Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,594,273	C→T	5.9%	A529A (GCG→GCA)	tmcA ←	elongator methionine tRNA (ac4C34) acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,594,273	0	C	T	5.9%	217.8 / 2.2	69	A529A (GCG→GCA)	tmcA	elongator methionine tRNA (ac4C34) acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (30/34);  new base T (2/2);  total (32/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

CGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGC  >  NC_000913/2594131‑2594422                                                                                                                                               |                                                                                                                                                      ggtcgtcAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCg                                                                                                                                                <  1:545272/149‑1 (MQ=255)  gtcgtcAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGTGCCATCGc                                                                                                                                               >  1:644454/1‑150 (MQ=255)  gtcgtcAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGTGCCATCGc                                                                                                                                               >  1:594314/1‑150 (MQ=255)   tcgtcAGAAAGGACGGCATCGTTTAGTGGAGCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGcc                                                                                                                                              <  1:263511/150‑1 (MQ=255)    cgtcAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCg                                                                                                                                             >  1:149160/1‑150 (MQ=255)         gAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGCCGTAACCGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAg                                                                                                                                        >  2:104271/1‑150 (MQ=255)          aaaGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGc                                                                                                                                       <  1:1151171/150‑1 (MQ=255)            aGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCaa                                                                                                                                     >  2:1331142/1‑150 (MQ=255)              gACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAAcc                                                                                                                                   <  2:889176/150‑1 (MQ=255)                 ggCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTCCCCGCATCACTCATCGGTAACAGCGCCCTCGCCGTATAGCAACCgct                                                                                                                                >  2:99135/1‑150 (MQ=255)                  gCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCgctg                                                                                                                               >  1:123597/1‑150 (MQ=255)                  gCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCgctg                                                                                                                               >  1:843363/1‑150 (MQ=255)                       gTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGc                                                                                                                          <  2:888898/150‑1 (MQ=255)                       gTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGc                                                                                                                          <  2:1020398/150‑1 (MQ=255)                        tttAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCt                                                                                                                         >  2:972753/1‑150 (MQ=255)                           aGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCAGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTcc                                                                                                                      >  1:664016/1‑150 (MQ=255)                            gTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTccc                                                                                                                     >  2:514519/1‑150 (MQ=255)                             tGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGGCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCg                                                                                                                    <  2:234625/150‑1 (MQ=255)                              ggATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCga                                                                                                                   >  1:1281842/1‑150 (MQ=255)                                   aaCAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGGAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGAtt                                                                                                              <  1:836066/150‑1 (MQ=255)                                    aCAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTa                                                                                                             <  2:1083644/150‑1 (MQ=255)                                    aCAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTa                                                                                                             <  2:1089020/150‑1 (MQ=255)                                            cGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCg                                                                                                     <  1:523232/150‑1 (MQ=255)                                              tttCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCa                                                                                                   <  2:115588/150‑1 (MQ=255)                                                 cGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCa                                                                                                >  2:1213529/1‑150 (MQ=255)                                                           cTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAccc                                                                                      >  1:150103/1‑150 (MQ=255)                                                             gcgcGAGAGCTTGCGCATCGCGACGTAAACGGTACTGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGc                                                                                    <  2:1288491/150‑1 (MQ=255)                                                             gcgcGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGCTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGc                                                                                    <  2:58032/150‑1 (MQ=255)                                                               gcgAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAg                                                                                  >  1:1301481/1‑150 (MQ=255)                                                                cgAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGc                                                                                 >  2:1170597/1‑150 (MQ=255)                                                                  agagCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGt                                                                               >  1:57478/1‑150 (MQ=255)                                                                     gCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGc                                                                            >  2:435040/1‑150 (MQ=255)                                                                     gCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGc                                                                            >  2:343/1‑150 (MQ=255)                                                                     gCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGACAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCAGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGc                                                                            >  1:794407/1‑150 (MQ=255)                                                                       ttGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCa                                                                          <  2:772709/150‑1 (MQ=255)                                                                        tGCGCATCGCGACGTAAACTGTAATTCTCACGTTCAGCCAGCTGTTTACTCGCATCACTCATCGGTAACAGGGCCATCGACGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAg                                                                         <  2:529923/150‑1 (MQ=255)                                                                          cgcATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGaa                                                                       >  2:493150/1‑150 (MQ=255)                                                                             aTCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGTGCCATCGCCGTATAGCTACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACg                                                                    <  2:644454/150‑1 (MQ=255)                                                                             aTCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGTGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACg                                                                    <  2:594314/150‑1 (MQ=255)                                                                              tCGCGACGTAAACTGTAATGCTCACGTTAAGCCAGCTGTTTACCCGCATCACTCATCTGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTTCCATAAACGc                                                                   <  2:800795/150‑1 (MQ=255)                                                                              tCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGc                                                                   >  1:771416/1‑150 (MQ=255)                                                                              tCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGc                                                                   >  1:776660/1‑150 (MQ=255)                                                                                 cgACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTTCCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCAt                                                                <  2:792030/150‑1 (MQ=255)                                                                                 cgACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCAt                                                                <  2:500144/150‑1 (MQ=255)                                                                                        aaCGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTccc                                                         <  2:1067798/150‑1 (MQ=255)                                                                                          cGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCCCCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATACCTCCCCg                                                       >  2:1220697/1‑150 (MQ=255)                                                                                           ggTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCGGCACAACACCGCAGCGTTGCCAGAAACGCCATACCTCCCCgg                                                      >  1:240666/1‑150 (MQ=255)                                                                                           ggTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCgg                                                      <  2:776660/150‑1 (MQ=255)                                                                                           ggTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCgg                                                      <  1:972753/150‑1 (MQ=255)                                                                                           ggTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCgg                                                      <  2:771416/150‑1 (MQ=255)                                                                                             tAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGgtg                                                    <  1:937571/150‑1 (MQ=255)                                                                                             tAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGGTGCCAGAAACGCCATAACTCCCCGgtg                                                    <  2:732536/150‑1 (MQ=255)                                                                                              aaTGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGgtgt                                                   <  1:978325/150‑1 (MQ=255)                                                                                               aTGCTCACGTTCAGTCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTa                                                  >  2:547342/1‑150 (MQ=255)                                                                                                tGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTaa                                                 <  1:99135/150‑1 (MQ=255)                                                                                                 gCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAAc                                                <  2:1186483/150‑1 (MQ=255)                                                                                                   tCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCa                                              <  2:1043782/150‑1 (MQ=255)                                                                                                     aCGTTCAGCCAGCTGTTTACCCGCCTCACACCTCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCGGGCTTCCCGATGATTACCCATCCGCACCCGGCCAAACCCGCCGCGTTGCCAGGAACGCCAGTACTCCCCGGTGTAACCaaa                                            >  2:239452/1‑150 (MQ=255)                                                                                                       gTTCAGCCAGCGGTTTACCCGGATCACTCATCGGTATCAGCGCCATCGCCGTTTAGCAACCGCTGCTGGATTCCAGTTGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAAc                                          <  2:1186121/150‑1 (MQ=255)                                                                                                           agccagcTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTACCCAAAACTCAc                                      >  1:889837/1‑150 (MQ=255)                                                                                                           agccagcTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCAc                                      >  2:155886/1‑150 (MQ=255)                                                                                                                    tttACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATa                             <  1:1213529/150‑1 (MQ=255)                                                                                                                     ttACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAg                            <  2:843363/150‑1 (MQ=255)                                                                                                                        cccGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGATAGATAGTCg                         <  2:1012991/150‑1 (MQ=255)                                                                                                                         ccGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCCCCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCCCCGAAAGATAGTCGa                        >  1:13598/1‑150 (MQ=255)                                                                                                                          cGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCCCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAg                       <  2:123597/150‑1 (MQ=255)                                                                                                                                    aTCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGtt             >  2:1018955/1‑149 (MQ=255)                                                                                                                                       ggTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTatat          >  2:661894/1‑150 (MQ=255)                                                                                                                                        gTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATAtt         <  1:1296691/150‑1 (MQ=255)                                                                                                                                               cgcCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGc  <  1:1331142/150‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                      CGTCGTCAGAAAGGACGGCATCGTTTAGTGGATCAACAGGAAGCGTTTCGCCATTCCACTGCGCGAGAGCTTGCGCATCGCGACGTAAACGGTAATGCTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGC  >  NC_000913/2594131‑2594422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑