Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	469,744	C→T	6.4%	L292L (CTG→TTG)	mdlA →	putative multidrug ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	469,744	0	C	T	6.4%	189.2 / 2.6	62	L292L (CTG→TTG)	mdlA	putative multidrug ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (33/25);  new base T (2/2);  total (35/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

CTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAG  >  NC_000913/469595‑469893                                                                                                                                                      |                                                                                                                                                      cTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGACAATgc                                                                                                                                                       >  1:1086411/1‑150 (MQ=255)                 cATCTTTATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGAt                                                                                                                                       <  1:145913/150‑1 (MQ=255)                      atatCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTa                                                                                                                                  <  2:798289/150‑1 (MQ=255)                        atCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGATTAACGCTGGGCCAGCTCACCAGTTTTAAGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTaaa                                                                                                                                >  1:492971/1‑149 (MQ=255)                        atCGCGATTGGTAGGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAAc                                                                                                                                <  2:157602/150‑1 (MQ=255)                                 ggTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGaa                                                                                                                       >  1:686523/1‑150 (MQ=255)                                  gTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAAc                                                                                                                      >  2:908411/1‑150 (MQ=255)                                  gTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAAc                                                                                                                      <  1:451965/150‑1 (MQ=255)                                     tGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTg                                                                                                                   <  2:401027/150‑1 (MQ=255)                                      ggCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTgg                                                                                                                  <  1:1199882/150‑1 (MQ=255)                                         gAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTGTAACATTGTGGAACGTGGTAg                                                                                                               >  2:759052/1‑150 (MQ=255)                                                    cGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACa                                                                                                    >  1:337608/1‑150 (MQ=255)                                                     gATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAg                                                                                                   >  2:395866/1‑150 (MQ=255)                                                       tgggctgcGGTAGGTGGATGCTGGTGGAGGGCAGTTTATCGCTGGGCCAGCTCACCAGTTTTATGATGTATTTACGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGcc                                                                                                 <  2:1204877/148‑1 (MQ=255)                                                        tGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCg                                                                                                <  2:582427/150‑1 (MQ=255)                                                         ggcggcGGTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCCCCAGTTTTATGATGTATTTAGGGCTGATGATATGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTACTGCTGCGTACAGCCGt                                                                                               >  2:192736/1‑150 (MQ=255)                                                           cggcggTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCCCCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGTTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCCTACAGCCGTAt                                                                                             >  2:722546/1‑150 (MQ=255)                                                           cggcggTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGTTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTAt                                                                                             >  2:1071019/1‑150 (MQ=255)                                                              cggTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTcg                                                                                          <  2:44144/150‑1 (MQ=255)                                                                gTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTcgcg                                                                                        <  1:1204348/150‑1 (MQ=255)                                                                    ttGGATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGAt                                                                                    <  2:1046128/150‑1 (MQ=255)                                                                      ggATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCGGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGc                                                                                  <  1:1096366/150‑1 (MQ=255)                                                                       gATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCCCCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCt                                                                                 >  2:1321835/1‑150 (MQ=255)                                                                           gtggtgCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCg                                                                             >  2:1271866/1‑150 (MQ=255)                                                                               tgCAGGGCAGTTTAACGCTGGGCCAGCTCACAAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGatg                                                                         <  1:1182200/150‑3 (MQ=255)                                                                                gCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACCGCCGTATTCGCGCGATGCTGGCGGAAgc                                                                        >  1:370162/1‑150 (MQ=255)                                                                                   gggCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGcc                                                                     >  2:1157563/1‑150 (MQ=255)                                                                                        tgttAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGTATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCggtgg                                                                <  1:674365/148‑1 (MQ=255)                                                                                        gTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCggtgg                                                                >  1:1083635/1‑150 (MQ=255)                                                                                        gTTTAACGCTGGGCCAGCTCACCAGTATTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCggtgg                                                                >  2:652250/1‑150 (MQ=255)                                                                                        gTTTAACCCTGGGCCAGCTCACCAGATTTATGATGTTTTTAGGTCTGATGATTTGGCCAATTCTGGCGCTGGCATGGATGTTAAACATTGTGGATCGTGGTAGTGCTTCGTACAGCCGTATTCGCGAGATTCTGGCGGAAGCGCCggtgg                                                                >  2:88992/1‑150 (MQ=255)                                                                                             aCGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGAGGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAAc                                                           <  1:831495/150‑1 (MQ=255)                                                                                                 cgggCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATg                                                       <  1:765629/149‑1 (MQ=255)                                                                                                   ggCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGTAACGTGGGAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGt                                                     >  1:263177/1‑150 (MQ=255)                                                                                                   ggCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGt                                                     >  1:157357/1‑150 (MQ=255)                                                                                                    gCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTa                                                    >  1:1332921/1‑150 (MQ=255)                                                                                                           cACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAAcc                                             >  2:723118/1‑150 (MQ=255)                                                                                                           cACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAAcc                                             <  2:337608/150‑1 (MQ=255)                                                                                                                 ttttATGATGTATTTAGGGCTGATGGTTTGGCCAATGCTGGCGCTGGCATGGATTTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGcc                                       <  2:913552/150‑1 (MQ=255)                                                                                                                  tttATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCg                                      >  1:428946/1‑150 (MQ=255)                                                                                                                  tttATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCg                                      >  2:677842/1‑150 (MQ=255)                                                                                                                   ttATGATGTATTTAGGTATGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGACGTATTCGCGCGATGCTGGCGGAAGCGCCGGAGGTGAACGATGGTAGCGAACCCGTGCCgg                                     >  2:1081478/1‑150 (MQ=255)                                                                                                                    tATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGa                                    >  1:161172/1‑150 (MQ=255)                                                                                                                      tgatgTATTTAGGTCTGATGATTTGGCCAATGTTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTTTTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGATCGTTGGTAGCGAACCCGTGCCGGAAg                                  <  1:722546/150‑1 (MQ=255)                                                                                                                      tgatgTATTTAGGTCTGATGATTTGGCCAATGTTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAg                                  <  1:1071019/150‑1 (MQ=255)                                                                                                                      tgatgTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAg                                  >  1:831825/1‑150 (MQ=255)                                                                                                                      tgatgTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAg                                  >  2:508862/1‑150 (MQ=255)                                                                                                                      tgatgTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGAGCCGGAAg                                  >  2:1034480/1‑150 (MQ=255)                                                                                                                       gatgTATTTAGGGATGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAgg                                 <  2:236183/150‑1 (MQ=255)                                                                                                                        atgTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAggg                                >  2:1069618/1‑150 (MQ=255)                                                                                                                        atgTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAggg                                >  1:1300061/1‑150 (MQ=255)                                                                                                                         tgTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGc                               >  1:1158245/1‑150 (MQ=255)                                                                                                                           tATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGt                             >  2:1082352/1‑150 (MQ=255)                                                                                                                              ttAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGc                          <  1:970361/150‑1 (MQ=255)                                                                                                                               tAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCg                         <  1:549091/150‑1 (MQ=255)                                                                                                                                  gTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAAc                      <  1:1167817/150‑1 (MQ=255)                                                                                                                                      gatgatTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTggt                  >  1:484134/1‑149 (MQ=255)                                                                                                                                         gatTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGt               <  2:492971/150‑1 (MQ=255)                                                                                                                                              ggCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAata          >  2:1314796/1‑150 (MQ=255)                                                                                                                                              ggCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAata          >  1:1008070/1‑150 (MQ=255)                                                                                                                                                ccAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATAtt        >  1:439948/1‑150 (MQ=255)                                                                                                                                                    tGCTGGCTATGGCATGGATGTTTAACTTTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTTGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCAcc    <  2:924516/150‑1 (MQ=255)                                                                                                                                                    tGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCAcc    <  1:1227237/150‑1 (MQ=255)                                                                                                                                                      ctggcgctggcATGTAGGTTTAACATTGTGGAACGTGGGAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAg  <  1:923667/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                      CTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGCCAGCTCACCAGTTTTATGATGTATTTAGGTCTGATGATTTGGCCAATGCTGGCGCTGGCATGGATGTTTAACATTGTGGAACGTGGTAGTGCTGCGTACAGCCGTATTCGCGCGATGCTGGCGGAAGCGCCGGTGGTGAACGATGGTAGCGAACCCGTGCCGGAAGGGCGTGGCGAACTGGATGTAAATATTCACCAG  >  NC_000913/469595‑469893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑