| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 126,093 | T→C | 8.4% | A133A (GCT→GCC) | aceF → | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 126,093 | 0 | T | C | 8.4% | 185.5 / 2.6 | 70 | A133A (GCT→GCC) | aceF | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Reads supporting (aligned to +/- strand): ref base T (35/28); new base C (2/4); total (37/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGT > NC_000913/125945‑126237 | cACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTg < 1:479325/150‑1 (MQ=255) ccTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGaa > 1:585596/1‑150 (MQ=255) cAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCg > 2:1290355/1‑150 (MQ=255) cAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCg < 2:1180353/150‑1 (MQ=255) gaagagaagaAAGAAGCAGCTCCGGCAGCAGCCCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACCGTCGCTGagc > 2:851598/1‑148 (MQ=255) gaagagaagaAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTGCCTGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGATGAACAGTCGCTGCTc > 2:809930/1‑150 (MQ=255) aagagaagaAAGAAGCAGCTCCGGCAGCTGCTCCAGCGGCTGCGGCGTCAAATGAGGTTAACTTTCCGGATATCGGCTGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCa < 1:445517/150‑1 (MQ=255) gaagaaAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGt < 1:230123/150‑1 (MQ=255) aataaagaaGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTTGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTa < 2:746539/147‑1 (MQ=255) aagaaagaAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTa < 2:1085930/150‑1 (MQ=255) gaaagaaGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGa > 2:766150/1‑150 (MQ=255) gaaagaaGCAGCTCCGGCAGAAGCACCAGCGGCTGCGGCGGCAAAAGACTTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGAACGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCCCCGTAGa > 2:20121/1‑150 (MQ=255) aagaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg < 2:595670/150‑1 (MQ=255) cagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACa < 2:533745/150‑1 (MQ=255) cagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACa < 1:179777/150‑1 (MQ=255) ggCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTc > 2:9826/1‑150 (MQ=255) cagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCGGCGACGAAGTTGAAGTGACCGAAGTCCTGGTGAAAGTGGGCGAGAAAGTTGCCGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATgg > 2:539517/1‑150 (MQ=17) agcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGAGACGAAGTTGAAGTGACAGAAATGATGGTGAAAGTTGGAGAAAAAGTTGCAGCTGAACAGTCACTGATCACCTTAGAAGCCGAAAAAGCTTCTATGGa > 2:1117064/1‑150 (MQ=14) gcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGaa > 1:772281/1‑150 (MQ=255) caCCAGCGGCGGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGATATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAg < 2:34467/150‑1 (MQ=255) cAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTc < 2:1241558/150‑1 (MQ=255) ggCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGc < 2:585596/150‑1 (MQ=255) ggCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGCTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGc > 2:885410/1‑150 (MQ=255) gcggcgGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAATTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCGCCg > 1:523074/1‑150 (MQ=255) cggcggCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGt < 2:1300589/150‑1 (MQ=255) aGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAc < 1:520130/150‑1 (MQ=255) gACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAcc > 1:790259/1‑150 (MQ=255) cGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAATTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGACGCTGATCACCGTAGAAGGCGACAAGCCTTCTATGGAAGTTCCGGCTCCGTTTGCAGGctacgg > 2:1015613/1‑145 (MQ=17) gTTAACGTTCCGGATATCGGCTGCGACTTAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTg < 2:514458/150‑1 (MQ=255) gTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTg > 1:1092180/1‑150 (MQ=255) gTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTg < 2:1144694/150‑1 (MQ=255) aaCGTTCCGGATATCGGCAGCTACGAATTTGAAGTTACCGTAAACCTGGTGTAAGTAGGCGTTAAAGCTGAAGCTGAACAGTCGCTGATCACCGTATAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGaaa < 2:616335/150‑1 (MQ=25) aaCGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGaaa > 2:1261815/1‑150 (MQ=255) ccGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATc > 2:689385/1‑150 (MQ=255) aagttcatCCGCCGCGAAGAAGTTGAATTCACCGACATCCTGGTCAAAGTTGGCGAAAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCCGCTCCGTTTGCTGGCACCGTGAAAGAGATc < 1:531833/144‑1 (MQ=17) tatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGt > 2:855760/1‑150 (MQ=255) tCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGa > 2:623431/1‑150 (MQ=255) tCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGa > 1:1219230/1‑150 (MQ=255) gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCTCCGTTTGCAGGCGACGTGAAAGACCTGAAAGTCAACg > 2:633056/1‑150 (MQ=14) cgCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTTGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTg < 2:668971/149‑1 (MQ=255) aaGTTGAAGTGACCGAAATCCTGGTGATAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCTCAGTAGAAGGAGACAAGGCTTCTATGGAAGTTCCGGCTGCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACa < 1:218764/150‑1 (MQ=25) aaGTTGAAATCACCGAGATCCTGGTTAAAGTGGGCGACAATGTTGAAGCCGAACAGTCGCTGATCACCGTACAAGGCGACAAGGCATCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACa < 1:237862/150‑1 (MQ=17) aGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAg < 2:1134053/134‑1 (MQ=11) aGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAg > 1:1134053/1‑134 (MQ=11) gTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGCCGCTGAACAGTCACTGATCa < 2:865227/64‑1 (MQ=14) gTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCACTGATCa > 1:865227/1‑64 (MQ=255) gAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAATGAGCTGAAAGTGAACGTGGGTGAAAAAgtg > 1:798034/1‑150 (MQ=255) aatGACCGAGATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCCGAACAGTAGCTGATCACCGTAGAAGGCGACAATGATTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTc < 1:882019/148‑1 (MQ=21) gagACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTTAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGATCCGTTTGCTGGCGGCGTTATAGATATCAAATTAAACTTGGGAGAAAAAgtgaca > 2:992254/3‑147 (MQ=18) gTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCAGGCGACGTGAATGAGCTCAAAGTGAACGTGGGAGAGAAAgtgaca > 2:423705/1‑147 (MQ=18) gACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGATCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTAc > 1:1233041/1‑150 (MQ=255) cccGAAATCCTGGTGAAAGTTGGCGAAAAAGTTGAAGCTGAACAGTCGCTGATCACGGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTAcc < 2:457217/149‑1 (MQ=255) cTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTg > 1:796141/1‑150 (MQ=255) ggTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGAt > 2:837824/1‑150 (MQ=255) ggTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGAt > 1:609380/1‑150 (MQ=255) ggTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCgg > 2:354158/1‑80 (MQ=255) ggTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCgg < 1:354158/80‑1 (MQ=255) gAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTAt > 1:1321831/1‑150 (MQ=255) caaaGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAACGCGTCTATGGAAGTTCCGTCTCCGGTTGCTGGAAGCGTGAAAGAGATCAAAGTGAAAGTTGGAGACAAAGAGACTACCGGCTCGCTGATTAt > 2:1092904/2‑150 (MQ=17) aaGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAATGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGa < 2:919100/96‑1 (MQ=11) aaGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGa > 1:919100/1‑96 (MQ=11) aGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCATAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGCCGTGAAAGAGATCAAAGTGAACGTTGGAGAAAAAGTGACTACAGGCTCGCTGATTATGGt > 1:876008/1‑150 (MQ=18) gatGGCGATAAAGCTGAAGCGGAACTGTCTCTGATCACCGTTGCAGGCGACTAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCATATTGAACGTGGGTGACAAAGTGTCTACCGGAACGCTGATTATGGTc < 1:245983/148‑1 (MQ=25) tGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCtt > 2:839301/1‑150 (MQ=255) cGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGa > 1:1264658/1‑150 (MQ=255) aTAAAGTTGAAGCTGATCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAg < 1:1290355/150‑1 (MQ=255) gTTGAAGCTGAACAGTCGCTGATCACCTTAGAACGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTcgcg < 1:779824/150‑1 (MQ=255) ttGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCgg < 1:766150/150‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCggg < 1:719070/150‑1 (MQ=255) gAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCCGATTATGGTCTTCGAAGTCGCGGGt < 1:9826/150‑1 (MQ=255) | CACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGT > NC_000913/125945‑126237 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |