Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,552,191 C→A 23.4% intergenic (+200/+207) fdnI → / ← yddM formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,552,1910CA23.4% 70.4 / 7.2 50intergenic (+200/+207)fdnI/yddMformate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (14/23);  new base A (10/3);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 2.36e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCAT  >  NC_000913/1552042‑1552340
                                                                                                                                                     |                                                                                                                                                     
atatTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGAAATGTTGCGTTTGTCATCAGTTTc                                                                                                                                                       >  1:1108715/1‑150 (MQ=14)
 tatTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCa                                                                                                                                                      <  2:1262121/150‑1 (MQ=32)
    tGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAAt                                                                                                                                                   <  2:432106/150‑1 (MQ=34)
    tGTAGACAACGTAGGCTTTGATCATGCCGGATGCGGGGTGAACGCCTTATCCGGCATGAAAACCCATCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAAt                                                                                                                                                   <  1:878675/150‑1 (MQ=255)
           aaCGTAGGCTTTGTTCATGCCGGAGGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTg                                                                                                                                            <  2:864190/150‑1 (MQ=25)
             cGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTa                                                                                                                                          <  1:1270810/150‑1 (MQ=37)
                   cTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGc                                                                                                                                    <  2:422407/150‑1 (MQ=255)
                    tttGTTCAGGCCGGATGCGGCGGGAACGCCTTAACCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGCACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCg                                                                                                                                   <  2:205515/150‑1 (MQ=255)
                       gTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTTATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCa                                                                                                                                <  2:61220/150‑1 (MQ=255)
                              ccGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTTAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCat                                                                                                                         <  1:1157427/150‑1 (MQ=255)
                                    gcggcgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTa                                                                                                                   <  2:304946/150‑1 (MQ=255)
                                       gcgTGAACGCCTTATCCGGCATTAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGAAATGTTGCGTTTGGGATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGAc                                                                                                                <  2:727325/150‑1 (MQ=255)
                                        cgTGAACGCCTTATCCGGAATGAAAACCCTTCAAATCCTATAGATTGCAGTGAACGTGTAGGCCTGATATGCGTATCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACa                                                                                                               <  2:26374/150‑1 (MQ=255)
                                           gAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTAAAAGGAGTCATTTTCATATTGAATACAACg                                                                                                            >  2:866982/1‑150 (MQ=255)
                                            aaCGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGAAGACAACGt                                                                                                           >  1:1329527/1‑150 (MQ=255)
                                              cGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTAAAAATGTCTCTGTAAAAAGAGGCATTTTCATATTGAATACACAGTAg                                                                                                         >  2:272062/1‑150 (MQ=11)
                                                ccTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGACTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTAAAAATGGCGCTGTAAAATGAGACATTTTAATATTGTATAAAAAGAAGGc                                                                                                       >  2:1350404/1‑150 (MQ=11)
                                                 cTTAACCTGCATGAAAACCCTTCAAATCCAATAGATTGCAGTTAACGTGTAGGCCTGATAATCTTAGCGAATCATGAAATGTTGCGTTTGTAATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCt                                                                                                      <  2:541949/150‑1 (MQ=18)
                                                  ttATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTTAACGTGTAGGCCTGATAAGAGTAGCGCATAAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCtt                                                                                                     <  2:610070/150‑1 (MQ=255)
                                                    aTCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTg                                                                                                   <  1:1106735/150‑1 (MQ=255)
                                                                 aCCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGCCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTATTAAGCGTCATTTTCATATTGTTTAAAAAGTAGGCTTTGTTCATCCCgattc                                                                                      >  1:310527/1‑146 (MQ=17)
                                                                            ccAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGAGTCATTTTCATATTGTATACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAAcc                                                                           >  2:994439/1‑149 (MQ=255)
                                                                                 aGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTAAAAATGGCGCTGTAAAAGGAGTCATTTTAATATTGAAGACACCGAAGAAATTGATCAAGACGGATTCGGAATGaaagaatta                                                                      >  2:22489/1‑143 (MQ=14)
                                                                                   aTTGCATTGAACGTGTAGTCCTGATAAGCGTATCGCTTGAGGAAATGTTGAGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATc                                                                    <  2:1302564/150‑1 (MQ=18)
                                                                                    ttGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATcc                                                                   <  2:1108715/150‑1 (MQ=255)
                                                                                        aGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGGGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATACCGGATGCGGCGTGAAAGCCTTATACGGCa                                                               >  1:1340710/1‑150 (MQ=25)
                                                                                         gTGAACGTGTAGGCCTGATAACCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGGTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCAt                                                              <  1:325988/150‑1 (MQ=255)
                                                                                          tGAACGTGTCGTCCTGATAAGCGTAGCGAATCAGGAAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATg                                                             <  2:467453/150‑1 (MQ=255)
                                                                                               gtgtAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAAc                                                        >  2:1337027/1‑150 (MQ=255)
                                                                                               gtgtAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAAAGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAAc                                                        <  2:289453/150‑1 (MQ=255)
                                                                                                  tAGGCCTGATAAGCTAAGCGAATGAGGCAATGTTGCGTTTGGCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATTCCGGATGCGTCGTGAACGCCTTATCCGGCATGAAAACCCt                                                     <  2:1217753/150‑1 (MQ=18)
                                                                                                   aGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTAATATTGTAGACAACGTAGGCTTTGTTCATGCAGGATGCGGCGTGATACACATATCCGGAAAGAAAACCCtt                                                    >  1:134558/1‑150 (MQ=21)
                                                                                                    gtcttGATAATCGTATCGCATGAGGCAATGTTGTTTTTTTAATTAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTc                                                   <  2:205128/146‑1 (MQ=14)
                                                                                                    ggCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTc                                                   >  2:1279800/1‑150 (MQ=255)
                                                                                                    ggCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTc                                                   >  2:389805/1‑150 (MQ=255)
                                                                                                     gCCTGCTACGCGGAGCGCATGAGGCATTGTTGCTTTTGTGATTAGATGCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCTGCATGAAAACCCTTCa                                                  <  2:1036079/150‑1 (MQ=14)
                                                                                                     cccTTATAATCGTACCGCATCAGGCAATGTCGCGTTTTCCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCa                                                  <  1:16882/149‑1 (MQ=17)
                                                                                                      ccTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTATACAAAGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACTCCTTATACCGCATGAAAACCCTTCaa                                                 >  2:334613/1‑150 (MQ=21)
                                                                                                         gATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGCGACATTTTAATATTGAAGACAAAGTAGGCTTATTTCATGCCCGAAGAGTAGTGAAAGACTTATAAGGCATGAAAAACAATCAAATc                                              >  2:536911/1‑150 (MQ=18)
                                                                                                              tcGTAGCGCATCTGTTAATGTTTTGTTTGTTATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCTTATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATa                                         <  2:1074572/149‑1 (MQ=17)
                                                                                                                  aGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGGCATTTTCATATTGTAGACAACGTAGGCATTGTACATGCCGGATGCGGCGTGAACGACTTATCCGGCATGAAAACCCTTGAAATACAATAGAtt                                     >  2:1012930/1‑150 (MQ=18)
                                                                                                                     gcATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACCTAGGCTTTCTACATGCAGTAAGCCTCGTGAACGCCATATCCGGAAAGAAAACCCTTCAAAACCAATAGATTGCa                                  >  2:1189617/1‑150 (MQ=17)
                                                                                                                      cATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATACGGCATGAAAACCCTTCAAATCCAATAGATTGCAg                                 >  1:401976/1‑150 (MQ=255)
                                                                                                                          aGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGaa                             >  1:1001592/1‑150 (MQ=255)
                                                                                                                             cAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACgt                          >  2:1340696/1‑150 (MQ=255)
                                                                                                                                 gTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAg                      >  1:1283499/1‑150 (MQ=255)
                                                                                                                                 gTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTAATATTGTAGACAACGTAGGCTTTGTTCATTCCCGATGCGGCGTGAACGCCTTAACCGGCATGAAAACCCTTCAAATCCAATAGAATGCAGTGAACGTGTAg                      >  2:1066116/1‑150 (MQ=17)
                                                                                                                                      gTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTg                 >  2:1047385/1‑150 (MQ=255)
                                                                                                                                            catcaGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAAAACTTCAGAACCAATAGAATGCAGTTAACGTGTAGTCCTGATAAGc           >  1:920881/1‑150 (MQ=14)
                                                                                                                                                 gTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGc                                   <  1:1049107/121‑1 (MQ=18)
                                                                                                                                                  tttCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAgcg     >  2:123405/1‑150 (MQ=35)
                                                                                                                                                     cAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTCGGCCTGATAAGCGTAGCGCAt  >  2:352356/1‑150 (MQ=17)
                                                                                                                                                     cAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCAt  >  2:639495/1‑150 (MQ=32)
                                                                                                                                                     |                                                                                                                                                     
ATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCAT  >  NC_000913/1552042‑1552340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑