Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,466,144 G→T 15.7% pseudogene (753/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,466,1440GT15.7% 172.7 / 14.8 79pseudogene (753/2513 nt)ydbApseudogene, autotransporter homolog; interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base G (22/44);  new base T (6/7);  total (28/51)
Fisher's exact test for biased strand distribution p-value = 5.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATC  >  NC_000913/1466000‑1466288
                                                                                                                                                |                                                                                                                                                
aGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCa                                                                                                                                             <  2:964852/150‑1 (MQ=255)
aGATGGCGCAACGAAGTGGAAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCa                                                                                                                                             <  2:410161/150‑1 (MQ=255)
 gATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAg                                                                                                                                            >  2:497485/1‑150 (MQ=255)
    ggCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGAc                                                                                                                                         <  1:333393/150‑1 (MQ=255)
    ggCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGGGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGAc                                                                                                                                         <  2:1239270/150‑1 (MQ=255)
     gcgcAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACg                                                                                                                                        <  2:655774/150‑1 (MQ=255)
       gcAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGc                                                                                                                                      <  1:602633/150‑1 (MQ=255)
             aattGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTg                                                                                                                                <  2:537465/147‑1 (MQ=255)
                 ggCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATg                                                                                                                            <  1:878522/150‑1 (MQ=255)
                 ggCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGCAATGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATg                                                                                                                            <  2:93767/150‑1 (MQ=255)
                       ataATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGATGGCGATCTGGATGTCAgcg                                                                                                                      <  1:366461/150‑1 (MQ=255)
                            cacaACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGCTTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAgcggcggc                                                                                                                 <  2:807476/150‑1 (MQ=255)
                            cacaACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATATGGATGTCAgcggcggc                                                                                                                 >  1:361158/1‑150 (MQ=255)
                            cacaACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGATATTCAGGACGGCGATCTGGATGTCAgcggcggc                                                                                                                 >  2:237263/1‑150 (MQ=255)
                               aaCGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGt                                                                                                              <  1:134619/150‑1 (MQ=255)
                               aaCGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGt                                                                                                              <  1:1198527/150‑1 (MQ=255)
                               aaCGGTGAGCTTGTTATTACGGGGGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGt                                                                                                              <  2:646299/150‑1 (MQ=255)
                                         ttGTTATTAAGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGCCTGTAAAGATTCCACCGGTACGGAAATCAACGGTAATACCGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTg                                                                                                    <  2:941687/150‑1 (MQ=255)
                                             tattaCGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGatat                                                                                                >  2:917372/1‑150 (MQ=255)
                                             tattaCGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGatat                                                                                                <  2:761933/150‑1 (MQ=255)
                                             tattaCGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGt                                                                                                              <  1:245220/136‑1 (MQ=255)
                                             tattaCGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGt                                                                                                              >  2:245220/1‑136 (MQ=255)
                                                taCGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCAc                                                                                             <  1:1077768/150‑1 (MQ=255)
                                                 aCGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACgg                                                                                                         <  1:939107/137‑1 (MQ=255)
                                                 aCGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACgg                                                                                                         >  2:939107/1‑137 (MQ=255)
                                                  cGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCg                                                                                           <  2:444414/150‑1 (MQ=255)
                                                    ggTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGt                                                                                         >  2:982217/1‑150 (MQ=255)
                                                    ggTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGt                                                                                         >  2:942788/1‑150 (MQ=255)
                                                    ggTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGt                                                                                         >  2:823963/1‑150 (MQ=255)
                                                         tAATGCCACAGCAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAg                                                                                    >  1:903653/1‑150 (MQ=255)
                                                              ccACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGTAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTg                                                                                                    <  2:191798/129‑1 (MQ=255)
                                                              ccACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTg                                                                                                    >  1:191798/1‑129 (MQ=255)
                                                               cacaGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGAc                                                                              <  1:242815/150‑1 (MQ=255)
                                                                 caGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGAGCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACgg                                                                            <  1:983574/150‑1 (MQ=255)
                                                                  aGTACACAACACTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCACCGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGt                                                                           <  1:238654/150‑1 (MQ=255)
                                                                  aGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATc                                                                                                                                  <  1:1134758/95‑1 (MQ=255)
                                                                  aGTAAACAACAATGGCAAAACCACCGTTGACGGAAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATc                                                                                                                                  >  2:1134758/1‑95 (MQ=255)
                                                                  aGTAAACAACAATGGCAAAACCACCGTGGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTTTTGATATCACCGGTGACAGCGCGACGGt                                                                           <  1:292167/150‑1 (MQ=255)
                                                                   gTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGa                                                                                                                              >  2:51556/1‑98 (MQ=255)
                                                                   gTAAACAACAATGGCAAAACCACCCTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGACCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTg                                                                          <  1:44306/150‑1 (MQ=255)
                                                                    tAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTgg                                                                         <  2:879853/150‑1 (MQ=255)
                                                                      aacaacAATGGCAAAACCACCGTTGCCGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGCCGGTGGAt                                                                       >  1:575668/1‑150 (MQ=255)
                                                                      aacaacAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGAATTGATATCACCGGTGACAGCGCGACGGTGGAt                                                                       >  1:536789/1‑150 (MQ=255)
                                                                          acaaTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATACCa                                                                   >  1:1246942/1‑150 (MQ=255)
                                                                          acaaTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACa                                                                   <  1:1045772/150‑1 (MQ=255)
                                                                           caaTGGCAAAACCACCGATGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACaa                                                                  <  1:957/150‑1 (MQ=255)
                                                                            aaTGGCAAAACCACCGTTGACGGTAAAGACTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAg                                                                 <  1:147803/150‑1 (MQ=255)
                                                                             aTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGTGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAgg                                                                <  2:1182093/150‑1 (MQ=255)
                                                                              tGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGt                                                                           <  2:44249/138‑1 (MQ=2)
                                                                              tGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGt                                                                           >  1:44249/1‑138 (MQ=25)
                                                                               ggCAAAACCACCGTTGACGGTAAAGATGCCACCGTTACGGAAATCAACGGTAATAACGGGACAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGc                                                              <  1:996070/150‑1 (MQ=255)
                                                                                   aaaCCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGTAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACg                                                                                                          <  2:132780/102‑1 (MQ=12)
                                                                                   aaaCCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACg                                                                                                          >  1:132780/1‑102 (MQ=255)
                                                                                    aaCCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGAGACAGCGCGACGGTGGATAACAAGGGCACCAt                                                         >  1:793568/1‑150 (MQ=255)
                                                                                       caccGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGAc                                                      <  1:1267315/150‑1 (MQ=255)
                                                                                         ccGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCATCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCg                                                    <  2:57418/150‑1 (MQ=255)
                                                                                         ccGGTGACGGAAACGACTCCACCGGTACCGACATCATCGGTAATAACGGTAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCg                                                    <  2:1273974/150‑1 (MQ=11)
                                                                                             tGACGGTAAAGATTCCACCGGTACGGAAATCAACTGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCAc                                                <  2:659819/150‑1 (MQ=255)
                                                                                              gACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCAcc                                               >  2:1295574/1‑150 (MQ=255)
                                                                                              gACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCAcc                                               <  2:176709/150‑1 (MQ=255)
                                                                                                    aaaGATTCCACCGGTACGGAAATCAACGGAAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACATCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCg                                         <  1:1031429/150‑1 (MQ=255)
                                                                                                     aaGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCgg                                        >  1:974144/1‑150 (MQ=40)
                                                                                                             aCCGGTACGGAAATCACCGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATg                                <  1:192090/150‑1 (MQ=255)
                                                                                                             aCCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATg                                <  1:404901/150‑1 (MQ=40)
                                                                                                               aggTACGGAAATCAACTGTAATATCGGGAAAGGGATTCAGGTCGGCGAGCTGGATGTCAGCGTCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATggg                              <  2:536789/149‑1 (MQ=14)
                                                                                                                ggTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGt                             <  1:1150316/150‑1 (MQ=255)
                                                                                                                ggTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGt                             >  2:756216/1‑150 (MQ=255)
                                                                                                                     ggAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGAGCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCa                        <  2:805324/150‑1 (MQ=255)
                                                                                                                      gAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGCTCACCATGACCGTCACCGATCCGGAGTCCATAGGTATCCAg                       >  2:1253372/1‑150 (MQ=255)
                                                                                                                           cAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAggtaga                  >  1:1304528/1‑145 (MQ=255)
                                                                                                                           cAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGa                  >  2:609553/1‑150 (MQ=255)
                                                                                                                           cAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGa                  >  2:167890/1‑150 (MQ=255)
                                                                                                                             aCGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGCCg                >  1:1087659/1‑150 (MQ=255)
                                                                                                                               gggaataaCGCGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACGATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGt              <  2:867519/147‑1 (MQ=255)
                                                                                                                                gTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTg             <  1:917372/150‑1 (MQ=255)
                                                                                                                                     aaCGGGAAAGTGATGCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAg        <  2:1311061/150‑1 (MQ=255)
                                                                                                                                     aaCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAg        >  2:146457/1‑150 (MQ=255)
                                                                                                                                       cGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGGATTGATCTCCCCGGTGACAGCGCGACGGCGGATAACAAGGGCACCATGACCGGCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGGGACAAGGc      <  1:65791/150‑1 (MQ=14)
                                                                                                                                           aaaGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATc  <  2:575668/150‑1 (MQ=255)
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AGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATC  >  NC_000913/1466000‑1466288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: