Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,217 G→T 15.0% noncoding (20/171 nt) rttR ← rtT sRNA, processed from tyrT transcript

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,2170GT15.0% 99.4 / 4.3 51noncoding (20/171 nt)rttRrtT sRNA, processed from tyrT transcript
Reads supporting (aligned to +/- strand):  ref base G (24/18);  new base T (4/5);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 7.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCT  >  NC_000913/1287068‑1287357
                                                                                                                                                     |                                                                                                                                            
gtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCTGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTg                                                                                                                                              <  2:302712/150‑1 (MQ=25)
     gggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAg                                                                                                                                         <  1:272545/150‑1 (MQ=255)
       gggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCATGGTCGAAGCTTATCAAACATACCCGCAAGGGCAGAATATTTAATTTCGGATTCGTTTGGAAttt                                                                                                                                       >  2:461765/1‑150 (MQ=11)
               ttACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACt                                                                                                                               <  2:926642/150‑1 (MQ=255)
                 aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGGCttt                                                                                                                             >  1:631633/1‑150 (MQ=255)
                 aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGGAGGGGCGGCATATTTAATTGCGGATTCGTGGGGAAGGTCAggggcgtt                                                                                                                             >  1:1278845/1‑145 (MQ=14)
                   ccAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTAGAGTCGAACCTTGTTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTg                                                                                                                           <  1:1027335/149‑1 (MQ=255)
                    cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGACGCATCTCATCCTTCCCCGCATGGGCAGAAAATTTAAGTGGGGAGTCGTTGGGAAGGTCAGGGAGTGTTGa                                                                                                                          >  1:52590/1‑150 (MQ=14)
                    cAGCGCTGAGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGa                                                                                                                          <  2:1087151/150‑1 (MQ=255)
                         cTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGCACCGTGGTCCAAGCATCGAATACGTACCCGCAAGGGGAGAATAATTAATTTCGGATTCGTTTGGAAATTAAGGGACTTTTGAAAGTg                                                                                                                     >  2:1025034/1‑150 (MQ=14)
                                cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCATCTCATACTTCCCCGCATGGGCAGAAAATTTAATTGCGGATTAGTTGGGTAGTTGAGGGACTTTTGAAAGTGAtggtgg                                                                                                              >  2:416821/1‑150 (MQ=14)
                                   cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTATCATCCTTACCCGCATGGGCAGAATATTTAATTTCGGATTAGTTGGGAAGTTAAGGGACTTTTGAAAGTGAttgtggtgg                                                                                                           >  2:466689/1‑150 (MQ=17)
                                     cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTgggg                                                                                                         >  1:761092/1‑150 (MQ=255)
                                       cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATTGTGGTGGGGGa                                                                                                       >  1:113706/1‑150 (MQ=255)
                                                  tgccgcaggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAc                                                                                            >  1:1194833/8‑150 (MQ=255)
                                                   gCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAcc                                                                                           >  1:1103673/1‑150 (MQ=255)
                                                   gCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAcc                                                                                           >  1:794360/1‑150 (MQ=255)
                                                         ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGaa                                                                                     >  1:448865/1‑150 (MQ=255)
                                                         ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCATCACATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATTGTGGTGGGGGAAGGATTCGAACCTTAGaa                                                                                     >  1:1002794/1‑150 (MQ=255)
                                                           cAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGCAGCTTCTCATCCTTCCCCGCCTGGGCCGAATATTTAATTGCGGATTCGTGGGGAAGGTCAGGGACTTTTGAAAGTGAGGGTGGTGGGGGAAGGATTCGAACCTTCGAAGt                                                                                   >  1:822171/1‑150 (MQ=18)
                                                               gCTCTCTCGCTGGCGCTCGATGCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCATAATATTTAATTGCGGATGCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAt                                                                               <  2:1002794/150‑1 (MQ=255)
                                                                    ctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACgg                                                                          >  2:527432/1‑150 (MQ=255)
                                                                      cGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGCCGGCa                                                                        >  1:160747/1‑150 (MQ=255)
                                                                       gCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAg                                                                       >  2:685986/1‑150 (MQ=255)
                                                                           gcgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGTAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAttt                                                                   <  1:112945/150‑1 (MQ=255)
                                                                           gcgcTCGAGGCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGCTGGGCAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAttt                                                                   <  1:1276218/150‑1 (MQ=255)
                                                                                  aGTCGCACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGAGCAGAATATTTAATTGCGGATGCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCt                                                            >  1:205837/1‑150 (MQ=255)
                                                                                    ccGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGGTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGc                                                          <  1:74155/149‑1 (MQ=255)
                                                                                      gAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTg                                                                                                                                              >  2:143140/1‑64 (MQ=255)
                                                                                      gAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTg                                                                                                                                              <  1:143140/64‑1 (MQ=255)
                                                                                              gTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTTGCAAGTTCAGGGACTATTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGc                                                <  1:205203/150‑1 (MQ=255)
                                                                                               tCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGCGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGcc                                               >  1:1142093/1‑150 (MQ=255)
                                                                                                    gCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCg                                          <  1:461765/150‑1 (MQ=255)
                                                                                                     cTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCTAACATTCGAAGTAGATTACGGCAGATTTACAGTCTTCTCCCTATGGACTCTCgg                                         >  1:842919/1‑150 (MQ=21)
                                                                                                              cTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGACCCATCGAAGTCGATCACGGCAGATTTACAGTCAGCTCCCATTGCACGCTCGGGAGcccacc                                >  2:470465/1‑147 (MQ=17)
                                                                                                              cTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCccac                                >  1:442667/1‑150 (MQ=255)
                                                                                                                 ccccGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTATAAGTATCTTAGTGGATGACGGATGATTTAGTGTCTTCTGGCTTAGTACTCTCGGGAAcccacgcat                             >  1:36894/1‑144 (MQ=9)
                                                                                                                   ccGCATGGGCAGAATATTTGATTGCGGATTCGATTGCTAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAggg                           <  2:957357/150‑1 (MQ=21)
                                                                                                                      cATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTa                        >  2:297702/1‑150 (MQ=255)
                                                                                                                        tGGGCAGAATATTTCATTGCGGATTCGTTGGCAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAAt                      <  1:412317/150‑1 (MQ=255)
                                                                                                                        agggCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAGAACATTCTAAGAAGAAGAACGCAGATTTACAGTATTCAACAATTGTCAGATCGGGAACCCCAAAAGCGGTaaa                      >  1:804550/2‑149 (MQ=12)
                                                                                                                            cAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACATTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCACCCAGGGGTAATTaaa                  >  1:278884/1‑150 (MQ=255)
                                                                                                                              gAATATTTCATTGCGGATTCGCTGGCAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAAt                <  1:470465/150‑1 (MQ=255)
                                                                                                                              gAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAAt                <  2:1194833/150‑1 (MQ=255)
                                                                                                                               aaTATATAACTGCGGAGTCGTTGGGCACTGCAGGGACTTTTGAAAGGGATGGTGGTGGGGGAAGGGTTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAAtt               <  1:1067961/150‑1 (MQ=18)
                                                                                                                                atatTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAAttt              <  1:685986/150‑1 (MQ=255)
                                                                                                                                 tatTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAAtttt             <  2:448865/150‑1 (MQ=255)
                                                                                                                                    tttaTTTCGGCTTCGTTTGCTAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAg          <  1:1234914/147‑1 (MQ=18)
                                                                                                                                    ttccttGCGGATTCGGTTGGCACTTCCGGGCCGTTTGAAAGTGATGGGGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAg          <  1:1092606/146‑1 (MQ=18)
                                                                                                                                    tatcttGCGGATTCGTTGGTCATTTCAGGGACTTTTGAAAGTGATGGGGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAg          <  1:1087204/146‑1 (MQ=21)
                                                                                                                                       aTTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGa                                                                                >  1:72439/1‑77 (MQ=11)
                                                                                                                                       aTTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGa                                                                                <  2:72439/77‑1 (MQ=11)
                                                                                                                                            ggATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCt  <  1:297702/150‑1 (MQ=255)
                                                                                                                                            ggATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCt  <  2:794360/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                            
GTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCT  >  NC_000913/1287068‑1287357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑