Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,363,453 C→T 6.2% A61T (GCC→ACC) ‡ yfaY ← inactive PncC family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,363,4530CT 6.2% 321.5 / 6.1 99A61T (GCC→ACC) ‡yfaYinactive PncC family protein
Reads supporting (aligned to +/- strand):  ref base C (40/52);  new base T (3/3);  total (44/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01

Only 100 of 101 total aligned reads displayed.

TACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGCCAGCCAGGCAGCGTTAGTGTCAACGATTTGCCCGTGTAACACTTCAT  >  NC_000913/2363304‑2363602
                                                                                                                                                     |                                                                                                                                                     
gctatttCCACGAACGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACACGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGGCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGc                                                                                                                                                       >  1:333007/7‑150 (MQ=255)
  cTCGTCCACGTTCGTGGAAATAGCGTTCAATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCTGCGt                                                                                                                                                     >  1:468053/1‑150 (MQ=255)
   tCGGCCACGATCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTg                                                                                                                                                    <  1:1149470/150‑1 (MQ=255)
    cGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGCGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGGGGCGAGTGCGCTTAAATCATCGCGGGTCGGTCCCTGCCCGCCGTTAACGATCGGCACAGCGGGGTgg                                                                                                                                                   >  2:192841/1‑149 (MQ=255)
    cGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGc                                                                                                                                                   <  1:568254/150‑1 (MQ=255)
       ccACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCATCCCGCCGTTAACGATCAGCACATCGGCGTGCTGa                                                                                                                                                >  2:400698/1‑150 (MQ=255)
       ccACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACAAGGCCTTCACCTTATGCTGTCGCAGCGGCGAGTGCGATTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGa                                                                                                                                                >  2:395251/1‑150 (MQ=255)
        cACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGAc                                                                                                                                               >  2:1300843/1‑150 (MQ=255)
          cGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCGGTTAACGATCAGCACATCGGCGTGCTGagtt                                                                                                                                             >  2:64514/1‑147 (MQ=255)
          cGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACGCCGATGTGCTGatcg                                                                                                                                             <  1:886011/150‑4 (MQ=255)
          cGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACGCCGATGTGCTGatcg                                                                                                                                             <  1:1271292/150‑4 (MQ=255)
           gTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCAACAGGACTTCACCTTTTGCTGTCGCAGCGGAGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTAAACGATCAGCACGCCGATGTGCTGatcgt                                                                                                                                            <  2:124829/150‑5 (MQ=255)
              cGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCCGCACATCGGCGTGCTGACTACGtt                                                                                                                                         >  1:673936/1‑150 (MQ=255)
                tGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTcg                                                                                                                                       <  1:597890/150‑1 (MQ=255)
                 ggAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTcgc                                                                                                                                      <  1:239023/150‑1 (MQ=255)
                  gAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTcgcg                                                                                                                                     <  1:19900/150‑1 (MQ=255)
                      tAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGACCGTCCCAGCCCTCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCgaaga                                                                                                                                 >  1:775091/1‑150 (MQ=255)
                       aGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCgaagaa                                                                                                                                <  1:1044687/150‑1 (MQ=255)
                       aGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCgaagaa                                                                                                                                >  1:1095269/1‑150 (MQ=255)
                       aGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCgaagaa                                                                                                                                <  1:689347/150‑1 (MQ=255)
                          gTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATgg                                                                                                                             >  2:449533/1‑150 (MQ=255)
                          gTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATgg                                                                                                                             <  1:727172/150‑1 (MQ=255)
                          gTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATgg                                                                                                                             >  1:285968/1‑150 (MQ=255)
                           ttCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGt                                                                                                                            >  1:368301/1‑150 (MQ=255)
                            tCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCTGCGAGTGCGCTTAAATCATCGCTGGTAGGTCCCAGCCCGCCGTTAACGATCATCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTg                                                                                                                           <  1:348520/150‑1 (MQ=255)
                            tCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTg                                                                                                                           >  1:1300934/1‑150 (MQ=255)
                             ccATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTgg                                                                                                                          >  2:547017/1‑149 (MQ=255)
                             ccATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGa                                                                                                                          >  2:317577/1‑150 (MQ=255)
                              cATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGAc                                                                                                                         >  2:281401/1‑150 (MQ=255)
                               aTCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACt                                                                                                                        <  2:1027187/150‑1 (MQ=255)
                                   ctTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGACCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGt                                                                                                                    >  1:443134/1‑150 (MQ=255)
                                       gAGCCAGGCTTCATGCAGCACCTTGCCTTCACCTTTTTCTGTAGCAGGGTCGAGTGCGCTTAAATCATCGCTGGTCGTTACCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGtcatc                                                                                                                <  1:81099/150‑1 (MQ=255)
                                       gAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGGCGCAGCGGCGAGTGCGCTTAACTCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGtcatc                                                                                                                <  1:145870/150‑1 (MQ=255)
                                        cgCCAGGCTGCATGCAGCACCAGGCCTTCACCTTTTGCGGGCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGGCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCGGACTACGTTCGCGAAGAATGGGGACTAAGtcatca                                                                                                               <  1:519401/149‑1 (MQ=255)
                                         gCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCaa                                                                                                              <  2:16608/150‑1 (MQ=255)
                                          ccAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAg                                                                                                             >  2:699170/1‑150 (MQ=255)
                                          ccAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACCATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAg                                                                                                             >  1:392441/1‑150 (MQ=255)
                                           cAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAgg                                                                                                            <  1:63274/150‑1 (MQ=255)
                                           cAGGCTTCATGCAGCACCAGGACTTCACCTTTTGCTGTCGCAGCGGCGAGAGCGCGTAAATCATCGCAGGTCGGTCCCACCCCGCCGTAAACGATCAGCACATCGGCGTGCAGACTACCTTCGCGAAGAATGGTGACTAAGTCATCAAgc                                                                                                            >  1:684015/1‑149 (MQ=255)
                                               cTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTAt                                                                                                        >  1:898653/1‑150 (MQ=255)
                                                            cAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTAtt                                                                                           <  2:559311/150‑1 (MQ=255)
                                                                 cTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTgcggc                                                                                      >  1:1011683/1‑150 (MQ=255)
                                                                 cTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTgcggc                                                                                      >  1:1040170/1‑150 (MQ=255)
                                                                 cTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCGTAAATCATCGCAGGTCGGTCCCAGCCCGCCGTTAACGATCAGCTCATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTgcggc                                                                                      <  1:552351/150‑1 (MQ=255)
                                                                    cACCTTTTGTTGTCGCAGCGGCGATTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACGAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCgag                                                                                   >  2:178553/1‑150 (MQ=255)
                                                                    cACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCgag                                                                                   <  2:268237/150‑1 (MQ=255)
                                                                    cACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCgag                                                                                   <  1:1061345/150‑1 (MQ=255)
                                                                      ccTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGACTGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGAt                                                                                 <  1:494559/150‑1 (MQ=255)
                                                                      ccTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGAt                                                                                 >  1:565435/1‑150 (MQ=255)
                                                                        ttttGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATaa                                                                               >  1:538468/1‑150 (MQ=255)
                                                                         tttGCTGGCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAAt                                                                              <  1:1140378/150‑1 (MQ=255)
                                                                          ttGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGGCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGATCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATg                                                                             <  2:778924/150‑1 (MQ=255)
                                                                           tGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATgg                                                                            >  1:749187/1‑150 (MQ=255)
                                                                           tGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATgg                                                                            <  2:884266/150‑1 (MQ=255)
                                                                           tGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATgg                                                                            <  2:436553/150‑1 (MQ=255)
                                                                            gCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGc                                                                           <  2:1180459/150‑1 (MQ=255)
                                                                             cTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCa                                                                          <  1:281401/150‑1 (MQ=255)
                                                                               gTCGCTGCGGCGAGTGCGGTTAAATCATCGCTGGTCGGTCCCAGCCCGCAGTTACCGATCAGCAGATCGGCGGGCTGCCTACGGTCGCGAAGGATGGGGACTAAGTCATCAAGGTGTTCCCCCACCGGATTGCGGCGAGATAATGGCAAc                                                                        >  2:203493/1‑150 (MQ=255)
                                                                                tCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAAcc                                                                       <  1:923625/150‑1 (MQ=255)
                                                                                 cGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAAccc                                                                      >  2:95256/1‑150 (MQ=255)
                                                                                  gCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTCCGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAAcccc                                                                     >  1:1265928/1‑150 (MQ=255)
                                                                                  gCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAATAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAAcccc                                                                     <  2:333007/150‑1 (MQ=255)
                                                                                     gcggcgAGTGCGCTTAAATCATCGCTGGTCGGGCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCtga                                                                  <  2:406333/150‑1 (MQ=255)
                                                                                      cggcgAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCtgat                                                                 <  1:1022054/150‑1 (MQ=255)
                                                                                       ggcgAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCtgatg                                                                <  2:751879/150‑1 (MQ=255)
                                                                                         cgAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATgaa                                                              <  1:698579/150‑1 (MQ=255)
                                                                                           aGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATgaaag                                                            <  2:1064739/150‑1 (MQ=255)
                                                                                           aGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATgaaag                                                            <  1:292346/150‑1 (MQ=255)
                                                                                             tGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATgaaagaa                                                          >  1:1019985/1‑150 (MQ=255)
                                                                                              gcgcTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATgaaagaaa                                                         <  1:985909/150‑1 (MQ=255)
                                                                                              gcgcTTAAATCATCGCTAGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATgaaagaaa                                                         >  2:1204825/1‑150 (MQ=255)
                                                                                               cgcTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGTTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGaaaa                                                        <  1:942747/150‑1 (MQ=255)
                                                                                               cgcTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGaaaa                                                        >  1:451589/1‑150 (MQ=255)
                                                                                               cgcTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGaaaa                                                        <  1:1295482/150‑1 (MQ=255)
                                                                                               cgcTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGaaaa                                                        <  1:155449/150‑1 (MQ=255)
                                                                                                 cTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCACCCCCTGATGAAAGaaaaaa                                                      >  1:154716/1‑150 (MQ=255)
                                                                                                 cTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGaaaaaa                                                      >  2:1256049/1‑150 (MQ=255)
                                                                                                 cTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCAAAACCGCGTTCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATAAAGGTTATCCCCAAACGTATTGCGGACAGATAATGGCAACCCCTCATGAAAGaaaaaa                                                      >  1:1112751/1‑150 (MQ=255)
                                                                                                  ttAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGagaaaaa                                                     <  1:956736/150‑2 (MQ=255)
                                                                                                    aaaTCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCg                                                   <  1:449533/150‑1 (MQ=255)
                                                                                                      atcatcGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCggg                                                 <  1:699170/150‑2 (MQ=255)
                                                                                                       tcatcGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGgcc                                                <  1:896596/150‑1 (MQ=255)
                                                                                                         atcGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACAGCGGCGTGCTGACTACGTTCGCTAATAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGGGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGgccag                                              <  1:831764/150‑1 (MQ=255)
                                                                                                              tGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGCCAGCCAgg                                         <  1:932384/150‑1 (MQ=255)
                                                                                                                gtcggtcCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGCCAGCCAGGCa                                       >  1:337030/1‑150 (MQ=255)
                                                                                                                 tcggtcCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGCCAGCCAGGCAg                                      <  1:519188/150‑1 (MQ=255)
                                                                                                                    gtcCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACGAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGCCAGCCAGGCAGCGt                                   >  1:861187/1‑150 (MQ=255)
                                                                                                                     tcCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGTGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGCCAGCCAGGCAGCGtt                                  <  1:126369/150‑1 (MQ=255)
                                                                                                                         aGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGCCAGCCAGGCAGCGTTAgtg                              <  2:1099640/150‑1 (MQ=255)
                                                                                                                         aGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGCCAGCCAGGCAGCGTTAgtg                              <  2:600816/150‑1 (MQ=255)
                                                                                                                         aGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGCCAGCCAGGCAGCGTTAgtg                              <  2:1300934/150‑1 (MQ=255)
                                                                                                                           cccGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGCCAGCCAGGCAGCGTTAGTGTc                            <  1:1221197/150‑1 (MQ=255)
                                                                                                                                     gtagtCAGCACGCCGATGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGCCAGCCAGGCAGCGTTAGTGTCAACGATTTGc                  >  1:1069535/5‑150 (MQ=255)
                                                                                                                                      cGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGCCAGCCAGGCAGCGTTAGTGTCAACGATTTGcc                 <  1:317577/150‑1 (MQ=255)
                                                                                                                                       gATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCACCCCCTGATGAAAGAAAAAATCGGCCCGCCAGCCAGCGTTAGTTGCACCGATTTGccc                >  2:596338/1‑150 (MQ=255)
                                                                                                                                        gtCAGCACGCCGATGTGCTGACTACGTTCGCGATGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCACCCCCTGATGAATGAAAAATTCGGCCAGCCAGGCAGCGTTAGGGTCACCGAGTTGCCCg               >  1:949564/2‑150 (MQ=255)
                                                                                                                                          cAGCACATCGGTGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGCCAGCCAGGCAGCGTTAGTGTCAACGATTTGCCCgtg             >  2:1298027/1‑150 (MQ=255)
                                                                                                                                           aGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGCCAGCCAGGCAGCGTTAGTGTCAACGATTTGCCCgtgt            >  1:515678/1‑150 (MQ=255)
                                                                                                                                                   ggCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGCCAGCCAGGCAGCGTTAGTGTCAACGATTTGCCCGTGTAACACTTc    <  2:749187/150‑1 (MQ=255)
                                                                                                                                                     cGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGCCAGCCAGGCAGCGTTAGTGTCAACGATTTGCCCGTGTAACACTTCAt  <  2:368301/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGCCGTTAACGATCAGCACATCGGCGTGCTGACTACGTTCGCGAAGAATGGTGACTAAGTCATCAAGGTTATCCCCCACCGTATTGCGGCGAGATAATGGCAACCCCTGATGAAAGAAAAAATCGGCCAGCCAGGCAGCGTTAGTGTCAACGATTTGCCCGTGTAACACTTCAT  >  NC_000913/2363304‑2363602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: