Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,392,138 A→C 7.9% intergenic (‑110/+320) tldD ← / ← yhdP putative peptidase/DUF3971‑AsmA2 domains protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,392,1380AC 7.9% 498.1 / 29.9 179intergenic (‑110/+320)tldD/yhdPputative peptidase/DUF3971‑AsmA2 domains protein
Reads supporting (aligned to +/- strand):  ref base A (85/78);  new base C (3/12);  total (88/91)
Fisher's exact test for biased strand distribution p-value = 2.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 184 total aligned reads displayed.

CGCCGCTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGA  >  NC_000913/3391990‑3392287
                                                                                                                                                    |                                                                                                                                                     
cgccgcTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAg                                                                                                                                                      <  1:743835/150‑1 (MQ=255)
    gcTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATGCGACGc                                                                                                                                                  >  1:2195124/1‑150 (MQ=255)
    gcTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATGCGACGc                                                                                                                                                  >  1:1708344/1‑150 (MQ=255)
       aGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGcaca                                                                                                                                               <  1:775816/150‑1 (MQ=255)
        gCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAg                                                                                                                                              >  2:1760234/1‑150 (MQ=255)
            ttGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTc                                                                                                                                          <  1:1214838/150‑1 (MQ=255)
            ttGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTc                                                                                                                                          <  1:826810/150‑1 (MQ=255)
            ttGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTc                                                                                                                                          <  1:1610382/150‑1 (MQ=255)
            ttGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTc                                                                                                                                          <  2:1916348/150‑1 (MQ=255)
             tGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCg                                                                                                                                         <  2:1993766/150‑1 (MQ=255)
                  aCTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATct                                                                                                                                    >  2:2713523/1‑149 (MQ=255)
                   cTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGCTGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGGTAGTAGGCCTGATCCGACGCACAGCCTCGcagcag                                                                                                                                   >  2:2365339/1‑150 (MQ=255)
                   cTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATGCGACGCTGTGCGTCTTATCAg                                                                                                                                   <  1:46708/150‑1 (MQ=38)
                   cTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATGCGACGCTGTGCGTCTTATCAg                                                                                                                                   <  1:62515/150‑1 (MQ=38)
                    ttACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATGCGACGCTGTGCGTCTTATCAgg                                                                                                                                  <  2:2498308/150‑1 (MQ=38)
                    ttACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATGCGACGCTGTGCGTCTTATCAgg                                                                                                                                  <  1:2141031/150‑1 (MQ=38)
                    ttACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAgg                                                                                                                                  >  1:2108126/1‑150 (MQ=255)
                    ttACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTGTGTAGGCCTGATAAGACGCACAGCGTCGCATCAgg                                                                                                                                  >  1:168966/1‑150 (MQ=255)
                     tACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATGCGACGCTGTGCGTCTTATCAGGc                                                                                                                                 <  2:1708344/150‑1 (MQ=37)
                     tACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATGCGACGCTGTGCGTCTTATCAGGc                                                                                                                                 <  2:2195124/150‑1 (MQ=37)
                      aCCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCa                                                                                                                                >  2:2085197/1‑150 (MQ=255)
                       ccAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCac                                                                                                                               >  2:1105048/1‑149 (MQ=255)
                        cAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCacc                                                                                                                              >  1:175433/1‑148 (MQ=255)
                        cAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGGAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATGCGACGCTGTGCGTCTTATCAGGccta                                                                                                                              <  2:746836/150‑4 (MQ=37)
                         aGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCAATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACg                                                                                                                             >  1:2142206/1‑150 (MQ=255)
                          ggTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACgg                                                                                                                            <  2:1680657/150‑1 (MQ=255)
                          ggTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACgg                                                                                                                            <  2:2180940/150‑1 (MQ=255)
                           gTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGc                                                                                                                           >  2:1446891/1‑150 (MQ=255)
                            ttAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATAAGGCAACGGCt                                                                                                                          >  1:2015914/1‑150 (MQ=255)
                                  cTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGATGCACAGCGTCGCATCAGGCAACGGCTGTCGGa                                                                                                                    <  2:2049748/150‑1 (MQ=255)
                                      tCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGAAGCACAGCGTCGCTTCAGGCAACGGCTGTCGGATgcg                                                                                                                >  1:1664557/1‑150 (MQ=255)
                                       ggTTTTTGCTACTCGTTTGTTACTGCCGTCGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATgcgg                                                                                                               <  2:252999/149‑1 (MQ=255)
                                        gTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATgcggc                                                                                                              >  1:2328614/1‑150 (MQ=255)
                                           tttGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGt                                                                                                                                           >  1:2442223/1‑118 (MQ=255)
                                             tGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTaaa                                                                                                         <  2:427530/150‑1 (MQ=255)
                                                tACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGc                                                                                                      <  2:832645/150‑1 (MQ=255)
                                                tACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGc                                                                                                      <  2:692958/150‑1 (MQ=255)
                                                 aCTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGcc                                                                                                     <  2:197561/150‑1 (MQ=255)
                                                      ttagttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATc                                                                                                >  1:2504453/1‑150 (MQ=255)
                                                         gttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGGTAGTAGGCCTTATAAGACGCACAGCGTCGCATCAGGCAACGGCTGACGGATGCGCCGTAAACGCCTTATCCGa                                                                                             >  2:1112523/1‑150 (MQ=18)
                                                              tGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTTTAGCCCTTATAAGACGCACGGCGTCCCATCAGGCAACGCCTCACGGAGCCGGCGTAAACGCCTAAACCGACCtcc                                                                                        >  2:1176441/1‑148 (MQ=25)
                                                              tGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTTGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGAGGCGGCGTAAACGCCTTGTCCGACCTAc                                                                                        >  2:1735423/1‑150 (MQ=255)
                                                              tGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACATCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTAc                                                                                        >  2:2428968/1‑150 (MQ=255)
                                                              tGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTAc                                                                                        <  2:183239/150‑1 (MQ=255)
                                                              tGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGACGGATGCGGCGTAAACGCCATATCCGACCTAc                                                                                        >  2:1282456/1‑150 (MQ=255)
                                                               gCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACg                                                                                       <  2:2453519/150‑1 (MQ=255)
                                                                cAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACgg                                                                                      >  1:38937/1‑150 (MQ=255)
                                                                    agagTATTTTTTCGGGCTGCCGGTTGCGGCTTTACCGCCCTACCCAGCCTACGGTTTTGTCCCGGTTTGTCGGCCTGATAAGCCGCACCGCGTCGCACCCGGCCACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTAt                                                                                  <  1:2399438/150‑1 (MQ=17)
                                                                     gagGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTGATg                                                                                 >  2:2623700/1‑150 (MQ=255)
                                                                             tttcgggCTGCCGGAGGGGGCGTGACCGCCCTATCCCGCCTCCGGTTTTGTCCGGTTTTGTCGGCCTTATAAGACGCACCGCGTCGCACCCGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGttt                                                                         <  1:2534771/145‑1 (MQ=14)
                                                                             tttACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGttt                                                                         <  2:1954208/150‑1 (MQ=255)
                                                                                aCGGCTGCCGGCTGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTa                                                                      <  2:352950/150‑1 (MQ=255)
                                                                                aCGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTa                                                                      <  2:1932591/150‑1 (MQ=255)
                                                                                  ggCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAgg                                                                    <  2:2108126/150‑1 (MQ=255)
                                                                                   ggtGCCGGTTGCGGCGTGAACGCCCTATCCAGCCTACGGTTTTGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGc                                                                   <  1:1678891/148‑1 (MQ=255)
                                                                                   gCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGc                                                                   <  2:1519004/150‑1 (MQ=255)
                                                                                   gCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGc                                                                   <  2:873304/150‑1 (MQ=255)
                                                                                    cTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGcc                                                                  >  1:2026953/1‑150 (MQ=255)
                                                                                    cTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGGTGGTTGGCCTGATGAGACGCAAAGCGTCGCAGCTGGCCCCGGCTGCCGGATGCGGCGTAAACGACGTAACCGACCTCCGGTTATGAGCCGAGTGGAGGcc                                                                  >  1:2026940/1‑150 (MQ=14)
                                                                                     tGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                                 <  2:1680884/150‑1 (MQ=255)
                                                                                     ggCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                                 <  2:840973/149‑1 (MQ=255)
                                                                                       ccGGATGCGGCGTGACCGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGa                                                               <  1:2656146/150‑1 (MQ=255)
                                                                                        cGGAGGCGGCGTGAACGCCCTACCCCGCCTACGGTTTTGTGCCGGTTTGTCGGCCTGATAAGACGCACCGCGTCGCATCAGGCCACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGAt                                                              <  1:1242583/150‑1 (MQ=14)
                                                                                          gaggcggcgTGAACGCCCTATCCAGCCTACGGTTTTGTTCCGGTTTGTAGGCCTGATAAGACGCACCGCGTCGCATCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATaa                                                            <  1:1381938/147‑1 (MQ=255)
                                                                                           aTGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTGTGTAGGCCTGATAAg                                                           >  1:995335/1‑150 (MQ=255)
                                                                                            cgcggcgACAACGCCCAACCCACCCTACGGTTATGTACCGCTTTTTAGGCCTCATAACACCCACACCCACCCAACAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGa                                                          <  1:697068/149‑1 (MQ=14)
                                                                                             gcggcgTGACCGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGAc                                                         <  2:733330/150‑1 (MQ=255)
                                                                                             gcggcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGAc                                                         >  1:1974956/1‑150 (MQ=255)
                                                                                                gcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGca                                                      >  1:1423770/1‑150 (MQ=255)
                                                                                                 cgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGcac                                                     >  1:1343053/1‑150 (MQ=255)
                                                                                                  gTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGctgt                                                    <  2:668285/150‑4 (MQ=255)
                                                                                                   tGAACGCCCTATCCAGCCTACGTTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAg                                                   <  2:2641019/150‑1 (MQ=255)
                                                                                                      aCGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGGTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGt                                                >  2:2285755/1‑150 (MQ=255)
                                                                                                        gCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCg                                              >  1:1835743/1‑150 (MQ=255)
                                                                                                         cccTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGc                                             >  1:400722/1‑150 (MQ=255)
                                                                                                              tCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAg                                        >  1:114500/1‑150 (MQ=40)
                                                                                                               ccAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAgg                                       <  2:427841/150‑1 (MQ=40)
                                                                                                                 aGCCTACGGGTATGTTCCGGGTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGCTGCGGCGGAAACGCCGTATCCGACCTCGGGGGAGGTGCCGTGTGTAGGCCTGTGCCGGCGCACAGCGTCGCCTCAGGcg                                     >  2:1405781/1‑149 (MQ=11)
                                                                                                                   cctcgggTTTTGTTGCGTTTTGTTGGCCTGATTAGCCGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAAc                                   <  1:2613492/145‑1 (MQ=11)
                                                                                                                   ccTACGTTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAAc                                   <  1:194658/150‑1 (MQ=37)
                                                                                                                   ccTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAAc                                   >  2:933317/1‑150 (MQ=37)
                                                                                                                   ccTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAAc                                   >  1:688520/1‑150 (MQ=37)
                                                                                                                     tACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACgg                                 >  2:1559761/1‑150 (MQ=37)
                                                                                                                     tACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACgg                                 >  1:713184/1‑150 (MQ=37)
                                                                                                                       gggTTTTGTTCGGGTTTGTGGGCGTTATATGACGCACAGCGGCGCATCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTCGGTTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCt                               <  2:1210487/149‑1 (MQ=11)
                                                                                                                       cgtttATGTTCCGGTTTGTAGGCCTTATAAGACGAACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGTTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCt                               <  2:84594/147‑1 (MQ=255)
                                                                                                                       cgtttATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCt                               <  2:840666/147‑1 (MQ=255)
                                                                                                                           tttGTTCGGGTTTGTAGGAGTGATAAGACGCACAGGGTCGAATCAGGCAACGGCTGTCGGAGGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTTGGCCTGATAAGACGCACCGCGTCGCATCTGGCAACGGCTGCCg                           <  2:2192849/148‑1 (MQ=11)
                                                                                                                              gTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCACCGGCTGCCGGAt                        >  2:2048657/1‑150 (MQ=37)
                                                                                                                                tCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgc                      >  1:2399708/1‑150 (MQ=35)
                                                                                                                                  cGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcgg                    >  2:2523871/1‑150 (MQ=33)
                                                                                                                                  cGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcgg                    >  2:810802/1‑150 (MQ=33)
                                                                                                                                    gTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGAGgcggcg                  >  2:2541319/1‑150 (MQ=17)
                                                                                                                                            gCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGcc          >  1:604502/1‑150 (MQ=34)
                                                                                                                                              cTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGa                                                                                                                    >  1:649014/1‑42 (MQ=38)
                                                                                                                                              cTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGa                                                                                                                    >  1:557069/1‑42 (MQ=38)
                                                                                                                                                 aTAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATc     >  2:2311495/1‑150 (MQ=37)
                                                                                                                                                 aTAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATc     >  1:2245318/1‑150 (MQ=37)
                                                                                                                                                   aaGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCg   >  1:1458621/1‑150 (MQ=37)
                                                                                                                                                    aGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCGTATCCGa  >  1:425963/1‑150 (MQ=21)
                                                                                                                                                    |                                                                                                                                                     
CGCCGCTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGA  >  NC_000913/3391990‑3392287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: