Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,700,361 G→A 7.2% G336D (GGC→GAC)  malX → maltose and glucose‑specific PTS enzyme IIB component and IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,700,3610GA 7.2% 174.3 / 2.2 56G336D (GGC→GAC) malXmaltose and glucose‑specific PTS enzyme IIB component and IIC component
Reads supporting (aligned to +/- strand):  ref base G (31/20);  new base A (2/2);  total (34/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

ACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGG  >  NC_000913/1700213‑1700510
                                                                                                                                                    |                                                                                                                                                     
aCGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCCGCGGGCTGCCCGGTGCAGCGTTAGCTATCTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTggcggc                                                                                                                                                      <  2:357360/150‑1 (MQ=255)
 cgcgTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAGATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCa                                                                                                                                                     >  1:409954/1‑150 (MQ=255)
   cgTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACt                                                                                                                                                   >  1:387206/1‑150 (MQ=255)
    gTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAAATAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTa                                                                                                                                                  >  1:1158579/1‑150 (MQ=255)
     tttCCTTTCGCAAGGTAAAATGCCTGCGTTTGTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCGATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTAc                                                                                                                                                 <  2:112930/150‑1 (MQ=255)
      ttCCTTTCGCAAGGTAAAATGCCTGCGGTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTAcc                                                                                                                                                <  1:1089157/150‑1 (MQ=255)
       tCCTTTCGCAAGGTAAAATGCCTGCGGTTGTGGGCTGTCTGCCAGGTGCAGCGTTAGCTATGTATAACTGCGCGGGCCAGGAAAATCGCCATAAATTTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTAGTTTGCGGCACTACCg                                                                                                                                               <  2:573421/150‑1 (MQ=255)
        ccTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTTTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGa                                                                                                                                              >  1:1307350/1‑150 (MQ=255)
           ttCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAAcc                                                                                                                                           <  1:1054120/150‑1 (MQ=255)
             cGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGc                                                                                                                                         >  1:1041526/1‑150 (MQ=255)
              gCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGcg                                                                                                                                        >  2:1156889/1‑149 (MQ=255)
                   gTAAAATTCCGGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAAt                                                                                                                                   <  1:1002383/150‑1 (MQ=255)
                      aaaTGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGTCCCTACAGAACCGCTGGAAttcc                                                                                                                                >  2:1184431/1‑150 (MQ=255)
                      aaaTGCCTGCGGTGCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAAttcc                                                                                                                                <  1:661205/150‑1 (MQ=255)
                            cTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAAttcctgttcc                                                                                                                          >  1:942309/1‑150 (MQ=255)
                                    ctcGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTa                                                                                                                  >  2:795062/1‑150 (MQ=255)
                                      cggcggTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCTCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAgc                                                                                                                <  2:179336/150‑1 (MQ=255)
                                       ggcggTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAgcg                                                                                                               >  2:1070240/1‑150 (MQ=255)
                                         cggTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGcc                                                                                                             <  1:558649/150‑1 (MQ=255)
                                          ggTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCCCTACCGCACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCa                                                                                                            >  2:503609/1‑150 (MQ=255)
                                          ggTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCa                                                                                                            >  2:516973/1‑150 (MQ=255)
                                            gcTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGt                                                                                                          <  2:475780/149‑1 (MQ=255)
                                              tGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGTGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCAGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTc                                                                                                        <  2:910278/150‑1 (MQ=255)
                                              tGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGACACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTc                                                                                                        >  1:409186/1‑150 (MQ=255)
                                              tGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGACACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTc                                                                                                        >  1:1331445/1‑150 (MQ=255)
                                                   ggTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTAt                                                                                                   >  1:18365/1‑150 (MQ=255)
                                                         gCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATc                                                                                             >  1:440865/1‑150 (MQ=255)
                                                         gCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACAGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATc                                                                                             >  1:554788/1‑150 (MQ=255)
                                                           gTTAGCTATGTATCACTGCGCGCGCCCGGAACATCGCCATAAAATTAAAGGGCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCa                                                                                           <  1:885072/150‑1 (MQ=255)
                                                                 tATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCt                                                                                     <  2:35217/150‑1 (MQ=255)
                                                                     tATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGttt                                                                                 >  2:914742/1‑149 (MQ=255)
                                                                                   cccGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCtt                                                                   >  1:747439/1‑150 (MQ=255)
                                                                                    ccGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGCTCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTc                                                                  >  1:1135816/1‑150 (MQ=255)
                                                                                    ccGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGGCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGATCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTc                                                                  <  2:948736/150‑1 (MQ=255)
                                                                                          aaTCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGACTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCTCCGTc                                                            >  2:1046971/1‑150 (MQ=255)
                                                                                             cGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATg                                                         <  1:409144/150‑1 (MQ=255)
                                                                                                 aTAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCtg                                                     >  1:306597/1‑150 (MQ=255)
                                                                                                      aTTAAAGGTCTGATGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTc                                                <  1:383954/150‑1 (MQ=255)
                                                                                                         aaaGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGc                                             >  1:1295843/1‑150 (MQ=255)
                                                                                                         aaaGGTCTGCTGATTTCTGGCCTGAGCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGc                                             <  1:1087290/150‑1 (MQ=255)
                                                                                                          aaGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCg                                            <  1:1064267/150‑1 (MQ=255)
                                                                                                            ggTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCCCCGTCATGTCTGTGCTCGGCGTc                                          >  1:99752/1‑150 (MQ=255)
                                                                                                            ggTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTc                                          >  2:1120393/1‑150 (MQ=255)
                                                                                                             gTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCa                                         >  2:342203/1‑150 (MQ=255)
                                                                                                                tgctgATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGACCCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCa                                      >  1:687832/1‑150 (MQ=255)
                                                                                                                tgctgATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCa                                      >  1:178467/1‑150 (MQ=255)
                                                                                                                   tgATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCCCCGTCATGTCTGTGCTCGGCGTCACCATCg                                   >  1:1155106/1‑150 (MQ=255)
                                                                                                                    gATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGGTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCGCGCGGTCACCATCgg                                  >  2:829443/1‑150 (MQ=255)
                                                                                                                             ccTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGa                         <  2:1295843/150‑1 (MQ=255)
                                                                                                                                gATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACgg                      >  2:658053/1‑150 (MQ=255)
                                                                                                                                gATCGCCTGCGGCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACgg                      <  2:387206/150‑1 (MQ=255)
                                                                                                                                 aTCGCCTGCGTCGTTGGCGACACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGc                     <  2:1331445/150‑1 (MQ=255)
                                                                                                                                 aTCGCCTGCGTCGTTGGCGACACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGc                     <  2:409186/150‑1 (MQ=255)
                                                                                                                                   cGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCCCGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCCACCTCGGTAATACCGACGGCaa                   >  2:674661/1‑150 (MQ=255)
                                                                                                                                      cTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGGTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAatat                <  2:1111642/150‑1 (MQ=255)
                                                                                                                                       tGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATatc               >  2:127836/1‑150 (MQ=255)
                                                                                                                                             gtTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGAGCTCGGCGTCACCATCGGTAATACCGCCGGCAATATCATCGAc         >  1:859914/1‑150 (MQ=255)
                                                                                                                                                  cggcACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCgt    <  2:942309/150‑1 (MQ=255)
                                                                                                                                                    gcACTACCGCACCGCAGGAATTCCAGTTCCCGTTCGTAGCGCCAGATCTGTATGTCATCCACGCGCTGCTCACCGGCCGCGGCGTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCgtgg  <  1:686940/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGG  >  NC_000913/1700213‑1700510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑