Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,233,710 C→T 5.3% intergenic (+27/+18) fadH → / ← higA 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked/antitoxinof the HigB‑HigA toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,233,7100CT 5.3% 364.3 / 7.4 116intergenic (+27/+18)fadH/higA2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked/antitoxinof the HigB‑HigA toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base C (64/44);  new base T (3/3);  total (67/49)
Fisher's exact test for biased strand distribution p-value = 6.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.69e-01

Only 100 of 119 total aligned reads displayed.

CTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGG  >  NC_000913/3233561‑3233858
                                                                                                                                                     |                                                                                                                                                    
cgggCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAAc                                                                                                                                                      <  1:1197313/148‑1 (MQ=255)
cTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAAc                                                                                                                                                      <  1:2376846/150‑1 (MQ=255)
   gcgcAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCa                                                                                                                                                   >  1:195095/1‑150 (MQ=255)
   gcgcAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCa                                                                                                                                                   >  1:1343276/1‑150 (MQ=255)
   gcgcAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCa                                                                                                                                                   >  2:669848/1‑150 (MQ=255)
   gcgcAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCa                                                                                                                                                   >  1:293309/1‑150 (MQ=255)
      cAACCGCTGTTTGATAGCGGGAAAACGGTGCATTTAATGGGCGGCTGCGATGTGGCTATGGAGCTGGACGCTCGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATcc                                                                                                                                                <  2:100407/150‑1 (MQ=255)
      cAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAGCACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATcc                                                                                                                                                <  2:100404/150‑1 (MQ=255)
      cAACCGCTGATTGATAGCGGGAAAACGGAGCAATTAAATGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATcc                                                                                                                                                <  2:460683/150‑1 (MQ=255)
        aCCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCGTTGCATCCgg                                                                                                                                              <  2:2150072/150‑1 (MQ=255)
        aCCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCGTTGCATCCgg                                                                                                                                              <  2:511400/150‑1 (MQ=255)
           gCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCGTTGCATCCGGcgc                                                                                                                                           <  2:1732823/150‑3 (MQ=255)
            cTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACg                                                                                                                                          >  2:1903754/1‑150 (MQ=255)
             tgattgatAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGt                                                                                                                                         >  1:2341635/1‑150 (MQ=255)
                 tgatAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGGcgcg                                                                                                                                     >  1:1335556/1‑150 (MQ=255)
                         gtaaaaCGGTGATTTTAAGTGGGGGCTGCTTTGTGGCTTTGGAGCTGGACGCAGGACGGGCAATTGCCCAGGGAACCGGGCTGGCGCTGGAGATTTAAATGGTTTTTTGAAGCGCCGGTTGCAACGCACCCGGCACGTCGCGTTAATCaa                                                                                                                             <  2:1772355/148‑1 (MQ=255)
                          gAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAAt                                                                                                                            >  2:2244542/1‑150 (MQ=255)
                            aaaCGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATaa                                                                                                                          >  2:672481/1‑150 (MQ=255)
                               cGGTGCTTTTATGTGGCGGCTGCGTTGTGGCTATGGAGCTGGACGCACGACGGGAATTGGCCCTGGGAACACGGGTGGCGCTGGATATTTAAATCGTTATTTGACGCGCCGGTTGCAACGCAACGGGCACGTCGCGTTAATCAATAAACa                                                                                                                       <  2:1335556/150‑1 (MQ=255)
                                 gTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAg                                                                                                                     >  2:1112557/1‑150 (MQ=255)
                                     atttatttGGGGGCTGGTTTGGGGCTTTGGAGCTGGACGCACGAGGGGCATTTGCCCAGGTAACACGGCTGGGGCTGTTTATTTAAATCGTTATTTGAAGCGCCGGTTGCAACGCATCGGGCACGTCGCGTTAATCAATAAACAAGGCgg                                                                                                                 <  2:2228487/150‑1 (MQ=255)
                                      tttAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCggg                                                                                                                >  1:1730590/1‑150 (MQ=255)
                                              ggggcTGCGTTGTGGCTCTGGCGCTGGCCGCCCGCGGGGCTCGTGCCCAGGGAAGCCGGGTGGGGCGGGCGTTTTAAAGGGTTTTTTGCCGCGCGGGTTGCAACGCAGCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGc                                                                                                        <  2:1946523/148‑1 (MQ=255)
                                                ggcTGCGATGTGGCTATGGAGCTGGACGCACTACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCg                                                                                                      >  2:2426285/1‑150 (MQ=255)
                                                  cTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCCGGGAACACGGCTGGCGCTGGATATTTAACTCGTTATTTGACGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGaa                                                                                                    <  2:1803217/150‑1 (MQ=255)
                                                           ggCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGc                                                                                           >  1:1366029/1‑150 (MQ=255)
                                                               aTGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAt                                                                                       >  2:1632625/1‑150 (MQ=255)
                                                               aTGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAt                                                                                       >  2:1632613/1‑150 (MQ=255)
                                                                     cgggccGCACGCGGGGCAATGGCCCCGGCAACCCGGCTGGCGCTGCATATTTAAATGGTTATTCGACGCGCGGGATGCAACGAAACCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTa                                                                                 <  2:628023/145‑1 (MQ=255)
                                                                     cTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTa                                                                                 >  2:1215287/1‑150 (MQ=255)
                                                                     cTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTa                                                                                 >  2:2653645/1‑150 (MQ=255)
                                                                     cTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTa                                                                                 >  2:1585852/1‑150 (MQ=255)
                                                                           gCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAATGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCgtgt                                                                           >  1:1299772/1‑150 (MQ=255)
                                                                           gCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCgtgt                                                                           >  2:2389052/1‑150 (MQ=255)
                                                                           gCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCgtgt                                                                           >  1:2137794/1‑150 (MQ=255)
                                                                             cctccgGGAATTTGCCCTGGGAACCGGGGTGGCGGTTGAGTTTTAAATGGTTATTTGACGCGCGGGATGCACCGCATCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTc                                                                         <  2:979382/146‑1 (MQ=255)
                                                                             acgacgGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTc                                                                         >  2:865766/1‑150 (MQ=255)
                                                                             acgacgGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTc                                                                         <  2:2698192/150‑1 (MQ=255)
                                                                               cctgggCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCa                                                                       >  1:626287/4‑150 (MQ=255)
                                                                               cctgggCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCa                                                                       >  2:1129295/4‑150 (MQ=255)
                                                                                acgGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAg                                                                      >  1:2699837/1‑150 (MQ=255)
                                                                                acgGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAg                                                                      >  1:2633222/1‑150 (MQ=255)
                                                                                    gCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGtt                                                                  >  2:2323897/1‑150 (MQ=255)
                                                                                    gCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGtt                                                                  >  2:762372/1‑150 (MQ=255)
                                                                                    gCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGtt                                                                  >  2:438885/1‑150 (MQ=255)
                                                                                       atggCCCAGGTACCCGGGCTGGCGCTGTATATTTAATTCTTTTTTTGACGCGCGTGATGCAACGCATCGGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAAt                                                               <  1:2577181/147‑1 (MQ=255)
                                                                                        ttGCCCTGGCAACCCGGGTGGGGCTGTTGATTTAATTGGTTTTTGGACGCGCCGTAGGCAACGCTTCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAAtt                                                              <  2:2357041/150‑1 (MQ=255)
                                                                                           cccAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTcc                                                           >  2:2712633/1‑150 (MQ=255)
                                                                                            cctggtaaCAGGGCTGGCGCTGGATATTTAAATCTTTATTTGAAGCGCCGGATGCAACGCACCGGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCct                                                          <  2:345854/144‑1 (MQ=255)
                                                                                            cctgggAACCGGGCTGGCGCTGGAGATTTAAATCTTTATTTGACGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCct                                                          <  2:345664/147‑1 (MQ=255)
                                                                                            cctgggAACCCGGGGGGGGCTGGATATTTAAATGGTTATTTGACGCGCCGGTTGAAACGCACCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCct                                                          <  2:1472897/147‑1 (MQ=255)
                                                                                              aGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCctct                                                        >  1:1480993/1‑150 (MQ=255)
                                                                                                    cccggggggCGCTGTAGTTTTAAAGCTTTTTTTGACGCGCCGGATGAAACGCTCCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGc                                                  <  1:2421937/143‑1 (MQ=255)
                                                                                                    cacGGGTGGCGCTGTAGATTTAAATGTTAATTTGACGCGCCGGCTGCATCGCCTCGGTCACGTCGCGTTAATCTATAAACAAGGCGGGAGAAATGCCGAATAGCGTTGCCATTTTTTTAGCGTGTTCCAGCGTTAATTTCCCCTTCCCGc                                                  <  1:1739853/150‑1 (MQ=255)
                                                                                                    cacGGCGGGCGCTGTATATTTAAATCGTTATTTGACGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGc                                                  <  1:409670/150‑1 (MQ=255)
                                                                                                         gtGGCGCTGTAGTTTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCaaa                                             <  2:384579/149‑1 (MQ=255)
                                                                                                           ggCGCTTGATATTTAAATCGTTATTTGAAGCGCCGGTTGAACCGAATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAAc                                           <  1:2688856/150‑1 (MQ=255)
                                                                                                            gcgcTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAAcg                                          >  1:2440001/1‑150 (MQ=255)
                                                                                                             ggcTGTATATTTAAATTGTTATTTGACGCGCCGTATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAAcgc                                         <  2:1854796/149‑1 (MQ=255)
                                                                                                               cTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATACGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGACGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGt                                       >  2:815723/1‑150 (MQ=255)
                                                                                                                    caTTTAAATCGTTAATTGAACCGCCGCATGAAACGCAACCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGAGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGacac                                  <  2:420199/149‑1 (MQ=255)
                                                                                                                      tttAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCa                                >  2:919803/1‑150 (MQ=255)
                                                                                                                      tttAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCa                                >  2:919853/1‑150 (MQ=255)
                                                                                                                      tttAAATCGTTATTTGAAGCGCCGGATGCAACGCATACCGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATAGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTACTCTTCCCGCTCAAAACGCGTGACACCa                                >  1:1917512/1‑150 (MQ=255)
                                                                                                                      tttAAATCGTTATTTGAAGCGCCGGATGCAACGCATACCGCACGTCGCGTTAATCAAAAAACAAGGCGGGCGAAATGCCGAATCGCGTTGCCAATTTTTTATCGTGTTCCAGCGTTAATTTACTCTTCCCGCTCAAAACGCGTGACACCa                                >  1:1917575/1‑150 (MQ=255)
                                                                                                                       ttAAATCGTTATTTGAAGCGCCGGATGCAACCCATCCGGCACGTCGCCTTAATAAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCACTTACCGCTCAAAACGCCTGACACCaa                               >  1:2378925/1‑149 (MQ=255)
                                                                                                                        tAAATCGTTTTTTGACGCGCCGTTTGCAAGGCTTCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATa                              <  2:2343102/150‑1 (MQ=255)
                                                                                                                        tAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATa                              <  1:669848/150‑1 (MQ=255)
                                                                                                                            ccGTTATTTGACGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGAtt                          <  1:306030/149‑1 (MQ=255)
                                                                                                                              gTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGAtgtt                        >  2:2544431/1‑147 (MQ=255)
                                                                                                                               ttATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTAc                       >  2:633242/1‑150 (MQ=255)
                                                                                                                               ttATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGATGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTAc                       >  2:2697092/1‑150 (MQ=255)
                                                                                                                                 aTTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTg                     >  2:990092/1‑150 (MQ=255)
                                                                                                                                 aTTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTg                     >  2:133688/1‑150 (MQ=255)
                                                                                                                                   tttaaGCGCGGTATTCAACGAATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGcc                   <  2:848193/147‑1 (MQ=255)
                                                                                                                                     gacgcgcGGGATGCAACCCCGCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGGTCCAGCGTTAAGTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCaa                 <  1:1910709/147‑1 (MQ=255)
                                                                                                                                     gacgcgcCGGATGCAACGCAGCCGGCACGTCGCGTTAATCAATAACCAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCaa                 <  1:1910728/147‑1 (MQ=255)
                                                                                                                                       aGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAAtt               >  2:941594/1‑150 (MQ=255)
                                                                                                                                        gtgcCGGATGCGTTGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAAttt              >  2:1027506/3‑150 (MQ=255)
                                                                                                                                        gtgcCGGATGCGTTGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAAttt              >  2:1027503/3‑150 (MQ=255)
                                                                                                                                         cgcggtaTGAAACCCATCCGCCACGTAGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTc             <  2:2646043/144‑1 (MQ=255)
                                                                                                                                         cgcCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTc             <  2:2054217/150‑1 (MQ=255)
                                                                                                                                         cgcCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTc             <  1:194100/150‑1 (MQ=255)
                                                                                                                                         cgcCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTc             <  1:194069/150‑1 (MQ=255)
                                                                                                                                         cgcCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTc             >  2:737226/1‑150 (MQ=255)
                                                                                                                                          gcCGTATGCAACTAATCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTcc            <  1:1903754/150‑1 (MQ=255)
                                                                                                                                          gcCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTcc            <  1:672481/150‑1 (MQ=255)
                                                                                                                                           ccGGATGCGTTGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCg           >  2:1515806/1‑150 (MQ=255)
                                                                                                                                            cGGTTGCACCGCATCCGGCACGTCGCGTTTATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCgg          <  2:2521226/150‑1 (MQ=255)
                                                                                                                                            cGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCgg          <  2:1999756/150‑1 (MQ=255)
                                                                                                                                             tgATGCATCGAAGCCGCAACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCATTTTCCGGc         <  1:2094096/149‑1 (MQ=255)
                                                                                                                                             ggATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGc         >  1:942944/1‑150 (MQ=255)
                                                                                                                                             ggATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGc         >  1:2025225/1‑150 (MQ=255)
                                                                                                                                             ggATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGc         >  1:648923/1‑150 (MQ=255)
                                                                                                                                              gATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCa        >  2:1206332/1‑150 (MQ=255)
                                                                                                                                               aTGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAg       >  1:2358357/1‑150 (MQ=255)
                                                                                                                                               aTGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAg       >  2:2287953/1‑150 (MQ=255)
                                                                                                                                                tGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGa      >  1:808953/1‑150 (MQ=255)
                                                                                                                                                   aaCGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCg   >  1:833342/1‑150 (MQ=255)
                                                                                                                                                    aCGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCgg  >  2:1151999/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGG  >  NC_000913/3233561‑3233858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: