 (MQ=255)
                                        ccGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGt                                                                                                          <  1:764182/150‑1 (MQ=255)
                                           cTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGcc                                                                                                       >  2:1963449/1‑150 (MQ=255)
                                            tACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCa                                                                                                      <  2:2353726/150‑1 (MQ=255)
                                            tACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCa                                                                                                      <  2:705443/150‑1 (MQ=255)
                                             aCCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAg                                                                                                     >  2:1569862/1‑150 (MQ=255)
                                                  ggCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGc                                                                                                <  1:389886/150‑1 (MQ=255)
                                                          aGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCAc                                                                                        <  2:2235734/150‑1 (MQ=255)
                                                             tCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTa                                                                                     >  1:2108208/1‑150 (MQ=255)
                                                                gTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTAccc                                                                                  >  2:2029985/1‑150 (MQ=255)
                                                                gTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTAccc                                                                                  >  1:2146128/1‑150 (MQ=255)
                                                                      agagCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCtgtg                                                                            >  1:2052082/1‑150 (MQ=255)
                                                                       gagCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTgg                                                                           <  1:2209231/150‑1 (MQ=255)
                                                                       gagCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTgg                                                                           >  1:1234916/1‑150 (MQ=255)
                                                                       gagCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTgg                                                                           <  2:2368608/150‑1 (MQ=255)
                                                                       gagCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTgg                                                                           >  2:2183763/1‑150 (MQ=255)
                                                                          cAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTc                                                                        >  2:2653586/1‑150 (MQ=255)
                                                                          cAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTc                                                                        >  2:236616/1‑150 (MQ=255)
                                                                           aGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCa                                                                       >  1:102898/1‑150 (MQ=255)
                                                                                aCTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTg                                                                  >  1:588968/1‑150 (MQ=255)
                                                                                    aTAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCATTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTcc                                                              <  2:584909/150‑1 (MQ=255)
                                                                                      aaTCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGc                                                            >  1:2113225/1‑150 (MQ=255)
                                                                                       aTCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCt                                                           <  1:968681/150‑1 (MQ=255)
                                                                                       aTCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGAGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCt                                                           >  1:2644047/1‑150 (MQ=255)
                                                                                         cGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTc                                                         <  1:1404015/150‑1 (MQ=255)
                                                                                            ttGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCGCGTACCCGCTGTGGGTCATACTGTTCCGCTTCaaa                                                      >  1:1432847/1‑150 (MQ=255)
                                                                                            ttGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCaaa                                                      >  1:911667/1‑150 (MQ=255)
                                                                                              ggTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAgc                                                    <  2:689330/150‑1 (MQ=255)
                                                                                                tCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAgcgg                                                  >  2:2659385/1‑150 (MQ=255)
                                                                                                  gCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAgcggcg                                                >  1:612917/1‑150 (MQ=255)
                                                                                                    tGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCggcggc                                              <  2:2564701/150‑1 (MQ=255)
                                                                                                    tGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCggcggc                                              >  1:666616/1‑150 (MQ=255)
                                                                                                    tGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCggcggc                                              >  1:2091783/1‑150 (MQ=255)
                                                                                                       ttCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCtaa                                           >  1:2713085/1‑148 (MQ=255)
                                                                                                        tCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAATCGGCGGCTTaa                                          >  1:2523409/1‑150 (MQ=255)
                                                                                                        tCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTaa                                          <  1:2600160/150‑1 (MQ=255)
                                                                                                        tCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTaa                                          >  2:720957/1‑150 (MQ=255)
                                                                                                             tCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCTGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCt                                     >  2:1886859/1‑150 (MQ=255)
                                                                                                             tCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCt                                     >  2:1880896/1‑150 (MQ=255)
                                                                                                               cagcagGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTa                                   <  2:838897/150‑1 (MQ=255)
                                                                                                                agcagGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTaa                                  >  1:1330234/1‑150 (MQ=255)
                                                                                                                     gggCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACt                             >  1:1785323/1‑150 (MQ=255)
                                                                                                                        ccaccaGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCGTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTa                          <  2:1038616/150‑1 (MQ=255)
                                                                                                                          accaGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTAcc                        >  1:80405/1‑150 (MQ=255)
                                                                                                                          accaGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTAcc                        >  1:2385935/1‑150 (MQ=255)
                                                                                                                          accaGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTAcc                        >  2:1814195/1‑150 (MQ=255)
                                                                                                                             aGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTc                     >  1:541134/1‑150 (MQ=255)
                                                                                                                              tatatAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTTTATATCTGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAACGCGGCGGATTAAATTCTTAACGACTTTACCTTCg                    <  1:1353829/149‑1 (MQ=255)
                                                                                                                                 ataGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCg                 <  2:588968/150‑1 (MQ=255)
                                                                                                                                 ataGCAAAGGCTGACGAGAAATCGTCAGCATTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCg                 <  1:1497266/150‑1 (MQ=255)
                                                                                                                                      aaaGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTAt            >  1:1501186/1‑150 (MQ=255)
                                                                                                                                      aaaGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTAt            >  1:1138227/1‑150 (MQ=255)
                                                                                                                                      aaaGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTAt            >  1:1137850/1‑150 (MQ=255)
                                                                                                                                       aaGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATc           <  2:453062/150‑1 (MQ=255)
                                                                                                                                           cTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGa       >  1:1547024/1‑150 (MQ=255)
                                                                                                                                            tGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCACCGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGAt      <  1:236616/150‑1 (MQ=255)
                                                                                                                                            tGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGAt      <  1:2653586/150‑1 (MQ=255)
                                                                                                                                            tGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTGGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGAt      <  1:2430734/150‑1 (MQ=255)
                                                                                                                                              aCGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGa    <  2:2385710/150‑1 (MQ=255)
                                                                                                                                              aCGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGa    <  2:2385697/150‑1 (MQ=255)
                                                                                                                                              aCGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGa    <  2:2123654/150‑1 (MQ=255)
                                                                                                                                              aCGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGa    <  2:2123651/150‑1 (MQ=255)
                                                                                                                                               cGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGaa   <  1:1569862/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAG  >  NC_000913/3215548‑3215841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: