Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,522,780 C→G 5.3% intergenic (‑29/‑91) hofM ← / → mrcA DNA catabolic putative pilus assembly protein/penicillin‑binding protein 1a, murein transglycosylase and transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,522,7800CG 5.3% 484.2 / 15.3 133intergenic (‑29/‑91)hofM/mrcADNA catabolic putative pilus assembly protein/penicillin‑binding protein 1a, murein transglycosylase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base C (67/59);  new base G (3/4);  total (70/63)
Fisher's exact test for biased strand distribution p-value = 7.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 137 total aligned reads displayed.

CCAGCGGCAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTCTGCTGGGAGCAGG  >  NC_000913/3522634‑3522929
                                                                                                                                                  |                                                                                                                                                     
ccAGCGGCAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGa                                                                                                                                                    <  1:682329/150‑1 (MQ=255)
 cAGCGGCAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGAc                                                                                                                                                   >  1:2241117/1‑150 (MQ=255)
     ggCAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCAGAGTGACGGGt                                                                                                                                               <  1:282706/150‑1 (MQ=255)
     ggCAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCAGAGTGACGGGt                                                                                                                                               <  1:282699/150‑1 (MQ=255)
     ggCAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGt                                                                                                                                               <  2:2148224/150‑1 (MQ=255)
     ggCAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGt                                                                                                                                               <  1:936462/150‑1 (MQ=255)
      gCAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGta                                                                                                                                              >  1:2585829/1‑150 (MQ=255)
      gCAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGta                                                                                                                                              >  1:793965/1‑150 (MQ=255)
       cAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGtat                                                                                                                                             <  1:2310578/150‑1 (MQ=255)
       cAACCGCCACCAGCGTTGCAAAAAGCATTCTTGTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCACTTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGtat                                                                                                                                             <  1:410683/150‑1 (MQ=255)
          ccGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCAGAGTGACGGGtgataa                                                                                                                                          <  1:977788/150‑6 (MQ=255)
           cGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCa                                                                                                                                         >  1:175561/1‑150 (MQ=255)
             ccaccaGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGTCGGGTATATCAAt                                                                                                                                       >  2:1173162/1‑150 (MQ=255)
                   gCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCt                                                                                                                                 <  2:2319706/150‑1 (MQ=255)
                     gTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTgg                                                                                                                               <  1:1179989/150‑1 (MQ=255)
                        gCAAAAAGCATTCATTTGCGCCCCGCACGATCTCAACCTCAACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTAAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCtt                                                                                                                            <  1:1322750/150‑1 (MQ=255)
                          aaaaaGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGc                                                                                                                          >  1:2069833/1‑150 (MQ=255)
                           aaaaGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGcc                                                                                                                         <  1:1694871/150‑1 (MQ=255)
                           aaaaGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGcc                                                                                                                         <  1:914614/150‑1 (MQ=255)
                           aaaaGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGcc                                                                                                                         <  1:914833/150‑1 (MQ=255)
                            aaaGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCt                                                                                                                        <  1:2382581/150‑1 (MQ=255)
                              aGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCttt                                                                                                                      >  1:212189/1‑150 (MQ=255)
                               gCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTta                                                                                                                     <  1:252216/150‑1 (MQ=255)
                                cATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAAATAGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTtat                                                                                                                    <  2:1266513/150‑1 (MQ=255)
                                          cgcCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACcgcgc                                                                                                          >  2:1036361/1‑150 (MQ=255)
                                             cccGCACGCTCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCgtt                                                                                                       <  2:803533/150‑1 (MQ=255)
                                             cccGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCgtt                                                                                                       >  1:2296937/1‑150 (MQ=255)
                                             cccGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCgtt                                                                                                       >  1:2132846/1‑150 (MQ=255)
                                              ccGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATCCTACcgcgcgggt                                                                                                      >  2:1220113/1‑147 (MQ=255)
                                              ccGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTCCCGCGCgttt                                                                                                      >  2:1931370/1‑150 (MQ=255)
                                              ccGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCgttt                                                                                                      <  2:2185989/150‑1 (MQ=255)
                                               cGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTCCCGCGCgtttg                                                                                                     >  2:1237565/1‑150 (MQ=255)
                                                 cACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCgtttgtt                                                                                                   <  2:1982702/150‑1 (MQ=255)
                                                   cGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTta                                                                                                 <  1:1013406/150‑1 (MQ=255)
                                                    gATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTtat                                                                                                >  1:2206746/1‑150 (MQ=255)
                                                             cgctaccgctTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGccc                                                                                       <  2:1984987/150‑1 (MQ=255)
                                                                 accgctTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTACATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAAt                                                                                   <  2:1018405/150‑1 (MQ=255)
                                                                 accgctTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAAt                                                                                   >  1:2618091/1‑150 (MQ=255)
                                                                 accgctTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAAt                                                                                   <  2:2382444/150‑1 (MQ=255)
                                                                  ccgctTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATg                                                                                  >  1:1395855/1‑150 (MQ=255)
                                                                  ccgctTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATg                                                                                  >  1:536328/1‑150 (MQ=255)
                                                                    gctTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGaa                                                                                >  1:2257464/1‑150 (MQ=255)
                                                                    gctTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGaa                                                                                <  2:1492619/150‑1 (MQ=255)
                                                                     cttcttGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGaaa                                                                               <  1:1015937/150‑1 (MQ=255)
                                                                          tGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTaaa                                                                          <  2:2585829/150‑1 (MQ=255)
                                                                           gCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAAt                                                                         >  1:755111/1‑150 (MQ=255)
                                                                               ttgtAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGa                                                                     >  1:2210561/1‑150 (MQ=255)
                                                                                      tGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTcc                                                              <  2:1873720/150‑1 (MQ=255)
                                                                                      tGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTcc                                                              <  2:331687/150‑1 (MQ=255)
                                                                                       gCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCa                                                             <  1:2717888/150‑1 (MQ=255)
                                                                                       gCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCa                                                             >  2:468381/1‑150 (MQ=255)
                                                                                       gCAAACCAATGTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCa                                                             <  2:1942199/150‑1 (MQ=255)
                                                                                          aaCCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTg                                                          >  1:279006/1‑150 (MQ=255)
                                                                                          aaCCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTg                                                          >  2:1387877/1‑150 (MQ=255)
                                                                                           aCCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGa                                                         >  1:210092/1‑150 (MQ=255)
                                                                                           aCCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGa                                                         >  1:11882/1‑150 (MQ=255)
                                                                                            ccAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGaa                                                        <  2:1280544/150‑1 (MQ=255)
                                                                                                tttGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTc                                                    <  1:1569012/150‑1 (MQ=255)
                                                                                                tttGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTc                                                    <  1:2283099/150‑1 (MQ=255)
                                                                                                   gCCAGATCTTAAATGCCATTGTTATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGATTGCCTTTATACTACCGCGCGTTTGTTTATAAACTTCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTa                                                 <  2:1230053/150‑1 (MQ=255)
                                                                                                   gCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTa                                                 <  2:1230083/150‑1 (MQ=255)
                                                                                                    ccAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTaa                                                >  1:2410920/1‑150 (MQ=255)
                                                                                                     cAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTaaa                                               >  2:2700171/1‑150 (MQ=255)
                                                                                                     cAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTaaa                                               >  2:1525670/1‑150 (MQ=255)
                                                                                                     cAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTaaa                                               >  1:903715/1‑150 (MQ=255)
                                                                                                     cAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTaaa                                               >  1:903763/1‑150 (MQ=255)
                                                                                                     cAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTaaa                                               >  1:2396804/1‑150 (MQ=255)
                                                                                                       gATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGt                                             >  2:2074105/1‑150 (MQ=255)
                                                                                                         tCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTAt                                           >  1:2361081/1‑150 (MQ=255)
                                                                                                           ttAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGAGAAGTTCGTAAAGTAttt                                         >  1:2086260/1‑150 (MQ=255)
                                                                                                            tAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTAtttt                                        <  1:998183/150‑1 (MQ=255)
                                                                                                            tAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTAtttt                                        <  2:2309109/150‑1 (MQ=255)
                                                                                                              aaTGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTAtttttt                                      >  2:524541/1‑150 (MQ=255)
                                                                                                              aaTGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTAtttttt                                      >  1:1143630/1‑150 (MQ=255)
                                                                                                                tGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGa                                    <  1:382588/150‑1 (MQ=255)
                                                                                                                   cATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTGTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATcc                                 >  1:1286176/1‑150 (MQ=255)
                                                                                                                     ttGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCtt                               <  2:274830/150‑1 (MQ=255)
                                                                                                                     ttGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCtt                               <  2:274683/150‑1 (MQ=255)
                                                                                                                      tgtgATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTACCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTg                              <  2:78664/150‑1 (MQ=255)
                                                                                                                           tgatCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTc                         <  1:1036361/150‑1 (MQ=255)
                                                                                                                            gatCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCt                        >  1:1332321/1‑150 (MQ=255)
                                                                                                                               ctcCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGtt                     <  2:2724763/150‑1 (MQ=255)
                                                                                                                                tcCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTg                    >  1:2140086/1‑150 (MQ=255)
                                                                                                                                tcCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTg                    >  1:2139897/1‑150 (MQ=255)
                                                                                                                                 ccTTATCACCCGTCACTCTGACGGGTCTATCAATGCGTCTGGCTTGCCTTTATACTCCCGCGCGTTTGTTTATAAACTGCCCAAATGAACCTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGc                   >  2:33882/1‑150 (MQ=255)
                                                                                                                                 ccTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGc                   <  2:787571/150‑1 (MQ=255)
                                                                                                                                  cTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCa                  >  2:979705/1‑150 (MQ=255)
                                                                                                                                  cTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCa                  <  1:38991/150‑1 (MQ=255)
                                                                                                                                  cTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCa                  <  2:1548704/150‑1 (MQ=255)
                                                                                                                                        aCCCGTCAGAGTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTctgc            >  1:205047/1‑150 (MQ=255)
                                                                                                                                        aCCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTctgc            >  1:1808019/1‑150 (MQ=255)
                                                                                                                                         cccGTCAGAGTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTctgct           >  2:1244105/1‑150 (MQ=255)
                                                                                                                                          ccGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTctgctg          <  1:672465/150‑1 (MQ=255)
                                                                                                                                            gTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTCTGCTggg        >  2:2670498/1‑150 (MQ=255)
                                                                                                                                            gTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTCTGCTggg        >  2:2078273/1‑150 (MQ=255)
                                                                                                                                            gTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTCTGCTggg        >  1:2324778/1‑150 (MQ=255)
                                                                                                                                            gTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTCTGCTggg        >  2:59186/1‑150 (MQ=255)
                                                                                                                                            gTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTCTGCTggg        <  2:773826/150‑1 (MQ=255)
                                                                                                                                             tCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTCTGCTGGGa       <  1:473299/150‑1 (MQ=255)
                                                                                                                                                 tctGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTCTGCTGGGAGCAg   <  2:279006/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CCAGCGGCAACCGCCACCAGCGTTGCAAAAAGCATTCTTTTGCGCCCCGCACGATCGCAACCGCTACCGCTTCTTGCTGTTGTAAATGCAAACCAATTTGCCAGATCTTAAATGCCATTGTGATGATCTCCTTATCACCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGAAACTAAATGGGAAATTTCCAGTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTCTGCTGGGAGCAGG  >  NC_000913/3522634‑3522929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: