Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,257,229 T→A 7.4% S39T (TCC→ACC)  lexA → transcriptional repressor of SOS regulon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,257,2290TA 7.4% 231.7 / 5.0 79S39T (TCC→ACC) lexAtranscriptional repressor of SOS regulon
Reads supporting (aligned to +/- strand):  ref base T (37/36);  new base A (4/2);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

CACAGCATAACTGTATATACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGCTGCCGGTGAACCACT  >  NC_000913/4257081‑4257377
                                                                                                                                                    |                                                                                                                                                     
cacaGCATAACTGTATATACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGgttccgttc                                                                                                                                                      >  2:1118883/1‑150 (MQ=255)
cacaGCATAACTGTATATACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGgttccgttc                                                                                                                                                      >  1:553050/1‑150 (MQ=255)
  caGCATAACTGTATATACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTccc                                                                                                                                                    <  2:1167856/150‑1 (MQ=255)
    gCATAACTGTATATACACCCAGGGGGCTGAATGAAAGCGTTAACGGCCAGGCAACAAGATGTGTTTGATCTCATCCGTGATAACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCa                                                                                                                                                  <  2:1305395/150‑1 (MQ=255)
    gCATAACTGTATATACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCa                                                                                                                                                  <  2:1310501/150‑1 (MQ=255)
       tAACTGTATATACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAAc                                                                                                                                               >  1:841414/1‑150 (MQ=255)
       aaaCTGTATATACACCCAGGGGGCGTAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGAACGGAACCCCAAAc                                                                                                                                               <  1:387224/149‑1 (MQ=255)
         aCTGTATATACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGATATCGCGCAGCGTTTGGGGTTCCGTTCCCCAACcgc                                                                                                                                             >  1:225466/1‑150 (MQ=255)
         aCTGTATAAACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAAcgc                                                                                                                                             >  2:493705/1‑150 (MQ=255)
           tGTATAAACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGAACGGAACCCCAAAcgctg                                                                                                                                           <  1:866449/150‑3 (MQ=255)
            gTATATACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGc                                                                                                                                          <  1:624053/150‑1 (MQ=255)
               aataCACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTga                                                                                                                                       <  1:929090/149‑1 (MQ=255)
                  acacCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTgaaga                                                                                                                                    >  1:708011/1‑150 (MQ=255)
                              cGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCACACGCGGCTGAAGAACATCTGAAGgc                                                                                                                        >  2:895535/1‑150 (MQ=255)
                                 aaTGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCt                                                                                                                     <  2:722763/150‑1 (MQ=255)
                                   tGAAAGCTTTAACGGCCAGGCTACAAGAGGGGGTTGATCTCATCCGTTATCACAGCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGGTCCGTTCCCCAAACGCGGCTGAAGATCATCTGAAGGCGCTgg                                                                                                                   <  1:708265/150‑1 (MQ=255)
                                       aGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACg                                                                                                               <  2:391133/150‑1 (MQ=255)
                                       aGCGTCAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACg                                                                                                               >  1:692533/1‑150 (MQ=255)
                                        gCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGc                                                                                                              >  1:1095567/1‑150 (MQ=255)
                                          gTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCCAACGCGGCTGAAGACCATCTGAAGGCGCTGGCACGCaa                                                                                                            >  2:967819/1‑150 (MQ=255)
                                               cGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCg                                                                                                       <  1:9818/150‑1 (MQ=255)
                                                 gCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCACACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGtt                                                                                                     >  1:180817/1‑150 (MQ=255)
                                                 gCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACTCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGtt                                                                                                     >  2:994152/1‑150 (MQ=255)
                                                   cAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTAt                                                                                                   >  1:86226/1‑150 (MQ=255)
                                                     ggCAACAAGAGGTGTTTGATCTCATCCGTTATCACATCAGCCAGACAGGAATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCACAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTg                                                                                                 <  1:831142/150‑1 (MQ=255)
                                                     ggCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCAGAAGGCGCTGGCCCGCAAAGGCGTTATTg                                                                                                 >  1:1240248/1‑150 (MQ=255)
                                                      gCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGa                                                                                                >  1:67091/1‑150 (MQ=255)
                                                       caacaaGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGaa                                                                                               >  2:125631/1‑150 (MQ=255)
                                                       caacaaGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCACAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGaa                                                                                               >  2:1051332/1‑150 (MQ=255)
                                                            agagGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGt                                                                                         >  1:417203/1‑150 (MQ=255)
                                                                gtgtTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTcc                                                                                     <  2:553050/150‑1 (MQ=255)
                                                                gtgtTTGATCTCATCCGTGATCAAATCAGCCAGACATGTTAGCCGCCGACGCGTGCTGAAATCGCGCATCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTcc                                                                                     <  2:368162/150‑1 (MQ=255)
                                                                  gtTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCgg                                                                                   >  1:1103061/1‑150 (MQ=255)
                                                                  gtTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCgg                                                                                   >  2:1324156/1‑150 (MQ=255)
                                                                  gtTTGATCTCATCCGTGATCACATCAGCCAAACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCgg                                                                                   <  2:892835/150‑1 (MQ=255)
                                                                   tttGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGgc                                                                                  <  2:1266329/150‑1 (MQ=255)
                                                                   tttGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGgc                                                                                  <  2:121532/150‑1 (MQ=255)
                                                                     tGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTTCCGgcg                                                                                 >  2:65004/1‑150 (MQ=255)
                                                                     tGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGgcgc                                                                                >  2:1123278/1‑150 (MQ=255)
                                                                        tctcATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATc                                                                             <  1:1118883/150‑1 (MQ=255)
                                                                            aTCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCAcgc                                                                         >  2:487170/1‑150 (MQ=255)
                                                                              ccGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCgg                                                                       <  1:497362/150‑1 (MQ=255)
                                                                               cGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCggg                                                                      <  2:52087/150‑1 (MQ=255)
                                                                                     cacaTCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGt                                                                >  1:752780/1‑150 (MQ=255)
                                                                                      acaTCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTc                                                               <  2:1103061/150‑1 (MQ=255)
                                                                                      acaTCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTc                                                               >  2:469802/1‑150 (MQ=255)
                                                                                       caTCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTTTTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCt                                                              >  1:928723/1‑150 (MQ=255)
                                                                                        aTCAGCGAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCAGGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTg                                                             <  1:255218/150‑1 (MQ=255)
                                                                                         tCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGt                                                            <  2:718836/150‑1 (MQ=255)
                                                                                         tCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGt                                                            <  2:441706/150‑1 (MQ=255)
                                                                                           aGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTg                                                          <  2:493862/150‑1 (MQ=255)
                                                                                           aGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTg                                                          >  2:885464/1‑150 (MQ=255)
                                                                                               aGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCagg                                                      <  2:559010/150‑1 (MQ=255)
                                                                                                gACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCagga                                                     >  2:966404/1‑150 (MQ=255)
                                                                                                gACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCagga                                                     >  2:153370/1‑150 (MQ=255)
                                                                                                  cAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCaggaag                                                   >  1:554372/1‑150 (MQ=255)
                                                                                                      tATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCaggaagagga                                               <  1:1254871/150‑1 (MQ=255)
                                                                                                        tGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACTCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCaggaagaggaag                                             <  1:994152/150‑1 (MQ=255)
                                                                                                           cgccgACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACCTCTGAAGGCGCTGGCACGCAAAGGGGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGaagaag                                          >  2:1234257/1‑150 (MQ=255)
                                                                                                                 cgcgTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGc                                    <  1:446120/150‑1 (MQ=255)
                                                                                                                  gcgTGCGGAAATCGCGCGGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGcc                                   >  1:1253791/1‑150 (MQ=255)
                                                                                                                   cgTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCg                                  >  1:564307/1‑150 (MQ=255)
                                                                                                                   cgTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCg                                  >  2:61000/1‑150 (MQ=255)
                                                                                                                       cGGAAATCGCTCAGCGTTTGGGTTTCCGTTCACCAAACGCGGCTGAAGAACATCTGAAGGCGCTTGCACGCAAAGGCGTTATTGAAATTGTTTCCTGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGATGAAGAAGGGTTGCAGCTgg                              <  2:1010934/150‑1 (MQ=255)
                                                                                                                         gAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCGGAAGAACAGCTGAAGGCGCTGGCGCGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATGCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTa                            >  1:175608/1‑150 (MQ=255)
                                                                                                                            aTCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGt                         <  1:125173/150‑1 (MQ=255)
                                                                                                                            aTCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGt                         <  1:889409/150‑1 (MQ=255)
                                                                                                                              cgcgcgGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCg                       <  2:1253791/150‑1 (MQ=255)
                                                                                                                               gcgcAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCGGAAGAACAGCTGAAGGTGCTGGCACGCAAAGGCGGTATTGAAATTGTTTCCGGCGCATCACGCGGGAGTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGGAGGTCgt                      >  1:121660/1‑150 (MQ=255)
                                                                                                                                agccgCGTTTGGGGAACGGAACCCCAAACGCTGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCgtg                     >  1:339680/5‑150 (MQ=255)
                                                                                                                                 gccgCGTTTGGGGAACGGAACCCCAAACGCTGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCgtgt                    >  2:22645/4‑150 (MQ=255)
                                                                                                                                 gccgCGTTTGGGGAACGGAACCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCgtgt                    >  2:404515/4‑150 (MQ=255)
                                                                                                                                   aGCGGTTGGGGGTCCGTTCCCCAATCGGGGCGGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTCTTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTgg                  <  1:126021/150‑1 (MQ=255)
                                                                                                                                    gCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGc                 <  2:374766/150‑1 (MQ=255)
                                                                                                                                     cGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGCt                <  2:708011/150‑1 (MQ=255)
                                                                                                                                     cGTTTGGGGAACGGAACCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGCt                >  1:1343367/1‑150 (MQ=255)
                                                                                                                                       tttGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGCTGc              <  2:180817/150‑1 (MQ=255)
                                                                                                                                        ttGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGCTGcc             <  2:1341728/150‑1 (MQ=255)
                                                                                                                                         tGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAATTTGTTTCCGGCGCATCACGCGGGCTTCGTCTGTTGCAGGAAGAGGAAGAAGTGTTGCCGCTGGTAGGTCGTGTGGCTGCCg            >  2:643386/1‑150 (MQ=255)
                                                                                                                                         tGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGCTGCCg            >  1:1282313/1‑150 (MQ=255)
                                                                                                                                           gggTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGCTTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGCTGCCGGt          >  1:375534/1‑150 (MQ=255)
                                                                                                                                             gttccgttacCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGACGGGTTGCCGCTGGTAGGTCGTGTGGCTGCCGGTGa        >  1:190543/1‑150 (MQ=255)
                                                                                                                                                ccgttccCCATACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTTTTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGCTGCCGGTGAAcc     <  2:1264735/150‑1 (MQ=255)
                                                                                                                                                ccgttccCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGCTGCCGGTGAAcc     <  2:67091/150‑1 (MQ=255)
                                                                                                                                                   ttccCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGCTGCCGGTGAACCACt  <  1:451576/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CACAGCATAACTGTATATACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGCTGCCGGTGAACCACT  >  NC_000913/4257081‑4257377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: