Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,019,460 G→A 100% V359I (GTC→ATC)  fliK → flagellar hook‑length control protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,019,4600GA100.0% 240.1 / NA 76V359I (GTC→ATC) fliKflagellar hook‑length control protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/37);  total (39/37)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAA  >  NC_000913/2019318‑2019606
                                                                                                                                              |                                                                                                                                                  
tGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCttt                                                                                                                                             >  1:348404/1‑150 (MQ=255)
 ggCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGGCGATACGCTTCCGGTTCCCATCTCTTTa                                                                                                                                            >  2:472328/1‑150 (MQ=255)
 ggCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTa                                                                                                                                            >  1:1228147/1‑150 (MQ=255)
  gCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTTGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTAc                                                                                                                                           >  2:788701/1‑150 (MQ=255)
    aTTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACATCTGGCGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAg                                                                                                                                        >  1:594430/1‑150 (MQ=255)
       cAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAggg                                                                                                                                      >  1:1131392/1‑150 (MQ=255)
       cAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAggg                                                                                                                                      <  1:740913/150‑1 (MQ=255)
        aGTTAGGGCAAAGCAACATCAGTGGCGCAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCACCGGACCGCAACCCATGACACTCTGTCTGGGGAATACGACGATACGCTTCCGGTTACAATCTCTTTACAAGTGc                                                                                                                                     >  2:800760/1‑150 (MQ=255)
              ggCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTaa                                                                                                                               >  1:1094036/1‑150 (MQ=255)
              ggCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTaa                                                                                                                               >  2:945635/1‑150 (MQ=255)
              ggCAAAGCAACAGCAGTGGCGACAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCACCGCACAGCACACGATGACCCGCTGGCGGGGGACGCCGCCGCTACGCTGCCGGTGGCCAGAGCTTTACACGGGCGTGTaa                                                                                                                               >  2:1224815/1‑150 (MQ=255)
                cAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACa                                                                                                                             <  1:922360/150‑1 (MQ=255)
                cAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACa                                                                                                                             <  2:353427/150‑1 (MQ=255)
                 aaaGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAg                                                                                                                            <  2:795329/150‑1 (MQ=255)
                 aaaGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAg                                                                                                                            <  2:1014167/150‑1 (MQ=255)
                 aaaGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAg                                                                                                                            <  2:1348424/150‑1 (MQ=255)
                  aaGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGTTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAgg                                                                                                                           <  1:398387/150‑1 (MQ=255)
                  aaGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGAAACTGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGAGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAgg                                                                                                                           <  2:223104/150‑1 (MQ=255)
                  aaGCAACATCAGTGGCGAAAGCGTTAGTGGTCAGCAGCAGGCGGCTTCCCCGGAACCGCAACGCCAACGCACAGCAAACCATCAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACACGGGCGTGTAACAgg                                                                                                                           <  2:191485/150‑1 (MQ=255)
                    gCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCa                                                                                                                         <  1:400348/150‑1 (MQ=255)
                        cATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAg                                                                                                                     <  1:108794/150‑1 (MQ=255)
                            aGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCGTTACAAGGGCGTGTACCAGGCAACAgcggc                                                                                                                 >  1:630769/1‑150 (MQ=255)
                                gCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACATCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGTGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTg                                                                                                             <  1:40365/150‑1 (MQ=255)
                                   aaaGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGata                                                                                                          <  2:1034621/150‑1 (MQ=255)
                                   aaaGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGata                                                                                                          >  1:688655/1‑150 (MQ=255)
                                      gCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTCTAACAGGCAACAGCGGCGTTGATAttt                                                                                                       >  2:33381/1‑150 (MQ=255)
                                      gCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCAGGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAttt                                                                                                       <  1:74369/150‑1 (MQ=255)
                                           aGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGcc                                                                                                  <  2:170001/150‑1 (MQ=255)
                                                  agcagcagGCCGCGTCCCAGCAACAGCCAAGCCAACGCACAGCCCACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCGGGTTCCCATCTCTTTAGAAGGTCGTGTAACAGGCAACAGCGGCGGAGATATTTTCGCATAACGTc                                                                                           >  1:84488/1‑150 (MQ=255)
                                                   gcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCa                                                                                          >  1:422098/1‑150 (MQ=255)
                                                    cagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCag                                                                                         <  2:1230876/150‑1 (MQ=255)
                                                     agcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAACCCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGTCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCaga                                                                                        <  2:816312/150‑1 (MQ=255)
                                                      gcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagag                                                                                       <  2:21841/150‑1 (MQ=255)
                                                         ggCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTa                                                                                    >  1:630753/1‑150 (MQ=255)
                                                          gCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAg                                                                                   >  1:173616/1‑150 (MQ=255)
                                                              cTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGACCCTCTGGCGGGGGACGACGACGCTACGCTTCCGGTTCCCATCTCTGTACAAGGGCGTGTAACAGGCCACAGCGGCGTTGAGATTTTCGCCTAACGTCAGCGGTCGCAcc                                                                               >  1:954095/1‑150 (MQ=255)
                                                              cTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTTACGTCAGAGGTAGCAcc                                                                               >  1:1281541/1‑150 (MQ=255)
                                                                     gcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATcc                                                                        >  1:893523/1‑150 (MQ=255)
                                                                     gcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATcc                                                                        >  2:1050562/1‑150 (MQ=255)
                                                                      caacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCcg                                                                       >  2:1085940/1‑150 (MQ=255)
                                                                       aacagcaaAGCCAACGCACAGCAAACCATGATCCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACACGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCcgc                                                                      <  1:201082/150‑1 (MQ=255)
                                                                        acagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCcgcg                                                                     >  1:329897/1‑150 (MQ=255)
                                                                        acagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCcgcg                                                                     >  2:831984/1‑150 (MQ=255)
                                                                           gcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCt                                                                  >  2:1256724/1‑150 (MQ=255)
                                                                                  cAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGCCGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTcccc                                                           >  1:359456/1‑150 (MQ=255)
                                                                                         caGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACTGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTttgt                                                    >  2:903692/1‑150 (MQ=255)
                                                                                           gCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACATGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTttgttg                                                  <  1:146510/150‑1 (MQ=255)
                                                                                           gCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTttgttg                                                  <  2:681483/150‑1 (MQ=255)
                                                                                           gCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTttgttg                                                  <  2:688655/150‑1 (MQ=255)
                                                                                              aaCCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTgcgc                                               <  2:519771/150‑1 (MQ=255)
                                                                                              aaCCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTgcgc                                               >  1:1206874/1‑150 (MQ=255)
                                                                                                 cATGAACCTCTGGCGGGGGAAGACGACGATCCGCTTCCGGGTCCCATCTCTTTACCAGGGCGTGTAACAGGCAACAGCGGCGGTGATATTTTCGCCGAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCa                                            <  1:832703/150‑1 (MQ=255)
                                                                                                 cATGAACCTCTGGCAGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCa                                            >  1:1406/1‑150 (MQ=255)
                                                                                                     aaCGTCTGTCGGGGGAATACGACGATCCGCTTCCGGTTCCAAACTCTTAACAAGGTCGAGAAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCCTTTCCCCGCTTTGTTGCGCTCAacac                                        <  2:710358/150‑1 (MQ=255)
                                                                                                      aCCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTATACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACg                                       <  1:418639/150‑1 (MQ=255)
                                                                                                        ctctGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCa                                     <  1:376887/150‑1 (MQ=255)
                                                                                                          ctGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAgg                                   <  1:729002/150‑1 (MQ=255)
                                                                                                             gCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATa                                >  2:174019/1‑150 (MQ=255)
                                                                                                             gCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATa                                >  1:1143439/1‑150 (MQ=255)
                                                                                                             gCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATa                                <  2:474186/150‑1 (MQ=255)
                                                                                                                ggggAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAAtt                             >  2:786519/1‑150 (MQ=255)
                                                                                                                  ggAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGTCGCAGGATAATTAg                           >  2:849838/1‑150 (MQ=255)
                                                                                                                  ggAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCATAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGAATGATAATTAg                           >  2:418804/1‑150 (MQ=255)
                                                                                                                   gAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGc                          >  1:1294718/1‑150 (MQ=255)
                                                                                                                   gAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGc                          <  2:740203/150‑1 (MQ=255)
                                                                                                                    aaGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGTGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCTCCTAACGTCAGAGGTAGAACCGTAATCCACGACTTTTCACAGCTTTGTTGCGCTCAAGACTCAGGATAAATAGcc                         >  1:1234857/1‑150 (MQ=255)
                                                                                                                     aGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCg                        <  1:1235054/150‑1 (MQ=255)
                                                                                                                              tACTCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGt               <  1:759381/150‑1 (MQ=255)
                                                                                                                              tACGCTTCCGGTTCCCAGCTCTTTACAAGGGCGGGTAACAGGCAACCGCGGCGTTGATATTTTCGCCTAACGTAAGAGGTATCACCGTAATCCGCGTCTTTTCCGCGGTTTGTTGCGCTCAAGACGCAGTATAATTAGCCGATAAGCAGt               <  1:1224815/150‑1 (MQ=255)
                                                                                                                                cGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAg             <  2:173616/150‑1 (MQ=255)
                                                                                                                                cGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAg             >  2:413905/1‑150 (MQ=255)
                                                                                                                                 gCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATTATTAGCCGATAAGCAGTAGc            <  2:448847/150‑1 (MQ=255)
                                                                                                                                    tCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGac         <  2:422098/150‑1 (MQ=255)
                                                                                                                                    tCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGac         <  2:893523/150‑1 (MQ=255)
                                                                                                                                       ggTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGAcaca      >  2:394269/1‑150 (MQ=255)
                                                                                                                                           cccATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGaa  >  1:785050/1‑150 (MQ=255)
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TGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAA  >  NC_000913/2019318‑2019606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: