Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 126,392 C→A 17.2% A233D (GCC→GAC) ‡ aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913126,3920CA17.2% 150.0 / 10.0 69A233D (GCC→GAC) ‡aceFpyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
Reads supporting (aligned to +/- strand):  ref base C (35/21);  new base A (6/7);  total (41/28)
Fisher's exact test for biased strand distribution p-value = 3.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCGGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGATCTTCGAAGTTGAAGGCG  >  NC_000913/126245‑126541
                                                                                                                                                   |                                                                                                                                                     
gcgcGGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCCGCTGGCGGGAAAGAAGAAACCGATCACGATATAGGCATAGACGAAGTTGAAGTTACAGAAGGGAAGGAGAAAGTGGGCGTCAAAGTTTCCg                                                                                                                                                     >  2:1258554/1‑150 (MQ=255)
     gCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGaa                                                                                                                                                >  2:950995/1‑150 (MQ=255)
        gCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGATGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAg                                                                                                                                             >  1:540743/1‑150 (MQ=255)
        gCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACAGAAGAGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAg                                                                                                                                             >  2:636601/1‑150 (MQ=255)
         cTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGGTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGt                                                                                                                                            >  2:860899/1‑150 (MQ=255)
          tCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTc                                                                                                                                           <  2:1283535/150‑1 (MQ=255)
              gCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGGAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTg                                                                                                                                       >  1:43634/1‑150 (MQ=255)
                 gCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATc                                                                                                                                    >  2:396619/1‑150 (MQ=255)
                    aaaCAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCAcc                                                                                                                                 <  2:775166/150‑1 (MQ=255)
                       cAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTa                                                                                                                              >  1:1245809/1‑150 (MQ=255)
                        aGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAg                                                                                                                             <  2:1104023/150‑1 (MQ=255)
                         ggAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGa                                                                                                                            >  1:1042608/1‑150 (MQ=255)
                              cagcTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCg                                                                                                                       >  1:1173800/1‑150 (MQ=255)
                                   ccGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACaaa                                                                                                                  >  2:262878/1‑150 (MQ=255)
                                    cGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAg                                                                                                                 <  1:406923/150‑1 (MQ=255)
                                      gCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGATGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCt                                                                                                               >  2:640604/1‑150 (MQ=255)
                                         gCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGAAAAAGCTTCt                                                                                                            >  2:818384/1‑150 (MQ=255)
                                            gCCCCTGCACCAGAGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATg                                                                                                         >  1:873101/1‑150 (MQ=255)
                                                     ccAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCCCCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCg                                                                                                >  2:888982/1‑150 (MQ=255)
                                                     ccAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCg                                                                                                >  1:929007/1‑150 (MQ=255)
                                                         cggcggGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACACAGCTTCTATGGAAGTTCCGgcgc                                                                                            >  1:279117/1‑150 (MQ=255)
                                                             tGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCggt                                                                                        >  2:202745/1‑148 (MQ=255)
                                                              ggCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGttt                                                                                       >  1:861220/1‑150 (MQ=255)
                                                                cGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGc                                                                                     >  2:1022325/1‑150 (MQ=255)
                                                                 gTGAAAGAAGTTAAAGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGTCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCa                                                                                    >  2:255248/1‑150 (MQ=255)
                                                                     aagacgTTAACGTTCCGGATATCGGCGGCGACGACGTTGAAGTGACTGAAGTCAGGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCCCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGcg                                                                                <  1:1286930/150‑1 (MQ=17)
                                                                        aagTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGATGCCGCTGAACAGTCACTGATCCCCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGATTGCAGGcgtcc                                                                             >  2:571892/1‑149 (MQ=255)
                                                                          gTTAACGTTCCGGATATCGGCTGCGACGAAGTTGAAGTGACCGAAGTGCTGGTGAAAGTTGGCGAAAAAGTTGACGCTGAACAGTCTCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTg                                                                           <  2:523074/150‑1 (MQ=11)
                                                                          gTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTg                                                                           >  2:1085856/1‑150 (MQ=255)
                                                                              aCGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAgg                                                                       <  1:756957/150‑1 (MQ=255)
                                                                               cGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGATGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGa                                                                      >  1:172137/1‑150 (MQ=255)
                                                                                  tCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACt                                                                   <  1:1062352/150‑1 (MQ=255)
                                                                                  tCCGGATATCGGCCGAGACGAAGTTGAAGTGACCTAACTGATGGTGAAAGTGGGCGACAAAGTTGAAGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACt                                                                   <  1:592361/150‑1 (MQ=17)
                                                                                   ccGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTg                                                                  <  1:743189/150‑1 (MQ=255)
                                                                                    cGGATATCGGCGGTGACGAAGTAGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCAGTTTGCAGGCGTCGTGAAGGAACTGa                                                                 >  1:854564/1‑150 (MQ=255)
                                                                                     ggATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGACGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGATGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTgta                                                                >  2:216093/1‑148 (MQ=255)
                                                                                     ggATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGGGAAAGGGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGaa                                                                <  2:861468/150‑1 (MQ=255)
                                                                                       atatCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAg                                                              <  1:881100/150‑1 (MQ=255)
                                                                                       atatCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAg                                                              <  2:481787/150‑1 (MQ=255)
                                                                                            ggcggTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAAc                                                         >  1:1121785/1‑150 (MQ=255)
                                                                                              cggTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGt                                                       >  1:1050607/1‑150 (MQ=255)
                                                                                                        gTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGATGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATaaa                                             >  1:545409/1‑150 (MQ=14)
                                                                                                          tGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGt                                           >  1:267236/1‑150 (MQ=255)
                                                                                                            aaGTGACTGAATTGATGGTGAAAGTGGGCGAAAAAGTTGACGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGa                                         <  1:633056/150‑1 (MQ=255)
                                                                                                            aaGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGa                                         <  2:545409/150‑1 (MQ=14)
                                                                                                                gACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAAc                                     >  2:270254/1‑150 (MQ=255)
                                                                                                                    gAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGc                                 >  2:156743/1‑150 (MQ=255)
                                                                                                                    gAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGc                                 <  2:685101/150‑1 (MQ=255)
                                                                                                                    gAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGc                                 >  1:1100873/1‑150 (MQ=255)
                                                                                                                         gATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCt                            >  2:1094404/1‑150 (MQ=255)
                                                                                                                            ggTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCTCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCg                                                  <  1:899928/125‑1 (MQ=17)
                                                                                                                            ggTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCg                                                  >  2:899928/1‑125 (MQ=17)
                                                                                                                             gTGAAAGTGGGCGAGAAAGTTGCCGCTGAACAGTCACTGATCACCGTATAAGGCGACAACTCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATATAGTGAAAACTGGCTCGCTGAtt                        <  1:992254/150‑1 (MQ=255)
                                                                                                                             gTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGCTCACCGTAGCAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGATGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGAtt                        >  1:661098/1‑150 (MQ=255)
                                                                                                                             gTGAAAGTGGGCGACAAAGTTGACGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGAtt                        <  1:423705/150‑1 (MQ=255)
                                                                                                                              tGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTa                       <  1:262878/150‑1 (MQ=255)
                                                                                                                               gAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTAt                      <  1:154810/150‑1 (MQ=255)
                                                                                                                                 aagttcgCGACATAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGa                    <  1:1023264/144‑1 (MQ=255)
                                                                                                                                 aaGTGGGCGACAAAGTTGCCGCTGAACAGTCACTTATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGa                    <  1:636601/150‑1 (MQ=255)
                                                                                                                                 aaGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGa                    >  2:815503/1‑150 (MQ=255)
                                                                                                                                   gttggCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCAGGCGTCGTGAAGGAACTGGAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGATc                  >  2:430247/4‑150 (MQ=17)
                                                                                                                                   gttggCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGCAAGTTCCGGCTCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGAGATAAAGTGAAAACTGGCTCGCTGATTATGATc                  >  2:904869/4‑150 (MQ=17)
                                                                                                                                       gCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCCACGTTGGCGATAAAGTGAAAACTGGCGAGCTGATTATGATCTTCg              >  1:186655/1‑150 (MQ=255)
                                                                                                                                        cGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGATCTTCGa             <  1:950995/150‑1 (MQ=255)
                                                                                                                                          ataaaGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAACGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGGCGTGAAGGAACTGAAAGTCAACGTTGGAGAGAAAGTGAAAACGGGCTCGCTGATTATGATCTTCGAAg           >  1:277112/3‑150 (MQ=17)
                                                                                                                                           taacgTTGACGCTGAACAGTCGCTGATCACCGTAGACGGCGACAAGGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGGCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGATCTTCGAAGt          <  2:277112/146‑1 (MQ=18)
                                                                                                                                           cAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGATCTTCGAAGt          <  2:475254/150‑1 (MQ=255)
                                                                                                                                           cAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGATCTTCGAAGt          <  2:929007/150‑1 (MQ=255)
                                                                                                                                             aaGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGACGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGATCTTCGAAGTTg        <  2:1245809/150‑1 (MQ=255)
                                                                                                                                                   ccGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCGGATTATGATCTTCGAAGTTGACGgcg  >  1:856146/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCGCGGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGCGTCGTGAAGGAACTGAAAGTCAACGTTGGCGATAAAGTGAAAACTGGCTCGCTGATTATGATCTTCGAAGTTGAAGGCG  >  NC_000913/126245‑126541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: