Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,233,709 A→T 6.2% intergenic (+26/+19) fadH → / ← higA 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked/antitoxinof the HigB‑HigA toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,233,7090AT 6.2% 323.1 / 8.7 117intergenic (+26/+19)fadH/higA2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked/antitoxinof the HigB‑HigA toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base A (62/42);  new base T (3/5);  total (65/52)
Fisher's exact test for biased strand distribution p-value = 2.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 121 total aligned reads displayed.

GCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGG  >  NC_000913/3233560‑3233858
                                                                                                                                                      |                                                                                                                                                     
gcTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCaa                                                                                                                                                       <  1:2676086/150‑1 (MQ=255)
 cgggCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAAc                                                                                                                                                      <  1:1197313/148‑1 (MQ=255)
 cTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAAc                                                                                                                                                      <  1:2376847/150‑1 (MQ=255)
 cTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAAc                                                                                                                                                      <  1:2376846/150‑1 (MQ=255)
    gcgcAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCa                                                                                                                                                   >  2:669848/1‑150 (MQ=255)
    gcgcAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCa                                                                                                                                                   >  1:1343276/1‑150 (MQ=255)
    gcgcAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCa                                                                                                                                                   >  1:293309/1‑150 (MQ=255)
       cAACCGCTGTTTGATAGCGGGAAAACGGTGCATTTAATGGGCGGCTGCGATGTGGCTATGGAGCTGGACGCTCGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATcc                                                                                                                                                <  2:100407/150‑1 (MQ=255)
       cAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAGCACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATcc                                                                                                                                                <  2:100404/150‑1 (MQ=255)
       cAACCGCTGATTGATAGCGGGAAAACGGAGCAATTAAATGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATcc                                                                                                                                                <  2:460683/150‑1 (MQ=255)
         aCCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCGTTGCATCCgg                                                                                                                                              <  2:2150072/150‑1 (MQ=255)
            gCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCGTTGCATCCGGcgc                                                                                                                                           <  2:1732823/150‑3 (MQ=255)
             cTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACg                                                                                                                                          >  2:1903754/1‑150 (MQ=255)
              tgattgatAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGt                                                                                                                                         >  1:2341635/1‑150 (MQ=255)
                  tgatAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGGcgcg                                                                                                                                     >  1:1335556/1‑150 (MQ=255)
                      cgCGGGAACACGGTGCCTTCACGTGGCGGCTGGGATGGGGCAATGGAGCTGGCCGCCCGACGGGCACTGGCCCAGGGAACCCGGCTGGGGCGGGAGATTTAAATCGTTATTTGACGCGCCGGATGCACCGCTTCCGGCACGTCGCGTTaa                                                                                                                                 <  2:1847884/149‑1 (MQ=255)
                      aGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTaa                                                                                                                                 >  1:1955835/1‑150 (MQ=255)
                        ggggTAAAAGGTGGATTTTATTTGGCGGCTGCGTTGGGGCTTTGGCGCTGGACGCACGCGGGGAATTTGCCCTGGGAACCGGGGTGGGGCTGTAGATTTAAATGGTTTTTTGACGCGCCGGTTGCAAGGCATCCGGCACGTCGCGTTAAt                                                                                                                                <  2:484957/149‑1 (MQ=255)
                          gtaaaaCGGTGATTTTAAGTGGGGGCTGCTTTGTGGCTTTGGAGCTGGACGCAGGACGGGCAATTGCCCAGGGAACCGGGCTGGCGCTGGAGATTTAAATGGTTTTTTGAAGCGCCGGTTGCAACGCACCCGGCACGTCGCGTTAATCaa                                                                                                                             <  2:1772355/148‑1 (MQ=255)
                           gAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAAt                                                                                                                            >  2:2244542/1‑150 (MQ=255)
                             aaaCGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATaa                                                                                                                          >  2:672481/1‑150 (MQ=255)
                                cGGTGCTTTTATGTGGCGGCTGCGTTGTGGCTATGGAGCTGGACGCACGACGGGAATTGGCCCTGGGAACACGGGTGGCGCTGGATATTTAAATCGTTATTTGACGCGCCGGTTGCAACGCAACGGGCACGTCGCGTTAATCAATAAACa                                                                                                                       <  2:1335556/150‑1 (MQ=255)
                                  gTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAg                                                                                                                     >  2:1112481/1‑150 (MQ=255)
                                  gTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAg                                                                                                                     >  2:1112557/1‑150 (MQ=255)
                                  gTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAg                                                                                                                     >  2:2559078/1‑150 (MQ=255)
                                      atttatttGGGGGCTGGTTTGGGGCTTTGGAGCTGGACGCACGAGGGGCATTTGCCCAGGTAACACGGCTGGGGCTGTTTATTTAAATCGTTATTTGAAGCGCCGGTTGCAACGCATCGGGCACGTCGCGTTAATCAATAAACAAGGCgg                                                                                                                 <  2:2228487/150‑1 (MQ=255)
                                        tttAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCggg                                                                                                                >  1:1730590/1‑150 (MQ=255)
                                            aTTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATACGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGaa                                                                                                            >  2:1232652/1‑150 (MQ=255)
                                                ggggcTGCGTTGTGGCTCTGGCGCTGGCCGCCCGCGGGGCTCGTGCCCAGGGAAGCCGGGTGGGGCGGGCGTTTTAAAGGGTTTTTTGCCGCGCGGGTTGCAACGCAGCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGc                                                                                                        <  2:1946523/148‑1 (MQ=255)
                                                             ggCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGc                                                                                           >  1:1366029/1‑150 (MQ=255)
                                                                 aTGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAt                                                                                       >  2:1632578/1‑150 (MQ=255)
                                                                 aTGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAt                                                                                       >  2:1632613/1‑150 (MQ=255)
                                                                 aTGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAt                                                                                       >  2:1632625/1‑150 (MQ=255)
                                                                    gtggtGGACGCCCGAGGGGCATTTGCCCTGGTACCCGGGCGGGGGGGGGATTTTTAAAGCGTTTTTTGCCGCGCGGGATGCAACGCATCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAtttt                                                                                    <  1:2683599/146‑1 (MQ=255)
                                                                       cgggccGCACGCGGGGCAATGGCCCCGGCAACCCGGCTGGCGCTGCATATTTAAATGGTTATTCGACGCGCGGGATGCAACGAAACCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTa                                                                                 <  2:628023/145‑1 (MQ=255)
                                                                       cTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTa                                                                                 >  2:2195844/1‑150 (MQ=255)
                                                                       cTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTa                                                                                 >  2:2653645/1‑150 (MQ=255)
                                                                             gCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAATGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCgtgt                                                                           >  1:1299772/1‑150 (MQ=255)
                                                                             gCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCgtgt                                                                           >  2:2389052/1‑150 (MQ=255)
                                                                             gCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCgtgt                                                                           >  1:2137794/1‑150 (MQ=255)
                                                                               cctccgGGAATTTGCCCTGGGAACCGGGGTGGCGGTTGAGTTTTAAATGGTTATTTGACGCGCGGGATGCACCGCATCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTc                                                                         <  2:979382/146‑1 (MQ=255)
                                                                               acgacgGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTc                                                                         >  2:865766/1‑150 (MQ=255)
                                                                               acgacgGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTc                                                                         <  2:2698192/150‑1 (MQ=255)
                                                                                 cctgggCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCa                                                                       >  2:1129295/4‑150 (MQ=255)
                                                                                 cctgggCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCa                                                                       >  1:626287/4‑150 (MQ=255)
                                                                                  acgGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAg                                                                      >  1:2633222/1‑150 (MQ=255)
                                                                                      gCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGtt                                                                  >  2:438885/1‑150 (MQ=255)
                                                                                      gCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGtt                                                                  >  2:2323897/1‑150 (MQ=255)
                                                                                      gCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGtt                                                                  >  2:762372/1‑150 (MQ=255)
                                                                                         atggCCCAGGTACCCGGGCTGGCGCTGTATATTTAATTCTTTTTTTGACGCGCGTGATGCAACGCATCGGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAAt                                                               <  1:2577181/147‑1 (MQ=255)
                                                                                          ttGCCCTGGCAACCCGGGTGGGGCTGTTGATTTAATTGGTTTTTGGACGCGCCGTAGGCAACGCTTCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAAtt                                                              <  2:2357041/150‑1 (MQ=255)
                                                                                          tggCCCTGGGACCCCGGCTGGCGCTGTTCATTTAAATCGTTATTGCAAGCGCCGGCTGCACCGCTTCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAAtt                                                              <  2:2356946/148‑1 (MQ=255)
                                                                                             cccAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTcc                                                           >  2:2712633/1‑150 (MQ=255)
                                                                                              cctgggAACCGGGCTGGCGCTGGAGATTTAAATCTTTATTTGACGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCct                                                          <  2:345664/147‑1 (MQ=255)
                                                                                              cctgggAACCCGGGGGGGGCTGGATATTTAAATGGTTATTTGACGCGCCGGTTGAAACGCACCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCct                                                          <  2:1472897/147‑1 (MQ=255)
                                                                                                      cccggggggCGCTGTAGTTTTAAAGCTTTTTTTGACGCGCCGGATGAAACGCTCCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGc                                                  <  1:2421937/143‑1 (MQ=255)
                                                                                                      cacGGGTGGCGCTGTAGATTTAAATGTTAATTTGACGCGCCGGCTGCATCGCCTCGGTCACGTCGCGTTAATCTATAAACAAGGCGGGAGAAATGCCGAATAGCGTTGCCATTTTTTTAGCGTGTTCCAGCGTTAATTTCCCCTTCCCGc                                                  <  1:1739853/150‑1 (MQ=255)
                                                                                                      cacGGCGGGCGCTGTATATTTAAATCGTTATTTGACGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGc                                                  <  1:409670/150‑1 (MQ=255)
                                                                                                       acGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGGTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTACAGCGTTAATTTCCTCTTCCCGCt                                                 >  1:1575258/1‑150 (MQ=255)
                                                                                                           gtGGCGCTGTAGTTTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCaaa                                             <  2:384579/149‑1 (MQ=255)
                                                                                                             ggCGCTTGATATTTAAATCGTTATTTGAAGCGCCGGTTGAACCGAATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAAc                                           <  1:2688856/150‑1 (MQ=255)
                                                                                                               ggcTGTATATTTAAATTGTTATTTGACGCGCCGTATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAAcgc                                         <  2:1854796/149‑1 (MQ=255)
                                                                                                                 cTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATACGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGACGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGt                                       >  2:815723/1‑150 (MQ=255)
                                                                                                                      caTTTAAATCGTTAATTGAACCGCCGCATGAAACGCAACCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGAGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGacac                                  <  2:420199/149‑1 (MQ=255)
                                                                                                                        tttAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCa                                >  2:919853/1‑150 (MQ=255)
                                                                                                                        tttAAATCGTTATTTGAAGCGCCGGATGCAACGCATACCGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATAGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTACTCTTCCCGCTCAAAACGCGTGACACCa                                >  1:1917512/1‑150 (MQ=255)
                                                                                                                        tttAAATCGTTATTTGAAGCGCCGGATGCAACGCATACCGCACGTCGCGTTAATCAAAAAACAAGGCGGGCGAAATGCCGAATCGCGTTGCCAATTTTTTATCGTGTTCCAGCGTTAATTTACTCTTCCCGCTCAAAACGCGTGACACCa                                >  1:1917575/1‑150 (MQ=255)
                                                                                                                         ttAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCAt                               >  2:888308/1‑150 (MQ=255)
                                                                                                                         ttAAATCGTTATTTGAAGCGCCGGATGCAACCCATCCGGCACGTCGCCTTAATAAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCACTTACCGCTCAAAACGCCTGACACCaa                               >  1:2378925/1‑149 (MQ=255)
                                                                                                                          tAAATCGTTTTTTGACGCGCCGTTTGCAAGGCTTCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATa                              <  2:2343102/150‑1 (MQ=255)
                                                                                                                          tAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATa                              <  1:669848/150‑1 (MQ=255)
                                                                                                                            aaTCGTTATTTGAAGCGCCGGATGAAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGa                            <  2:687713/150‑1 (MQ=255)
                                                                                                                              ccGTTATTTGACGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGAtt                          <  1:306030/149‑1 (MQ=255)
                                                                                                                                gTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGAtgtt                        >  2:2544431/1‑147 (MQ=255)
                                                                                                                                 ttATTTGACGCGCCGGATGCAACGAATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTAc                       <  2:293309/150‑1 (MQ=255)
                                                                                                                                 ttATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTAc                       >  2:633242/1‑150 (MQ=255)
                                                                                                                                 ttATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTAc                       <  1:436178/150‑1 (MQ=255)
                                                                                                                                   aTTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTg                     >  2:990092/1‑150 (MQ=255)
                                                                                                                                   aTTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTg                     >  2:133688/1‑150 (MQ=255)
                                                                                                                                     tttaaGCGCGGTATTCAACGAATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGcc                   <  2:848193/147‑1 (MQ=255)
                                                                                                                                       gacgcgcGGGATGCAACCCCGCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGGTCCAGCGTTAAGTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCaa                 <  1:1910709/147‑1 (MQ=255)
                                                                                                                                       gAAGCGCCGGATGAAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCaa                 <  1:1910848/150‑1 (MQ=255)
                                                                                                                                         aGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAAtt               >  2:941594/1‑150 (MQ=255)
                                                                                                                                          gtgcCGGATGCGTTGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAAttt              >  2:1027503/3‑150 (MQ=255)
                                                                                                                                           cgcggtaTGAAACCCATCCGCCACGTAGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTc             <  2:2646043/144‑1 (MQ=255)
                                                                                                                                           cgcCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTc             <  2:2054217/150‑1 (MQ=255)
                                                                                                                                           cgcCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTc             <  1:194069/150‑1 (MQ=255)
                                                                                                                                           cgcCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTc             <  1:194100/150‑1 (MQ=255)
                                                                                                                                            gcCGTATGCAACTAATCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTcc            <  1:1903754/150‑1 (MQ=255)
                                                                                                                                            gcCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTcc            <  1:672481/150‑1 (MQ=255)
                                                                                                                                             ccGGATGCGTTGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCg           >  2:1515806/1‑150 (MQ=255)
                                                                                                                                              cGGTTGCACCGCATCCGGCACGTCGCGTTTATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCgg          <  2:2521226/150‑1 (MQ=255)
                                                                                                                                               tgATGCATCGAAGCCGCAACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCATTTTCCGGc         <  1:2094096/149‑1 (MQ=255)
                                                                                                                                               ggATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGc         >  1:2025225/1‑150 (MQ=255)
                                                                                                                                               ggATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGc         >  1:942944/1‑150 (MQ=255)
                                                                                                                                                gATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCa        >  2:1206332/1‑150 (MQ=255)
                                                                                                                                                  tGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGa      >  1:808953/1‑150 (MQ=255)
                                                                                                                                                  tGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGa      >  1:808955/1‑150 (MQ=255)
                                                                                                                                                     aaCGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCg   >  1:833342/1‑150 (MQ=255)
                                                                                                                                                      aCGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCgg  >  2:1151999/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGG  >  NC_000913/3233560‑3233858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: