Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,226,835 C→A 5.2% T79K (ACA→AAA)  ycgJ → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,226,8350CA 5.2% 243.6 / 2.6 89T79K (ACA→AAA) ycgJuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (42/42);  new base A (2/3);  total (44/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

CTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTA  >  NC_000913/1226687‑1226984
                                                                                                                                                    |                                                                                                                                                     
cTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGTAAATATCTCGGCGAAAAAGCCGCTGAAAACTAACAATCATTACAAGGCAACGATCCCAGCGAATTTACa                                                                                                                                                      >  2:153433/1‑150 (MQ=255)
cTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACa                                                                                                                                                      >  1:1165363/1‑150 (MQ=255)
   cAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATGTAAATTc                                                                                                                                                   <  2:587677/150‑1 (MQ=255)
     aTCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGc                                                                                                                                                 <  2:980868/150‑1 (MQ=255)
         ccGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATGTAAATTCGCtggg                                                                                                                                             <  1:97781/150‑4 (MQ=255)
           ggCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACgg                                                                                                                                           <  2:311189/150‑1 (MQ=255)
            gCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGgt                                                                                                                                          >  2:424596/1‑150 (MQ=255)
                  ggcgTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTAttt                                                                                                                                    >  1:986846/1‑150 (MQ=255)
                      ttgttTGCGAGCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAATCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACAATCGCTAACATTGTATTTTgag                                                                                                                                >  2:669944/1‑148 (MQ=255)
                        gttTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGAt                                                                                                                              <  2:986850/150‑1 (MQ=255)
                         tttGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGACTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATg                                                                                                                             <  1:692347/150‑1 (MQ=255)
                          ttGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATATTCCGATGt                                                                                                                            >  1:1099935/1‑150 (MQ=255)
                           tGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTGCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTTCAAGGCTACGATCCCAGCGAATTTACATTCGCTATCGGTGTATTTTGCTATGtt                                                                                                                           <  2:608654/150‑1 (MQ=255)
                             cGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTaa                                                                                                                         >  1:718386/1‑150 (MQ=255)
                             cGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTaa                                                                                                                         >  1:782399/1‑150 (MQ=255)
                               aCCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAg                                                                                                                       <  1:902204/150‑1 (MQ=255)
                                cccGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGa                                                                                                                      <  1:863168/150‑1 (MQ=255)
                                 ccGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGaa                                                                                                                     <  1:503277/150‑1 (MQ=255)
                                  cGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTaa                                                                                                                         >  2:844547/1‑145 (MQ=255)
                                  cGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTaa                                                                                                                         <  1:844547/145‑1 (MQ=255)
                                  cGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGata                                                                                                                    >  2:1272397/1‑148 (MQ=255)
                                    tataTTTGTGTGAACTCAGATGGCATGTCCCCAGAGTTAACAAGGAAATATCTCGTAGAAAAAGCAGCTGAAAACTTACAATAAGTACCAGGCTACGATCCCAGCGAATTTACATTAGCTAACGGTGTATTATGGAATGTTTAAGaaaaa                                                                                                                  >  2:58812/1‑150 (MQ=255)
                                      tatTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAAtt                                                                                                                >  1:1250333/1‑150 (MQ=255)
                                      tatTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAAtt                                                                                                                <  2:1280460/150‑1 (MQ=255)
                                      tatTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGAGGGTAAAGAAAAAtt                                                                                                                >  2:517676/1‑150 (MQ=255)
                                        tttGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTAt                                                                                                              >  1:1257181/1‑150 (MQ=255)
                                               gAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGtga                                                                                                       >  2:983618/1‑150 (MQ=255)
                                                aaCTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGtgat                                                                                                      >  2:987618/1‑150 (MQ=255)
                                                 aCTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGtgatg                                                                                                     >  2:1015432/1‑150 (MQ=255)
                                                 aCTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATACGCTAACAGTGTATTATGCGATGTTAAAGAAAAATTATGTCGtcatg                                                                                                     >  1:251388/1‑150 (MQ=255)
                                                  cTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGtgatga                                                                                                    >  2:1269949/1‑150 (MQ=255)
                                                        tGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTa                                                                                              >  1:356707/1‑150 (MQ=255)
                                                            aTTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAATGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTAtttt                                                                                          <  2:826680/150‑1 (MQ=255)
                                                             tttCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATAACAAGGCTACCATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTg                                                                                         <  2:1248103/150‑1 (MQ=255)
                                                              ttCCCCAGAGTTAACAACGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTgg                                                                                        <  2:567402/150‑1 (MQ=255)
                                                                ccccAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGgtg                                                                                      <  2:1237540/150‑1 (MQ=255)
                                                                ccccAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGgtg                                                                                      >  2:939619/1‑150 (MQ=255)
                                                                ccccAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGgtg                                                                                      <  1:944155/150‑1 (MQ=255)
                                                                 cccAGAGTTAACAACGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCAATACAACGCTACGATCCCACCGAATTTACATTCGCTAACGGTGTATTTTACCATGTTAAAGAAAAATAATGTCGTGATGATCGATAttttcgaat                                                                                     >  1:1232028/1‑145 (MQ=255)
                                                                  ccAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGtgtg                                                                                    <  2:576095/150‑1 (MQ=255)
                                                                  ccAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGtgtg                                                                                    <  2:718386/150‑1 (MQ=255)
                                                                   cAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTAAAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGACAAATTATGTCGTGAGGATCGTTATTTTGGTGTgg                                                                                   >  1:850935/1‑150 (MQ=255)
                                                                     tagTTAACAATGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGAt                                                                                 <  2:455547/149‑1 (MQ=255)
                                                                     gagTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTAAATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGGGATGATCGTTATTTTGGTGTGGAt                                                                                 <  2:742247/150‑1 (MQ=255)
                                                                       attAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTTACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATgg                                                                               >  1:227413/2‑150 (MQ=255)
                                                                         tAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACTATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTa                                                                             >  1:82757/1‑150 (MQ=255)
                                                                          aaCAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATTTCGTGATGATCGTTATTTTGGTGTGGATGGTaa                                                                            >  2:157864/1‑150 (MQ=255)
                                                                            cAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGc                                                                          >  1:574657/1‑150 (MQ=255)
                                                                            cAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGc                                                                          >  2:423988/1‑150 (MQ=255)
                                                                             aaGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCg                                                                         <  2:812327/150‑1 (MQ=255)
                                                                             aaGGAAATATCTCGGAGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCATCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCg                                                                         <  1:619258/150‑1 (MQ=255)
                                                                                    tatCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGa                                                                  >  1:14244/1‑150 (MQ=255)
                                                                                    tatCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATAGTTATTTTGGTGTGGATGGTAAGCGGAGTGGa                                                                  >  1:188460/1‑150 (MQ=255)
                                                                                        tcGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGaaaaa                                                              >  1:1165028/1‑150 (MQ=255)
                                                                                         cGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTTACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAaat                                                             >  2:1181845/1‑150 (MQ=255)
                                                                                         cGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAaat                                                             >  2:990469/1‑150 (MQ=255)
                                                                                          ggCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGCTCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAaatc                                                            >  2:846350/1‑150 (MQ=255)
                                                                                                  aGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAac                                                    <  1:540056/150‑1 (MQ=255)
                                                                                                        tGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACcaccac                                              <  1:1077316/150‑1 (MQ=255)
                                                                                                        tGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATACGCTAACGGTGTATTTTGCGATTTTAAAGAAAAATTATGGCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCTAACcaccac                                              <  2:1313511/150‑1 (MQ=255)
                                                                                                              cTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGAt                                        <  1:939619/150‑1 (MQ=255)
                                                                                                                tACAATCATTACAAGGCTACGATCCAATCGAATTTACATTCGCTAAGGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATTATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGt                                      <  1:1155575/150‑1 (MQ=255)
                                                                                                                tACAATCATTACAAGGCTAAGATCCCAGCGAATTTACATTCGCTAACGGTTTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTTGAAAAATCAATCAATCCACCACAAAGATGt                                      <  1:1170888/150‑1 (MQ=255)
                                                                                                                 aCAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGtt                                     <  2:327440/150‑1 (MQ=255)
                                                                                                                    aTCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGttatt                                  <  1:107511/150‑1 (MQ=255)
                                                                                                                      cATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATCTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGttattta                                <  1:157864/150‑1 (MQ=255)
                                                                                                                        ttACAAGGCTTCGATCCCAGCGAATTTACAATCGCTAACGCTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTCATGATCGTTATTTTTGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTAtg                              <  1:100833/150‑1 (MQ=255)
                                                                                                                        ttACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTAtg                              <  2:519702/150‑1 (MQ=255)
                                                                                                                         tACAAGGCTACGATCCCAGCGAATTTACATTCTCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTAtgt                             <  1:1192410/150‑1 (MQ=255)
                                                                                                                          aCAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTAtgtg                            >  2:684726/1‑150 (MQ=255)
                                                                                                                          aCAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTACGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTAtgtg                            >  1:503705/1‑150 (MQ=255)
                                                                                                                           cAAGGCTACGATCCCAGCGAATTTACATTCGCAAACGGTGTATTTGGGTATGTTAAAGAAAAATTATGAAGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAACACAATCAACACACAACAAAGAACTAATTTATgtgt                           >  2:136549/1‑150 (MQ=255)
                                                                                                                             aGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCg                         <  1:419707/150‑1 (MQ=255)
                                                                                                                             aGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCg                         >  2:76567/1‑150 (MQ=255)
                                                                                                                              ggCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGTGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCgt                        <  1:453027/150‑1 (MQ=255)
                                                                                                                               gCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCgtg                       >  1:1230443/1‑150 (MQ=255)
                                                                                                                               gCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCgtg                       <  2:319731/150‑1 (MQ=255)
                                                                                                                                cTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCgtga                      >  2:434145/1‑150 (MQ=255)
                                                                                                                                   ccaTCCCTGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCgtgagtg                   <  1:581139/148‑1 (MQ=255)
                                                                                                                                   cGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCgtgagtg                   <  2:286048/150‑1 (MQ=255)
                                                                                                                                       gttaGCGAATGTAAATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGt               >  1:1008708/4‑150 (MQ=255)
                                                                                                                                       gttaGCGAATGTAAATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGt               >  1:476415/4‑150 (MQ=255)
                                                                                                                                         cAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTTTTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCt             >  2:780861/1‑150 (MQ=255)
                                                                                                                                         cAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTGAAAGAAAAATTATGTCGTGATGATCGTTAATTTGGTGGGGATGGTAAGCGGAGTGTAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCt             <  2:50362/150‑1 (MQ=255)
                                                                                                                                           gCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTg           <  2:363559/150‑1 (MQ=255)
                                                                                                                                             ctaTTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGtt         <  1:1239136/148‑1 (MQ=255)
                                                                                                                                              aaTTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGttt        <  1:991795/150‑1 (MQ=255)
                                                                                                                                              aaTTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGttt        <  1:581296/150‑1 (MQ=255)
                                                                                                                                                  tACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTCAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACAt    >  1:830598/1‑150 (MQ=255)
                                                                                                                                                    cATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTa  >  1:268510/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTTGGTGTGGATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTA  >  NC_000913/1226687‑1226984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑