Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,804,225 G→A 5.8% A242T (GCC→ACC)  nrdF → ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,804,2250GA 5.8% 206.3 / 2.5 70A242T (GCC→ACC) nrdFribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein
Reads supporting (aligned to +/- strand):  ref base G (32/32);  new base A (2/2);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

AGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCG  >  NC_000913/2804077‑2804365
                                                                                                                                                    |                                                                                                                                            
aGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGc                                                                                                                                             <  2:1264573/150‑1 (MQ=255)
      ccACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAAc                                                                                                                                       >  1:1189101/1‑150 (MQ=255)
       cACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAAcc                                                                                                                                      >  2:79571/1‑150 (MQ=255)
            ttACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTg                                                                                                                                 <  2:790135/150‑1 (MQ=255)
            ttAATACATAGGCTATAAATATCAGAAAAACATGTAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTg                                                                                                                                 <  2:583493/150‑1 (MQ=255)
              actacATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTggg                                                                                                                               >  2:319180/1‑150 (MQ=255)
               ctacATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGc                                                                                                                              >  2:418564/1‑150 (MQ=255)
                tacATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCt                                                                                                                             <  2:448890/150‑1 (MQ=255)
                 acATAGGCTATAAATATCATAAAAACATGGAAAATATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCATTCGATTTGTTGCTGGAACTCTACGACATCGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGTGCTg                                                                                                                            <  2:1338934/150‑1 (MQ=255)
                  cATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGa                                                                                                                           <  1:1280139/150‑1 (MQ=255)
                      ggCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGAt                                                                                                                       >  1:1038995/1‑150 (MQ=255)
                      ggCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGAt                                                                                                                       >  1:1236795/1‑150 (MQ=255)
                        cTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGAtgt                                                                                                                     >  1:505983/1‑150 (MQ=255)
                           taAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGaa                                                                                                                  <  2:1093987/150‑1 (MQ=255)
                              atatCAGAAAAACATGGAAAAGATATCTATGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGCGCTGACGATGTGAAAGc                                                                                                               >  2:925745/1‑150 (MQ=255)
                                atCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGt                                                                                                             >  1:435648/1‑150 (MQ=255)
                                   aGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTtc                                                                                                          >  1:1099613/1‑150 (MQ=255)
                                    gAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGTGCTGACGATGTGATAGCGTTtct                                                                                                         >  1:247937/1‑150 (MQ=255)
                                    gAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTtct                                                                                                         >  1:273338/1‑150 (MQ=255)
                                        aaCATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGt                                                                                                     <  2:342096/150‑1 (MQ=255)
                                        aaCATGGAAAAGAAATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGt                                                                                                     <  2:1121176/150‑1 (MQ=255)
                                           aTGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGGTTCTCTGTTAc                                                                                                  <  2:1088379/150‑1 (MQ=255)
                                              gAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAAc                                                                                               <  1:79571/150‑1 (MQ=255)
                                                 aaGATATCTCTGGGAAAACGTGAAGAGTTTAAGAGATTCGCCTTCTATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACTCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGcc                                                                                            <  2:48330/150‑1 (MQ=255)
                                                       tctcTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGATGCAATACACCGATTAGCTGTACGCCGAAACCCCGTGGGCTCACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAg                                                                                      >  2:662199/1‑150 (MQ=255)
                                                         tctGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGc                                                                                    >  1:632337/1‑150 (MQ=255)
                                                             ggACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTCCGCCGAAACCCCGTGGGCTGACTCTGTGAAAGCGTGTCGCTGTTACAACTCCAATAAGGCTTtg                                                                                >  2:723433/1‑150 (MQ=255)
                                                             ggACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAACGCGTTTCTCTGTTACAACGCCAATAAGGCTTtg                                                                                >  2:718748/1‑150 (MQ=255)
                                                              gACAACGTGAAGAGTTTAAGATTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACAGCGATGAGCTGTACGCCGAAACCCCGTTGGCTGACGATGTGAAATCGTTTCTCTGTTACAACGCCAATAAGGCTTtga                                                                               <  1:796460/150‑1 (MQ=255)
                                                              gACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATGTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACAGATTAGCTGTACTCAGAAACCCCGTGTGCTGACGATGTGAAAGCGATACACTGATACAACGCCAAAAAGGCtttta                                                                               >  1:1318826/1‑148 (MQ=255)
                                                               aCAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTtgat                                                                              <  2:273338/150‑1 (MQ=255)
                                                                cAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTtgatg                                                                             <  2:1124703/150‑1 (MQ=255)
                                                                cAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTtgatg                                                                             >  2:706240/1‑150 (MQ=255)
                                                                      gaagagttgaagagttTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTg                                                                       >  1:696488/1‑150 (MQ=255)
                                                                          agttgaagagttTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCt                                                                   <  2:556653/150‑1 (MQ=255)
                                                                             tgaagagttTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACg                                                                >  2:1002138/1‑150 (MQ=255)
                                                                              gaagagttTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGa                                                               >  2:517871/1‑150 (MQ=255)
                                                                                 gagttTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAAcc                                                            >  2:1066878/1‑150 (MQ=255)
                                                                                 gagttTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAAcc                                                            >  2:412271/1‑150 (MQ=255)
                                                                                  agttTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCg                                                           <  2:1236795/150‑1 (MQ=255)
                                                                                     ttCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAAcc                                                            <  2:200048/146‑1 (MQ=255)
                                                                                     ttCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAAcc                                                            >  1:200048/1‑146 (MQ=255)
                                                                                          cTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTcc                                                   <  2:1299809/150‑1 (MQ=255)
                                                                                               aTTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAg                                              <  2:278229/150‑1 (MQ=255)
                                                                                                 ttggtgCTGGAACTCTACGCCAACGAGTTCCAATACACCGATGAGCTGTACGCCGAAACCCCGTTGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATCAGGCTTTGATGCATCTGGGCTACGAACCGTTATTTCCCGCAGaa                                            <  2:636054/150‑1 (MQ=255)
                                                                                                   gtggCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAAt                                          <  1:1323653/147‑1 (MQ=255)
                                                                                                     tgCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATgg                                        >  1:96268/1‑150 (MQ=255)
                                                                                                      gCTGGAACTCTACGACAACGAGTTGCAATACACCGATGCGCTGTCCGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTGTGATGCCTCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGc                                       <  1:831659/150‑1 (MQ=255)
                                                                                                      gCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACACCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGc                                       >  2:1274920/1‑150 (MQ=255)
                                                                                                      gCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACACCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGc                                       >  2:1269827/1‑150 (MQ=255)
                                                                                                       cTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCg                                      <  1:346180/150‑1 (MQ=255)
                                                                                                          gAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGaa                                   >  1:87231/1‑150 (MQ=255)
                                                                                                          gAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGaa                                   >  1:839255/1‑150 (MQ=255)
                                                                                                           aaCTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAg                                  <  1:584181/150‑1 (MQ=255)
                                                                                                              tctACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAgaga                               <  1:45177/150‑1 (MQ=255)
                                                                                                              tctACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGa                               <  2:1189101/150‑1 (MQ=255)
                                                                                                                  cGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACTCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATcc                           <  1:748085/150‑1 (MQ=255)
                                                                                                                  cGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATcc                           <  2:632337/150‑1 (MQ=255)
                                                                                                                   gACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCg                          >  2:1268359/1‑150 (MQ=255)
                                                                                                                     caccGAGTTGCAATACACCGATGAGCTGGACGCCGAAACCGCGTCGGCTGTCGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGc                        <  1:335508/147‑1 (MQ=255)
                                                                                                                        cGAGTTGCAATACACCGATGAGCTGTACACCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAAt                     <  1:1269827/150‑1 (MQ=255)
                                                                                                                        cGAGTTGCAATACACCGATGAGCTGTACACCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAAt                     <  1:1274920/150‑1 (MQ=255)
                                                                                                                          aGTTGCAATACACCTATTAGCGGTACGCCGAAACCCCGAGGGCTGACGATTTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATcc                   <  2:242126/150‑1 (MQ=255)
                                                                                                                          aGTTGCAATACACCGATGAGCTGTACGCCGAACCCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGACTCTGGGCTACGAACCGTTATTTCCCGCAGAAAGGGCGGAAGTGAATCCGGCAATcc                   <  1:1007127/150‑1 (MQ=255)
                                                                                                                                  tACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTACCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCcgcg           >  2:607637/1‑150 (MQ=255)
                                                                                                                                   acacCGATGAGCTGTACGCCGAAACCCAGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATTGCGGAAGTGAATCCGCCAATCCACGCCgagc          >  2:81257/1‑150 (MQ=255)
                                                                                                                                     acCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCtt        >  1:1089688/1‑150 (MQ=255)
                                                                                                                                       cGATGAGCTGTACGCCGAAACCCCGGGGGCTGCCGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGCATCTGGGCTACGCACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTc      >  2:875508/1‑150 (MQ=255)
                                                                                                                                          tGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGcc   >  1:611018/1‑150 (MQ=255)
                                                                                                                                           gAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCg  <  2:612765/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
AGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCG  >  NC_000913/2804077‑2804365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: