| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 554,812 | A→G | 6.8% | T68A (ACC→GCC) | cysS → | cysteinyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 554,812 | 0 | A | G | 6.8% | 167.9 / 3.4 | 59 | T68A (ACC→GCC) | cysS | cysteinyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base A (25/30); new base G (2/2); total (27/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
CACGCCGGGGAAGTCGGCATGTACGTGTGTGGAATCACCGTTTACGATCTCTGTCATATCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAAATGCACAAAGATTTTGATGCTTTGAACATTCTGCGCCCGGATATGGAGCCGCGCGCGACGCACC > NC_000913/554665‑554959 | cACGCCGGGGAAGTCGGCTTGTACGTGTGTGGAATCACCGTTTACGATCTCTGTCATATCGGTCACGGGCGTACCGTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTAcc > 1:78721/1‑150 (MQ=255)cACGCCGGGGAAGTCGGCATGTACGTGTGTGGAATCACCGTTTACGATCTCTGTCATATCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTAcc > 1:125466/1‑150 (MQ=255) ccGGGGAAGTCGGCATGTACGTGTGTGGAATCACCGTTAACGATCTCTGGCATATCGGGCACGGGCGTACCTTTGTTGCGTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGata < 2:807440/150‑1 (MQ=255) ggtgAAGTCGGCATGTACGTGTGTGGAATCACCGTTTACGATCTCTGTCATATCCGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTATCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATc < 2:554126/147‑1 (MQ=255) aaGTCGGCATGTACGTGTGTGGAATCACCGTTTACGATCTCTGTCATATCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATcgacg < 1:859066/150‑1 (MQ=255) gTCGGCATGTACGTGTGTGGAATCACCGTTTACGATCTCTGTCATATCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCgacgac > 1:571119/1‑150 (MQ=255) cGGCATGTACGTGTGTGGAATCACCGTTTACGATCTCTGTCATATCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACaa > 2:796212/1‑150 (MQ=255) ggCATGTACGTGTGTGGAATCACCGTTTACGATCTCTGTCATATCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACaaa > 1:336117/1‑150 (MQ=255) aTGTACGTGTGTGGAATCACCGTTTACGATCTCTGTCATATCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTAACGATATCGACGACAAAatc > 1:327205/1‑150 (MQ=255) aCGTGTGTGGAATGAACGTTTACGATCTCTGTCATATCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGTTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAatcatga < 1:716553/150‑3 (MQ=255) gtgGAATCACCGTTTACGATCTCTGTCATATCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAAcgcg > 1:35553/1‑150 (MQ=255) gggAATCACCGTTTACGATCTCTGTCATATCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACgcgc < 1:993427/149‑1 (MQ=255) ggAATCACCGTTTACGATCTCTGTCATATCGGTCACGGGCGTACCGTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGcc < 2:78721/150‑1 (MQ=255) gTTTACGATCTCTGTCATATCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAAAGCGCCAATGAAAAt > 2:347064/1‑150 (MQ=255) gATCTCTGTCATATCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATGACCGATATCGACGACAAAATCATCAAACGAGCCAATGAAAATGGCGaa > 1:776304/1‑150 (MQ=255) aTCTCTGTCATATCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGaaa > 1:334338/1‑150 (MQ=255) ctGTCATATCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCtt < 2:1105110/150‑1 (MQ=255) gTCATATCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTtg > 1:967637/1‑150 (MQ=255) tCATATCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTtgt < 1:281624/150‑1 (MQ=255) tCATATCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTtgt < 1:626410/150‑1 (MQ=255) tCATATCGGTCACGGGCGTACCATTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCTGCTATAAACTGAAGTATGAGCGCAACATTACCGATATCGACGACAAACTCATCAAACGCGCCAATGAAAATGGCGAAAGCTTtgt < 1:164132/150‑1 (MQ=255) cATATCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACCGAAGTATGTGCGCAACATTACCGAAATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGCAAGCTTtgtg > 2:609474/1‑150 (MQ=255) atatCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTgg < 2:1344071/150‑1 (MQ=255) atatCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTgg < 1:194990/150‑1 (MQ=255) atatCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTgg < 2:1133362/150‑1 (MQ=255) tatCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCTATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGc < 2:1208008/150‑1 (MQ=255) cGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATTAACTGAAGTATGTGCGCAACATTACCGATATCGACGCCAAAATCATCAAACGCGCCAATGAAAATGGCGATAGCTTTGTGGCGAt > 1:1163134/1‑150 (MQ=255) ggTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAGCATTGCCGATATCGACGACTAAAGCATCAAACGCGCCAAGGAAAAGGTCGAAAGCTTTGTGGCGAtg > 2:103223/1‑150 (MQ=255) ggTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTGCCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGAtg > 2:1275382/1‑150 (MQ=255) gTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCTGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACTCGCCAATGAAAATGGCGAAAGCTTTGTGGCGAtgg < 1:819844/150‑1 (MQ=255) gTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGTCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGAtgg < 2:984390/150‑1 (MQ=255) gTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGAtgg > 1:1288081/1‑150 (MQ=255) aCGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTACGCAACATTACCGATATCGACGACAAAATCATCAACCGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGAtggtgg < 1:801257/150‑1 (MQ=255) tACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCAt > 2:711189/1‑150 (MQ=255) aCCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATg < 2:183627/150‑1 (MQ=255) gttgCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGcc < 2:728799/150‑1 (MQ=255) gttgCAGTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGcc < 1:202121/150‑1 (MQ=255) ttGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAAATGc < 1:419452/150‑1 (MQ=255) gACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAAATGcac < 1:1265599/150‑1 (MQ=255) aCGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAATTGcaca > 2:996374/1‑150 (MQ=255) cgcgcTATCTGCGTTTCCTCGTCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAAATGCACAAAGATTTTg < 2:742339/150‑1 (MQ=255) cgcgcTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAAATGCACAAAGACTTTg > 2:1067751/1‑150 (MQ=255) tATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCACCATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAAATGCACAAAGATTTTGATGCt > 1:893216/1‑150 (MQ=255) tCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAAATGCACAAAGATTTTGATGCttt > 1:275421/1‑150 (MQ=255) cGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAACCGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAAATGCACAAAGATTTTGATGCTTTGaaa > 2:1281472/1‑149 (MQ=255) tCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCAGCAAACGCGCCAATGAAAATGGCGATAGCGTTGTGGAGATGGTGGATCGCATGATCGCCGAAATGCACAAAGATGTTTATGCTTTGAACAtag > 2:262639/1‑148 (MQ=255) ccTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAAATGCACAAAGATTTTGATGCTTTGAACATTCt < 2:571119/150‑1 (MQ=255) cGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGAGGGTGGATCGCATGATCGCCGAAATGCACAAAGATTTTGATGCTTTGAACATTCTgcg < 1:837448/150‑1 (MQ=255) aaaCTGTAGTATGTGCGCAACATTACCGATATCGACGTCAAAATCATCAAACGCGCCAATCATAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCTCCGAAATGCACAAAGAGTTTGATGCTTTGAACATTCTGCGCCCGGat < 2:275572/150‑1 (MQ=255) cTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAACCGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAAATGCACAAAGATTTTGATGCTTTGAACATTCTGCGCCCGGATATg < 1:1183237/150‑1 (MQ=255) aGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAAATGCACAAAGATTTTGATGCTTTGAACATTCTGCGCCCGGATATGGAGc > 2:28638/1‑150 (MQ=255) gTATGTGCGCAACATTACCGATATCGACGACAAAATCCTCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAAATGCACAAAGATTTTGATGCTTTGAACATTCTGCGCCCGGATATGGAGcc < 2:846520/150‑1 (MQ=255) gcgcAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAAATGCACAAAGATTTTGATGCTTTGAACATTCTGCGCCCGGATATGGAGCcgcgcgc > 2:417666/1‑150 (MQ=255) gcAACATTGCCGATATCGACGACAAAATCATCTCACGCGCCACTGAAATAGGCGAAAGCTTTGAGGCGATGGAGGATCGCAGGATCGCCGAAATGCACAAAGATTTTGATGCTTTGAAGATTCTGCGCCCGGATATGGAGCCGCGCGCGa < 1:103223/150‑1 (MQ=255) gcAACATTGCCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAAATGCACAAAGATTTTGATGCTTTGAACATTCTGCGCCCGGATATGGAGCCGCGCGCGa < 1:1275382/150‑1 (MQ=255) aaCATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAAATGCACAAAGATTTTGATGCTTTGAACATTCTGCGCCCGGATATGGAGCCGCGCGCGACg > 2:604766/1‑150 (MQ=255) aTTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAAATGCACAAAGATTTTGATGCTTTGAACATTCTGCGCCCGGATATGGAGCCGCGCGCGACGCAc < 2:1231306/150‑1 (MQ=255) aTTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAAATGCACAAAGATTTTGATGCTTTGAACATTCTGCGCCCGGATATGGAGCCGCGCGCGACGCAc > 1:775204/1‑150 (MQ=255) ttACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAAATGCACAAAGATTTTGATGCTTTGAACATTCTGCGCCCGGATATGGAGCCGCGCGCGACGCAcc < 2:1288081/150‑1 (MQ=255) | CACGCCGGGGAAGTCGGCATGTACGTGTGTGGAATCACCGTTTACGATCTCTGTCATATCGGTCACGGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAACTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAAAATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAAATGCACAAAGATTTTGATGCTTTGAACATTCTGCGCCCGGATATGGAGCCGCGCGCGACGCACC > NC_000913/554665‑554959 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |