Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 126,384 C→T 9.1% D230D (GAC→GAT aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913126,3840CT 9.1% 189.2 / 6.0 69D230D (GAC→GATaceFpyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
Reads supporting (aligned to +/- strand):  ref base C (40/20);  new base T (4/2);  total (44/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GTGAAGCAGGCGCGGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGATCTTCGAAGT  >  NC_000913/126236‑126533
                                                                                                                                                    |                                                                                                                                                     
gTGAAGCAGGCGCGGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTGCGGATATAGGCTGTGAAGTAGTTGAAGTGACTGAAGTGATTGTGAAAGTGGGCGACa                                                                                                                                                      >  2:528107/1‑150 (MQ=255)
 tGAAGCAGGCGCGGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACaa                                                                                                                                                     >  2:448985/1‑150 (MQ=255)
 ggacgCAGGCGCGGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACaa                                                                                                                                                     <  2:796141/146‑1 (MQ=255)
     gCAGGCGCGGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGCCGGGAAAGAAGATAAAGTTCCGGAAATCGGAGGTGACGAAGTTGAAGTGAATGAAGTGATGGTGAAAGTGGGAGACAAAGtt                                                                                                                                                 >  2:1240814/1‑150 (MQ=255)
       aGGCGCGGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCGCTGCACCAGCGGCAGCGGCGCAAGAAGCAAAAGATCCAGATAACGGCGGTGAGGAAGTTGATGGGACTGAAGAGATGGTGAAAGGGGGACACaaaagtgc                                                                                                                                               >  2:112743/1‑145 (MQ=255)
         gcgcGGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCCGCTGGCGGGAAAGAAGAAACCGATCACGATATAGGCATAGACGAAGTTGAAGTTACAGAAGGGAAGGAGAAAGTGGGCGTCAAAGTTTCCg                                                                                                                                             >  2:1258554/1‑150 (MQ=255)
              gCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGaa                                                                                                                                        >  2:950995/1‑150 (MQ=255)
                 gCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGATGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAg                                                                                                                                     >  1:540743/1‑150 (MQ=255)
                 gCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACAGAAGAGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAg                                                                                                                                     >  2:636601/1‑150 (MQ=255)
                  cTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGGTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGt                                                                                                                                    >  2:860899/1‑150 (MQ=255)
                   tCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTc                                                                                                                                   <  2:1283535/150‑1 (MQ=255)
                       gCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGGAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTg                                                                                                                               >  1:43634/1‑150 (MQ=255)
                          gCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATc                                                                                                                            >  2:396619/1‑150 (MQ=255)
                             aaaCAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCAcc                                                                                                                         <  2:775166/150‑1 (MQ=255)
                                cAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTa                                                                                                                      >  1:1245809/1‑150 (MQ=255)
                                 aGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAg                                                                                                                     <  2:1104023/150‑1 (MQ=255)
                                  ggAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGa                                                                                                                    >  1:1042608/1‑150 (MQ=255)
                                       cagcTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCg                                                                                                               >  1:1173800/1‑150 (MQ=255)
                                            ccGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACaaa                                                                                                          >  2:262878/1‑150 (MQ=255)
                                             cGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAg                                                                                                         <  1:406923/150‑1 (MQ=255)
                                               gCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGATGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCt                                                                                                       >  2:640604/1‑150 (MQ=255)
                                                  gCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGAAAAAGCTTCt                                                                                                    >  2:818384/1‑150 (MQ=255)
                                                     gCCCCTGCACCAGAGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATg                                                                                                 >  1:873101/1‑150 (MQ=255)
                                                              ccAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCCCCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCg                                                                                        >  2:888982/1‑150 (MQ=255)
                                                              ccAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCg                                                                                        >  1:929007/1‑150 (MQ=255)
                                                                  cggcggGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACACAGCTTCTATGGAAGTTCCGgcgc                                                                                    >  1:279117/1‑150 (MQ=255)
                                                                      tGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCggt                                                                                >  2:202745/1‑148 (MQ=255)
                                                                       ggCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGttt                                                                               >  1:861220/1‑150 (MQ=255)
                                                                         cGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGc                                                                             >  2:1022325/1‑150 (MQ=255)
                                                                          gTGAAAGAAGTTAAAGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGTCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCa                                                                            >  2:255248/1‑150 (MQ=255)
                                                                              aagacgTTAACGTTCCGGATATCGGCGGCGACGACGTTGAAGTGACTGAAGTCAGGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCCCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGcg                                                                        <  1:1286930/150‑1 (MQ=17)
                                                                                 aagTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGATGCCGCTGAACAGTCACTGATCCCCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGATTGCAGGcgtcc                                                                     >  2:571892/1‑149 (MQ=255)
                                                                                   gTTAACGTTCCGGATATCGGCTGCGACGAAGTTGAAGTGACCGAAGTGCTGGTGAAAGTTGGCGAAAAAGTTGACGCTGAACAGTCTCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTg                                                                   <  2:523074/150‑1 (MQ=11)
                                                                                   gTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTg                                                                   >  2:1085856/1‑150 (MQ=255)
                                                                                       aCGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAgg                                                               <  1:756957/150‑1 (MQ=255)
                                                                                        cGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGATGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGa                                                              >  1:172137/1‑150 (MQ=255)
                                                                                           tCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACt                                                           <  1:1062352/150‑1 (MQ=255)
                                                                                           tCCGGATATCGGCCGAGACGAAGTTGAAGTGACCTAACTGATGGTGAAAGTGGGCGACAAAGTTGAAGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACt                                                           <  1:592361/150‑1 (MQ=17)
                                                                                            ccGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTg                                                          <  1:743189/150‑1 (MQ=255)
                                                                                             cGGATATCGGCGGTGACGAAGTAGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCAGTTTGCAGGCGTCGTGAAGGAACTGa                                                         >  1:854564/1‑150 (MQ=255)
                                                                                              ggATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGACGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGATGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTgta                                                        >  2:216093/1‑148 (MQ=255)
                                                                                              ggATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGGGAAAGGGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGaa                                                        <  2:861468/150‑1 (MQ=255)
                                                                                                atatCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAg                                                      <  1:881100/150‑1 (MQ=255)
                                                                                                atatCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAg                                                      <  2:481787/150‑1 (MQ=255)
                                                                                                     ggcggTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAAc                                                 >  1:1121785/1‑150 (MQ=255)
                                                                                                       cggTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGt                                               >  1:1050607/1‑150 (MQ=255)
                                                                                                                 gTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGATGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATaaa                                     >  1:545409/1‑150 (MQ=14)
                                                                                                                   tGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGt                                   >  1:267236/1‑150 (MQ=255)
                                                                                                                     aaGTGACTGAATTGATGGTGAAAGTGGGCGAAAAAGTTGACGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGa                                 <  1:633056/150‑1 (MQ=255)
                                                                                                                     aaGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGa                                 <  2:545409/150‑1 (MQ=14)
                                                                                                                         gACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAAc                             >  2:270254/1‑150 (MQ=255)
                                                                                                                             gAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGc                         >  2:156743/1‑150 (MQ=255)
                                                                                                                             gAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGc                         >  1:1100873/1‑150 (MQ=255)
                                                                                                                             gAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGc                         <  2:685101/150‑1 (MQ=255)
                                                                                                                                  gATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCt                    >  2:1094404/1‑150 (MQ=255)
                                                                                                                                     ggTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCTCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCg                                          <  1:899928/125‑1 (MQ=17)
                                                                                                                                     ggTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCg                                          >  2:899928/1‑125 (MQ=17)
                                                                                                                                      gTGAAAGTGGGCGAGAAAGTTGCCGCTGAACAGTCACTGATCACCGTATAAGGCGACAACTCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATATAGTGAAAACTGGCTCGCTGAtt                <  1:992254/150‑1 (MQ=255)
                                                                                                                                      gTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGCTCACCGTAGCAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGATGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGAtt                >  1:661098/1‑150 (MQ=255)
                                                                                                                                      gTGAAAGTGGGCGACAAAGTTGACGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGAtt                <  1:423705/150‑1 (MQ=255)
                                                                                                                                       tGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTa               <  1:262878/150‑1 (MQ=255)
                                                                                                                                        gAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTAt              <  1:154810/150‑1 (MQ=255)
                                                                                                                                          aagttcgCGACATAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGa            <  1:1023264/144‑1 (MQ=255)
                                                                                                                                          aaGTGGGCGACAAAGTTGCCGCTGAACAGTCACTTATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGa            <  1:636601/150‑1 (MQ=255)
                                                                                                                                          aaGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGa            >  2:815503/1‑150 (MQ=255)
                                                                                                                                            gttggCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCAGGCGTCGTGAAGGAACTGGAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGATc          >  2:430247/4‑150 (MQ=17)
                                                                                                                                            gttggCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGCAAGTTCCGGCTCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGAGATAAAGTGAAAACTGGCTCGCTGATTATGATc          >  2:904869/4‑150 (MQ=17)
                                                                                                                                                gCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCCACGTTGGCGATAAAGTGAAAACTGGCGAGCTGATTATGATCTTCg      >  1:186655/1‑150 (MQ=255)
                                                                                                                                                 cGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGATCTTCGa     <  1:950995/150‑1 (MQ=255)
                                                                                                                                                    cAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGATCTTCGAAGt  <  2:475254/150‑1 (MQ=255)
                                                                                                                                                    cAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGATCTTCGAAGt  <  2:929007/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTGAAGCAGGCGCGGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGATCTTCGAAGT  >  NC_000913/126236‑126533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: