Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,804,528 C→A 14.2% intergenic (+67/‑287) nrdF → / → proV ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein/glycine betaine/proline ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,804,5280CA14.2% 172.7 / 10.2 78intergenic (+67/‑287)nrdF/proVribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein/glycine betaine/proline ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (41/24);  new base A (2/8);  total (43/35)
Fisher's exact test for biased strand distribution p-value = 1.53e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGG  >  NC_000913/2804379‑2804675
                                                                                                                                                     |                                                                                                                                                      
accACGATTTCTTTTCCGGTGCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATc                                                                                                                                                        <  2:696488/148‑1 (MQ=255)
aTCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATc                                                                                                                                                        >  2:501490/1‑150 (MQ=255)
 tCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCa                                                                                                                                                       <  1:607637/150‑1 (MQ=255)
   aCGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACg                                                                                                                                                     >  1:1109997/1‑150 (MQ=255)
   aCGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACg                                                                                                                                                     <  1:319180/150‑1 (MQ=255)
    cGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGCAAATAATTCCCGCCAAATAGCTTTTTATCACGc                                                                                                                                                    >  2:1050816/1‑150 (MQ=255)
             ttCCGGTTCAGCCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGATATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCCCAAAATAGCTTTTTATCACGCAAATAAttt                                                                                                                                        >  1:393723/1‑150 (MQ=255)
               ccGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAATTATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTtgt                                                                                                                                      <  1:925745/150‑1 (MQ=255)
                 ggTTCAGGCTCCTCTTATGTGATGTGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCATCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTgtgg                                                                                                                                    <  1:517871/150‑1 (MQ=255)
                  gTTCAGGCTCCTCTTATGTGATGGTGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTgtggt                                                                                                                                   <  2:96268/150‑1 (MQ=255)
                   ttCATGCTCCTCTTATGTGATGGGGAAAGCGTTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGTAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTgtggtg                                                                                                                                  <  2:868912/150‑1 (MQ=255)
                   ttCAGGCTCCTCTTTTGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTgtggtg                                                                                                                                  <  2:1286708/150‑1 (MQ=255)
                   tgaaGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTgtggtg                                                                                                                                  <  2:192638/147‑1 (MQ=255)
                    tCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGAAGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCACCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGa                                                                                                                                 <  2:611018/150‑1 (MQ=255)
                     cAGGCGCCTCATCTGTGATGGCGCAAGCGGTTGAAACAGAAGTTGTAGACTGGTATTTCTGAGGGTTTTATTTTCAAAAATTTCACTACCCGCAGCAGGGAAATAATTCCCGCCACATAGCTTTTTATCACGCAAATAATTTGTGGTGAt                                                                                                                                <  2:130968/150‑1 (MQ=255)
                            ctctTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACt                                                                                                                         >  2:1045271/1‑150 (MQ=255)
                                tATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATAACGCAAATAATTTGTGGTGATCTACACTGATa                                                                                                                     >  1:286656/1‑150 (MQ=255)
                                 aTGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATAc                                                                                                                    <  1:1040865/150‑1 (MQ=255)
                                   gtgATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATTTTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATActc                                                                                                                  <  2:701776/150‑1 (MQ=255)
                                   gtgATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTCATActc                                                                                                                  >  2:807418/1‑150 (MQ=255)
                                    tgATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACTCAAATAATATGTGGTGATCTACACTGATActct                                                                                                                 >  1:177180/1‑150 (MQ=255)
                                    tgATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTAACACCCAAATAATTTGTGGTCATAAACACTGATActct                                                                                                                 >  1:425457/1‑150 (MQ=255)
                                    tgATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCAGCAGCAGGGAAATAATTACAGCCAAATAGCTTTTTATCACGCAAATAATTTGTGATGATCTACACTTATAatct                                                                                                                 >  1:934669/1‑150 (MQ=255)
                                       tGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGtt                                                                                                              >  1:83205/1‑150 (MQ=255)
                                            aaatctgTTGAAACAGAACAAGACCCCTCGCAATTCTGAGGGTGTTATTTTCAAAAATAGCACTACCCGCATCAGGGAAATAATTCCCTCCAAATATTTTTTTTTATGTTAATTATATTTTGGAGATCTACACTGATACTCTGTTGCatt                                                                                                         <  2:1172293/144‑1 (MQ=255)
                                             aaGCGGTTGAAACAGAAGATGAAGACTGTAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGAGCTACACTGATACTCTGTTGCatta                                                                                                        <  1:967729/150‑1 (MQ=255)
                                             aaGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCatta                                                                                                        <  1:1239576/150‑1 (MQ=255)
                                             aaGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCatta                                                                                                        <  1:541714/150‑1 (MQ=255)
                                                   ttGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGcc                                                                                                  <  1:341043/150‑1 (MQ=255)
                                                   ttGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGcc                                                                                                  <  1:1302078/150‑1 (MQ=255)
                                                     gAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTg                                                                                                >  1:394642/1‑150 (MQ=255)
                                                     gAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGAATTATTCGCCTg                                                                                                >  1:909476/1‑150 (MQ=255)
                                                         cAGAAGATGAAGACTGTAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCACGCTAATTTTTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAAc                                                                                            <  2:1101734/150‑1 (MQ=255)
                                                         cAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCAGTAAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAAc                                                                                            <  2:1109849/150‑1 (MQ=255)
                                                           gaagaTGAAGACTGGAATGTCTGAGGGTGTTATTTTCATAAATAGCACTACCCGCAGCAGGCAAATAATTCCCGCCAATTAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTTCATTATTCGCCTGAAAccc                                                                                          >  1:77043/1‑149 (MQ=255)
                                                             agaTGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACcaca                                                                                        >  1:1241814/1‑150 (MQ=255)
                                                              gaTGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCATGGAAATAATTCCAGACAATTTTTTTTTTTTATGTTAATTATATTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACaa                                                                                       <  2:1050929/150‑1 (MQ=255)
                                                              gaTGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACcaca                                                                                        >  2:782765/1‑150 (MQ=255)
                                                               aTGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAat                                                                                      >  2:802562/1‑150 (MQ=255)
                                                                tGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACTCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAata                                                                                     >  2:539220/1‑150 (MQ=255)
                                                                    gACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAAATCCCGCCAAATATCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGAATTATTCTCCTGAAACCACAATAATCa                                                                                 >  2:1179506/1‑150 (MQ=255)
                                                                        ggAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATAACGCAAATAATTTGTTGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCg                                                                             >  2:479054/1‑150 (MQ=255)
                                                                          aaTTTCTGAGGGGGGTATATTCAAAAATATCACTACCCGCAGCAGGTAATTAATAGCAGACTTTTATTTTTTTTTATGTTAATTATTTTTTCGTGATCTAAACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGtg                                                                           <  2:648418/150‑2 (MQ=255)
                                                                                tGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGACTGAAACCACAATATTCAGGCGTTTTTTCg                                                                     >  1:779728/1‑150 (MQ=255)
                                                                                  aGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCAGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTAGCt                                                                   >  2:266610/1‑150 (MQ=255)
                                                                                       gtTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCtt                                                              <  1:1096717/150‑1 (MQ=255)
                                                                                        ttATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAATCCACAATATTCAGGCGTTTTTTCGCTATCttt                                                             >  1:1281463/1‑150 (MQ=255)
                                                                                         tATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTg                                                            >  1:33796/1‑150 (MQ=255)
                                                                                          aTTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGATTTTTATATGGTAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGa                                                           <  2:1235039/150‑1 (MQ=255)
                                                                                           ttttCAAAAATATCACTACCCGCAGCAGGGAAATATTTCCCGACAAATTTTTTTTTTTATGTTAATGATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGAc                                                          <  2:729664/150‑1 (MQ=255)
                                                                                           ttttCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATTTTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGAc                                                          <  2:291284/150‑1 (MQ=255)
                                                                                            tttCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACa                                                         >  2:906960/1‑150 (MQ=255)
                                                                                             ttCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACaa                                                        >  1:544542/1‑150 (MQ=255)
                                                                                              tCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACaaa                                                       <  1:1334450/150‑1 (MQ=255)
                                                                                               cAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACaaaa                                                      >  2:1339724/1‑150 (MQ=255)
                                                                                               cAAAAATATCACTACCCGCAGCAGGGAAATAATTACCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACaaaa                                                      >  2:251865/1‑150 (MQ=255)
                                                                                                 aaaaTATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACaaaaaa                                                    >  2:550290/1‑150 (MQ=255)
                                                                                                   aaTATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTTATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTATCGCTATCTTTTACAAAAAata                                                  >  2:99611/1‑150 (MQ=255)
                                                                                                   aaTATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATTTTTGACAAAAAata                                                  >  1:35669/1‑150 (MQ=255)
                                                                                                     tatCACTACCCGCAGCAGGGAAATAATTACCGACAAATTTTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATc                                                <  2:286656/150‑1 (MQ=255)
                                                                                                      atCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCa                                               >  2:909484/1‑150 (MQ=255)
                                                                                                      atCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTAACACGCAAATAATTTGTCGTGATCTACACTGAAACTCTGTTGCATTATTCGCCTGAAACCACAAAATTCACGCGTTTTTTCGCTAACATTGACAAAAAATATCa                                               >  2:579704/1‑150 (MQ=255)
                                                                                                         aCTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGAGTTTTTTCGCTATCTTTGACAAAAAATATCAACt                                            >  2:1265005/1‑150 (MQ=255)
                                                                                                          caaCCCGCATCAGTGAAATAATTCCCGCCAAATTTTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACtt                                           <  2:792394/148‑1 (MQ=255)
                                                                                                             cccGCAGCAGGGAAATAATTCCCTCCAAATATATTTTTATCACCCAAATAATTTGTGGTGATCTACACTGATACTCTGTTTCATTATTCGACTGAAACCACAAAATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTtct                                        >  2:77867/1‑150 (MQ=255)
                                                                                                              ccGCAGCAGGGAAATAATTCCCGCCAAATAGATTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTtctc                                       <  2:1109997/150‑1 (MQ=255)
                                                                                                                    cagGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTtctc                                       >  2:118849/1‑144 (MQ=255)
                                                                                                                    cagGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTtctc                                       <  1:118849/144‑1 (MQ=255)
                                                                                                                        gAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATAATCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTAGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGatct                             >  2:1260871/1‑150 (MQ=255)
                                                                                                                         aaaTAATTCCCGCCAAATATCTTTTTATCACTAAATTAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCtctc                            <  2:1241814/150‑1 (MQ=255)
                                                                                                                              atTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGccc                       >  2:594970/1‑150 (MQ=255)
                                                                                                                               ttCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTTATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCt                      <  1:1350262/150‑1 (MQ=255)
                                                                                                                                 cccGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTta                    >  1:285403/1‑150 (MQ=255)
                                                                                                                                 cccGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTta                    >  1:1255337/1‑150 (MQ=255)
                                                                                                                                     ccAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATc                >  2:375615/1‑150 (MQ=255)
                                                                                                                                     ccAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACAATGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTAAGTCGTTTTTACCCTATCTTTGACAAAAAATATAAACTTTATCGATTTAATCTCAACAATTATATc                >  2:433088/1‑150 (MQ=255)
                                                                                                                                         aTAGATTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACgg            <  2:1063675/150‑1 (MQ=255)
                                                                                                                                              tttttATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGACACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGAGCTCAGCCCTTATATCACGGGAAAt       >  2:207010/1‑150 (MQ=255)
                                                                                                                                               ttttATATCGTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGTCCTTATATCACGGGAAAtt      <  2:779728/150‑1 (MQ=255)
                                                                                                                                                   aTCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCgg  >  2:806792/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
ATCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGG  >  NC_000913/2804379‑2804675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: