Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,233,708 A→G 5.1% intergenic (+25/+20) fadH → / ← higA 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked/antitoxinof the HigB‑HigA toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,233,7080AG 5.1% 389.2 / 7.7 117intergenic (+25/+20)fadH/higA2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked/antitoxinof the HigB‑HigA toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base A (62/49);  new base G (3/3);  total (65/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01

Only 100 of 122 total aligned reads displayed.

CGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCG  >  NC_000913/3233559‑3233857
                                                                                                                                                      |                                                                                                                                                     
cgcTGGCGCAATCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCa                                                                                                                                                       >  1:202290/1‑150 (MQ=255)
cgcTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCa                                                                                                                                                       <  1:1276467/150‑1 (MQ=255)
 gcTGGCGCAACCGCTGATTGATTGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCaa                                                                                                                                                      <  1:850895/150‑1 (MQ=255)
  cgggCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAAc                                                                                                                                                     <  1:1197313/148‑1 (MQ=255)
  cTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAAc                                                                                                                                                     <  1:2376846/150‑1 (MQ=255)
     gcgcAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCa                                                                                                                                                  >  2:669848/1‑150 (MQ=255)
     gcgcAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCa                                                                                                                                                  >  1:293309/1‑150 (MQ=255)
     gcgcAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCa                                                                                                                                                  >  1:195095/1‑150 (MQ=255)
     gcgcAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCa                                                                                                                                                  >  1:1343276/1‑150 (MQ=255)
        cAACCGCTGTTTGATAGCGGGAAAACGGTGCATTTAATGGGCGGCTGCGATGTGGCTATGGAGCTGGACGCTCGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATcc                                                                                                                                               <  2:100407/150‑1 (MQ=255)
        cAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAGCACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATcc                                                                                                                                               <  2:100404/150‑1 (MQ=255)
        cAACCGCTGATTGATAGCGGGAAAACGGAGCAATTAAATGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATcc                                                                                                                                               <  2:460683/150‑1 (MQ=255)
          aCCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCGTTGCATCCgg                                                                                                                                             <  2:511400/150‑1 (MQ=255)
             gCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCGTTGCATCCGGcgc                                                                                                                                          <  2:1732823/150‑3 (MQ=255)
              cTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACg                                                                                                                                         >  2:1903754/1‑150 (MQ=255)
               tgattgatAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGt                                                                                                                                        >  1:2341635/1‑150 (MQ=255)
                   tgatAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGGcgcg                                                                                                                                    >  1:1335556/1‑150 (MQ=255)
                       cgCGGGAACACGGTGCCTTCACGTGGCGGCTGGGATGGGGCAATGGAGCTGGCCGCCCGACGGGCACTGGCCCAGGGAACCCGGCTGGGGCGGGAGATTTAAATCGTTATTTGACGCGCCGGATGCACCGCTTCCGGCACGTCGCGTTaa                                                                                                                                <  2:1847884/149‑1 (MQ=255)
                       aGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTaa                                                                                                                                >  1:1955835/1‑150 (MQ=255)
                         ggggTAAAAGGTGGATTTTATTTGGCGGCTGCGTTGGGGCTTTGGCGCTGGACGCACGCGGGGAATTTGCCCTGGGAACCGGGGTGGGGCTGTAGATTTAAATGGTTTTTTGACGCGCCGGTTGCAAGGCATCCGGCACGTCGCGTTAAt                                                                                                                               <  2:484957/149‑1 (MQ=255)
                           gtaaaaCGGTGATTTTAAGTGGGGGCTGCTTTGTGGCTTTGGAGCTGGACGCAGGACGGGCAATTGCCCAGGGAACCGGGCTGGCGCTGGAGATTTAAATGGTTTTTTGAAGCGCCGGTTGCAACGCACCCGGCACGTCGCGTTAATCaa                                                                                                                            <  2:1772355/148‑1 (MQ=255)
                            gAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAAt                                                                                                                           >  2:2244542/1‑150 (MQ=255)
                              aaaCGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATaa                                                                                                                         >  2:672481/1‑150 (MQ=255)
                                   gTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAg                                                                                                                    >  2:2559078/1‑150 (MQ=255)
                                   gTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAg                                                                                                                    >  2:1112481/1‑150 (MQ=255)
                                       atttatttGGGGGCTGGTTTGGGGCTTTGGAGCTGGACGCACGAGGGGCATTTGCCCAGGTAACACGGCTGGGGCTGTTTATTTAAATCGTTATTTGAAGCGCCGGTTGCAACGCATCGGGCACGTCGCGTTAATCAATAAACAAGGCgg                                                                                                                <  2:2228487/150‑1 (MQ=255)
                                         tttAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCggg                                                                                                               >  1:1730590/1‑150 (MQ=255)
                                             aTTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATACGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGaa                                                                                                           >  2:1232652/1‑150 (MQ=255)
                                                 ggggcTGCGTTGTGGCTCTGGCGCTGGCCGCCCGCGGGGCTCGTGCCCAGGGAAGCCGGGTGGGGCGGGCGTTTTAAAGGGTTTTTTGCCGCGCGGGTTGCAACGCAGCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGc                                                                                                       <  2:1946523/148‑1 (MQ=255)
                                                   ggcTGCGATGTGGCTATGGAGCTGGACGCACTACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCg                                                                                                     >  2:2426285/1‑150 (MQ=255)
                                                     cTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCCGGGAACACGGCTGGCGCTGGATATTTAACTCGTTATTTGACGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGaa                                                                                                   <  2:1803217/150‑1 (MQ=255)
                                                                  aTGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAt                                                                                      >  2:1632578/1‑150 (MQ=255)
                                                                  aTGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAt                                                                                      >  2:1632625/1‑150 (MQ=255)
                                                                     gtggtGGACGCCCGAGGGGCATTTGCCCTGGTACCCGGGCGGGGGGGGGATTTTTAAAGCGTTTTTTGCCGCGCGGGATGCAACGCATCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAtttt                                                                                   <  1:2683599/146‑1 (MQ=255)
                                                                        cTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTa                                                                                >  2:1585852/1‑150 (MQ=255)
                                                                        cTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTa                                                                                >  2:1215287/1‑150 (MQ=255)
                                                                        cTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTa                                                                                >  2:2653645/1‑150 (MQ=255)
                                                                        cTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTa                                                                                >  2:2195844/1‑150 (MQ=255)
                                                                              gCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAATGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCgtgt                                                                          >  1:1299772/1‑150 (MQ=255)
                                                                              gCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCgtgt                                                                          >  1:2137794/1‑150 (MQ=255)
                                                                              gCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCgtgt                                                                          >  2:2389052/1‑150 (MQ=255)
                                                                                cctccgGGAATTTGCCCTGGGAACCGGGGTGGCGGTTGAGTTTTAAATGGTTATTTGACGCGCGGGATGCACCGCATCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTc                                                                        <  2:979382/146‑1 (MQ=255)
                                                                                acgacgGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTc                                                                        >  2:865766/1‑150 (MQ=255)
                                                                                  cctgggCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCa                                                                      >  1:626287/4‑150 (MQ=255)
                                                                                  cctgggCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCa                                                                      >  2:1129295/4‑150 (MQ=255)
                                                                                   acgGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAg                                                                     >  1:2633222/1‑150 (MQ=255)
                                                                                   acgGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAg                                                                     >  1:2699837/1‑150 (MQ=255)
                                                                                       gCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGtt                                                                 >  2:2323897/1‑150 (MQ=255)
                                                                                       gCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGtt                                                                 >  2:762372/1‑150 (MQ=255)
                                                                                       gCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGtt                                                                 >  2:438885/1‑150 (MQ=255)
                                                                                          atggCCCAGGTACCCGGGCTGGCGCTGTATATTTAATTCTTTTTTTGACGCGCGTGATGCAACGCATCGGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAAt                                                              <  1:2577181/147‑1 (MQ=255)
                                                                                           ttGCCCTGGCAACCCGGGTGGGGCTGTTGATTTAATTGGTTTTTGGACGCGCCGTAGGCAACGCTTCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAAtt                                                             <  2:2357041/150‑1 (MQ=255)
                                                                                           tggCCCTGGGACCCCGGCTGGCGCTGTTCATTTAAATCGTTATTGCAAGCGCCGGCTGCACCGCTTCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAAtt                                                             <  2:2356946/148‑1 (MQ=255)
                                                                                              cccAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTcc                                                          >  2:2712633/1‑150 (MQ=255)
                                                                                               cctggtaaCAGGGCTGGCGCTGGATATTTAAATCTTTATTTGAAGCGCCGGATGCAACGCACCGGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCct                                                         <  2:345854/144‑1 (MQ=255)
                                                                                               cctgggAACCGGGCTGGCGCTGGAGATTTAAATCTTTATTTGACGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCct                                                         <  2:345664/147‑1 (MQ=255)
                                                                                               cctgggAACCCGGGGGGGGCTGGATATTTAAATGGTTATTTGACGCGCCGGTTGAAACGCACCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCct                                                         <  2:1472897/147‑1 (MQ=255)
                                                                                                 aGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCctct                                                       >  1:1480993/1‑150 (MQ=255)
                                                                                                       cccggggggCGCTGTAGTTTTAAAGCTTTTTTTGACGCGCCGGATGAAACGCTCCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGc                                                 <  1:2421937/143‑1 (MQ=255)
                                                                                                       cacGGGTGGCGCTGTAGATTTAAATGTTAATTTGACGCGCCGGCTGCATCGCCTCGGTCACGTCGCGTTAATCTATAAACAAGGCGGGAGAAATGCCGAATAGCGTTGCCATTTTTTTAGCGTGTTCCAGCGTTAATTTCCCCTTCCCGc                                                 <  1:1739853/150‑1 (MQ=255)
                                                                                                       cacGGCGGGCGCTGTATATTTAAATCGTTATTTGACGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGc                                                 <  1:409670/150‑1 (MQ=255)
                                                                                                        acGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGGTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTACAGCGTTAATTTCCTCTTCCCGCt                                                >  1:1575258/1‑150 (MQ=255)
                                                                                                              ggCGCTTGATATTTAAATCGTTATTTGAAGCGCCGGTTGAACCGAATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAAc                                          <  1:2688856/150‑1 (MQ=255)
                                                                                                               gcgcTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAAcg                                         >  1:2440001/1‑150 (MQ=255)
                                                                                                                  cTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATACGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGACGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGt                                      >  2:815723/1‑150 (MQ=255)
                                                                                                                       caTTTAAATCGTTAATTGAACCGCCGCATGAAACGCAACCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGAGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGacac                                 <  2:420199/149‑1 (MQ=255)
                                                                                                                         tttAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCa                               >  2:919853/1‑150 (MQ=255)
                                                                                                                         tttAAATCGTTATTTGAAGCGCCGGATGCAACGCATACCGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATAGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTACTCTTCCCGCTCAAAACGCGTGACACCa                               >  1:1917512/1‑150 (MQ=255)
                                                                                                                         tttAAATCGTTATTTGAAGCGCCGGATGCAACGCATACCGCACGTCGCGTTAATCAAAAAACAAGGCGGGCGAAATGCCGAATCGCGTTGCCAATTTTTTATCGTGTTCCAGCGTTAATTTACTCTTCCCGCTCAAAACGCGTGACACCa                               >  1:1917575/1‑150 (MQ=255)
                                                                                                                          ttAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCAt                              >  2:888308/1‑150 (MQ=255)
                                                                                                                          ttAAATCGTTATTTGAAGCGCCGGATGCAACCCATCCGGCACGTCGCCTTAATAAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCACTTACCGCTCAAAACGCCTGACACCaa                              >  1:2378925/1‑149 (MQ=255)
                                                                                                                           tAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATa                             <  1:669848/150‑1 (MQ=255)
                                                                                                                             aaTCGTTATTTGAAGCGCCGGATGAAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGa                           <  2:687713/150‑1 (MQ=255)
                                                                                                                                 gTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGAtgtt                       >  2:2544431/1‑147 (MQ=255)
                                                                                                                                  ttATTTGACGCGCCGGATGCAACGAATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTAc                      <  2:293309/150‑1 (MQ=255)
                                                                                                                                  ttATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTAc                      <  1:436178/150‑1 (MQ=255)
                                                                                                                                  ttATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTAc                      >  2:633242/1‑150 (MQ=255)
                                                                                                                                  ttATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGATGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTAc                      >  2:2697092/1‑150 (MQ=255)
                                                                                                                                    aTTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTg                    >  2:990092/1‑150 (MQ=255)
                                                                                                                                    aTTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTg                    >  2:133688/1‑150 (MQ=255)
                                                                                                                                      tttaaGCGCGGTATTCAACGAATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGcc                  <  2:848193/147‑1 (MQ=255)
                                                                                                                                        gacgcgcCGGATGCAACGCAGCCGGCACGTCGCGTTAATCAATAACCAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCaa                <  1:1910728/147‑1 (MQ=255)
                                                                                                                                        gAAGCGCCGGATGAAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCaa                <  1:1910848/150‑1 (MQ=255)
                                                                                                                                          aGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAAtt              >  2:941594/1‑150 (MQ=255)
                                                                                                                                           gtgcCGGATGCGTTGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAAttt             >  2:1027503/3‑150 (MQ=255)
                                                                                                                                           gtgcCGGATGCGTTGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAAttt             >  2:1027506/3‑150 (MQ=255)
                                                                                                                                            cgcggtaTGAAACCCATCCGCCACGTAGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTc            <  2:2646043/144‑1 (MQ=255)
                                                                                                                                            cgcCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTc            <  2:2054217/150‑1 (MQ=255)
                                                                                                                                            cgcCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTc            <  1:194100/150‑1 (MQ=255)
                                                                                                                                             gcCGTATGCAACTAATCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTcc           <  1:1903754/150‑1 (MQ=255)
                                                                                                                                              ccGGATGCGTTGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCg          >  2:1515806/1‑150 (MQ=255)
                                                                                                                                               cGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCgg         <  2:1999756/150‑1 (MQ=255)
                                                                                                                                                ggATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGc        >  1:648923/1‑150 (MQ=255)
                                                                                                                                                ggATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGc        >  1:2025225/1‑150 (MQ=255)
                                                                                                                                                 gATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCa       >  2:1206332/1‑150 (MQ=255)
                                                                                                                                                  aTGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAg      >  2:2287953/1‑150 (MQ=255)
                                                                                                                                                  aTGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAg      >  1:2358357/1‑150 (MQ=255)
                                                                                                                                                   tGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGa     >  1:808953/1‑150 (MQ=255)
                                                                                                                                                   tGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGa     >  1:808955/1‑150 (MQ=255)
                                                                                                                                                      aaCGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCg  >  1:833342/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCG  >  NC_000913/3233559‑3233857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: