Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,990,086 T→C 5.6% C136R (TGT→CGT)  tyrP → tyrosine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,990,0860TC 5.6% 208.7 / 3.4 71C136R (TGT→CGT) tyrPtyrosine transporter
Reads supporting (aligned to +/- strand):  ref base T (35/32);  new base C (2/2);  total (37/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

GTATGATGTTCTTAATGTATGCTCTGACTGCGGCATACATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACAAAGTGAATCTTTTAACCCTGCCGTTGCAACAGGGGCTGGCTCTGTC  >  NC_000913/1989937‑1990234
                                                                                                                                                     |                                                                                                                                                    
gTATGATGTTCTTAATGTATGCTCTGACTGCGGCATACATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCAGGTGGCGTGGttt                                                                                                                                                      >  1:862160/1‑150 (MQ=255)
gTATGATGTTCTTAATGTATGCTCTGACTGCGGCATACATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTAGTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGttt                                                                                                                                                      >  2:368971/1‑150 (MQ=255)
     ttgTTCTTATTGTATGCTCTGACTGCGTCATACATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTc                                                                                                                                                 <  1:998797/149‑1 (MQ=255)
         tCTTAATGTATGCTCTGACTGCGGCATACATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGGTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGaa                                                                                                                                             >  2:1130712/1‑150 (MQ=255)
           ttAATGTATGCTCTGACTGCGGCATACATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACt                                                                                                                                           <  1:770241/150‑1 (MQ=255)
           ttAATGTATGCTCTGACTGCGGCATACATCAGCGGTGCCGGTGAATTGTTGGCCTACAGCATCAGAGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCCCTTGTTCACTTTTGTTTCCGGTGGCGTGGTTTGTGTCGGaaat                                                                                                                                           >  1:208486/1‑148 (MQ=255)
           gtAATGTATGCGCTGCCTGCGGCATACATCAGCGGTGCCGGGGAATTGTTGGCCTCCAGCATCCGCGACTGGACAGGTATTTCTATGGCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACt                                                                                                                                           <  1:1040541/149‑1 (MQ=255)
            tAATGTATGCTCTGACTGCGGCATACATCAGCGGTGACGGTGAATTGTTGGCCTCCAGAATCAGCGACTGGCCAGGTATTTCTATGTCGGCAACCGCTGGCGGGCTGTTGTTCACTTTTGTTGCCGGTGTCGTGGTTTGTGTCGGAACtt                                                                                                                                          >  1:382366/1‑150 (MQ=255)
                 tATGCTCTGACTGCGGCATACATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCGGGCGTGCTGTTGTTCACGTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTg                                                                                                                                     >  2:140252/1‑150 (MQ=255)
                     ctctGACTGCGGCATACATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCg                                                                                                                                 >  2:602443/1‑150 (MQ=255)
                       ctGACTGCGGCATACATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGat                                                                                                                               <  1:1030349/150‑1 (MQ=255)
                       ctGACTGCGGCATACATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGat                                                                                                                               >  1:703787/1‑150 (MQ=255)
                       ctGACTGCGGCATACATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGat                                                                                                                               >  2:1228263/1‑150 (MQ=255)
                        tGACTGCGGCATACATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGTTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGatt                                                                                                                              <  1:787238/150‑1 (MQ=255)
                          aCTGCGGCATACATCAGCGGGGCCGGGGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTAGTGCTATGTCGGCAACCGCTGGCGTGCTGTTGTTGACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGattta                                                                                                                            <  1:220937/150‑1 (MQ=255)
                              cGGCATACATCAGCGGGGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGAtttattta                                                                                                                        <  1:946248/150‑1 (MQ=255)
                                gCATACATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAAc                                                                                                                      >  2:1348495/1‑150 (MQ=255)
                                gCATACATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAAc                                                                                                                      >  2:467861/1‑150 (MQ=255)
                                gCATACATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAAc                                                                                                                      <  1:1062433/150‑1 (MQ=255)
                                gCATACATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCCTGCTGTTGTTCACTTTTGTTGCCGGTGGCCTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAAc                                                                                                                      >  2:646039/1‑150 (MQ=255)
                                     cATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGttt                                                                                                                 >  2:44459/1‑150 (MQ=255)
                                     cATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGttt                                                                                                                 >  2:765707/1‑150 (MQ=255)
                                     cATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGttt                                                                                                                 >  2:962116/1‑150 (MQ=255)
                                       tCAGCGGTGCCGGGGACTTGTGGGCCGCCAGCATCAGCGACTGGACATGGATTTCTCTGTCGGCAACCGCTGGCGTGCTGTTCTTCACTTTTGTTGCCGTTGGCGTGGTTTGTGTCGGAACTTCACTGGTAGATTTATTTAACCGTTTTc                                                                                                               <  1:62221/150‑1 (MQ=255)
                                        cAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTTTTTATCCGTTTTCt                                                                                                              >  1:303748/1‑150 (MQ=255)
                                        cAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTTACCGTTTTCt                                                                                                              >  1:308680/1‑150 (MQ=255)
                                          gCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTTGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGt                                                                                                            <  2:983937/150‑1 (MQ=255)
                                            ggTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGGAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTc                                                                                                          <  2:180899/150‑1 (MQ=255)
                                                ccGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAgcg                                                                                                      <  2:383043/150‑1 (MQ=255)
                                                     gcaTTGTTGCCCGCCAGCATCTGCGACTGGACAGGTATTTCTATGTCCGCAACCGCTGGCGTGATTTTTTTCACTTTTTTTGCCTGTGTCGTGGTTTGTGTCGGAAGTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGAGCCAAg                                                                                                 <  1:865389/148‑1 (MQ=255)
                                                       tttgatgGCCTCCAGCATCAGCGTCTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGat                                                                                               <  1:982565/149‑1 (MQ=255)
                                                         tgttgGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGatta                                                                                             >  1:126722/1‑150 (MQ=255)
                                                              gCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTAttttt                                                                                        >  1:1200261/1‑150 (MQ=255)
                                                              gCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTAttttt                                                                                        >  1:1205670/1‑150 (MQ=255)
                                                               ccTCCAGCATCAGCGACTGGTCAGGTATTTCTTTGTCGGCAACCGGTGGCGTGCTGTTGGTCTCTTTTGTTGCCGGTGGCGTGGTTTGTGGCGGATCTTCAGTTGGCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTc                                                                                       <  1:1258389/150‑1 (MQ=255)
                                                               ccTCCAGCATCAGCGACTGGACAGGTTTTTCTTTGTCGGCATCCGCTGGCGTGCTGGTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGTTTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTc                                                                                       <  1:1351771/150‑1 (MQ=255)
                                                               ccTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTGCAGCGCCAAGATTATTTTTc                                                                                       <  1:790308/150‑1 (MQ=255)
                                                                  ccAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCtgg                                                                                    <  2:1025893/150‑1 (MQ=255)
                                                                      cATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGGTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTggtggt                                                                                <  2:372271/150‑1 (MQ=255)
                                                                       aTCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCCTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTa                                                                               >  2:298/1‑150 (MQ=255)
                                                                           gCGACTGGCCAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTTCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGc                                                                           <  1:179171/150‑1 (MQ=255)
                                                                            cGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCt                                                                          <  2:1241934/150‑1 (MQ=255)
                                                                                  gACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGtact                                                                    >  2:732325/1‑150 (MQ=255)
                                                                                  gACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAAATAAACAGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGATGGtact                                                                    >  2:873119/1‑150 (MQ=255)
                                                                                    cAGGTATTTCTAGGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTTCCGTTGTCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTTTTTAACCGTTTTGTGGTCTGCGCGAAGCTGATTGTTCTGGTGGTAATGCTGGtagtac                                                                  >  1:197092/1‑150 (MQ=255)
                                                                                     aGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTactact                                                                 >  1:208665/1‑150 (MQ=255)
                                                                                      ggTATTTCTATGTGGGCAACCGCTGGCGTGCCGTTGTTCACTTTTGTTGCCGGTTTCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTActg                                                                <  2:208486/150‑1 (MQ=255)
                                                                                      ggTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTTATGCTGGTACTActg                                                                >  1:1249734/1‑150 (MQ=255)
                                                                                        tATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTActgct                                                              <  1:969024/150‑1 (MQ=255)
                                                                                           ttCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGcc                                                           >  2:384124/1‑150 (MQ=255)
                                                                                           ttCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGcc                                                           <  1:368971/150‑1 (MQ=255)
                                                                                              tATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCa                                                        >  2:1024616/1‑150 (MQ=255)
                                                                                                      cAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTcaca                                                <  2:1275340/150‑1 (MQ=255)
                                                                                                      cAACCGCTGGCGTGCTGTTGTTCAC‑TTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACaa                                               >  1:494300/1‑150 (MQ=255)
                                                                                                        aCCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTTATGCTGGTACTACTGCTGCCGCATATTCACaaa                                              >  2:177840/1‑150 (MQ=255)
                                                                                                        aCCGCTGGCGGGCGGTTGTTCACTTTAGTTGCCGGTGGCGTGGTGTGGGTCGGAACTTCACTGGGCGATTTATATAACCGTTTTCTGTTCAGCGCCAAGACTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACaaa                                              <  1:629539/150‑1 (MQ=255)
                                                                                                          cGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACAAAGt                                            >  1:871470/1‑150 (MQ=255)
                                                                                                          cGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACAAAGt                                            >  1:375836/1‑150 (MQ=255)
                                                                                                           gCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACAAAGTg                                           >  1:2884/1‑150 (MQ=255)
                                                                                                                cGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTACCGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGATGCCGCATATTCACAAAGTGAATCt                                      >  1:122924/1‑150 (MQ=255)
                                                                                                                 gTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTCGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACAAAGTGAATCtt                                     >  2:1278847/1‑150 (MQ=255)
                                                                                                                 gTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTCGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACAAAGTGAATCtt                                     >  2:1284071/1‑150 (MQ=255)
                                                                                                                  tGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACAAAGTGAATCttt                                    >  1:535421/1‑150 (MQ=255)
                                                                                                                   gCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACAAAGTGAATCtttt                                   <  1:1205953/150‑1 (MQ=255)
                                                                                                                   gCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACAAAGTGAATCtttt                                   >  1:800824/1‑150 (MQ=255)
                                                                                                                          ttCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACAAAGTGAATCTTTTAACCCTg                            <  2:703787/150‑1 (MQ=255)
                                                                                                                                 ttgttgCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACAAAGTGAATCTTTTAACCCTGCCGTTGc                     <  2:603403/150‑1 (MQ=255)
                                                                                                                                 ttgttgCCGGTGGCGTGGTTTGTGTAGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACAAAGTGAATCTTTTAACCCTGCCGTTGc                     <  2:1013230/150‑1 (MQ=255)
                                                                                                                                   gttgCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGATGGTAATACTGCTGCCGCATATTCACAAAGTGAATCTTTTAACCCTGCCGTTGCaa                   >  2:1018569/1‑150 (MQ=255)
                                                                                                                                    ttgCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCTCATATTCACAAAGTGAATCTTTTAACCCTGCCGTTGCAAc                  <  1:1008181/150‑1 (MQ=255)
                                                                                                                                          gTGGCGTGGTTTGTGGCGGAACTTCACTGGTGGATTTCTTTAACCGTTTTCTGTTCAGCGTCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACAAAGTGAATCTTTTAACCCTGCCGTTGCAACAGGggc            <  2:1200220/150‑1 (MQ=255)
                                                                                                                                            ggCGTGGTTCGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACAAAGTGAATCTTTTAACCCTGCCGTTGCAACAGGggctg          <  1:1278847/150‑1 (MQ=255)
                                                                                                                                            ggCGTGGTTCGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACAAAGTGAATCTTTTAACCCTGCCGTTGCAACAGGggctg          <  1:1284071/150‑1 (MQ=255)
                                                                                                                                                   gttGGGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACAAAGTGAATCTTTTAACCCTGCCGTTGCAACAGGGGCTGGCtctgt   <  2:476682/149‑1 (MQ=255)
                                                                                                                                                    ttGTGTCGGAACTTCACTGGGCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACAAAGTGAATCTTTTAACCCTGCCGTTGCAACAGGGGCTGGCtctgtc  <  1:646039/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTATGATGTTCTTAATGTATGCTCTGACTGCGGCATACATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACAAAGTGAATCTTTTAACCCTGCCGTTGCAACAGGGGCTGGCTCTGTC  >  NC_000913/1989937‑1990234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑