Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,466,336 | C→G | 13.5% | pseudogene (945/2513 nt) | ydbA → | pseudogene, autotransporter homolog; interrupted by IS2 and IS30 |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,466,336 | 0 | C | G | 13.5% | 136.7 / 6.9 | 56 | pseudogene (945/2513 nt) | ydbA | pseudogene, autotransporter homolog; interrupted by IS2 and IS30 |
Reads supporting (aligned to +/- strand): ref base C (25/23); new base G (5/3); total (30/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.12e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTAT > NC_000913/1466189‑1466482 | tGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCa < 2:903653/150‑1 (MQ=255) gATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCGGGAGTCCATGGGTAACCAGAACGACGGTGACAAGGCCATCGTCAATTACGAAGGCGAGCGCCCCAACACCAACGGTGGCTCCGGCACCCAg > 2:522561/1‑150 (MQ=17) gATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAg < 2:299320/150‑1 (MQ=255) gATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATAGACGGAGACAAGGCCATAGTAAAAAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAg > 2:1126650/1‑150 (MQ=14) atCACCGGTGCCAGCGCGACGGTGGATAACAATGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAATGCCATCGTCAATAACGAAGGCGATAGCACCATCTCGAACGGTGGCACCGGCACCCAGAtt < 2:408122/150‑1 (MQ=17) atCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGCTCGACGGTGACAAGGCCATCGTCAAAAACGAAGGCGAGAGCACCATCACCACCGGTGGCACCGGCACCCAGCtt > 1:1125551/1‑150 (MQ=255) tCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTa < 1:418304/150‑1 (MQ=255) tCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTa > 1:410280/1‑150 (MQ=255) cACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATAGACGGGGACAAGGCCATCGTCAAAAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTaa > 1:1321711/1‑150 (MQ=255) ccGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATg < 2:974144/150‑1 (MQ=255) cGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATgg < 1:823963/150‑1 (MQ=255) cGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATgg < 1:942788/150‑1 (MQ=255) cGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATAGACGGTGACAAGGCCATAGTCAAAAACGAAGGCGAGAGCCCCATCACCAACGGTGGCACCGGCACGCAGATTAAAGGTGATGACGCCACgg > 1:909056/1‑150 (MQ=14) gATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATAGAAGGTCACAAGACCATCGTCAATTACTAAGGAGAAATCACCATAACAAAAGGTGGCACCGGCAACCATATTAAAAGTGATGACGCCACgggagac > 2:1000745/1‑145 (MQ=17) aCAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTGAAGAACGAAGGCGAGAGCACCATCCCCAACGGTGGCACCGGCACCCAGAGTAAGGGGGCTGCCGCCACGGCAaacaaca > 1:1341749/1‑150 (MQ=17) gggCACCATGACCGTCACCGATCCTGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACgg < 1:333076/150‑1 (MQ=255) ggCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGc > 2:222168/1‑150 (MQ=255) gCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCa < 1:146457/150‑1 (MQ=255) ccATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGGGATGACGCCACGGGAAACAACAACGGCaaaa > 2:984459/1‑150 (MQ=255) cATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGCGAGCACCATCACCAACGCTGGCACCGGCCCCCAGATTACTGGTGATGACGCCACGGCAAACAACAACGGCAAAac > 1:450023/1‑150 (MQ=255) cATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAac < 2:435652/150‑1 (MQ=255) cATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAac > 1:609570/1‑150 (MQ=255) acagTCACCGATCAGGATTCCATGGGTATCAAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGAGAGAGCACCATCACCAACGTTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAaccacc < 2:418745/147‑1 (MQ=18) ccGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCg < 1:1295574/150‑1 (MQ=255) ccGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCg < 2:1246942/150‑1 (MQ=255) gTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCCCCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGtt > 2:393323/1‑150 (MQ=255) tCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGCGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTg > 2:451011/1‑150 (MQ=255) aCCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGAc > 2:577051/1‑150 (MQ=255) gATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGc < 1:756216/150‑1 (MQ=255) aTCCGGTGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCa > 2:516715/1‑150 (MQ=255) cGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAgg < 2:1087659/150‑1 (MQ=255) atcggcaTCCAGCGCGACGGAGACAAGGCCATCGTCAACAACGAAGGCGAGAGCCCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAcc < 2:1341749/144‑1 (MQ=11) aTCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACg > 1:1032380/1‑150 (MQ=255) gATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGACCCGGCAACCAACAACGGCAAAACCAACGAAGACGGCAAGGATTCCACCGGTACGGAAAt > 1:5977/1‑150 (MQ=17) gACGGTGACAAGGCCATCGTCAATAACGAATGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTATTTGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCATGGATTCCACCGGTACGGAAATCAAc < 1:1253372/150‑1 (MQ=255) gACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGtaat < 2:222515/150‑1 (MQ=255) cAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAAAGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTAc > 1:110447/1‑132 (MQ=255) cAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAAAGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTAc < 2:110447/132‑1 (MQ=255) aGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGAGGCACCGGCACCCAGATTAAAGGTGATGACGCCACCGCAAACAACAACGGCAACCCCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATACACGTCAATAACg > 1:365007/1‑150 (MQ=14) cGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGAAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATACCAGGAAATAACGGGAAGGt > 2:727477/1‑150 (MQ=11) cAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaacaac < 2:1269216/79‑1 (MQ=9) cAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaacaac > 1:1269216/1‑79 (MQ=9) cAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaaca > 2:99401/1‑77 (MQ=9) cAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaaca < 1:99401/77‑1 (MQ=9) aaCGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTTATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAg < 1:222168/150‑1 (MQ=255) aCGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGTCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAgg > 2:1294439/1‑150 (MQ=255) aCGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAgg > 1:649487/1‑150 (MQ=255) aCGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTAGACGGCAAGGATTCCACCGGTACCGAAATCAACGGTAATAACGGAAAAGTTAGTCAgg > 1:231809/1‑150 (MQ=255) cGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGa < 2:410280/150‑1 (MQ=255) cccATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGAt < 1:522561/149‑1 (MQ=255) ccATCACCAACTGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACTGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATg < 1:577051/150‑1 (MQ=255) cACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACCACAACGGCAAAACCACCGTTGCCGGCAAGGATTCCACCGGTACGGCAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGCTCGGCATGTCac > 1:243357/1‑149 (MQ=17) cACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAg > 1:704268/1‑150 (MQ=255) ccAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCgg > 1:714651/1‑91 (MQ=37) ccAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCgg < 2:714651/91‑1 (MQ=37) ccAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATGAAGGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcg > 2:432818/1‑150 (MQ=255) accggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAc < 2:1352979/150‑1 (MQ=255) ggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGt > 1:529275/1‑150 (MQ=255) caccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTa > 1:1113161/1‑77 (MQ=39) caccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTa < 2:1113161/77‑1 (MQ=39) caccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTAt < 1:451011/150‑1 (MQ=255) cccAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAg > 2:497798/1‑62 (MQ=39) cccAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAg < 1:497798/62‑1 (MQ=39) | TGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTAT > NC_000913/1466189‑1466482 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |